Life Science Buffers
Balanced salt solutions and powders used for molecular, protein, nucleic acid, and cell biology applications including cell culture, electrophoresis, ELISA, and chromatography processes; available in a variety of formulations and grades.
Buffer solutions serve to keep pH (acidity or alkalinity) nearly constant in a variety of chemical and biological applications. For example, a bicarbonate buffer helps maintain the pH of blood, a buffered saline maintains cellular contents at a consistent pH level, and buffers help maintain a narrow pH range for enzymes to function correctly.
Most biological samples used in research are kept at a pH of about 7.4. Common biological buffers used for tissue culture include Dulbeccos phosphate buffered saline (PBS), Tris base, HEPES, MOPS, PIPES, and other formulations developed for specific cell lines or applications. Most buffers used in cell culture are also DNase-, RNase-, and protease-free.
The choice of buffer for a particular biological reaction or site depends on several factors:
- Temperature
- Desired or target pH
- Buffer toxicity (to the system)
- Buffer interactions with other system components
LAB BIOREAGENTS LowBinding I Buffer 500ml
Lab Bioreagents LowBindings (I, II, III, IV, V, VI, VII) are newly developed assay buffers that provides exceptional performance and broad compatibility for Western blot, ELISA, IHC and nucleic acid detection assays. The LowBindings helps to reduce the non-specific bindings, negative effects of interfering molecules, and cross-reactivities of antibodies and proteins from biomatrix. Therefore, LowBindings exhibit the low matrix interference and high signal-to-noise, by which the assay sensitivity and specificity have been significantly improved. As many factors are involved in immunoassays, there is no single assay buffer that is ideal for each assay. LowBindings (I, II, III, IV, V, VI, VII) are a variety of buffers based on different formulations.
![Alkali Scientific MOPS [3 (N morpholino) propanesulfonic acid], 25 G](https://assets.fishersci.com/TFS-Assets/CCG/product-images/5000660020.jpg-250.jpg)
Alkali Scientific MOPS [3 (N morpholino) propanesulfonic acid], 25 G
MOPS (3-(N-morpholino)propane-1-sulfonic acid) is a high-quality biochemical reagent used for pH buffering in laboratory research. This 25g package of MOPS is ideal for small-scale laboratory applications that require a stable pH range of 6.5 to 7.9. MOPS is widely used in molecular biology, protein chemistry, and biochemical assays where maintaining precise pH conditions is essential. It is commonly used in enzyme studies, electrophoresis, and protein purification processes. The small packaging size is suitable for labs with lower buffer requirements or for preparing small quantities of buffer solutions for specific experiments.
eMolecules Ambeed / 2-(Bis(2-hydroxyethyl)amino)acetic acid / 10g / 525008933 / A114701 / / 150-25-4 / MFCD00004295 / 163.173 / C6H13NO4
Ambeed / 2-(Bis(2-hydroxyethyl)amino)acetic acid / 10g / 525008933 / A114701 / / 150-25-4 / MFCD00004295 / 163.173 / C6H13NO4
Gold Biotechnology Inc MES Free Acid 100 g
MES is a buffering agent used in biochemistry and molecular biology that was selected and described by Good et al It is a zwitterionic morpholinic buffer that is useful for a pH range of 5 5 6 7 and commonly used for cell culture media as a running buffer in electrophoresis and for protein purification in chromatography MES lacks the ability to form a complex with most metal ions and is recommended for use as a non-coordinating buffer in solutions with metal ions MES is often used in buffered culture media for bacteria yeast and mammalian cells It is toxic towards plants at high concentrations but can be used in culture media at lower concentrations for plants Due to low ionic mobility MES is regarded as an excellent buffer for use in capillary electrochromatography MES is often employed as a substitute for the highly toxic cacodylate as well as a substitute for the non-zwitterionic buffers citrate and malate
Medchemexpress LLC 2-Morpholinoethanesulphonic acid monohydrate | 145224-94-8 | 99.95% | 250 MG
MES monohydrate is an analytical standard intended for research and analytical applications. It is an amphoteric ion buffer with a buffer capacity ranging from pH 5.5-7.0. As a Good's buffer, it is widely used in biochemistry and molecular biology experiments as a reference standard for assays.
