Life Science Buffers
Balanced salt solutions and powders used for molecular, protein, nucleic acid, and cell biology applications including cell culture, electrophoresis, ELISA, and chromatography processes; available in a variety of formulations and grades.
Buffer solutions serve to keep pH (acidity or alkalinity) nearly constant in a variety of chemical and biological applications. For example, a bicarbonate buffer helps maintain the pH of blood, a buffered saline maintains cellular contents at a consistent pH level, and buffers help maintain a narrow pH range for enzymes to function correctly.
Most biological samples used in research are kept at a pH of about 7.4. Common biological buffers used for tissue culture include Dulbeccos phosphate buffered saline (PBS), Tris base, HEPES, MOPS, PIPES, and other formulations developed for specific cell lines or applications. Most buffers used in cell culture are also DNase-, RNase-, and protease-free.
The choice of buffer for a particular biological reaction or site depends on several factors:
- Temperature
- Desired or target pH
- Buffer toxicity (to the system)
- Buffer interactions with other system components
![Research Products International Corp Ethylenediaminetetraacetic acid [EDTA], 500 Grams](https://assets.fishersci.com/TFS-Assets/CCG/product-images/VN00006095-E58100-500.0.JPG-250.jpg)
Research Products International Corp Ethylenediaminetetraacetic acid [EDTA], 500 Grams
Substance Name: EDTA
CAS Number: 60-00-4
Molecular Formula: C10H16N2O8
Molecular Weight: 292.24
Chemical Purity: >99%
Soluble in: Water
Store at: Room Temperature
![Research Products International Corp HEPES, Sodium Salt [N-(2-Hydroxyethyl) piperazine-N'-(2-ethanesulfonic acid)], 1 Kilogram](https://assets.fishersci.com/TFS-Assets/CCG/product-images/VN00006095-H75050-1000.0.JPG-250.jpg)
Research Products International Corp HEPES, Sodium Salt [N-(2-Hydroxyethyl) piperazine-N'-(2-ethanesulfonic acid)], 1 Kilogram
pKa (20°C): 7.45-7.65
pH Range: 6.8-8.2
Research Products International Corp MES, Anhydrous, 100 Grams
- CAS Number: 4432-31-9
- Chemical Formula: C6H13NO4S
- Molecular Weight: 195.24 g/mol
Research Products International Corp MES, Anhydrous, 1 Kilograms
- CAS Number: 4432-31-9
- Chemical Formula: C6H13NO4S
- Molecular Weight: 195.24 g/mol
Research Products International Corp MES, Anhydrous, 25 Grams
- CAS Number: 4432-31-9
- Chemical Formula: C6H13NO4S
- Molecular Weight: 195.24 g/mol
![Research Products International Corp MOPS, Sodium Salt [3-(N-Morpholino)-propanesulfonic acid sodium salt], 5 Kilograms](https://assets.fishersci.com/TFS-Assets/CCG/product-images/VN00006095-M92040-5000.0.JPG-250.jpg)
Research Products International Corp MOPS, Sodium Salt [3-(N-Morpholino)-propanesulfonic acid sodium salt], 5 Kilograms
MOPS Sodium Salt, also known as 3-(N-Morpholino) propanesulfonic acid sodium salt, is a chemical compound that plays a significant role in the life science marketplace. It is a zwitterionic buffer commonly used in biological and biochemical research and is primarily utilized as a buffering agent to maintain a stable pH in various experimental procedures.
It is particularly effective in the pH range of 6.5 to 7.9, making it suitable for applications requiring slightly acidic to neutral pH conditions. MOPS Sodium Salt is commonly used in protein purification, enzyme assays, nucleic acid research, and electrophoresis techniques where a specific pH range needs to be maintained. Its buffering capacity and stability within the desired pH range make it valuable in these applications.
![Research Products International Corp PBS [Phosphate Buffered Saline], 10X Powder Concentrate, White Granular Powder, 1 Liter](https://assets.fishersci.com/TFS-Assets/CCG/product-images/VN00006095-P32200-1000.031.JPG-250.jpg)
Research Products International Corp PBS [Phosphate Buffered Saline], 10X Powder Concentrate, White Granular Powder, 1 Liter
PBS, 10X Powder Concentrate is a highly concentrated phosphate-buffered saline solution used in biology and biochemistry research. It contains sodium chloride, sodium phosphate, and potassium phosphate, which maintain pH and osmolarity. Designed for dilution before use, it offers a convenient and cost-effective way to prepare large volumes of 1X PBS. Once diluted, it serves as an isotonic buffer for cell culture, immunohistochemistry, protein work, and molecular biology. It supports cell and tissue integrity by maintaining stable physiological conditions during washing, staining, or sample preparation.
