Life Science Buffers
Balanced salt solutions and powders used for molecular, protein, nucleic acid, and cell biology applications including cell culture, electrophoresis, ELISA, and chromatography processes; available in a variety of formulations and grades.
Buffer solutions serve to keep pH (acidity or alkalinity) nearly constant in a variety of chemical and biological applications. For example, a bicarbonate buffer helps maintain the pH of blood, a buffered saline maintains cellular contents at a consistent pH level, and buffers help maintain a narrow pH range for enzymes to function correctly.
Most biological samples used in research are kept at a pH of about 7.4. Common biological buffers used for tissue culture include Dulbeccos phosphate buffered saline (PBS), Tris base, HEPES, MOPS, PIPES, and other formulations developed for specific cell lines or applications. Most buffers used in cell culture are also DNase-, RNase-, and protease-free.
The choice of buffer for a particular biological reaction or site depends on several factors:
- Temperature
- Desired or target pH
- Buffer toxicity (to the system)
- Buffer interactions with other system components
Bioworld ACE MES Buffer, 50mM, pH 6.0, 250 mL
Product DescriptionA.C.E.™ MES (compound 2-(N-morpholino)ethanesulfonic acid) buffer has a concentration of 50 mM with a pH level of 6.0. The buffer is biotechnology grade and is formulated for capillary electrophoresis. Buffer is made with 3D water and filtered through 0.22 µ filter. Product is shipped at room temperature.
Medchemexpress LLC 2-Morpholinoethanesulphonic acid hydrate | 1266615-59-1 | 99.9% | 50 G
MES hydrate is an amphoteric ion buffer with a buffering capacity ranging from pH 5.5-7.0. It is widely used in biochemistry and molecular biology experiments for applications such as cell culture, enzyme activity determination, electrophoresis, and protein studies.
- Provides effective buffering capacity at approximately pH 6.15.
- Its pKa value changes with temperature, necessitating consideration for different experimental conditions.
- Exhibits good buffering properties in biological experiments.
- Shows poor affinity with metal ions, allowing its use in studies involving them.
Selleck Chemical LLC HEPES
HEPES is a nonvolatile zwitterionic chemical buffering agent which is broadly applied in cell culture HEPES is effective at pH 6 8 to 8 2 HEPES is also a potent inducer of lysosome biogenesis
Boston Bioproducts Inc HEPES Buffer (1 M, pH 7.5, pH Adjusted with KOH) - 250ml
The HEPES [4-(2-hydroxyethyl)-1-piperazineethanesulfonic acid)] Buffer is a versatile Good's zwitterionic buffering agent widely used in various applications in biochemistry and molecular biology. It maintains a stable pH in the range of 6.8 to 8.2, making it ideal for various applications at physiological pH, including cell culture, protein purification, isoelectric focusing, enzyme studies, bioprocessing, and live cell imaging. This buffer is adjusted with KOH.
Selleck Chemical LLC Bicine
Bicine (N N-Bis(2-hydroxyethyl)glycine Diethylolglycine Diethanol glycine Dihydroxyethylglycine) is an organic compound used as a buffering agent
![Alkali Scientific MOPS, Sodium Salt [3 (N Morpholino) propanesulfonic acid sodium salt], 1 Kg](https://assets.fishersci.com/TFS-Assets/CCG/product-images/5000660034.jpg-250.jpg)
Alkali Scientific MOPS, Sodium Salt [3 (N Morpholino) propanesulfonic acid sodium salt], 1 Kg
MOPS (3-(N-Morpholino)propanesulfonic acid sodium salt) is a pH buffering reagent commonly used in biological and chemical research. This 1 kilogram bottle provides a larger quantity of MOPS sodium salt for high-volume applications requiring consistent pH stability. It is ideal for maintaining physiological pH in biochemical and biological experiments, such as enzyme assays, protein analyses, and cell culture maintenance. The reagent is particularly useful in experiments that involve prolonged reaction times, as it ensures that pH remains within a narrow, optimal range for sensitive biological systems. MOPS is also used in molecular biology for electrophoresis and other lab protocols that require precise pH control.