Life Science Buffers
Balanced salt solutions and powders used for molecular, protein, nucleic acid, and cell biology applications including cell culture, electrophoresis, ELISA, and chromatography processes; available in a variety of formulations and grades.
Buffer solutions serve to keep pH (acidity or alkalinity) nearly constant in a variety of chemical and biological applications. For example, a bicarbonate buffer helps maintain the pH of blood, a buffered saline maintains cellular contents at a consistent pH level, and buffers help maintain a narrow pH range for enzymes to function correctly.
Most biological samples used in research are kept at a pH of about 7.4. Common biological buffers used for tissue culture include Dulbeccos phosphate buffered saline (PBS), Tris base, HEPES, MOPS, PIPES, and other formulations developed for specific cell lines or applications. Most buffers used in cell culture are also DNase-, RNase-, and protease-free.
The choice of buffer for a particular biological reaction or site depends on several factors:
- Temperature
- Desired or target pH
- Buffer toxicity (to the system)
- Buffer interactions with other system components
![Research Products International Corp MES Monohydrate [2-(N-Morpholino)-ethanesulfonic acid], 500 Grams](https://assets.fishersci.com/TFS-Assets/CCG/product-images/VN00006095-M22040-500.02.JPG-250.jpg)
Research Products International Corp MES Monohydrate [2-(N-Morpholino)-ethanesulfonic acid], 500 Grams
Buffer for use in plant tissue culture.
- pKa (20°C): 6.15
- pH range: 5.5 - 6.7
Research Products International Corp PIPES SODIUM SALT, 100 GRAM
pKa (25°C): 6.8
The working pH range in aqueous solution is 6.5-7.5
![Research Products International Corp TAE 10X Solution [TRIS Acetate EDTA 10X Solution], 4 Liters](https://assets.fishersci.com/TFS-Assets/CCG/product-images/VN00006095-T60010-4000.011.JPG-250.jpg)
Research Products International Corp TAE 10X Solution [TRIS Acetate EDTA 10X Solution], 4 Liters
TAE 10X Solution is a concentrated Tris-acetate-EDTA buffer used in molecular biology for DNA and RNA electrophoresis. When diluted to 1X, it maintains pH (~8.3) and low ionic strength. It supports clear nucleic acid separation, extended run times, and protects samples from degradation by chelating divalent metal ions like Mg²⁺.
Substance Name: [TRIS Acetate EDTA 10X Solution]
10X solution contains:
TRIS-Base: 0.4 M
Acetic Acid: 0.2 M
EDTA: 10 mM
pH (10X Solution (@25°C): 8.2-8.4
![Research Products International Corp TES, Free Acid [2-Tris(hydroxymethyl)-methyl-2-amino 1-ethanesulfonic acid], 100 Grams](https://assets.fishersci.com/TFS-Assets/CCG/product-images/VN00006095-T31022-100.0.JPG-250.jpg)
Research Products International Corp TES, Free Acid [2-Tris(hydroxymethyl)-methyl-2-amino 1-ethanesulfonic acid], 100 Grams
Substance Name: 2-Tris(hydroxymethyl)-methyl-2-amino 1-ethanesulfonic acid
CAS Number: 7365-44-8
Molecular Formula: C6H15NO6S
Molecular Weight: 229.25 g/mol
![Research Products International Corp HEPES, Sodium Salt [N-(2-Hydroxyethyl) piperazine-N'-(2-ethanesulfonic acid)], 100 Grams](https://assets.fishersci.com/TFS-Assets/CCG/product-images/VN00006095-H75050-100.0.JPG-250.jpg)
Research Products International Corp HEPES, Sodium Salt [N-(2-Hydroxyethyl) piperazine-N'-(2-ethanesulfonic acid)], 100 Grams
pKa (20°C): 7.45-7.65
pH Range: 6.8-8.2
![Research Products International Corp HEPES, Free Acid [N-(2-Hydroxyethyl) piperazine N'-(2-ethanesulfonic acid)], 5 Kilograms](https://assets.fishersci.com/TFS-Assets/CCG/product-images/VN00006095-H75030-5000.0.JPG-250.jpg)
Research Products International Corp HEPES, Free Acid [N-(2-Hydroxyethyl) piperazine N'-(2-ethanesulfonic acid)], 5 Kilograms
HEPES, Free Acid (4-(2-Hydroxyethyl)piperazine-1-ethanesulfonic acid), is a zwitterionic buffer.
In the life science field, HEPES, Free Acid, is primarily utilized as a buffering agent to maintain a stable pH in various experimental procedures. It is particularly effective in the pH range of 6.8 to 8.2, making it suitable for applications requiring slightly acidic to neutral pH conditions.
HEPES, Free Acid, is commonly used in cell culture media, protein purification, and enzyme assays where a specific pH range needs to be maintained. Its buffering capacity and stability within the desired pH range make it valuable in these applications.
One of the significant advantages of HEPES, Free Acid, is its compatibility with biological systems and enzymes. It exhibits minimal interference with enzymatic activity and other biological processes, ensuring reliable and accurate results.
