Life Science Buffers
Balanced salt solutions and powders used for molecular, protein, nucleic acid, and cell biology applications including cell culture, electrophoresis, ELISA, and chromatography processes; available in a variety of formulations and grades.
Buffer solutions serve to keep pH (acidity or alkalinity) nearly constant in a variety of chemical and biological applications. For example, a bicarbonate buffer helps maintain the pH of blood, a buffered saline maintains cellular contents at a consistent pH level, and buffers help maintain a narrow pH range for enzymes to function correctly.
Most biological samples used in research are kept at a pH of about 7.4. Common biological buffers used for tissue culture include Dulbeccos phosphate buffered saline (PBS), Tris base, HEPES, MOPS, PIPES, and other formulations developed for specific cell lines or applications. Most buffers used in cell culture are also DNase-, RNase-, and protease-free.
The choice of buffer for a particular biological reaction or site depends on several factors:
- Temperature
- Desired or target pH
- Buffer toxicity (to the system)
- Buffer interactions with other system components
Bioworld TT - SDS Running Buffer (Cathode) (10X), 500 mL
Product DescriptionTris-Tricine (TT)-SDS running buffer has a 10X concentration and a pH of 8.3. It contains 1M Tris-base, 1M Tricine and 1% SDS.The tricine system requires SDS in sample and running buffers for best results. Tricine-SDS PAGE is commonly used to separate proteins that range in molecular weight between 1 – 100 kDa but is preferred for the resolution of proteins smaller than 30 kDa.
TARGETMOL CHEMICALS INC NVP-TAE 226 5MG
Also available in 1 mL, 1 mg, 2 mg, 10 mg, 25 mg, 50 mg, 100 mg and bulk. Please contact Fisher for quotes. NVP-TAE 226 (TAE226) is a potent FAK inhibitor (IC50 5.5 nM), exhibiting even greater efficacy against Pyk2 (IC50 3.5 nM), and is 10- to 100-fold less effective against IGF-1R, InsR, c-Met, and ALK. Purity 98.07%
Enzo Life Sciences Ada-Tyr-(Ahx)3-(Leu)3-vinyl sulfone (100µg)
A highly potent, cell-permeable proteasome inhibitor that targets all individual active subunits with comparable affinity. This extended peptide-based inhibitor can be radioiodinated, allowing detection of labeled subunits by autoradiography. Purity: ≥97%. Long Term Storage: -20°C.
Kd Medical Inc TRIS-GLYCINE BUFFER 10X
10X Tris Glycine is used for electrophoretic transfer of protein from polyacrylamide gels to nitrocellulose sheets. 10X concentrated Tris Glycine is ready to dilute to a 1x working solution. One 1liter bottle.
Aniara Diagnostica LLC PBS PDR PH 7.4 10L
Medicago Phosphate Buffered Saline (PBS) pH 7.4 supplied as preweighed powder mix in sealed pouch yields 10 L 0.14 M NaCl, 0.0027 M KCl, 0.010 M phosphate buffer with pH 7.4 at 25C. Used as biological buffer, isotonic. 1 pouch.
