Life Science Buffers
Balanced salt solutions and powders used for molecular, protein, nucleic acid, and cell biology applications including cell culture, electrophoresis, ELISA, and chromatography processes; available in a variety of formulations and grades.
Buffer solutions serve to keep pH (acidity or alkalinity) nearly constant in a variety of chemical and biological applications. For example, a bicarbonate buffer helps maintain the pH of blood, a buffered saline maintains cellular contents at a consistent pH level, and buffers help maintain a narrow pH range for enzymes to function correctly.
Most biological samples used in research are kept at a pH of about 7.4. Common biological buffers used for tissue culture include Dulbeccos phosphate buffered saline (PBS), Tris base, HEPES, MOPS, PIPES, and other formulations developed for specific cell lines or applications. Most buffers used in cell culture are also DNase-, RNase-, and protease-free.
The choice of buffer for a particular biological reaction or site depends on several factors:
- Temperature
- Desired or target pH
- Buffer toxicity (to the system)
- Buffer interactions with other system components
Biocare Medical, LLC Tris Buffer Saline (TBS) Plus, 10X
For laboratory use and procedures requiring a high quality Tris buffer saline. TBS is sodium azide and thimerosal free and provides superior pH stability. It can also be used for manual immunohistochemistry applications or on an automated staining system with the addition of surfactant.
Medchemexpress LLC Tris buffered saline | 10 TABLETS
Tris buffered saline is supplied as ready-to-dissolve tablets for preparing Tris/NaCl buffer solutions commonly used in molecular biology and protein applications. The tablet format simplifies buffer preparation and storage while delivering a stable pH environment for assays and electrophoresis.
- Ready-to-use tablet format for convenient buffer preparation.
- One tablet dissolves in 15 mL deionized water to yield 0.05 M Tris and 0.15 M sodium chloride.
- pH approximately 7.6 at 25 °C.
- White to off-white solid tablets for easy visual quality check.
- Suitable for DNA/RNA extraction, PCR, and protein electrophoresis.
- Compact packaging reduces storage space and eases transport.
Enzo Life Sciences Wash buffer concentrate, (100ml)
Formulation: Liquid. TRIS buffered saline containing detergents. Long Term Storage: Ambient. Applications: ELISA .
Indofine Chemical 1,3,5-Triphenyl Tetraz, 1 Gm
1,3,5-TRIPHENYL TETRAZOLIUM FORMAZAN, Products for Life Science, 531-52-2, 1 gm
Medchemexpress LLC Bes | 10191-18-1 | MFCD00007533 | 98.0% | 213.25 | 100 G
BES is a secondary standard biochemical buffer with an effective pH range of 6.4 to 7.8 (pKa: 7.15 at 20 °C). It is utilized in diagnostic testing and is intended for research use only.
- Effective pH range of 6.4 to 7.8
- Utilized in diagnostic testing applications
- Purity of 98.0%
- Molecular weight of 213.25
- Appearance as a white to off-white solid
Medchemexpress LLC 2-Morpholinoethanesulphonic acid hydrate | 1266615-59-1 | 99.9% | 50 G
MES hydrate is an amphoteric ion buffer with a buffering capacity ranging from pH 5.5-7.0. It is widely used in biochemistry and molecular biology experiments for applications such as cell culture, enzyme activity determination, electrophoresis, and protein studies.
- Provides effective buffering capacity at approximately pH 6.15.
- Its pKa value changes with temperature, necessitating consideration for different experimental conditions.
- Exhibits good buffering properties in biological experiments.
- Shows poor affinity with metal ions, allowing its use in studies involving them.
Medchemexpress LLC MOPS (sodium salt) | 71119-22-7 | 99.8% | 50 G
MOPS sodium salt is a buffering agent used in biological applications. It helps maintain the pH of mammalian cell culture media and significantly improves the thermal stability of BSA. This product is also suitable for the preparation of keratinocyte and microbial culture media.
- Maintains pH of mammalian cell culture media
- Enhances thermal stability of BSA
- Suitable for preparing keratinocyte culture medium
- Suitable for preparing microbial culture medium
- Can be used in co-culture of keratinocytes and microorganisms
Medchemexpress LLC 2-Morpholinoethanesulphonic acid monohydrate | 145224-94-8 | 99.95% | 250 MG
MES monohydrate is an analytical standard intended for research and analytical applications. It is an amphoteric ion buffer with a buffer capacity ranging from pH 5.5-7.0. As a Good's buffer, it is widely used in biochemistry and molecular biology experiments as a reference standard for assays.
- Used in cell culture
- Used in enzyme activity determination
- Used in electrophoresis
- Used in protein studies
- Commonly used in qualitative, quantitative, and methodological research experiments in HPLC, GC, and MS
Medchemexpress LLC MES hydrate | 1266615-59-1 | 99.9% | 5 G
MES (2-Morpholinoethanesulphonic acid) hydrate is an amphoteric ion buffer with a buffer capacity ranging from pH 5.5-7.0. As a Good's buffer, it is widely used in biochemistry and molecular biology experiments, including cell culture, enzyme activity determination, electrophoresis, and protein studies. It is a zwitterionic buffer that provides effective buffering capacity around pH 6.15. Its pKa value changes with temperature. MES hydrate exhibits poor affinity with metal ions (binding constants at 20 °C and 0.1 M: Mg2+, 0.8; Ca2+, 0.8; Mn2+, 1.7), making it suitable for studies involving metal ions.
- Appearance: solid
- Color: white to off-white
- Formula: C6H13NO4S.xH2O
- Shipping: room temperature in continental US; may vary elsewhere
- Storage: store at room temperature, keep dry and cool. In solvent: -80°C for 1 year, -20°C for 6 months
- Solubility: H2O : 175 mg/mL (requires ultrasonic)
Medchemexpress LLC Glycine, N-(2-amino-2-oxoethyl)-N-(carboxymethyl)-, sodium salt (1:2) | 41689-31-0 | 98.0% | 50 G
ADA disodium is a buffer salt derived from adenosine deaminase, commonly used in biochemical research for preparing enzyme buffers. This product is intended for research use only and is not for human or animal therapeutic use.
- Solid appearance
- White to off-white color
- Molecular weight: 234.12
- Chemical formula: C6H8N2Na2O5
- Solubility in water: 350 mg/mL
- Store at 4°C, sealed, away from moisture
- Store in solvent at -80°C for 6 months or -20°C for 1 month, sealed, away from moisture