Life Science Buffers
Balanced salt solutions and powders used for molecular, protein, nucleic acid, and cell biology applications including cell culture, electrophoresis, ELISA, and chromatography processes; available in a variety of formulations and grades.
Buffer solutions serve to keep pH (acidity or alkalinity) nearly constant in a variety of chemical and biological applications. For example, a bicarbonate buffer helps maintain the pH of blood, a buffered saline maintains cellular contents at a consistent pH level, and buffers help maintain a narrow pH range for enzymes to function correctly.
Most biological samples used in research are kept at a pH of about 7.4. Common biological buffers used for tissue culture include Dulbeccos phosphate buffered saline (PBS), Tris base, HEPES, MOPS, PIPES, and other formulations developed for specific cell lines or applications. Most buffers used in cell culture are also DNase-, RNase-, and protease-free.
The choice of buffer for a particular biological reaction or site depends on several factors:
- Temperature
- Desired or target pH
- Buffer toxicity (to the system)
- Buffer interactions with other system components
United States Biological Corporation DULBECCO MEM DMEM PWDR D980028
NC3314668 DULBECCO MEM DMEM PWDR D980028
Electron Microscopy Sciences Hank's Balanced Salts Formula I 500 ML
All of our Hanks' Balanced Salt Solutions contain: Potassium Chloride, Potassium Phosphate Monobasic, Sodium Chloride, Sodium Phosphate Dibasic, and D-Glucose. Some formulations may contain additional components. Use the table below as a quick reference for identifying the one that best suits your application. All solutions are prepared in 1X strength.
Apexbio Technology LLC Annexin V-HF647/7-AAD Apoptosis Kit 20assays
Annexin V is a Ca2 -dependent phospholipid-binding protein that specifically binds to phosphatidylserine (PS) Normally PS is localized on the inner leaflet of the cell membrane lipid bilayer During the early stages of apoptosis PS translocates from the inner leaflet to the outer surface of the cell membrane exposing it to the extracellular environment Due to Annexin V s high affinity for PS fluorescently labeled Annexin V (HF647) can be used as a probe in combination with flow cytometry or fluorescence microscopy for detecting apoptosis and the staining process requires only 10-20 minutes 7-AAD specifically binds double-stranded DNA emitting red fluorescence Normally 7-AAD cannot penetrate intact cell membranes However in late-apoptotic and dead cells membrane integrity is compromised allowing 7-AAD to enter and stain nuclear DNA Co-staining with Annexin V-HF647 and 7-AAD thus differentiates cells in various apoptotic stages and dead cells
Apexbio Technology LLC Annexin V-HF488/7-AAD Apoptosis Kit 20assays
Annexin V is a Ca2 -dependent phospholipid-binding protein that specifically binds to phosphatidylserine (PS) Normally PS is localized on the inner leaflet of the cell membrane lipid bilayer During the early stages of apoptosis PS translocates from the inner leaflet to the outer surface of the cell membrane exposing it to the extracellular environment Due to Annexin V s high affinity for PS fluorescently labeled Annexin V (HF488) can be used as a fluorescent probe to detect apoptotic cells through flow cytometry or fluorescence microscopy with staining taking only 10-20 minutes 7-AAD can specifically bind to double-stranded DNA resulting in red fluorescence Normally 7-AAD cannot pass through intact cell membranes however it can penetrate the cell membranes of late-stage apoptotic and dead cells staining their nuclei Combined staining with Annexin V-HF488 and 7-AAD allows differentiation of cells in various stages of apoptosis and dead cells
Sigma Aldrich Fine Chemicals Biosciences Dulbeccos Modified Eagles500ML
This modification has been shown to improve the stability of the medium.
Ethos Biosciences Dilurex 10X PBS Buffer, 100ml, Ethos
Dilurex 10X PBS is a concentrated solution of phosphate buffered saline intended as a stock solution. Save space by keeping 10x concentrate and dilute to 1x pH 7.4 working concentration when needed by mixing 1 part 10X PBS with 9 parts distilled water. Skip the tedium of weighing powders and stocking raw materials that can expire. Our PBS is convenient, ready to use, and will save you hours in the lab, letting you focus on breakthroughs rather than buffer prep. Thousands of researchers trust Ethos Biosciences 10X PBS for its proven track record in top-tier publications and protocols - upgrade your productivity today.
Teknova 1X PBS Solution, pH 7.4, 200L Bag, Sterile.
Phosphate-buffered saline (PBS) is a salt solution that is commonly used in physiological and biochemical experiments. It can be used to maintain pH neutrality or to buffer against changes in pH in order to provide optimal conditions for cellular growth and function. The buffering range is between pH 6.8 and 7.4.
