Life Science Buffers
Balanced salt solutions and powders used for molecular, protein, nucleic acid, and cell biology applications including cell culture, electrophoresis, ELISA, and chromatography processes; available in a variety of formulations and grades.
Buffer solutions serve to keep pH (acidity or alkalinity) nearly constant in a variety of chemical and biological applications. For example, a bicarbonate buffer helps maintain the pH of blood, a buffered saline maintains cellular contents at a consistent pH level, and buffers help maintain a narrow pH range for enzymes to function correctly.
Most biological samples used in research are kept at a pH of about 7.4. Common biological buffers used for tissue culture include Dulbeccos phosphate buffered saline (PBS), Tris base, HEPES, MOPS, PIPES, and other formulations developed for specific cell lines or applications. Most buffers used in cell culture are also DNase-, RNase-, and protease-free.
The choice of buffer for a particular biological reaction or site depends on several factors:
- Temperature
- Desired or target pH
- Buffer toxicity (to the system)
- Buffer interactions with other system components
Sigma Aldrich Fine Chemicals Biosciences TAPS BioXtra 99.5 titratio100G
TAPS9 TAPS BioXtra 99.5 titratio100G
Biotang Inc HEPES Buffered Saline 5X, pH 6.5. 125 mM HEPES, 750 mM Sodium Chloride, pH 6.5. Prepared in 18.2 megohms water and filtered through 0.22 micron filter. BB-545
HEPES Buffered Saline 5X, pH 6.5. 125 mM HEPES, 750 mM Sodium Chloride, pH 6.5. Prepared in 18.2 megohms water and filtered through 0.22 micron filter. BB-545.
Medchemexpress LLC Ada disodium | 41689-31-0 | 98.0% | 5 G
ADA (disodium) is a buffer salt derived from adenosine deaminase, commonly employed in biochemical research, particularly for preparing enzyme buffers.
- Used in biochemical research.
- Specifically used in the preparation of enzyme buffers.
Sigma Aldrich Fine Chemicals Biosciences ACES BioXtra, >=99.0% (titration) | 7365-82-4 | MFCD00008030 | 100G
ACES BioXtra, >=99.0% (titration) | Purity: >=99.0% (titration) | Mol Wt: 182.2 | 7365-82-4 | MFCD00008030 | 100G
Research Products International Corp HEPES, Sodium Salt, 250 G
pKa (20°C): 7.45-7.65
pH Range: 6.8-8.2
Chem-Impex International, Inc. 4-(2-Hydroxyethyl)-1-piperazineethanesulfonic acid | 7365-45-9 | MFCD00006158 | 250G
4-(2-Hydroxyethyl)-1-piperazineethanesulfonic acid, 7365-45-9, MFCD00006158, 250G
Boston Bioproducts Inc HEPES Buffer (1 M, pH 8.5) - 500ml
HEPES Buffer (1 M pH 8 5) - 500ml (For Research Use and Further Manufacturing Only)
AG Scientific Inc MOPS Free Acid 1 KG
MOPS, also known as 3-(N-morpholino)propanesulfonic acid, is one of Good’s buffers. It is N-substituted amino sulfonic acid with a morpholinic ring and has zwitterionic and inert properties. MOPS buffer is broadly used in biological and biochemical studies. It can be mixed with MOPS sodium salt to attain desired pH.MOPS is recommended as an agent for non-coordinating buffers in solutions with metal ions because it hardly forms a complex with most metal ions such as Cu, Ni, Mn, Zn and Co ions. The Good's buffer interferes with the Folin protein assay and partially decomposes when autoclaved in the presence of glucose. MOPS can be used as a Good’s buffer because it has low UV absorptivity, minimal reactivity, stable pH and is soluble in water.
Medchemexpress LLC MOPS sodium salt | 71119-22-7 | 99.8% | 500 G
MOPS sodium salt is a buffering agent used in biology. MOPS buffer maintains the pH of mammalian cell culture media. It significantly improves the thermal stability of BSA. MOPS sodium salt is relatively safe for short-term experiments, but it may significantly reduce cell viability after 4 hours, so it is recommended to limit the experiment duration to 4-12 hours.
- Purity: 99.8%
- Appearance: Solid
- Color: White to off-white
- Storage: 4°C, sealed, away from moisture. In solvent: -80°C for 6 months; -20°C for 1 month (sealed, away from moisture).
- Solubility in H2O: 100 mg/mL (needs ultrasonic)
- Solubility in DMSO: 50 mg/mL (needs ultrasonic; use newly opened DMSO)
Alkali Scientific Murashige & Skoog Medium with MES Buffer and Vitamins, 49 Grams of Powder, Makes 10 Liters of Solution
Murashige & Skoog Medium with MES Buffer and Vitamins (10 liters, 49 grams of powder) is designed for plant tissue culture applications that require large volumes of prepared solution. This medium is enriched with essential nutrients, growth regulators, and MES buffer, which helps to stabilize the pH during plant tissue growth. The formula ensures optimal growth conditions for plant cells, tissues, and organs in vitro, making it ideal for shoot multiplication, regeneration, and propagation experiments. It is commonly used in commercial plant propagation, agricultural research, and plant breeding. The large 10-liter yield makes this medium suitable for labs and research facilities with higher throughput needs.
Research Products International Corp MES, Anhydrous, 1 Kilograms
- CAS Number: 4432-31-9
- Chemical Formula: C6H13NO4S
- Molecular Weight: 195.24 g/mol