Life Science Buffers
Balanced salt solutions and powders used for molecular, protein, nucleic acid, and cell biology applications including cell culture, electrophoresis, ELISA, and chromatography processes; available in a variety of formulations and grades.
Buffer solutions serve to keep pH (acidity or alkalinity) nearly constant in a variety of chemical and biological applications. For example, a bicarbonate buffer helps maintain the pH of blood, a buffered saline maintains cellular contents at a consistent pH level, and buffers help maintain a narrow pH range for enzymes to function correctly.
Most biological samples used in research are kept at a pH of about 7.4. Common biological buffers used for tissue culture include Dulbeccos phosphate buffered saline (PBS), Tris base, HEPES, MOPS, PIPES, and other formulations developed for specific cell lines or applications. Most buffers used in cell culture are also DNase-, RNase-, and protease-free.
The choice of buffer for a particular biological reaction or site depends on several factors:
- Temperature
- Desired or target pH
- Buffer toxicity (to the system)
- Buffer interactions with other system components
FUJIFILM BIOSCIENCES INC RPMI Medium 1640 1X HEPES
RPMI Medium 1640 1X HEPES w/oL-Glutamine is designed for growth of humanLeukemia cells in either monolayers or suspension and is useful for a wide variety of suspension and monolayer cultures as well as for hybridomas
Hampton Research StockOptions CHES Buffer Kit, pH 8.6 to 10.0 in 0.1 pH unit increments
StockOptions CHES Buffer Kit, pH 8.6 to 10.0 in 0.1 pH unit increments
Alkali Scientific CellPro™ PBS, 10X, pH 7.4, 500mLCellPro™ PBS, 10X, pH 7.4, 500mL
Phosphate-buffered saline (PBS) is a balanced salt solution that is used for a variety of cell culture applications, such as washing cells before dissociation, transporting cells or tissue samples, diluting cells for counting, and preparing reagents.PBS is formulated without calcium and magnesium for rinsing chelators from the culture before cell dissociation. Concentrated forms of Gibco™ PBS may require pH adjustment at the time of dilution.
Cell Signaling Technology Tris-Glycine SDS Running Buffer (10X) 1000 ml
Tris-Glycine SDS Running Buffer (10X) 1000 ml
Sigma Aldrich Fine Chemicals Biosciences HEPES potassium salt | 82207-62-3 | MFCD00043438 | 25 g
HEPES potassium salt | Purity: ≥99.5% | Mol Wt: 276.39 | 82207-62-3 | MFCD00043438 | 25 g
ILEX LIFE SCIENCES LLC GELATIN SOLUTION 2.0 100 ML
PAN-Biotech Gelatin Solution 2% in DPBS, w/o: Ca, Mg (100 ml). DESCRIPTION: PAN-Biotech Gelatin Solution 2% in DPBS, w/o: Ca, Mg for laboratory research applications. Gelatin is a heterogenous mixture of water soluble proteins of high average molecular weight present in collagen. The proteins are extracted by boiling skin, tendons, ligaments, or bones in water. APPLICATION: Applications using gelatin include coating cell culture plates to improve cell attachment for a variety of cell types, addition to PCR to stabilize Taq DNA Polymerase, and use as a blocking agent in Western Blotting, ELISA, and immunohistochemistry. In bacteriology, gelatin can be used as a component of culture media. STORAGE: +2-8°C. MANUFACTURER: PAN-Biotech GmbH. COUNTRY OF ORIGIN: Germany.
Research Products International Corp PBS, 1X Solution, 1.0ml Pre-Filled Tubes, S, 1000 CS
PBS (Phosphate-Buffered Saline) is a balanced salt solution that helps maintain the pH and osmolarity of experimental systems, making it ideal for various biological and biochemical experiments.
1X Solution refers to a diluted concentration of a particular solution. In the case of PBS, a 1X solution means that it is prepared by diluting the concentrated stock solution to a specific ratio, usually 1 part stock solution to 9 parts water or another suitable solvent. 1.0ml Pre-Filled Tubes are small tubes that come pre-filled with a specified volume of liquid, in this case, 1.0 milliliters of PBS. These tubes are convenient and sterile, ensuring the integrity and purity of the solution. They are commonly used in laboratory settings to provide a controlled and standardized amount of PBS for various applications.
Bioworld BSA (1%) IN PBS, DNASE AND RNA
BSA (1%) in PBS, DNase and RNase Free0.79% Sodium Chloride, 0.02% Potassium Chloride, 0.06% Sodium Phosphate, 0.02% Potassium Phosphate, 1.0% BSA, DNAse/RNAse-Free
Revvity Health Sciences Inc DELFIA L*R wash concentrate (25x) Standard, 250 mL
DELFIA L*R wash concentrate (25x) Standard, 250 mL
Strem, An Ascensus Company CAS# 60-00-4. 500g. Ethylenediaminetetraacetic acid, 99+%. MFCD00003541
CAS# 60-00-4. 500g. Ethylenediaminetetraacetic acid, 99+%. MFCD00003541. Molecular Weight: 292.24. Molecular Formula: (HO2CCH2)2NCH2CH2N(CH2CO2H)2. Color/form: white xtl. Strem# 07-0573. http://www.strem.com/catalog/v/07-0573/
Boston Bioproducts Inc Tris-Base (2 M, pH > 10.0) - 250ml
Tris-base is a widely used biological buffer in various research applications in biochemistry, molecular biology, and cell biology. The buffering capabilities of Tris-base are effective in the pH range of 7.0-9.0, making it suited for various biological applications. This buffer is widely used in protocols for DNA and RNA extraction, in situ hybridization, protein extraction from cells, in agarose gel electrophoresis, polyacrylamide gel electrophoresis and as a wash buffer in Western blotting.