Life Science Buffers
Balanced salt solutions and powders used for molecular, protein, nucleic acid, and cell biology applications including cell culture, electrophoresis, ELISA, and chromatography processes; available in a variety of formulations and grades.
Buffer solutions serve to keep pH (acidity or alkalinity) nearly constant in a variety of chemical and biological applications. For example, a bicarbonate buffer helps maintain the pH of blood, a buffered saline maintains cellular contents at a consistent pH level, and buffers help maintain a narrow pH range for enzymes to function correctly.
Most biological samples used in research are kept at a pH of about 7.4. Common biological buffers used for tissue culture include Dulbeccos phosphate buffered saline (PBS), Tris base, HEPES, MOPS, PIPES, and other formulations developed for specific cell lines or applications. Most buffers used in cell culture are also DNase-, RNase-, and protease-free.
The choice of buffer for a particular biological reaction or site depends on several factors:
- Temperature
- Desired or target pH
- Buffer toxicity (to the system)
- Buffer interactions with other system components
Medchemexpress LLC HEPPS | 16052-06-5 | 99.0% | 25 G
HEPPS (EPPS) is a buffering agent with a useful pH range from 7.3 to 8.7. It reduces Aβ-aggregate-induced memory deficits and rescues cognitive deficits in mice. EPPS is orally active and penetrates the blood-brain barrier.
- Buffering agent with a useful pH range from 7.3 to 8.7.
- Reduces Aβ-aggregate-induced memory deficits.
- Rescues cognitive deficits in mice.
- Orally active.
- Penetrates the blood-brain barrier.
Sigma Aldrich Fine Chemicals Biosciences PIPES BioPerformance Certi1KG
PIPES BioPerformance Certi1KG
Boston Bioproducts Inc HEPES Buffer (1 M, pH 7.6, pH Adjusted with KOH) - 500ml
HEPES Buffer (1 M pH 7 6 pH Adjusted with KOH) - 500ml (For Research Use and Further Manufacturing Only)
![Alkali Scientific HEPES, Free Acid [N (2 Hydroxyethyl) piperazine N' (2 ethanesulfonic acid)], 25 Kg](https://assets.fishersci.com/TFS-Assets/CCG/product-images/5000660290.jpg-250.jpg)
Alkali Scientific HEPES, Free Acid [N (2 Hydroxyethyl) piperazine N' (2 ethanesulfonic acid)], 25 Kg
HEPES, Free Acid is a widely used biochemical reagent, available in a 25kg package for larger-scale applications. This highly stable buffer is effective in maintaining a pH range of 6.8–8.2, making it ideal for a variety of biochemical and biological applications such as protein analysis, cell culture, and drug formulation. HEPES helps maintain physiological pH levels, preventing changes that could interfere with experiments. This bulk package is especially beneficial for laboratories requiring frequent buffer preparation, allowing for efficient cost-saving and consistent results across a wide range of biological and chemical protocols.
Genscript Corporation MES SDS Running Buffer Powder
MES SDS Running Buffer is formulated for running ExpressPlusᵀᴹ and SurePAGEᵀᴹ, Bis-Tris gels. This buffer is ideal for separating small- and medium- sized proteins. For separation of large- to medium- sized proteins, Tris-MOPS SDS buffer (M00138) is recommended.
Reconstitute with 1000 ml deionized water to make 1X running buffer per pack of powder. Do not use acid or base to adjust the pH.
