Life Science Buffers
Balanced salt solutions and powders used for molecular, protein, nucleic acid, and cell biology applications including cell culture, electrophoresis, ELISA, and chromatography processes; available in a variety of formulations and grades.
Buffer solutions serve to keep pH (acidity or alkalinity) nearly constant in a variety of chemical and biological applications. For example, a bicarbonate buffer helps maintain the pH of blood, a buffered saline maintains cellular contents at a consistent pH level, and buffers help maintain a narrow pH range for enzymes to function correctly.
Most biological samples used in research are kept at a pH of about 7.4. Common biological buffers used for tissue culture include Dulbeccos phosphate buffered saline (PBS), Tris base, HEPES, MOPS, PIPES, and other formulations developed for specific cell lines or applications. Most buffers used in cell culture are also DNase-, RNase-, and protease-free.
The choice of buffer for a particular biological reaction or site depends on several factors:
- Temperature
- Desired or target pH
- Buffer toxicity (to the system)
- Buffer interactions with other system components
Quality Biological Inc TAE Buffer 50X, CS 10 X 1000 mL
TAE buffer provides faster electrophoretic migration of linear DNA and better resolution of supercoiled DNA. ¿ TAE Buffer may be more suitable than TBE, for electrophoresis if DNA is to be recovered from the agarose and purified using commercially available DNA purification kits. ¿ Applications: TAE buffer has a relatively low buffering capacity, therefore, periodic replacement of the buffer during prolonged electrophoresis is recommended. ¿ Storage Conditions: 15¿C to 30¿C ¿ Shipping Condition: ambient temperature ¿ Sterile Filtered ¿ DNase RNase and Protease Tested ¿ Concentration: 2 M Tris base and 0.05 M disodium EDTA dihydrate ¿ Product and/or packaging contains recycled/renewable content ¿ Product and/or packaging is recyclable or reduces waste ¿ Product and/or packaging is safer to human and environmental health ¿ ¿ Max Shelf Life: 36 months
Selleck Chemical LLC P62-mediated mitophagy inducer
P62-mediated mitophagy inducer (PMI) is a P62-mediated activator of mitophagy that functions independently of parkin recruitment or mitochondrial membrane potential ( m) collapse It remains effective even in cells lacking a fully functional PINK1/Parkin pathway PMI stabilizes Nrf2 and enhances p62 expression thereby activating mitophagy
Medchemexpress LLC 1,3-Propanediol, 2,2'-(1,3-propanediyldiimino)bis[2-(hydroxymethyl)- | 64431-96-5 | 99.9% | 282.33 | 1 ML
Bis-tris propane (BTP) is a water-soluble buffer substance. It can be used as a suitable buffer for polymerase chain reaction (PCR) and can enhance the stability or activity of restriction enzymes. In PCR reactions, Bis-tris propane buffer demonstrates high fidelity by stabilizing the pH of the reaction system and reducing errors introduced by DNA polymerase.
- Water-soluble buffer substance.
- Suitable buffer for polymerase chain reaction (PCR).
- Enhances the stability or activity of restriction enzymes.
- Exhibits high fidelity in PCR reactions by stabilizing pH and reducing DNA polymerase errors.
Nusep Inc NUVIEW MINI GEL 4-20 12-WELL
NuSep's nUView Tris-Glycine SDS-PAGE Precast Gel 4-20% 12well. Cassette size: 10 x 8.5 cm. Fits Mini-PRO or Bio-Rad's Mini-Protean Tetra cell Tanks. 10 gel cassettes per box. All nUView™ precast gels incorporate a unique formulation allowing protein bands to be visualized in only 2 minutes in the presence of ultraviolet (UV) light. Simply place your gel over a 250-320 nm UV trans-illuminator and watch high-resolution bands appear in just 2 minutes. With nUView™ technology you no longer have to stain and de-stain your gels – increasing protein recovery and saving you significant time while receiving improved results.
Epigentek Inc TET ASSAY BUFFER (6ML)
The TET Assay Buffer is a component of Epigenase 5mC Hydroxylase TETActivity/Inhibition Assay Kit (Colorimetric)
Cayman Chemical TrIs 4 7dIphenyl1 10phen 10mg
A luminescent probe (absorption λmax: 455 nm, luminescence λmax: 613 nm) widely used for detection and quantitation of oxygen; strongly reduced by molecular oxygen as a result of dynamic quenching; thus oxygen detection can be based on either measurement of intensity or decay time
![Research Products International Corp BIS-TRIS Propane [1,3-Bis (TRIS(hydroxymethyl)methylamino)propane], 500 Grams](https://assets.fishersci.com/TFS-Assets/CCG/product-images/VN00006095-B78000-500.0.JPG-250.jpg)
Research Products International Corp BIS-TRIS Propane [1,3-Bis (TRIS(hydroxymethyl)methylamino)propane], 500 Grams
- Substance Name: 1,3-Bis (TRIS(hydroxymethyl)methylamino)propane
- CAS Number: 64431-96-5
- Molecular Formula: C11H26N2O6
- Molecular Weight: 282.3
Abcam Kinase Assay Buffer I, 20ML
Stock solution of buffer used for assaying kinase protein.
The product is subject to the following: Abcam Restricted Use Statement
Teknova DNA Suspension Buffer, pH 8.0. 4L, Sterile, DNase/RNase tested, PCR Grade. 2 pack.
TE buffer is a salt solution that is commonly used to resuspend (or dilute) DNA and RNA. Composed of Tris base and EDTA, these molecules work together to maintain pH and prevent nucleic acids from degradation.
eMolecules 850568-79-5 | 5-Boc-amino-2-hydroxymethylphenylboronic acid, dehydrate | Combi-Blocks | MFCD06659853 | 249.070 | C12H16BNO4 | 98.000 | CC(C)(C)OC(=O)Nc1ccc2COB(O)c2c1 | 1g | 117521248
5-Boc-amino-2-hydroxymethylphenylboronic acid, dehydrate | Combi-Blocks | 850568-79-5 | MFCD06659853 | 249.070 | C12H16BNO4 | 98.000 | CC(C)(C)OC(=O)Nc1ccc2COB(O)c2c1 | 1g | 117521248
eMolecules 481681-02-1 | Ambeed | 4-Fluoro-3-(hydroxymethyl)benzeneboronic acid | 250mg | 716529157 | A129856 | MFCD08235075 | 169.95 | C7H8BFO3
Ambeed | NN3-Trimethyl-4-(4455-tetramethyl-132-dioxaborolan-2-yl)benzenesulfonamide | 1g | 719807485 | A1635881 | | MFCD29110016 | 325.230 | C15H24BNO4S
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Boston Bioproducts Inc HBS-N Buffer (10X, pH 7.4) - 4x500ml
HBS-N Buffer (10X pH 7 4) - 4x500ml (For Research Use and Further Manufacturing Only)
Boston Bioproducts Inc HBS-N Buffer (1X, pH 7.4) - 4x500ml
HBS-N Buffer (1X pH 7 4) - 4x500ml (For Research Use and Further Manufacturing Only)
Electron Microscopy Sciences D-PBS, Formula I Phosphate Buffer Saline 500 ML
All of our Dullbecco's Phosphate Buffer Saline Solutions contain Potassium Chloride, Potassium Phosphate Monobasic, Sodium Chloride, Sodium Phosphate Dibasic, and D-Glucose. Some formulations may contain additional components. Solutions are prepared 1X strength.