- Used in cell culture
- Used in enzyme activity determination
- Used in electrophoresis
- Used in protein studies
- Commonly used in qualitative, quantitative, and methodological research experiments in HPLC, GC, and MS
Crescent Chemical Co Inc TAE BUFFER (10X) MOL BIO GRADE
TAE buffer (10x) molecular biology grade 10 x concentrated aqueous solution.TAE Buffer is used for the electrophoresis of nucleic acids. TAE has a lower buffer capacity than TBE, however linear ds DNA tends to run faster in TAE than in TBE. Size - 1L Stor
American Research Products Inc TRIS-ACETATE BUFFER, 0.2M,
TRIS-Acetate Buffer, 0.2M, pH 7.8-500ml. 0.2M Tris base dissolved in 18.2 megohms water, pH adjusted to 7.8 with glacial acetic acid and filtered through 0.22-micron filter.
Biotang Inc Tris-Buffered Saline 10X pH 7.6 (BM-615): 250 mM Tris, 27 mM KCl, 1.37 M NaCl, pH 7.6 +/- 0.2. Prepared in 18.2 megohms water. To make 1X, dilute 10-fold with deionized water. The pH of the 1X should be 7.6 +/- 0.2.
Tris-Buffered Saline 10X pH 7.6 (BM-615): 250 mM Tris, 27 mM KCl, 1.37 M NaCl, pH 7.6 +/- 0.2. Prepared in 18.2 megohms water. To make 1X, dilute 10-fold with deionized water. The pH of the 1X should be 7.6 +/- 0.2.
![Alkali Scientific DIPSO [3 (N,N Bis(2 Hydroxyethyl)amino) 2 hydroxypropane sulfonic acid], 25 G](https://assets.fishersci.com/TFS-Assets/CCG/product-images/5000660391.jpg-250.jpg)
Alkali Scientific DIPSO [3 (N,N Bis(2 Hydroxyethyl)amino) 2 hydroxypropane sulfonic acid], 25 G
DIPSO (3 [N,N-Bis(2 Hydroxyethyl)amino] 2-hydroxypropane sulfonic acid) is a biochemical reagent available in a 25g bottle. This buffering agent is widely used in molecular biology, biochemistry, and analytical chemistry for maintaining stable pH levels in low pH environments. DIPSO is ideal for enzyme assays, protein research, and chromatography, where maintaining a precise pH is critical. It is also useful in protein purification and electrophoresis, providing consistent and reliable results. The 25g size is perfect for smaller scale experiments or routine laboratory applications where a high-quality buffering solution is necessary for pH regulation.
![Alkali Scientific PIPES Sodium Salt [Piperazine N,N' bis(2 ethanesulfonic acid) sodium salt], 500 Grams](https://assets.fishersci.com/TFS-Assets/CCG/product-images/5000659887.jpg-250.jpg)
Alkali Scientific PIPES Sodium Salt [Piperazine N,N' bis(2 ethanesulfonic acid) sodium salt], 500 Grams
PIPES Sodium Salt (Piperazine N,N' bis(2 ethanesulfonic acid) sodium salt) is a biochemical reagent used to create buffer solutions that maintain a stable pH environment. This 500g quantity is ideal for large-scale laboratory applications, such as enzyme reactions, protein chemistry, and molecular biology experiments. PIPES sodium salt buffers maintain a stable pH around 7.0, which is essential for preserving enzyme activity and protein integrity in biological assays. The high buffering capacity of PIPES sodium salt ensures pH consistency, making it a popular choice in cellular studies, microbiology, and biochemical research involving delicate biochemical reactions.