Life Science Buffers
Balanced salt solutions and powders used for molecular, protein, nucleic acid, and cell biology applications including cell culture, electrophoresis, ELISA, and chromatography processes; available in a variety of formulations and grades.
Buffer solutions serve to keep pH (acidity or alkalinity) nearly constant in a variety of chemical and biological applications. For example, a bicarbonate buffer helps maintain the pH of blood, a buffered saline maintains cellular contents at a consistent pH level, and buffers help maintain a narrow pH range for enzymes to function correctly.
Most biological samples used in research are kept at a pH of about 7.4. Common biological buffers used for tissue culture include Dulbeccos phosphate buffered saline (PBS), Tris base, HEPES, MOPS, PIPES, and other formulations developed for specific cell lines or applications. Most buffers used in cell culture are also DNase-, RNase-, and protease-free.
The choice of buffer for a particular biological reaction or site depends on several factors:
- Temperature
- Desired or target pH
- Buffer toxicity (to the system)
- Buffer interactions with other system components
Biotang Inc Carbonate Buffered Saline 5X, pH 9.5. 15 mM Sodium Carbonate, 750 mM Sodium Chloride, pH 9.5. Prepared in 18.2 megohms water and filtered through 0.22 micron filter. BB-535
Carbonate Buffered Saline 5X, pH 9.5. 15 mM Sodium Carbonate, 750 mM Sodium Chloride, pH 9.5. Prepared in 18.2 megohms water and filtered through 0.22 micron filter. BB-535.
![Alkali Scientific MOPS, Sodium Salt [3 (N Morpholino) propanesulfonic acid sodium salt], 50 G](https://assets.fishersci.com/TFS-Assets/CCG/product-images/5000660066.jpg-250.jpg)
Alkali Scientific MOPS, Sodium Salt [3 (N Morpholino) propanesulfonic acid sodium salt], 50 G
MOPS (3-(N-Morpholino)propanesulfonic acid sodium salt) is a versatile biochemical reagent used for pH buffering in various research applications. This 50g bottle provides a compact quantity of MOPS sodium salt, ideal for laboratories with moderate buffer preparation needs. MOPS is widely used in molecular biology, biochemistry, and biotechnology for stabilizing pH in enzyme reactions, protein assays, and cell culture. Its pH buffering capacity at a range of 7.0–7.5 is particularly beneficial in experiments where a consistent environment is essential. MOPS also serves as an essential reagent in electrophoresis, chromatography, and other scientific procedures that demand precise pH control.
![Alkali Scientific HEPES, Free Acid [N (2 Hydroxyethyl) piperazine N' (2 ethanesulfonic acid)], 5 Kg](https://assets.fishersci.com/TFS-Assets/CCG/product-images/5000660276.jpg-250.jpg)
Alkali Scientific HEPES, Free Acid [N (2 Hydroxyethyl) piperazine N' (2 ethanesulfonic acid)], 5 Kg
HEPES, Free Acid is a powerful biochemical reagent provided in a 5kg size, ideal for large-scale applications in biological and biochemical research. It helps maintain a stable pH in the range of 6.8–8.2, which is critical for a variety of assays, including cell culture, protein purification, and enzyme assays. The reagent's excellent buffering capacity ensures that pH remains stable throughout experiments, preventing fluctuations that could affect results. Its low toxicity and high stability make it particularly useful in biological systems, offering consistent performance and reproducible results for laboratories involved in cell-based studies and biochemical analysis.
Medchemexpress LLC 2-Morpholinoethanesulphonic acid potassium | 39946-25-3 | 99.9% | 50 G
MES potassium is an amphoteric ion buffer widely used in biochemistry and molecular biology experiments. It provides effective buffering capacity around pH 6.15 and is suitable for cell culture, enzyme activity determination, electrophoresis, and protein studies.
- Amphoteric ion buffer
- Features a buffer capacity ranging from pH 5.5-7.0
- Functions as a zwitterionic buffer
- Exhibits good buffering properties in biological experiments
- Shows poor affinity with metal ions, suitable for metal ion studies
Biotang Inc HEPES Buffer 0.5M, pH 8.0, 100ml, 500 mM HEPES (4-(2-Hydroxyethyl)piperazine-1-ethanesulfonic acid), pH 8.0 +/- 0.15. BB-2080
HEPES Buffer 0.5M, pH 8.0, 100ml, 500 mM HEPES (4-(2-Hydroxyethyl)piperazine-1-ethanesulfonic acid), pH 8.0 +/- 0.15. BB-2080.