![Research Products International Corp HEPPSO [N-(2-Hydroxyethyl) piperazine-N'-(2-hydroxypropanesulfonic acid)], 100 Grams](https://assets.fishersci.com/TFS-Assets/CCG/product-images/VN00006095-H75070-100.0.JPG-250.jpg)
Research Products International Corp HEPPSO [N-(2-Hydroxyethyl) piperazine-N'-(2-hydroxypropanesulfonic acid)], 100 Grams
HEPPSO (N-(2-Hydroxyethyl)piperazine-N'-(3-propanesulfonic acid)) is a zwitterionic buffering agent commonly used particularly in biochemical and molecular biology research. It is valued for its ability to maintain a stable pH in a variety of biological assays and experiments.
HEPPSO is often preferred for its buffering capacity over a wide pH range, typically between pH 6.8 to 8.2, making it suitable for various applications such as protein purification, enzyme assays, cell culture, and electrophoresis.
Additionally, HEPPSO is less likely to interfere with biological processes compared to some other buffering agents, making it a versatile choice for maintaining optimal conditions in biological experiments.
![Research Products International Corp HEPES, Sodium Salt [N-(2-Hydroxyethyl) piperazine-N'-(2-ethanesulfonic acid)], 500 Grams](https://assets.fishersci.com/TFS-Assets/CCG/product-images/VN00006095-H75050-500.04.JPG-250.jpg)
Research Products International Corp HEPES, Sodium Salt [N-(2-Hydroxyethyl) piperazine-N'-(2-ethanesulfonic acid)], 500 Grams
pKa (20°C): 7.45-7.65
pH Range: 6.8-8.2
Research Products International Corp TRIS-BORATE-EDTA, 10X SOLUTION, 4 LITER
TBE is a common buffer used in gel electrophoresis.
Supplied as a 1X concentrated solution containing:
89mM Boric Acid
2mM EDTA disodium salt
pH (1X solution @ 25°:C): 8.3+0.1
![Research Products International Corp MOPS, Sodium Salt [3-(N-Morpholino)-propanesulfonic acid sodium salt], 500 Grams](https://assets.fishersci.com/TFS-Assets/CCG/product-images/VN00006095-M92040-500.0.JPG-250.jpg)
Research Products International Corp MOPS, Sodium Salt [3-(N-Morpholino)-propanesulfonic acid sodium salt], 500 Grams
MOPS Sodium Salt, also known as 3-(N-Morpholino) propanesulfonic acid sodium salt, is a chemical compound that plays a significant role in the life science marketplace. It is a zwitterionic buffer commonly used in biological and biochemical research and is primarily utilized as a buffering agent to maintain a stable pH in various experimental procedures.
It is particularly effective in the pH range of 6.5 to 7.9, making it suitable for applications requiring slightly acidic to neutral pH conditions. MOPS Sodium Salt is commonly used in protein purification, enzyme assays, nucleic acid research, and electrophoresis techniques where a specific pH range needs to be maintained. Its buffering capacity and stability within the desired pH range make it valuable in these applications.
![Research Products International Corp MES Monohydrate [2-(N-Morpholino)-ethanesulfonic acid], 1 Kilogram](https://assets.fishersci.com/TFS-Assets/CCG/product-images/VN00006095-M22040-1000.0.JPG-250.jpg)
Research Products International Corp MES Monohydrate [2-(N-Morpholino)-ethanesulfonic acid], 1 Kilogram
Buffer for use in plant tissue culture.
- pKa (20°C): 6.15
- pH range: 5.5 - 6.7
![Research Products International Corp HEPES, Free Acid [N-(2-Hydroxyethyl) piperazine N'-(2-ethanesulfonic acid)], 100 Grams](https://assets.fishersci.com/TFS-Assets/CCG/product-images/VN00006095-H75030-100.0.JPG-250.jpg)
Research Products International Corp HEPES, Free Acid [N-(2-Hydroxyethyl) piperazine N'-(2-ethanesulfonic acid)], 100 Grams
HEPES, Free Acid (4-(2-Hydroxyethyl)piperazine-1-ethanesulfonic acid), is a zwitterionic buffer.
In the life science field, HEPES, Free Acid, is primarily utilized as a buffering agent to maintain a stable pH in various experimental procedures. It is particularly effective in the pH range of 6.8 to 8.2, making it suitable for applications requiring slightly acidic to neutral pH conditions.
HEPES, Free Acid, is commonly used in cell culture media, protein purification, and enzyme assays where a specific pH range needs to be maintained. Its buffering capacity and stability within the desired pH range make it valuable in these applications.
One of the significant advantages of HEPES, Free Acid, is its compatibility with biological systems and enzymes. It exhibits minimal interference with enzymatic activity and other biological processes, ensuring reliable and accurate results.
Alkali Scientific Neutralizing Buffered Peptone Water (NBPW), Sterile, Pre Enrichment Medium with Disinfectant Neutralizers, Ready to Use (Bottled) Microbiology Diluent, 400mL, 20/Case
Neutralizing Buffered Peptone Water (NBPW), Sterile, Pre Enrichment Medium with Disinfectant Neutralizers, Ready to Use (Bottled) Microbiology Diluent, 400mL, 20/Case. This product is formulated for neutralizing disinfectants and enriching microbiological media. The sterile solution is ready-to-use and helps enhance the accuracy of lab tests by eliminating disinfectants in sample preparations. Suitable for use in microbiological analysis.