Life Science Buffers
Balanced salt solutions and powders used for molecular, protein, nucleic acid, and cell biology applications including cell culture, electrophoresis, ELISA, and chromatography processes; available in a variety of formulations and grades.
Buffer solutions serve to keep pH (acidity or alkalinity) nearly constant in a variety of chemical and biological applications. For example, a bicarbonate buffer helps maintain the pH of blood, a buffered saline maintains cellular contents at a consistent pH level, and buffers help maintain a narrow pH range for enzymes to function correctly.
Most biological samples used in research are kept at a pH of about 7.4. Common biological buffers used for tissue culture include Dulbeccos phosphate buffered saline (PBS), Tris base, HEPES, MOPS, PIPES, and other formulations developed for specific cell lines or applications. Most buffers used in cell culture are also DNase-, RNase-, and protease-free.
The choice of buffer for a particular biological reaction or site depends on several factors:
- Temperature
- Desired or target pH
- Buffer toxicity (to the system)
- Buffer interactions with other system components
Sigma Aldrich Fine Chemicals Biosciences Dulbeccos Modified Eagles500ML
This DMEM-Low glucose medium is a 1x complete medium with sodium pyruvate added. It also differs from the original DMEM-Hi formulation wherein pyridoxine is substituted for pyridoxal. Pyridoxal is an unstable component of media.
Sigma Aldrich Fine Chemicals Biosciences Dulbeccos Phosphate Bufer500ML
Dulbecco Phosphate Buffered Saline (DPBS) is a balanced salt SOLUTIONon.
Promocell GmbH PromoCell Hepes BSS (30 mM) 250 ml, HEPES Buffered Saline Solution
Buffers and Salt Solutions are frequently used in tissue culture, cell isolation procedures, and molecular biology.For convenience, PromoCell offers ready-to-use Buffers and Salt Solutions. They are prepared from raw materials of the highest quality using tissue culture grade water.HEPES Buffered Saline Solution (30 mM).
Sigma Aldrich Fine Chemicals Biosciences PIPES BioPerformance Certi1KG
PIPES BioPerformance Certi1KG
Sigma Aldrich Fine Chemicals Biosciences Tricine BioPerformance Certified, suitable for cell culture, >=99% (titration) | 5704-04-1 | MFCD00004277 | 25G
Tricine BioPerformance Certified, suitable for cell culture, >=99% (titration) | Purity: >=99% (titration) | Mol Wt: 179.17 | 5704-04-1 | MFCD00004277 | 25G
Sigma Aldrich Fine Chemicals Biosciences HEPES | 7365-45-9 | MFCD00006158 | 25g
HEPES | Purity: 99.5% | Mol Wt: 238.3 | 7365-45-9 | MFCD00006158 | 25g
Sigma Aldrich Fine Chemicals Biosciences HEPES BioPerformance Certified | 7365-45-9 | MFCD00006158 | 25g
HEPES BioPerformance Certified | 99.5% | 238.3 | 7365-45-9 | MFCD00006158 | 25g
Sigma Aldrich Fine Chemicals Biosciences HEPES solution | 7365-45-9 | MFCD00006158 | 100 mL
HEPES solution | Mol Wt: 238.31 | 7365-45-9 | MFCD00006158 | 100 mL
Sigma Aldrich Fine Chemicals Biosciences PIPES | 5625-37-6 | MFCD00006159 | 500g
PIPES | Purity: 99% | Mol Wt: 302.37 | 5625-37-6 | MFCD00006159 | 500g
Sigma Aldrich Fine Chemicals Biosciences BIS-TRIS BioPerformance Certified, suitable for cell culture, suitable for insect cell culture, >=98.0% | 6976-37-0 | MFCD00002853 | 1KG
BIS-TRIS BioPerformance Certified, suitable for cell culture, suitable for insect cell culture, >=98.0% | Purity: >=98.0% | Mol Wt: 209.24 | 6976-37-0 | MFCD00002853 | 1KG
Sigma Aldrich Fine Chemicals Biosciences BIS-TRIS >=98.0% (titration) | 6976-37-0 | MFCD00002853 | 25G
BIS-TRIS >=98.0% (titration) | Purity: >=98.0% (titration) | Mol Wt: 209.24 | 6976-37-0 | MFCD00002853 | 25G
Sigma Aldrich Fine Chemicals Biosciences Ethylenediaminetetraacetic Acid | 60-00-4 | MFCD00003541 | 100g
Ethylenediaminetetraacetic Acid | Purity: 98% | Mol Wt: 292.24 | 60-00-4 | MFCD00003541 | 100g
Promocell GmbH PromoCell Hepes BSS (30 mM) 30 ml, HEPES Buffered Saline Solution
Buffers and Salt Solutions are frequently used in tissue culture, cell isolation procedures, and molecular biology.For convenience, PromoCell offers ready-to-use Buffers and Salt Solutions. They are prepared from raw materials of the highest quality using tissue culture grade water.HEPES Buffered Saline Solution (30 mM).
Sigma Aldrich Fine Chemicals Biosciences Dulbeccos Modified Eagles500ML
This DMEM-Hi glucose medium is a 1x complete medium with no added factors (common modifications) such as L-alanyl-L-glutamine HEPES or sodium pyruvate. It differs from the original DMEM-Hi formulation wherein pyridoxine is substituted for pyridoxal. Pyridoxal is an unstable component of media. This medium requires supplementation with L-glutamine or L-alanyl-L-glutamine.