Life Science Buffers
Balanced salt solutions and powders used for molecular, protein, nucleic acid, and cell biology applications including cell culture, electrophoresis, ELISA, and chromatography processes; available in a variety of formulations and grades.
Buffer solutions serve to keep pH (acidity or alkalinity) nearly constant in a variety of chemical and biological applications. For example, a bicarbonate buffer helps maintain the pH of blood, a buffered saline maintains cellular contents at a consistent pH level, and buffers help maintain a narrow pH range for enzymes to function correctly.
Most biological samples used in research are kept at a pH of about 7.4. Common biological buffers used for tissue culture include Dulbeccos phosphate buffered saline (PBS), Tris base, HEPES, MOPS, PIPES, and other formulations developed for specific cell lines or applications. Most buffers used in cell culture are also DNase-, RNase-, and protease-free.
The choice of buffer for a particular biological reaction or site depends on several factors:
- Temperature
- Desired or target pH
- Buffer toxicity (to the system)
- Buffer interactions with other system components
Gold Biotechnology Inc Tris HCl 1 kg
Short for Tris (hydroxymethyl) aminomethane (THAM) hydrochloride, Tris HCl is an organic compound often used in buffer solutions such as TAE or TBE for electrophoresis gels. Tris is highly soluble in water and is useful in the pH range 7.0-9.0. It is used in the preparation of Laemmli buffer, one of the most common SDS-PAGE buffers. In addition, Tris can also be used for many custom running and loading buffers, most often with glycine and SDS. The use of single-junction pH electrodes that contain silver should be avoided when determining the pH of a solution containing Tris buffer.
Teknova 1X PBS with 0.05% Tween 20, pH 7.4. 1000mL, Sterile.
Phosphate-buffered saline (PBS) is a salt solution that is commonly used in physiological and biochemical experiments. It can be used to maintain pH neutrality or to buffer against changes in pH in order to provide optimal conditions for cellular growth and function. The buffering range is between pH 6.8 and 7.4.
Sigma Aldrich Fine Chemicals Biosciences Tris Acetate-EDTA Buffer 51L
TRIS Tris Acetate-EDTA Buffer 51L
Teknova 1M Tris-HCl, pH 8.0 Solution. 1000mL, Sterile.
Tris (tris(hydroxymethyl)aminomethane) is used as a component ofbuffer solutions, especially for solutions ofnucleic acids. The useful buffer range for tris (pH 7–9) is similar to the pH of most living organisms. This, and its low cost, make tris one of the most common buffers in the biology/biochemistry laboratory.
Teknova 1M Tris-HCl, pH 8.3, 1000mL, Sterile.
Tris (tris(hydroxymethyl)aminomethane) is used as a component ofbuffer solutions, especially for solutions ofnucleic acids. The useful buffer range for tris (pH 7–9) is similar to the pH of most living organisms. This, and its low cost, make tris one of the most common buffers in the biology/biochemistry laboratory.
Bioworld Bis-Tris, 500 g
Synonym: 2,2-Bis(hydroxymethyl)-2,2′,2″-nitrilotriethanol, 2-Bis(2-hydroxyethyl)amino-2-(hydroxymethyl)-1,3-propanediol, Bis(2-hydroxyethyl)amino-tris(hydroxymethyl)methane A common biological bufferAn inert, zwitterionic buffer that exhibits only a very small shift in dissociation constantSpecifications:Ultra Pure GradepKa (25°C): 6.5+/-0.2Appearance: SolidBoiling Point : 466.18 °C at 760 mmHg (Predicted)Density : 1.35 g/cm3 (Predicted)Melting Point: 102-105 °CPH Value: 5.8-7.2
![eMolecules Pharmablock / benzyl N-[1-(hydroxymethyl)-2-oxabicyclo[2.1.1]hexan-4-yl]carbamate / 25mg / 551297854 / PBWW273 / 0.000 / 2170372-30-0 / [null] / 263.293 / C14H17NO4](https://assets.fishersci.com/TFS-Assets/CCG/product-images/502462503.JPG-250.jpg)
eMolecules Pharmablock / benzyl N-[1-(hydroxymethyl)-2-oxabicyclo[2.1.1]hexan-4-yl]carbamate / 25mg / 551297854 / PBWW273 / 0.000 / 2170372-30-0 / [null] / 263.293 / C14H17NO4
Pharmablock / benzyl N-[1-(hydroxymethyl)-2-oxabicyclo[2.1.1]hexan-4-yl]carbamate / 25mg / 551297854 / PBWW273 / 0.000 / 2170372-30-0 / [null] / 263.293 / C14H17NO4
Abcam Antigen Retrieval Buffer (100 x Citrate Buffer), 125ML
Antigen retrieval 100X citrate buffer (pH 5.1 - 5.5) is a special solution that enables rehydration and antigen retrieval to be performed in formalin-fixed, paraffin-embedded tissue sections with one step. - Cited in over 25 publications - Contains citrate buffer, 1.5% 2-butoxyethanol, 1% hydrochloric acid - Optimized for citrate buffer-treated antibodies - Contains detergent for paraffin emulsification
The product is subject to the following: Abcam Restricted Use Statement
Medchemexpress LLC P62-mediated mitopha 50mg | 1809031-84-2 | 392.15 | C14H9IN4O2 | 50 MG
P62-mediated mitophagy inducer is a small-molecule activator of p62-dependent mitophagy used in research to stimulate mitochondrial autophagy without recruiting Parkin or collapsing mitochondrial membrane potential.
