Life Science Buffers
Balanced salt solutions and powders used for molecular, protein, nucleic acid, and cell biology applications including cell culture, electrophoresis, ELISA, and chromatography processes; available in a variety of formulations and grades.
Buffer solutions serve to keep pH (acidity or alkalinity) nearly constant in a variety of chemical and biological applications. For example, a bicarbonate buffer helps maintain the pH of blood, a buffered saline maintains cellular contents at a consistent pH level, and buffers help maintain a narrow pH range for enzymes to function correctly.
Most biological samples used in research are kept at a pH of about 7.4. Common biological buffers used for tissue culture include Dulbeccos phosphate buffered saline (PBS), Tris base, HEPES, MOPS, PIPES, and other formulations developed for specific cell lines or applications. Most buffers used in cell culture are also DNase-, RNase-, and protease-free.
The choice of buffer for a particular biological reaction or site depends on several factors:
- Temperature
- Desired or target pH
- Buffer toxicity (to the system)
- Buffer interactions with other system components
Boston Bioproducts Inc HBS-P+ Buffer (10X, pH 7.4) - 4x500ml
HBS-P Plus Buffer (10X pH 7 4) - 4x500ml (For Research Use and Further Manufacturing Only)
Boston Bioproducts Inc Phosphate Buffered Saline, PBS (10X, RNase Free) - 250ml
Phosphate Buffered Saline PBS (10X RNase Free) - 250ml (For Research Use and Further Manufacturing Only)
Boston Bioproducts Inc Tris-Acetate Buffer (0.2 M, pH 7.8) - 500ml
Tris-Acetate Buffer (0 2 M pH 7 8) - 500ml (For Research Use and Further Manufacturing Only)
Boston Bioproducts Inc HBS-P+ Buffer (1X, pH 7.4) - 4x500ml
HBS-P Plus Buffer (1X pH 7 4) - 4x500ml (For Research Use and Further Manufacturing Only)
Kd Medical Inc PBS Solution With Sodium Azide, 500 mL, Antimicrobial Preservative
PBS W/SODIUM AZIDE 500ML is a buffered saline solution designed for laboratory applications requiring a preservative to inhibit microbial growth. This product not only provides a stable wash and storage buffer but also enhances the longevity of biological samples. The inclusion of sodium azide ensures effective preservation, differentiating it from standard saline solutions. This versatile product is essential for researchers seeking reliable and long-lasting buffer solutions.
Sigma Aldrich Fine Chemicals Biosciences Phosphate buffered saline tablet, MFCD00131855
Phosphate buffered saline tablet
Thermo Scientific Production Chemicals and Services DOE AND INGALLS
NC3959187 ELUTE BUFFER A 50MM TRIS HCL8
Teknova 1M Tris-HCl, pH 9.0. 500 mL, Sterile. 4 pack.
Tris (tris(hydroxymethyl)aminomethane) is used as a component ofbuffer solutions, especially for solutions ofnucleic acids. The useful buffer range for tris (pH 7–9) is similar to the pH of most living organisms. This, and its low cost, make tris one of the most common buffers in the biology/biochemistry laboratory.
Teknova 1M Tris-HCl, pH 8.3. 500mL, Sterile. 4 pack.
Tris (tris(hydroxymethyl)aminomethane) is used as a component ofbuffer solutions, especially for solutions ofnucleic acids. The useful buffer range for tris (pH 7–9) is similar to the pH of most living organisms. This, and its low cost, make tris one of the most common buffers in the biology/biochemistry laboratory.
Research Products International Corp PBS 200ml Tablets 100 Tab
PBS, 200ml Tablets refer to pre-weighed tablets used to prepare a specific volume of PBS (Phosphate Buffered Saline) solution.
The buffer tablets are commonly used buffer solution in biological and biochemical research. PBS serves as a versatile buffer for various applications, including cell culture, immunohistochemistry, protein and nucleic acid research, and diagnostic assays. It helps maintain a stable pH and osmolarity, providing optimal conditions for biological reactions and preserving the integrity of cells and biomolecules.
