Life Science Buffers
Balanced salt solutions and powders used for molecular, protein, nucleic acid, and cell biology applications including cell culture, electrophoresis, ELISA, and chromatography processes; available in a variety of formulations and grades.
Buffer solutions serve to keep pH (acidity or alkalinity) nearly constant in a variety of chemical and biological applications. For example, a bicarbonate buffer helps maintain the pH of blood, a buffered saline maintains cellular contents at a consistent pH level, and buffers help maintain a narrow pH range for enzymes to function correctly.
Most biological samples used in research are kept at a pH of about 7.4. Common biological buffers used for tissue culture include Dulbeccos phosphate buffered saline (PBS), Tris base, HEPES, MOPS, PIPES, and other formulations developed for specific cell lines or applications. Most buffers used in cell culture are also DNase-, RNase-, and protease-free.
The choice of buffer for a particular biological reaction or site depends on several factors:
- Temperature
- Desired or target pH
- Buffer toxicity (to the system)
- Buffer interactions with other system components
![eMolecules ChemScene / tert-Butyl 4-(2-hydroxyethyl)-1H-pyrazole-1-carboxylate / 100mg / 761228845 / CS-0613224 / 0.000 / 143282-05-7 / [null] / 212.249 / C10H16N2O3](https://assets.fishersci.com/TFS-Assets/CCG/product-images/502472486.JPG-250.jpg)
eMolecules ChemScene / tert-Butyl 4-(2-hydroxyethyl)-1H-pyrazole-1-carboxylate / 100mg / 761228845 / CS-0613224 / 0.000 / 143282-05-7 / [null] / 212.249 / C10H16N2O3
ChemScene / tert-Butyl 4-(2-hydroxyethyl)-1H-pyrazole-1-carboxylate / 100mg / 761228845 / CS-0613224 / 0.000 / 143282-05-7 / [null] / 212.249 / C10H16N2O3
Sigma Aldrich Fine Chemicals Biosciences HEPES 99.5 (titration), 7365-45-9, MFCD00006158
HEPES 99.5 (titration)
Sigma Aldrich Fine Chemicals Biosciences HEPES solution | 7365-45-9 | MFCD00006158 | 500ml
HEPES solution | Mol Wt: 238.306 | 7365-45-9 | MFCD00006158 | 500ml
Chem-Impex International, Inc. 3-Cyclohexylamino-1-propanesulfonic acid | 1135-40-6 | MFCD00003837 | 100G
3-Cyclohexylamino-1-propanesulfonic acid, 1135-40-6, MFCD00003837, 100G
Sigma Aldrich Fine Chemicals Biosciences MOPS anhydrous, free-flowing, Redi-Dri(TM), >=99.5% | 1132-61-2 | MFCD00006183 | 2.5KG
MOPS anhydrous, free-flowing, Redi-Dri(TM), >=99.5% | Purity: >=99.5% | Mol Wt: 209.26 | 1132-61-2 | MFCD00006183 | 2.5KG
Biotang Inc PIPES Lysis Buffer with Triton (2X), 125 ml
PIPES Lysis Buffer with Triton (2X), 125 ml.
Sigma Aldrich Fine Chemicals Biosciences BICINE Redi DriTM 99 free 500G
BICINE Redi DriTM 99 free 500G
Boston Bioproducts Inc MES Buffer (1 M, pH 5.0) - 500ml
MES Buffer (1 M pH 5 0) - 500ml (For Research Use and Further Manufacturing Only)
![Research Products International Corp TES, Sodium Salt, [2-Tris(hydroxymethyl) methyl-2-amino-1-ethanesulfonic acid, sodium salt], 100 Grams](https://assets.fishersci.com/TFS-Assets/CCG/product-images/VN00006095-T31020-100.0.JPG-250.jpg)
Research Products International Corp TES, Sodium Salt, [2-Tris(hydroxymethyl) methyl-2-amino-1-ethanesulfonic acid, sodium salt], 100 Grams
TES (N-[Tris(hydroxymethyl)methyl]-2-aminoethanesulfonic acid) Sodium Salt is a commonly used buffering agent in life science research. It helps to maintain a stable pH environment in biological experiments, such as cell culture, protein purification, and enzyme assays.
TES Sodium Salt is advantageous due to its ability to resist changes in pH over a wide range of temperatures, making it suitable for various experimental conditions.
Additionally, its low UV absorbance and minimal interference with biological molecules make it a preferred choice in many biochemical applications
Sigma Aldrich Fine Chemicals Biosciences POPSO hydrate >=99% (titration) | 68189-43-5 (anhydrous) | 100G
POPSO hydrate >=99% (titration) | Purity: >=99% (titration) | 68189-43-5 (anhydrous) | 100G
Crescent Chemical Co Inc MORPHOLINOETHANE SULFONIC ACID
Morpholinoethane sulfonic acid analytical grade pKa 20 = 6.15. Buffering substance for biochemistry and molecular biology (1). Physical constants (2). Size - 1KG Storage Conditions - +15 °C TO +30 °C Catalog Number - 29834.03 CAS 4432-31-9
Medchemexpress LLC HEPES, NaCl, Sucrose, pH 7.2 buffer | 100 ML
This physiological buffer is composed of 20 mM HEPES, 150 mM NaCl, and 240 mM Sucrose, adjusted to pH 7.2. It is primarily used to resuspend human erythrocyte ghost preparations.
- Physiological buffer
- Composed of HEPES, NaCl, and Sucrose
- Adjusted to pH 7.2
- Used to resuspend human erythrocyte ghost preparations