- Activates p62-mediated mitophagy without Parkin recruitment.
- Promotes mitochondrial autophagy while preserving mitochondrial membrane potential.
- Suitable for autophagy and mitophagy research applications.
- Powder form with defined cold-storage stability for long-term storage.
- Available in small research pack sizes for laboratory use.
- Molecular formula C14H9IN4O2; molecular weight 392.15 g·mol⁻¹.
Alkali Scientific CellPro™ Tris Glycine SDS Buffer, 10X, 4L Cubitainer
Alkali Scientific™ CellPro™ Tris Glycine offers a sterile solution ideal for a variety of biochemical and molecular biology applications. Composed of Tris (Tris(hydroxymethyl)aminomethane) and Glycine, this buffer provides optimal conditions for protein electrophoresis, particularly in SDS-PAGE (Sodium Dodecyl Sulfate Polyacrylamide Gel Electrophoresis) systems. The sterile formulation ensures the purity and integrity of your samples, minimizing the risk of contamination and ensuring reliable results. CellPro™ Tris Glycine buffer is versatile and suitable for use in protein separation, transfer, and analysis techniques, making it an essential component in the laboratory for protein research and characterization. Trust in the quality and consistency of CellPro™ Tris Glycine for your protein electrophoresis experiments.
Abcam Kinase Assay Buffer I, 20ML
Stock solution of buffer used for assaying kinase protein.
The product is subject to the following: Abcam Restricted Use Statement
![eMolecules TERT-BUTYL 6-(HYDROXYMETHYL)-5-OXA-2-AZASPIRO[3.4]OCTANE-2-CARBOXYLATE | 1446012-48-1 | MFCD27986992 | 1g](https://assets.fishersci.com/TFS-Assets/CCG/product-images/502181707.JPG-250.jpg)
eMolecules TERT-BUTYL 6-(HYDROXYMETHYL)-5-OXA-2-AZASPIRO[3.4]OCTANE-2-CARBOXYLATE | 1446012-48-1 | MFCD27986992 | 1g
AstaTech | TERT-BUTYL 6-(HYDROXYMETHYL)-5-OXA-2-AZASPIRO[3.4]OCTANE-2-CARBOXYLATE | 1g | 624127457 | P14281 | 95.000 | 1446012-48-1 | MFCD27986992 | 243.303 | C12H21NO4
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Quality Biological Inc TRIS HCL (1 M), pH 7.0, 500 mL
Tris is a buffer component in molecular biology, tissue culture, and electrophoresis procedures. ¿ Provide convenient and highly purified solutions. ¿ Applications: Tris solutions are formulated into working concentrations for preparing the stacking and resolving portions of native or SDS-PAGE gels.¿ Use Tris-HCl 1 M pH 7.0-7.5 for DEAE Dextran-mediated transfection of non-adherent and adherent cells. ¿ Storage Conditions: 15¿C to 30¿C ¿ Shipping Condition: ambient temperature ¿ Sterile Filtered ¿ DNase RNase and Protease Tested ¿ pH: 7.5¿ 0.1 ¿ Product and/or packaging contains recycled/renewable content ¿ Product and/or packaging is recyclable or reduces waste ¿ Product and/or packaging is safer to human and environmental health ¿ ¿ Max Shelf Life: 36 months
Bioworld Tris-Glycine-SDS (TGS) Running Buffer (10X), 1 L
Product DescriptionTris-Glycine SDS (TGS) running buffer has a concentration of 10X with a pH of 8.3. 1X solution contains 0.025M Tris, 0.192M Glycine, and 0.1% SDS. Product is of ultra pure grade.TG buffers are blended using pure reagents to provide high quality for protein research. This buffer is formulated to ensure exceptional purity along with the absence of nuclease and protease activities. TG buffer is widely used for electroblotting proteins in Western blot procedures and is recommended for use with all nitrocellulose and nylon membranes.
Abcam Salsolinol hydrochloride, Dopamine derivative, 500MG
MW 215.68 g/mol, Purity >99%. Dopamine (ab120565) derivative. Putative endogenous opioid system-activating factor. Antagonizes L-DOPA-mediated hyperactivity. Shows central effects in vivo. .
The product is subject to the following: Abcam Restricted Use Statement