Life Science Buffers
Balanced salt solutions and powders used for molecular, protein, nucleic acid, and cell biology applications including cell culture, electrophoresis, ELISA, and chromatography processes; available in a variety of formulations and grades.
Buffer solutions serve to keep pH (acidity or alkalinity) nearly constant in a variety of chemical and biological applications. For example, a bicarbonate buffer helps maintain the pH of blood, a buffered saline maintains cellular contents at a consistent pH level, and buffers help maintain a narrow pH range for enzymes to function correctly.
Most biological samples used in research are kept at a pH of about 7.4. Common biological buffers used for tissue culture include Dulbeccos phosphate buffered saline (PBS), Tris base, HEPES, MOPS, PIPES, and other formulations developed for specific cell lines or applications. Most buffers used in cell culture are also DNase-, RNase-, and protease-free.
The choice of buffer for a particular biological reaction or site depends on several factors:
- Temperature
- Desired or target pH
- Buffer toxicity (to the system)
- Buffer interactions with other system components
MOLTOX Molecular Toxicology Inc Saline Solution 0.85% Nacl, 9 mL/tube, 10 tubes/box, Store at Room Temperature
MOLTOX offers a wide array of solutions and buffers for a variety of laboratory research needs. All reagents are accompanied by a GLP-compliant Formulation and Quality Control statement.
Abcam Phosphate Buffered Saline (PBS) Casein ELISA Reagent, 100ML
Phosphate Buffered Saline (PBS) Casein ELISA Reagent is a buffer designed for ELISA assay development.
The product is subject to the following: Abcam Restricted Use Statement
MOLTOX Molecular Toxicology Inc 20 mM Tris-HCL, pH 8.6, 1000 mL/bottle, Store at Room Temperature
MOLTOX manufactures a wide variety of buffers for your laboratory needs. All MOLTOX reagents are accompanied by a GLP-compliant Formulation and Quality Control statement.
Sigma Aldrich Fine Chemicals Biosciences Trizma Pre-set crystals, 100 g
BioPerformance Certified, pH 8.0, average Mw 141.8, Synonym: Tris crystals. The product is cell culture tested. It is also tested for low endotoxin levels and analyzed for the absence of nucleases and proteases.
Sigma Aldrich Fine Chemicals Biosciences BICINE BioXtra 99 titratio1KG
BICINE BioXtra 99 titratio1KG
Sigma Aldrich Fine Chemicals Biosciences BES 99.0 titration1KG
BES (N N-bis(2-hydroxyethyl)-2-aminoethanesulfonic acid) is a useful secondary standard biochemical buffer. Useful pH range for BES is 6.4 to 7.8. It is useful for diagnostic assay manufacturing industry. BES a sulfonic acid-containing cross-linking agent induces cross-linking between sulfonated polyimide chains in sulfonated polyimide membranes.
Boston Bioproducts Inc Dulbecco's Phosphate Buffered Saline, DPBS (10X) - 1L
Dulbecco s Phosphate Buffered Saline DPBS (10X) - 1L (For Research Use and Further Manufacturing Only)
Boston Bioproducts Inc Phosphate Buffered Saline, PBS (pH 7.4, Sterile-Filtered) - 20L-CBY
Phosphate Buffered Saline PBS (pH 7 4 Sterile-Filtered) - 20L-CBY (For Research Use and Further Manufacturing Only)
Boston Bioproducts Inc HEPES Buffer (0.5 M, pH 7.0, pH Adjusted with KOH) - 250ml
HEPES Buffer (0 5 M pH 7 0 pH Adjusted with KOH) - 250ml (For Research Use and Further Manufacturing Only)
Boston Bioproducts Inc 10X Dulbecco's Phosphate Buffered Saline, DPBS (no Calcium, no Magnesium) - 2x1L
Bioprocess Grade - 10X Dulbecco s Phosphate Buffered Saline DPBS (no Calcium no Magnesium) - 2x1L (For Research Use and Further Manufacturing Only)