![Research Products International Corp TAPS, [Tris(hydroxymethyl)-3-aminopropanesulfonic acid], Free Acid, 500 Grams](https://assets.fishersci.com/TFS-Assets/CCG/product-images/VN00006095-T12000-500.0.JPG-250.jpg)
Research Products International Corp TAPS, [Tris(hydroxymethyl)-3-aminopropanesulfonic acid], Free Acid, 500 Grams
pKa(25°:C): 8.4. The working pH range in aqueous solution is 7.7-9.1.
![Research Products International Corp TES, Sodium Salt, [2-Tris(hydroxymethyl) methyl-2-amino-1-ethanesulfonic acid, sodium salt], 100 Grams](https://assets.fishersci.com/TFS-Assets/CCG/product-images/VN00006095-T31020-100.0.JPG-250.jpg)
Research Products International Corp TES, Sodium Salt, [2-Tris(hydroxymethyl) methyl-2-amino-1-ethanesulfonic acid, sodium salt], 100 Grams
TES (N-[Tris(hydroxymethyl)methyl]-2-aminoethanesulfonic acid) Sodium Salt is a commonly used buffering agent in life science research. It helps to maintain a stable pH environment in biological experiments, such as cell culture, protein purification, and enzyme assays.
TES Sodium Salt is advantageous due to its ability to resist changes in pH over a wide range of temperatures, making it suitable for various experimental conditions.
Additionally, its low UV absorbance and minimal interference with biological molecules make it a preferred choice in many biochemical applications
![Research Products International Corp TES, Free Acid [2-Tris(hydroxymethyl)-methyl-2-amino 1-ethanesulfonic acid], 500 Grams](https://assets.fishersci.com/TFS-Assets/CCG/product-images/VN00006095-T31022-500.03.JPG-250.jpg)
Research Products International Corp TES, Free Acid [2-Tris(hydroxymethyl)-methyl-2-amino 1-ethanesulfonic acid], 500 Grams
Substance Name: 2-Tris(hydroxymethyl)-methyl-2-amino 1-ethanesulfonic acid
CAS Number: 7365-44-8
Molecular Formula: C6H15NO6S
Molecular Weight: 229.25 g/mol
Research Products International Corp TRIS, Amino Buffer, Enzyme Grade, 5 Kilograms
- CAS Number: 77-86-1
- Molecular Formula: C4H11NO3
- Molecular Weight: 121.14 g/mol
Research Products International Corp TRIS, ACS Grade, 5 Kilograms
TRIS, also known as tris(hydroxymethyl)aminomethane, is a chemical compound widely used in life science as a buffer.
Research Products International Corp TRIS Buffer Grade, 5 Kilograms
Tris Base Ultra Pure is a commonly used reagent and buffer component. Tris Base, Tris(hydroxymethyl)aminomethane, is a primary amine that acts as a buffering agent to maintain a stable pH in experimental procedures.
It is particularly effective in the pH range of 7.2 to 9.0, making it suitable for applications requiring slightly alkaline to mildly acidic pH conditions. Tris Base Ultra Pure is highly purified to remove impurities and contaminants, ensuring the highest quality for laboratory use.
The ultra-pure form is essential for applications where the presence of impurities can negatively impact experimental results. Tris Base Ultra Pure is commonly used in various molecular biology techniques such as DNA and RNA extraction, protein purification, and enzymatic assays.
It is also utilized in the preparation of electrophoresis buffers and PCR reactions.
Boston Bioproducts Inc MES-SDS Buffer (20X) - 4L
MES-SDS Buffer (20X) - 4L (For Research Use and Further Manufacturing Only)
Boston Bioproducts Inc Sodium Cacodylate Buffer (0.1 M, pH 6.5) - 100ml
Sodium Cacodylate Buffer (0 1 M pH 6 5) - 100ml (For Research Use and Further Manufacturing Only)