Life Science Buffers
Balanced salt solutions and powders used for molecular, protein, nucleic acid, and cell biology applications including cell culture, electrophoresis, ELISA, and chromatography processes; available in a variety of formulations and grades.
Buffer solutions serve to keep pH (acidity or alkalinity) nearly constant in a variety of chemical and biological applications. For example, a bicarbonate buffer helps maintain the pH of blood, a buffered saline maintains cellular contents at a consistent pH level, and buffers help maintain a narrow pH range for enzymes to function correctly.
Most biological samples used in research are kept at a pH of about 7.4. Common biological buffers used for tissue culture include Dulbeccos phosphate buffered saline (PBS), Tris base, HEPES, MOPS, PIPES, and other formulations developed for specific cell lines or applications. Most buffers used in cell culture are also DNase-, RNase-, and protease-free.
The choice of buffer for a particular biological reaction or site depends on several factors:
- Temperature
- Desired or target pH
- Buffer toxicity (to the system)
- Buffer interactions with other system components
Bioworld EGTA 0.5M, pH 8.0, DNase and RNase Free, 50 mL
EGTA Buffer is of a 0.5M concentration and pH of 8.0 that is DNase and RNase free. EGTA Buffer is a metal chelating agent specific for calcium ions. EGTA Buffer is commonly used in tissue culture media and biological buffers.
![eMolecules tert-butyl 7-(hydroxymethyl)-5-oxa-2-azaspiro[3.4]octane-2-carboxylate | 1330764-06-1 | MFCD20230641 | 1g](https://assets.fishersci.com/TFS-Assets/CCG/product-images/502158274.JPG-250.jpg)
eMolecules tert-butyl 7-(hydroxymethyl)-5-oxa-2-azaspiro[3.4]octane-2-carboxylate | 1330764-06-1 | MFCD20230641 | 1g
Pharmablock | tert-butyl 7-(hydroxymethyl)-5-oxa-2-azaspiro[3.4]octane-2-carboxylate | 1g | 551091973 | PB90623 | | 1330764-06-1 | MFCD20230641 | 243.303 | C12H21NO4
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Medchemexpress LLC 2-Morpholinoethanesulphonic acid monohydrate | 145224-94-8 | 99.9% | 500 G
2-Morpholinoethanesulphonic acid monohydrate | 145224-94-8 | 99.9% | 500 G
Research Products International Corp Phosphate Buffered Saline, 10X Solution, 1 Liter
PBS 10X Solution is a concentrated phosphate-buffered saline widely used in cell biology, immunohistochemistry, and molecular biology. It contains phosphate ions that maintain physiological pH and ionic strength, creating a stable environment for cells, tissues, and biomolecules. The 10X concentration allows for easy dilution to 1X for various applications such as washing, resuspending, or preparing samples. The phosphate buffer system resists pH changes, ensuring experimental consistency. PBS 10X is a reliable, versatile reagent that supports reproducible results in biological and biochemical workflows.
![Research Products International Corp CAPS [3-(Cyclohexylamino)-1-propanesulphonic acid], 500 Grams](https://assets.fishersci.com/TFS-Assets/CCG/product-images/VN00006095-C30060-500.0.JPG-250.jpg)
Research Products International Corp CAPS [3-(Cyclohexylamino)-1-propanesulphonic acid], 500 Grams
pKa (25oC): 10.4. The working pH range in aqueous solution is 9.7-11.1
Research Products International Corp MES, Anhydrous, 250 Grams
- CAS Number: 4432-31-9
- Chemical Formula: C6H13NO4S
- Molecular Weight: 195.24 g/mol
Quality Biological Inc PBS (10X) pH 7.2 20L
PBS (phosphate buffered saline) is a balanced salt solution used for a variety biological and cell culture applications such as washing cells before dissociation transporting cells or tissue diluting cells for counting and preparing reagents.Endotoxin and Performance tested Manufactured in an ISO 90012015 certified facility.
![Research Products International Corp CAPS [3-(Cyclohexylamino)-1-propanesulphonic acid], 100 Grams](https://assets.fishersci.com/TFS-Assets/CCG/product-images/VN00006095-C30060-100.0.JPG-250.jpg)
Research Products International Corp CAPS [3-(Cyclohexylamino)-1-propanesulphonic acid], 100 Grams
pKa (25oC): 10.4. The working pH range in aqueous solution is 9.7-11.1
Research Products International Corp TRIS BUFFERED SALINE, 10X SOLUTION, 4 LITER
TBS 10X Solution (Tris Buffered Saline) is a concentrated buffered salt solution containing Tris and sodium chloride. It maintains pH stability and provides ionic strength for biological experiments. Tris acts as a buffering agent to keep pH consistent, while sodium chloride mimics physiological conditions. Dilute 10X solution with distilled water to prepare working concentrations (e.g., 1X). TBS is widely used in protein and nucleic acid blotting, immunohistochemistry, and molecular biology techniques, ensuring stable and reproducible results across diverse applications.
![Research Products International Corp MES Monohydrate [2-(N-Morpholino)-ethanesulfonic acid], 100 Grams](https://assets.fishersci.com/TFS-Assets/CCG/product-images/VN00006095-M22040-100.0.JPG-250.jpg)
Research Products International Corp MES Monohydrate [2-(N-Morpholino)-ethanesulfonic acid], 100 Grams
Buffer for use in plant tissue culture.
- pKa (20°C): 6.15
- pH range: 5.5 - 6.7
Medchemexpress LLC Tapso | 68399-81-5 | 98.0% | 100 G
TAPSO is a widely used biological buffer and pH stabilization reagent. It offers an effective pH range between 7 and 8, with pKa values typically falling between 7.5 and 9.0. This compound contains Tris groups and has been observed to exhibit reactive activity with zwitterionic glycine peptides. It is primarily intended for research applications.
- Effective pH range of 7-8.
- pKa values ranging from 7.5-9.0.
- Contains Tris groups.
- Shows reactive activity with zwitterionic glycine peptides.
- Suitable for various research applications.