Life Science Buffers
Balanced salt solutions and powders used for molecular, protein, nucleic acid, and cell biology applications including cell culture, electrophoresis, ELISA, and chromatography processes; available in a variety of formulations and grades.
Buffer solutions serve to keep pH (acidity or alkalinity) nearly constant in a variety of chemical and biological applications. For example, a bicarbonate buffer helps maintain the pH of blood, a buffered saline maintains cellular contents at a consistent pH level, and buffers help maintain a narrow pH range for enzymes to function correctly.
Most biological samples used in research are kept at a pH of about 7.4. Common biological buffers used for tissue culture include Dulbeccos phosphate buffered saline (PBS), Tris base, HEPES, MOPS, PIPES, and other formulations developed for specific cell lines or applications. Most buffers used in cell culture are also DNase-, RNase-, and protease-free.
The choice of buffer for a particular biological reaction or site depends on several factors:
- Temperature
- Desired or target pH
- Buffer toxicity (to the system)
- Buffer interactions with other system components
Dojindo Molecular Technologies Inc HEPES (100 G), Good's buffer, CAS: 7365-45-9, Purity: ≥99.0% (Titration), pKa=7.55, Active pH 6.8-8.2, made in Japan
Buffers perform an important task in biological experiments, often designed to keep pH within the 6-8 range where most biological reactions occur. Dr. Norman Good, the developer of HEPES, wanted to create buffers that prioritized this function, while also providing other benefits. Dr. Good produced a series of “Good buffers” – including HEPES (2-[4-(2-Hydroxyethyl)-1-piperazinyl]ethanesulfonic acid) – that are nearly ubiquitous in their usage even now. The Good series of buffers share the following additional characteristics: 1) High water-solubility. 2) Low cell membrane permeability. 3) Consistent acid-base dissociation constants. 4) Low metal-chelating capability. 5) High chemical stability. 6)Low absorption spectra in UV and visible regions.
Dojindo Molecular Technologies Inc TAPS (25G), Good's buffer, CAS: 29915-38-6, pka=8.4, Active pH 7.7-9.1, made in Japan
In biological experiments, it is important to maintain the pH of the solutions used. Buffers, which are mixtures of weak acids and their appropriate conjugate bases, are commonly used in these experiments. Most biological reactions occur when the buffer is at its effective pH range of 6 to 8. Furthermore, the acids and bases used in the biological reaction should not produce chelates with metal ions. For these reasons, Dr. Good developed several aminoethane and aminopropane sulfonic acids that are now widely used for biological research and analysis. Goods buffers have the following characteristics: 1) High water-solubility. 2) Low cell membrane permeability. 3) Consistent acid-base dissociation constants. 4) Low metal chelating capability. 5) High chemical stability. 6) Low absorption spectra in UV and visible regions.
eMolecules 2,4-Dichloro-N-(2-hydroxyethyl)benzenecarboxamide | 20656-08-0 | MFCD00141691 | 1g
Matrix Scientific | 2,4-Dichloro-N-(2-hydroxyethyl)benzenecarboxamide | 1g | 389721058 | 45569 | 95.000 | 20656-08-0 | MFCD00141691 | 234.080 | C9H9Cl2NO2
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Sigma Aldrich Fine Chemicals Biosciences HEPES BioXtra pH 5.0-6.5 150G
HEPES has been described as one of the best all-purpose buffers available for biological research. At biological pH the molecule is zwitterionic and is effective as a buffer at pH 6.8 to 8.2. HEPES has been used in a wide variety of applications including tissue culture. It is commonly used to buffer cell culture media in air. HEPES finds its usage in in vitro experiments on Mg.
Medchemexpress LLC 1-Piperazinepropanesulfonic acid, β-hydroxy-4-(2-hydroxyethyl)- | 68399-78-0 | 99.9% | 50 G
HEPPSO is a zwitterionic buffer with a working pH range of 7.1-8.5. It has a pKa of 7.84 at 2.0 mM buffer concentration and exhibits a relatively high ability to bind copper(II). It is for research use only.
- Zwitterionic buffer
- Working pH range: 7.1-8.5
- pKa of 7.84 at 2.0 mM buffer concentration
- Relatively high ability to bind copper(II)
Medchemexpress LLC Mops (sodium salt) | 71119-22-7 | 99.83% | 10 G
The product is a buffering agent utilized in biology. It serves to maintain the pH of mammalian cell culture media and notably enhances the thermal stability of BSA.
- Functions as a buffering agent in biological applications.
- Maintains the pH level of mammalian cell culture media.
- Significantly improves the thermal stability of BSA.
- Can be used in the preparation of keratinocyte and microbial culture medium.
- Relatively safe for short-term experiments, though prolonged use may reduce cell viability.
Medchemexpress LLC Bicine | 150-25-4 | 98.7% | 25 G
Bicine is a buffer utilized in physiological research. It is a derivative of the amino acid glycine that enhances the sensitivity of guanosine enzyme histochemical detection.
- Buffer utilized in physiological research
- Derivative of the amino acid glycine
- Enhances the sensitivity of guanosine enzyme histochemical detection
Sigma Aldrich Fine Chemicals Biosciences HEPES | 7365-45-9 | MFCD00006158 | 250 g
HEPES | Purity: ≥99.5% | Mol Wt: 238.30 | 7365-45-9 | MFCD00006158 | 250 g
Sigma Aldrich Fine Chemicals Biosciences MES sodium salt | 71119-23-8 | MFCD00065473 | 100g
MES sodium salt | Purity: 99% | MW: 217.22 | 71119-23-8 | MFCD00065473 | 100g
Boston Bioproducts Inc HEPES Buffer (0.5 M, pH 8.0) - 250ml
The HEPES [4-(2-hydroxyethyl)-1-piperazineethanesulfonic acid)] Buffer is a versatile Good's zwitterionic buffering agent widely used in various applications in biochemistry and molecular biology. It maintains a stable pH in the range of 6.8 to 8.2, making it ideal for various applications at physiological pH, including cell culture, protein purification, isoelectric focusing, enzyme studies, bioprocessing, and live cell imaging.
Quality Biological Inc TAE Buffer 50X, 10 Liters
TAE buffer provides faster electrophoretic migration of linear DNA and better resolution of supercoiled DNA. ¿ TAE Buffer may be more suitable than TBE, for electrophoresis if DNA is to be recovered from the agarose and purified using commercially available DNA purification kits. ¿ Applications: TAE buffer has a relatively low buffering capacity, therefore, periodic replacement of the buffer during prolonged electrophoresis is recommended. ¿ Storage Conditions: 15¿C to 30¿C ¿ Shipping Condition: ambient temperature ¿ Sterile Filtered ¿ DNase RNase and Protease Tested ¿ Concentration: 2 M Tris base and 0.05 M disodium EDTA dihydrate ¿ Product and/or packaging contains recycled/renewable content ¿ Product and/or packaging is recyclable or reduces waste ¿ Product and/or packaging is safer to human and environmental health ¿ ¿ Max Shelf Life: 36 months
Teknova DNA Suspension Buffer, pH 8.0. 100mL, Sterile. DNase/RNase Tested, PCR Grade.
TE buffer is a salt solution that is commonly used to resuspend (or dilute) DNA and RNA. Composed of Tris base and EDTA, these molecules work together to maintain pH and prevent nucleic acids from degradation.