Medchemexpress LLC Phosphate-Buffered Saline | 500 ML
Phosphate-Buffered Saline (PBS) is a balanced salt solution designed for various cell culture applications. These applications include washing cells, diluting cells, and preparing reagents. The standard base specification for this product is 500 mL, with larger sizes being incremental volumes of this base.
- Filtered and ready to use for general cell culture purposes.
- Free of Mg2+, Ca2+, and phenol red.
- pH is 7.2-7.4.
Alkali Scientific CellPro™ DPBS Buffer, 1X, with Calcium and Magnesium, 1L, 10/Case
Phosphate Buffered Saline is a buffer solution commonly used in biological research. PBS has many uses because it is isotonic and non-toxic to most cells. These uses include substance dilution and cell container rinsing.
Alkali Scientific CellPro™ DPBS, 10X, 500mL
Experience superior performance and convenience with Alkali Scientific™ CellPro™ 10X DPBS, Dulbecco’s Phosphate Buffered Saline. This concentrated buffer solution offers ten times the strength of standard DPBS, providing researchers with a cost-effective and time-saving option for buffer preparation. With its precisely balanced formulation and high purity, CellPro™ 10X DPBS ensures consistent results in cell culture, molecular biology, and immunocytochemistry applications. Trust Alkali Scientific™ CellPro™ 10X DPBS for reliable performance and reproducibility in your laboratory workflows.
Boston Bioproducts Inc 10X Dulbecco's Phosphate Buffered Saline, DPBS (no Calcium, no Magnesium) - 2x1L
Bioprocess Grade - 10X Dulbecco s Phosphate Buffered Saline DPBS (no Calcium no Magnesium) - 2x1L (For Research Use and Further Manufacturing Only)
Sigma Aldrich Fine Chemicals Biosciences Dulbeccos Modified Eagles1L
This DMEM-Hi glucose medium is a 1x complete medium with no added factors (common modifications) such as L-alanyl-L-glutamine HEPES or sodium pyruvate. It differs from the original DMEM-Hi formulation wherein pyridoxine is substituted for pyridoxal. Pyridoxal is an unstable component of media. This medium requires supplementation with L-glutamine or L-alanyl-L-glutamine.
Apexbio Technology LLC Deacetylase Inhibitor Cocktail (100X in 70% DMSO) 100x1ml
Deacetylase Inhibitor Cocktail is composed of several compounds that act synergistically to maintain the acetylation status of molecules The balance between acetyltransferase and deacetylase activities impacts chromatin compaction transcription factor activity and protein aggregation and the variations of these modifications have garnered considerable interest from researchers Our product Deacetylase Inhibitor Cocktail is a valuable tool for investigating these changes The Deacetylation Inhibition Cocktail includes four active components 40 M Trichostatin A 1 mM EX-527 400 mM nicotinamide and 200 mM sodium butyrate These compounds are dissolved in 70% DMSO each with distinct specific actions Trichostatin A inhibits class I/II HDACs EX-527 selectively inhibits SIRT1 with weaker activity against SIRT2 and SIRT3 Nicotinamide acts as an inhibitor of sirtuins (SIRT1-7 class III HDACs) Sodium butyrate competitively inhibits class I/II HDACs
Alkali Scientific CellPro™ PBS Buffer, 10X, pH 7.4, 1L, 10/cs
Alkali Scientific™ CellPro™ PBS (Phosphate Buffered Saline) provides researchers with a reliable and consistent solution essential for various molecular and cellular biology applications. This meticulously prepared buffer is formulated to maintain a stable pH and osmolarity, ensuring optimal conditions for cell culture, protein assays, immunohistochemistry, and many other biological techniques. With its phosphate-based formulation, CellPro™ PBS offers excellent buffering capacity, making it suitable for maintaining the physiological pH of biological samples and preserving their integrity. Whether used for cell washing, sample dilution, or buffer exchange, CellPro™ PBS guarantees exceptional performance and reproducibility in every experiment. Trust in the quality and reliability of CellPro™ PBS to support your research needs and enable precise and consistent results.
Alkali Scientific CellPro™ DPBS Buffer 10X, 1L
Experience superior performance and convenience with Alkali Scientific™ CellPro™ 10X DPBS, Dulbecco’s Phosphate Buffered Saline. This concentrated buffer solution offers ten times the strength of standard DPBS, providing researchers with a cost-effective and time-saving option for buffer preparation. With its precisely balanced formulation and high purity, CellPro™ 10X DPBS ensures consistent results in cell culture, molecular biology, and immunocytochemistry applications. Trust Alkali Scientific™ CellPro™ 10X DPBS for reliable performance and reproducibility in your laboratory workflows.