Life Science Buffers
Balanced salt solutions and powders used for molecular, protein, nucleic acid, and cell biology applications including cell culture, electrophoresis, ELISA, and chromatography processes; available in a variety of formulations and grades.
Buffer solutions serve to keep pH (acidity or alkalinity) nearly constant in a variety of chemical and biological applications. For example, a bicarbonate buffer helps maintain the pH of blood, a buffered saline maintains cellular contents at a consistent pH level, and buffers help maintain a narrow pH range for enzymes to function correctly.
Most biological samples used in research are kept at a pH of about 7.4. Common biological buffers used for tissue culture include Dulbeccos phosphate buffered saline (PBS), Tris base, HEPES, MOPS, PIPES, and other formulations developed for specific cell lines or applications. Most buffers used in cell culture are also DNase-, RNase-, and protease-free.
The choice of buffer for a particular biological reaction or site depends on several factors:
- Temperature
- Desired or target pH
- Buffer toxicity (to the system)
- Buffer interactions with other system components
![Alkali Scientific PBS [Phosphate Buffered Saline], 1X Solution, 1.0mL Pre Filled Tubes, Sterile, 1000 Tubes per Case, 2.0mL Tube](https://assets.fishersci.com/TFS-Assets/CCG/product-images/5000661558.jpg-250.jpg)
Alkali Scientific PBS [Phosphate Buffered Saline], 1X Solution, 1.0mL Pre Filled Tubes, Sterile, 1000 Tubes per Case, 2.0mL Tube
PBS (Phosphate Buffered Saline) 1X Solution is offered in a large, ready-to-use format with 1.0mL pre-filled tubes, making it suitable for laboratory applications that require rapid, high-volume processing. With 1000 tubes per case, this product is ideal for clinical laboratories, research facilities, and biotechnological applications that perform large-scale sample analysis. PBS helps maintain osmotic pressure and pH stability, which is essential for preserving cell integrity during experiments. The pre-filled tubes offer convenience and reduce contamination risks, making them ideal for use in microbiology, molecular biology, and cell culture studies where consistent buffer conditions are required.
Sigma Aldrich Fine Chemicals Biosciences CHES >=99.0% (titration) | 103-47-9 | MFCD00003835 | 25G
CHES >=99.0% (titration) | Purity: >=99.0% (titration) | Mol Wt: 207.29 | 103-47-9 | MFCD00003835 | 25G
Sigma Aldrich Fine Chemicals Biosciences BICINE BioXtra 99 titratio1KG
BICINE BioXtra 99 titratio1KG
Sigma Aldrich Fine Chemicals Biosciences HEPES buffered saline BioUltra, for molecular biology, 2x concentrate | MFCD00006158 | 50ML
HEPES buffered saline BioUltra, for molecular biology, 2x concentrate | MFCD00006158 | 50ML
Sigma Aldrich Fine Chemicals Biosciences CAPS BioXtra, >=99% | 1135-40-6 | MFCD00003837 | 1KG
CAPS BioXtra, >=99% | Purity: >=99% | Mol Wt: 221.32 | 1135-40-6 | MFCD00003837 | 1KG
Dojindo Molecular Technologies Inc TAPS (25G), Good's buffer, CAS: 29915-38-6, pka=8.4, Active pH 7.7-9.1, made in Japan
In biological experiments, it is important to maintain the pH of the solutions used. Buffers, which are mixtures of weak acids and their appropriate conjugate bases, are commonly used in these experiments. Most biological reactions occur when the buffer is at its effective pH range of 6 to 8. Furthermore, the acids and bases used in the biological reaction should not produce chelates with metal ions. For these reasons, Dr. Good developed several aminoethane and aminopropane sulfonic acids that are now widely used for biological research and analysis. Goods buffers have the following characteristics: 1) High water-solubility. 2) Low cell membrane permeability. 3) Consistent acid-base dissociation constants. 4) Low metal chelating capability. 5) High chemical stability. 6) Low absorption spectra in UV and visible regions.