Bioworld BES (2-(Bis(2-hydroxyethyl) amino) ethanesulfonic acid) Buffer, 0.5M, pH 7.0, 250 mL
Synonym:N,N-Bis(2-hydroxyethyl)-2-aminoethanesulfonic acid sodium salt, BES sodium saltBelongs to the class of Good buffers, as described in the research of Dr. Norman Good et al. (1966), optimized for molecular biology and biochemistry applications. The characteristics of a Good′s buffer include: pKa between 6.0 and 8.0, high solubility, non toxic, limited effect on biochemical reactions, very low absorbance between 240-700 nm, enzymatic and hydrolytic stability, minimal changes due to temperature and concentration, limited effects due to ionic or salt composition of the solution, limited interaction with mineral cations and limited permeability of biological membranes.Technical Information :Useful pH range :6.4 - 7.8pKa (25 °C) :7.1 (Free acid)Warning:For laboratory use only.Not fit for agricultural, household, clinical use or human consumption.
ILEX LIFE SCIENCES LLC PAN Biotech HEPES Buffer 1 M GMP Grade Cell Culture Buffer 100 ML 1/EA
PAN-Biotech HEPES Buffer 1 M GMP Grade 100 ml INTRODUCTION HEPES is a zwitterionic sulfonic acid buffering agent widely used in cell culture largely because it is better at maintaining physiological pH despite changes in carbon dioxide concentration (produced by aerobic respiration) when compared to bicarbonate buffers GMP GRADE PAN-Biotech HEPES Buffer 1 M GMP Grade is a ready-to-use highly concentrated buffer solution designed for direct use in GMP-regulated clinical and industrial applications The GMP grade version is based on the proven HEPES formulation and is manufactured under strictly controlled GMP conditions Defined raw materials high batch-to-batch consistency and comprehensive documentation make HEPES Buffer 1 M GMP a true ready-to-use solution suitable for immediate use in sensitive cell culture and manufacturing workflows COMPOSITION HEPES 238 31 g/L QUALITY GMP Grade (EU Annex 1) LIQUID/POWDER Liquid STERILE Yes STORAGE 2-8 C
![Alkali Scientific POPSO, Free Acid, [Piperazine N N' bis(2 hydroxypropane sulfonic acid)], 100 G](https://assets.fishersci.com/TFS-Assets/CCG/product-images/5000661550.jpg-250.jpg)
Alkali Scientific POPSO, Free Acid, [Piperazine N N' bis(2 hydroxypropane sulfonic acid)], 100 G
POPSO (Free Acid) is a high-purity biochemical reagent, available in a 100g package, and is commonly used in laboratory research for buffer preparation and pH stabilization. This compound is specifically designed for use in biochemical assays and molecular biology experiments where a stable pH is critical. POPSO, also known as Piperazine N,N' bis(2 hydroxypropane sulfonic acid), is used in studies that require an effective buffering agent to maintain physiological pH conditions. It is especially useful in protein analysis, enzyme reactions, and cell culture experiments. The 100g package size is ideal for smaller-scale applications and academic research, providing an accurate and reliable reagent for laboratory procedures.
![Alkali Scientific DIPSO [3 (N,N Bis(2 Hydroxyethyl)amino) 2 hydroxypropane sulfonic acid], 100 G](https://assets.fishersci.com/TFS-Assets/CCG/product-images/5000661848.jpg-250.jpg)
Alkali Scientific DIPSO [3 (N,N Bis(2 Hydroxyethyl)amino) 2 hydroxypropane sulfonic acid], 100 G
DIPSO (3 [N,N-Bis(2 Hydroxyethyl)amino] 2-hydroxypropane sulfonic acid) is a biochemical reagent supplied in a 100g bottle. This buffering agent is commonly used in biochemistry and molecular biology applications. DIPSO is known for its ability to maintain a stable pH in aqueous solutions, particularly at low pH values, making it ideal for use in enzyme assays, protein purification, and other chemical reactions that require precise pH control. It is often employed in applications such as electrophoresis, chromatography, and protein studies. This high-quality reagent is suitable for various laboratory protocols that demand reliable buffering capacity and pH stability.
Medchemexpress LLC L-Adenosine | 3080-29-3 | 99.8% | 1 ML
L-Adenosine is a metabolically stable enantiomeric analog and a potential probe. It exhibits weakly inhibitory adenosine deaminase (ADA) activity with a Ki value of 385 μM. This compound can be utilized for research into adenosine uptake and accumulation.
- Metabolically stable enantiomeric analog.
- Potential probe for research.
- Weakly inhibits adenosine deaminase activity.
- Can be used for adenosine uptake research.
- Can be used for adenosine accumulation research.