Life Science Buffers
Balanced salt solutions and powders used for molecular, protein, nucleic acid, and cell biology applications including cell culture, electrophoresis, ELISA, and chromatography processes; available in a variety of formulations and grades.
Buffer solutions serve to keep pH (acidity or alkalinity) nearly constant in a variety of chemical and biological applications. For example, a bicarbonate buffer helps maintain the pH of blood, a buffered saline maintains cellular contents at a consistent pH level, and buffers help maintain a narrow pH range for enzymes to function correctly.
Most biological samples used in research are kept at a pH of about 7.4. Common biological buffers used for tissue culture include Dulbeccos phosphate buffered saline (PBS), Tris base, HEPES, MOPS, PIPES, and other formulations developed for specific cell lines or applications. Most buffers used in cell culture are also DNase-, RNase-, and protease-free.
The choice of buffer for a particular biological reaction or site depends on several factors:
- Temperature
- Desired or target pH
- Buffer toxicity (to the system)
- Buffer interactions with other system components
eMolecules 502650-56-8 | methyl cis-3-(hydroxymethyl)cyclohexanecarboxylate | 1g
Pharmablock - Stock | methyl cis-3-(hydroxymethyl)cyclohexanecarboxylate | 1g | 534153256 | PBJL037 | 502650-56-8 | 172.224 | C9H16O3
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Thermo Scientific Production Chemicals and Services 50 MM TRIS-HCL 10 MM EDTA 5ML
NC3588252 50 MM TRIS-HCL 10 MM EDTA 5ML
Thermo Scientific Production Chemicals and Services 50 MM TRIS-HCL 10 MM EDTAPH8
NC3588273 50 MM TRIS-HCL 10 MM EDTAPH8
Biotang Inc TRIS-HCL Buffer 1.0M, pH 8.2 (0.2 micron filtered), 500 ml
TRIS-HCL Buffer 1.0M, pH 8.2 (0.2 micron filtered), 500 ml.
Biotang Inc HEPES Lysis Buffer with Triton (2X), 125 ml
HEPES Lysis Buffer with Triton (2X), 125 ml.
Alkali Scientific TRIS CAPS Buffer 10X Solution, 2 x 500 Milliliter Bottles
TRIS CAPS Buffer 10X Solution is designed for use in capillary electrophoresis and other advanced electrophoretic techniques. This formulation, available in two 500mL bottles, ensures convenient preparation and consistent results during capillary separation of charged biomolecules. The 10X solution can be diluted to create the working concentration needed for various electrophoresis applications. TRIS CAPS buffer is particularly useful in separating proteins and nucleic acids in a high-resolution format. This buffer is often used in protein and DNA/RNA electrophoresis protocols, providing stable pH conditions that enhance separation and improve sensitivity during detection.
![Alkali Scientific TBE 10X Solution [TRIS borate EDTA 10X Solution], 4 L](https://assets.fishersci.com/TFS-Assets/CCG/product-images/5000660682.jpg-250.jpg)
Alkali Scientific TBE 10X Solution [TRIS borate EDTA 10X Solution], 4 L
TBE 10X Solution is an essential buffer used in the electrophoresis of nucleic acids such as DNA and RNA. This 4-liter bottle contains a concentrated mixture of Tris, borate, and EDTA, which provides the necessary pH and ionic environment for the proper migration of nucleic acid samples. TBE is particularly effective for separating small DNA fragments and is widely used in both research and clinical laboratories. By diluting the 10X stock to 1X, laboratories can prepare a buffer suitable for use in standard electrophoresis procedures. It is an important reagent for molecular biology applications requiring high-purity separation of nucleic acids.
eMolecules 1031442-66-6 | 4-BENZYL-2-(HYDROXYMETHYL)HOMOMORPHOLINE | AstaTech | MFCD14585013 | 221.300 | C13H19NO2 | 95.000 | OCC1CN(Cc2ccccc2)CCCO1 | 0.25g | 256638850
4-BENZYL-2-(HYDROXYMETHYL)HOMOMORPHOLINE | AstaTech | 1031442-66-6 | MFCD14585013 | 221.300 | C13H19NO2 | 95.000 | OCC1CN(Cc2ccccc2)CCCO1 | 0.25g | 256638850
Crescent Chemical Co Inc TRIS BUFFER PH 7.5 1 M SOLN
Tris buffer pH 7.5, 1 M solution molecular biology grade DNase, RNase, Protease not detected. The pH value of Tris buffer is temperature and concentration dependent. For Tris buffers, pH increases about 0.03 unit per degree C decrease in temperature, and decreases 0.03 - 0.05 unit