Life Science Buffers
Balanced salt solutions and powders used for molecular, protein, nucleic acid, and cell biology applications including cell culture, electrophoresis, ELISA, and chromatography processes; available in a variety of formulations and grades.
Buffer solutions serve to keep pH (acidity or alkalinity) nearly constant in a variety of chemical and biological applications. For example, a bicarbonate buffer helps maintain the pH of blood, a buffered saline maintains cellular contents at a consistent pH level, and buffers help maintain a narrow pH range for enzymes to function correctly.
Most biological samples used in research are kept at a pH of about 7.4. Common biological buffers used for tissue culture include Dulbeccos phosphate buffered saline (PBS), Tris base, HEPES, MOPS, PIPES, and other formulations developed for specific cell lines or applications. Most buffers used in cell culture are also DNase-, RNase-, and protease-free.
The choice of buffer for a particular biological reaction or site depends on several factors:
- Temperature
- Desired or target pH
- Buffer toxicity (to the system)
- Buffer interactions with other system components
Medchemexpress LLC Hepes hemisodium salt | 103404-87-1 | MFCD00036463 | 99.0% | 249.80 g/mol | C8H17N2O4S · 1/2 Na | 500 MG
HEPES hemisodium salt is the hemisodium salt form of HEPES, a zwitterionic buffering agent commonly used to maintain physiological pH in biological and biochemical research. It delivers reliable buffer capacity near pH 6.8-8.2 and is supplied as a solid reagent for preparation of aqueous buffers for cell culture, enzymatic assays, and protein work.
- Provides stable buffering at pH 6.8-8.2
- Low reactivity with biological systems
- High assay purity suitable for analytical applications
- Easy to dissolve for reproducible buffer preparation
- Available in convenient lab-scale pack sizes
Alkali Scientific QuickSilver™ Buffer Powder, Tris-Glycine-SDS Buffer Powder, 50 pouches
QuickSilverª Buffer Powder Packs offer extreme convenience and will change the way you prepare buffers in your lab. They are quick, easy to use, and come in a variety of buffers for Nucleic Acid and Protein Electrophoresis, Western Blotting, ELISA, and Cell Culture. Quick and Economical (and low shipping costs!) Convenient premeasured pouches Each pouch makes 1L of 1X buffer No pH adjustment needed Nuclease and protease free Quality and consistency guaranteed Long shelf life Buffers for Electrophoresis of Nucleic Acids and Proteins: TBE, TAE, Fast Running Buffer, Tris-Glycine-SDS, Tris-MOPS-SDS, Tris-MES-SDS Buffers for Western Blotting, ELISA and Cell Culture: TBS, TBST, PBS, PBST
Pfanstiehl Inc Tris Base (Tromethamine) High Purity, Low Endotoxin, Low Metals, USP, EP, BP, ChP (T-153) 1 Kg GMP, Multi-compendial CAS: 77-86-1 Injectable Excipient Grade
Pfanstiehl Tris Base, High Purity, Low Endotoxin, Low Metals, USP, EP, BP, ChP is a multi-compendial buffer component that has been specifically designed and formulated to remove impurities such as endotoxin, trace metals, and nitrosamines. Manufactured in the USA under ICH-Q7 cGMP conditions, Pfanstiehl Tris Base meets parenteral (injectable) excipient grade standards, making it ideal for pharmaceutical, biologic, and vaccine bioprocessing and formulation. Pfanstiehl Brand Tris Base is compliant with USP (United States Pharmacopoeia) EP (European Pharmacopoeia), BP (British Pharmacopeia) and ChP (Chinese Pharmacopeia) Compendial, U.S.P. , cGMP , pharmaceutical grade .
Crescent Chemical Co Inc TAE BUFFER (10X) MOL BIO GRADE
TAE buffer (10x) molecular biology grade 10 x concentrated aqueous solution.TAE Buffer is used for the electrophoresis of nucleic acids. TAE has a lower buffer capacity than TBE, however linear ds DNA tends to run faster in TAE than in TBE. Size - 1L Stor
Crescent Chemical Co Inc HYDROXYETHYL)PIPERAZINE-N'-2-E
Hydroxyethyl)piperazine-N'-2-ethane sulfonic acid -Na-salt analytical grade pKa 20 = 7.55. Buffering substance for biochemistry and molecular biology. Size - 1KG Storage Conditions - +15 °C TO +30 °C Catalog Number - 25249.04 CAS 75277-39-3
Crescent Chemical Co Inc SERVA TRIS-TRICINE/SDS BUFFER
SERVA Tris-Tricine/SDS electrophoresis buffer (10x) Running buffer for SDS PAGE. Supplied as 10 x concentrate. Contains 1 M Tris, 1 M Tricine and 1 % SDS. Size - 1L Storage Conditions - +15 °C TO +30 °C Catalog Number - 42552.01
Crescent Chemical Co Inc SERVA TRIS-TRICINE/SDS BUFFER
SERVA Tris-Tricine/SDS electrophoresis buffer (20x) Running buffer for SDS PAGE. Optimized formulation to improve sharpness of bands, especially when used together with SERVAGel Neutral pH 7.4 (cat. no. 43220 and 43221) precast gels. Supplied as 20 x concentrate. Contains 1.2 M Tris (pH 8.5), 0.8
Alkali Scientific CellPro™ PBS, 10X, pH 7.4, 500mL, 500ml, 10/case
CellPro™ PBS, 10X, pH7.4, 500mL Phosphate-buffered saline (PBS) is a balanced salt solution that is used for a variety of cell culture applications, such as washing cells before dissociation, transporting cells or tissue samples, diluting cells for counting, and preparing reagents. PBS is formulated without calcium and magnesium for rinsing chelators from the culture before cell dissociation. Concentrated forms of Gibco™ PBS may require pH adjustment at the time of dilution.