The PBS, 200ml Tablets are designed to simplify the preparation process of PBS solutions. Each tablet contains pre-determined amounts of sodium chloride, sodium phosphate dibasic, and potassium phosphate monobasic. By using these tablets, researchers can save time and ensure accuracy by eliminating the need to weigh and measure individual components.
Thermo Scientific Production Chemicals and Services DOE AND INGALLS
NC3959194 10X ADJ BUF 1.0M NACL .5M THCL
Apexbio Technology LLC AO/PI Double Staining Kit 100T
The AO/PI Double Staining Kit is a reagent kit enabling rapid detection of cell viability This kit provides two nuclear dyes Acridine Orange (AO) and Propidium Iodide (PI) AO can penetrate intact cell membranes and stain viable cells green During apoptosis chromosomes become condensed or fragmented into differently sized segments In this scenario AO stains apoptotic cells densely with yellow-green fluorescence or as yellow-green fragments The other nuclear dye PI cannot pass through intact cell membranes therefore it does not stain viable or apoptotic cells in red but stains cells with compromised membranes (e g necrotic cells) When using these two dyes in combination viable cells exhibit green fluorescence apoptotic cells show dense varied-size yellow-green or orange fluorescence and necrotic cells exhibit strong red fluorescence
Apexbio Technology LLC Live-Dead Cell Staining Kit I (Calcein AM/PI) 100T
Calcein AM/PI Live-Dead Cell Staining Kit is a reagent kit used to distinguish between viable mammalian cells with esterase activity and dead cells The kit contains two probes Calcein AM and Propidium Iodide (PI) Calcein AM is a probe that stains live cells it is a derivative of Calcein with an added AM moiety which makes it more hydrophobic and allows better cell permeability Calcein AM itself is non-fluorescent but once it enters live cells it is hydrolyzed by intracellular esterases into Calcein which can emit strong green fluorescence PI is a probe for dead cells which enters only cells with compromised membranes staining them red The fluorescence intensities of these two probes reflect cell viability and toxicity The kit is easy to use 30 minutes of staining incubation After staining results can be detected using fluorescence microscopy fluorescence microplate readers or flow cytometry This kit is unsuitable for bacteria or fungi
Apexbio Technology LLC One-step TUNEL FITC Apoptosis Detection Kit 20rxns
One-step TUNEL FITC Apoptosis Detection Kit can be used to detect nuclear DNA fragmentation in tissues or cells undergoing apoptosis During apoptosis intracellular DNA endonucleases become activated cleaving genomic DNA between nucleosomes into 180-200 bp fragments or multiples thereof The resulting 3 -OH DNA ends are labeled with tagged dUTP through the catalytic action of Terminal Deoxynucleotidyl Transferase (TdT) allowing detection via fluorescence microscopy or flow cytometry with excitation/emission wavelengths at Ex/Em 492 nm/517 nm This kit has broad application and can be used to detect apoptosis in frozen or paraffin-embedded tissue sections as well as in cultured adherent or suspension cells
Apexbio Technology LLC Protease Inhibitor Cocktail (EDTA-Free 200X in DMSO) 100x1ml
When extracting secreted proteins from culture medium in vitro protein production ceases and degradation increases Cell crude extracts contain many endogenous enzymes such as phosphatases and proteases capable of degrading proteins in the extracts The best way to improve the yield of intact proteins is to add inhibitors targeting these known enzymes The Protease Inhibitor Cocktail is used in tissue culture media to enhance protein stability It functions by inhibiting proteases responsible for the degradation of both phosphorylated and non-phosphorylated protein substrates This cocktail is provided as a supplement for tissue culture media to prevent degradation of secreted proteins This Protease Inhibitor Cocktail has been optimized and tested specifically for tissue culture media This protease inhibitor cocktail is provided in DMSO as a ready-to-use solution