Life Science Buffers
Balanced salt solutions and powders used for molecular, protein, nucleic acid, and cell biology applications including cell culture, electrophoresis, ELISA, and chromatography processes; available in a variety of formulations and grades.
Buffer solutions serve to keep pH (acidity or alkalinity) nearly constant in a variety of chemical and biological applications. For example, a bicarbonate buffer helps maintain the pH of blood, a buffered saline maintains cellular contents at a consistent pH level, and buffers help maintain a narrow pH range for enzymes to function correctly.
Most biological samples used in research are kept at a pH of about 7.4. Common biological buffers used for tissue culture include Dulbeccos phosphate buffered saline (PBS), Tris base, HEPES, MOPS, PIPES, and other formulations developed for specific cell lines or applications. Most buffers used in cell culture are also DNase-, RNase-, and protease-free.
The choice of buffer for a particular biological reaction or site depends on several factors:
- Temperature
- Desired or target pH
- Buffer toxicity (to the system)
- Buffer interactions with other system components
Bioworld TAE Buffer (10X), 4 L
Product DescriptionTAE (Tris-Acetate-EDTA) buffer liquid concentrate has a 10X concentration and is prepared with 18 megohm water. It has a pH of 8.3 ± 0.2. A concentration of 1X contains 0.4M Tris acetate and 0.01M EDTA. TAE is used for electrophoresis of nucleic acids in agarose and polyacrylamide gels. It can be used for both genomic and large supercoiled DNA, and can also be used as both a running buffer and a gel preparation buffer. It is recommended for resolution of RNA and DNA fragments larger than 1500 bp, for genomic DNA and for large supercoiled DNA. TAE buffer has a relatively low buffering capacity and therefore replacement of the buffer during prolonged electrophoresis is recommended.
![eMolecules 1446012-48-1 | TERT-BUTYL 6-(HYDROXYMETHYL)-5-OXA-2-AZASPIRO[3.4]OCTANE-2-CARBOXYLATE | MFCD27986992 | 1g](https://assets.fishersci.com/TFS-Assets/CCG/product-images/502181707.JPG-250.jpg)
eMolecules 1446012-48-1 | TERT-BUTYL 6-(HYDROXYMETHYL)-5-OXA-2-AZASPIRO[3.4]OCTANE-2-CARBOXYLATE | MFCD27986992 | 1g
Medchem Express | Deschloroclozapine | 10mg | 446270896 | HY-42110 | 1977-07-7 | MFCD01730958 | 292.386 | C18H20N4
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Alkali Scientific TRIS, Amino Buffer, Enzyme Grade, 5 Kilograms
TRIS Amino (Enzyme Grade) buffer is a high-purity solution designed specifically for enzyme-related applications in molecular biology and biochemistry. This 5kg package provides an ample supply of this reagent, which is widely used for preparing enzyme buffers in assays such as enzyme-linked immunosorbent assays (ELISA), protein assays, and PCR. The TRIS Amino buffer maintains stable pH conditions critical for optimal enzyme activity, and its enzyme-grade quality ensures minimal impurities that could interfere with biochemical reactions. This buffer is ideal for use in experiments where precise pH control is required for enzymatic reactions, as well as for preparing buffers for enzyme activity studies and protein purification.
eMolecules 354587-72-7 | tert-Butyl 6-(hydroxymethyl)-1h-indole-1-carboxylate | Combi-Blocks | MFCD19103503 | 247.294 | C14H17NO3 | 97.000 | CC(C)(C)OC(=O)n1ccc2ccc(CO)cc12 | 1g | 267192141
tert-Butyl 6-(hydroxymethyl)-1h-indole-1-carboxylate | Combi-Blocks | 354587-72-7 | MFCD19103503 | 247.294 | C14H17NO3 | 97.000 | CC(C)(C)OC(=O)n1ccc2ccc(CO)cc12 | 1g | 267192141
Biotang Inc Laemmli Buffer with PYRONINY, 4X Reducing, 25 ml
Laemmli Buffer with PYRONINY, 4X Reducing, 25 ml.
TARGETMOL CHEMICALS INC NVP-TAE 684 10MG
Also available in 2 mg 5 mg 25 mg 50 mg 100 mg 1 mL 10 mM (in DMSO) and bulk. Please contact Fisher for quotes. NVP-TAE 684 (TAE684) is a excellently effective and specific ALK inhibitor(IC50=3 nM). purity: 99%
Pfanstiehl Inc Tris Hydrochloride (Tris HCl) High Purity, Low Endotoxin, Low Metals (T-154) 5kg GMP CAS: 1185-53-1 Injectable Excipient Grade
Pfanstiehl Tris HCL, High Purity, Low Endotoxin, Low Metals, is a highly purified buffer component that has been specifically designed and formulated to remove impurities such as endotoxin, trace metals, and nitrosamines. Manufactured in the USA under ICH-Q7 cGMP conditions, Pfanstiehl Tris HCl meets parenteral (injectable) excipient grade standards, making it ideal for pharmaceutical, biologic, and vaccine bioprocessing and formulation. cGMP , pharmaceutical grade .
Kd Medical Inc TAE 10X 1L BOTTLE
TAE Buffer (Tris Actetate EDTA) is the most commonly used buffer for agarose DNA electrophoresis. 10X concentrated ready to be diluted to a 1X working solution. One 1 liter bottle.
Ethos Biosciences Dilurex 1M Tris-HCL Buffer, 1000ml, Ethos
Dilurex 1M Tris-HCL 10X Buffer is a classic laboratory buffer at pH 7.4. It does not include a saline ionic component and is free to be mixed with other components as needed in your laboratory. kip the tedium of weighing powders and stocking raw materials that can expire. Our buffer is convenient, ready to use, and will save you hours in the lab, letting you focus on breakthroughs rather than buffer prep. Thousands of researchers trust Ethos Biosciences buffers for their proven track record in top-tier publications and protocols - upgrade your productivity today.
BLD PHARMATECH CO LIMITED 4-(Hydroxymethyl)-4-methyltetrahydropyran, CAS Number: 502609-47-4, 1g, Purity: 97%
4-(Hydroxymethyl)-4-methyltetrahydropyran, CAS Number: 502609-47-4, 1g, Purity: 97%