![Research Products International Corp BES [N,N-bis(2-Hydroxyethyl)-2-aminoethanesulfonic acid], 250 Grams](https://assets.fishersci.com/TFS-Assets/CCG/product-images/VN00006095-B32070-250.02.JPG-250.jpg)
Research Products International Corp BES [N,N-bis(2-Hydroxyethyl)-2-aminoethanesulfonic acid], 250 Grams
For use in preparing electrophoresis buffers. pKa (25° C) 7.1. The working pH Range in aqueous solution is 6.4-7.8
Quality Biological Inc HEPES Buffer (1 M), pH 7.3, 100 mL
HEPES is a zwitterionic organic chemical buffering agent. HEPES (1M) is used as a buffer alternative from sodium bicarbonate for cell culture and is widely used in many biochemical reactions and as a buffering agent in some cell culture media. HEPES is reportedly superior to NaHCO3 in controlling pH in tissue and organ culture.
Storage Conditions: 15 °C to 30 °C
Shipping Condition: ambient temperature
Sterile Filtered
Performance Tested
pH: 7.2-7.4
Max Shelf Life: 24 months from date of manufacture
Manufactured under an ISO 9001:2015 certified quality management system.
Product and/or packaging contains recycled/renewable content. Product and/or packaging is recyclable or reduces waste. Product and/or packaging is safer to human and environmental health.
eMolecules 2,4-Dichloro-N-(2-hydroxyethyl)benzenecarboxamide | 20656-08-0 | MFCD00141691 | 1g
Matrix Scientific | 2,4-Dichloro-N-(2-hydroxyethyl)benzenecarboxamide | 1g | 389721058 | 45569 | 95.000 | 20656-08-0 | MFCD00141691 | 234.080 | C9H9Cl2NO2
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![Alkali Scientific PIPES Sesquisodium Salt [Piperazine N,N' bis (2 ethanesulfonic acid) sesquisodium salt], 250 G](https://assets.fishersci.com/TFS-Assets/CCG/product-images/5000661552.jpg-250.jpg)
Alkali Scientific PIPES Sesquisodium Salt [Piperazine N,N' bis (2 ethanesulfonic acid) sesquisodium salt], 250 G
PIPES Sesquisodium Salt (Piperazine N,N' bis(2 ethanesulfonic acid) sesquisodium salt) is a high-purity reagent provided in a 250G pack. It is commonly used as a buffering agent in biochemical and laboratory applications due to its stability at a wide range of pH levels. PIPES is primarily used in the preparation of buffers for biological assays and research, as it does not interfere with biological systems. The sesquisodium salt form helps maintain the pH in both acidic and basic environments. This reagent is suitable for cell culture, enzyme assays, and other biochemical studies where precise pH control is essential. It is also utilized in electrophoresis, chromatography, and immunological testing.
IBA LifeSciences Buffer XT-R - Strep-TactinXT regeneration buffer; 250 ml
Buffer XT-R is a ready-to-use Strep-TactinXT regeneration buffer containing magnesium chloride for competitive displacement of biotin from the modified biotin-binding pocket of Strep-TactinXT after the elution of Strep-tagII or Twin-Strep-tag fusion proteins. Please note that Buffer XT-R is not applicable for Strep-Tactin.
Boston Bioproducts Inc Phosphate Buffered Saline, PBS (pH 7.4, Sterile-Filtered) - 500ml
Phosphate Buffered Saline (PBS) is a versatile buffer solution widely used in biological research due to its isotonic and non-toxic nature to cells. With a pH range of 7.0 to 7.4, PBS mimics the osmolarity and ion concentrations of the human body, making it ideal for a variety of applications including protein purification, as wash buffer in Western blot and immunoassays, sample dilution, and as a transport medium for tissue samples as well as RNA viruses.
Sigma Aldrich Fine Chemicals Biosciences Tris(hydroxymethyl)aminomethane ACS reagent, >=99.8% | 77-86-1 | MFCD00004679 | 100G
Tris(hydroxymethyl)aminomethane ACS reagent, >=99.8% | Purity: >=99.8%; 99.8-100.1% | Mol Wt: 121.14 | 77-86-1 | MFCD00004679 | 100G
Sigma Aldrich Fine Chemicals Biosciences HEPES 99.5 (titration), 7365-45-9, MFCD00006158
HEPES 99.5 (titration)