Sigma Aldrich Fine Chemicals Biosciences HEPES buffer solution 1 M in H2O | 7365-45-9 | MFCD00006158 | 500ML
HEPES buffer solution 1 M in H2O | 7365-45-9 | MFCD00006158 | 500ML
Apexbio Technology LLC MES sodium salt 71119-23-8 1g
MES sodium salt (CAS 71119-23-8) is a buffering agent designed to maintain stable pH conditions in the range of 5 5 7 0 supporting physiological experimental environments MES sodium salt does not have a direct cellular or molecular target and is not a small-molecule inhibitor agonist or antagonist Its key role is pH stabilization in various biological and biochemical assays including enzymatic activity assays receptor pathway investigations and isolation of cellular organelles or biomolecules Experimental models include plant cell culture systems and physiological in vitro assays Based on these properties MES sodium salt holds research potential in applications such as plant cell culture controlled metabolic pathway studies and signal transduction research requiring consistent pH conditions
Research Products International Corp MES, Anhydrous, 250 Grams
- CAS Number: 4432-31-9
- Chemical Formula: C6H13NO4S
- Molecular Weight: 195.24 g/mol
Sigma Aldrich Fine Chemicals Biosciences PIPES Dipotassium Salt | 108321-27-3 | MFCD00069751 | 100g
PIPES Dipotassium Salt | Purity: 99% | Mol Wt: 378.55 | 108321-27-3 | MFCD00069751 | 100g
Sigma Aldrich Fine Chemicals Biosciences CAPS | 1135-40-6 | MFCD00003837 | 1kg
CAPS | Purity: 99% | MW: 221.32 | 1135-40-6 | MFCD00003837 | 1kg
Biotang Inc HEPES-Buffer, 0.5M pH 7.6, 100ml 500 mM HEPES (4-(2-Hydroxyethyl)piperazine-1-ethanesulfonic acid), pH 7.6 +/- 0.15. BB-2078
HEPES-Buffer, 0.5M pH 7.6, 100ml 500 mM HEPES (4-(2-Hydroxyethyl)piperazine-1-ethanesulfonic acid), pH 7.6 +/- 0.15. BB-2078.
Sigma Aldrich Fine Chemicals Biosciences MES monohydrate BioUltra, for molecular biology, >=99.5% (T) | 145224-94-8 | MFCD00149409 | 50G
MES monohydrate BioUltra, for molecular biology, >=99.5% (T) | Purity: >=99.5% (T) | Mol Wt: 213.25 | 145224-94-8 | MFCD00149409 | 50G
![Alkali Scientific MES Sodium Salt [2 (N Morpholino)ethanesulfonic acid], sodium salt, 100 G](https://assets.fishersci.com/TFS-Assets/CCG/product-images/5000660293.jpg-250.jpg)
Alkali Scientific MES Sodium Salt [2 (N Morpholino)ethanesulfonic acid], sodium salt, 100 G
MES Sodium Salt (2-(N-Morpholino) ethanesulfonic acid), sodium salt, in a 100g volume is a biochemical reagent designed for pH buffering in laboratory experiments. It is commonly used in biological assays, cell culture, and protein purification due to its ability to maintain a stable and non-interfering pH. The sodium salt form of MES is particularly useful in experiments where higher ionic strength is required. This 100g size is ideal for smaller experiments and research studies that need precise pH control without requiring large quantities of reagent. MES Sodium Salt is an essential component in molecular biology and biochemistry for maintaining optimal conditions in sensitive biological assays.
eMolecules 29915-38-6 | TAPS | Ambeed | MFCD00007538 | 243.270 | C7H17NO6S | 98.000 | OCC(CO)(CO)NCCCS(O)(=O)=O | 5g | 600837773
TAPS | Ambeed | 29915-38-6 | MFCD00007538 | 243.270 | C7H17NO6S | 98.000 | OCC(CO)(CO)NCCCS(O)(=O)=O | 5g | 600837773
Rockland Immunochemicals 1.0 M HEPES pH 7.2 - MB-062-0100
HEPES is a useful buffer for pH's ranging from 6.8-8.2. This makes HEPES ideal for cell culturing because of its usefulness in maintaining pH changes in presence of carbon dioxide production from cellular respiration.1.0 M HEPES pH 7.2 is ideal for investigators in Molecular Biology, Cell Biology, and Immunology researchers.