Life Science Buffers
Balanced salt solutions and powders used for molecular, protein, nucleic acid, and cell biology applications including cell culture, electrophoresis, ELISA, and chromatography processes; available in a variety of formulations and grades.
Buffer solutions serve to keep pH (acidity or alkalinity) nearly constant in a variety of chemical and biological applications. For example, a bicarbonate buffer helps maintain the pH of blood, a buffered saline maintains cellular contents at a consistent pH level, and buffers help maintain a narrow pH range for enzymes to function correctly.
Most biological samples used in research are kept at a pH of about 7.4. Common biological buffers used for tissue culture include Dulbeccos phosphate buffered saline (PBS), Tris base, HEPES, MOPS, PIPES, and other formulations developed for specific cell lines or applications. Most buffers used in cell culture are also DNase-, RNase-, and protease-free.
The choice of buffer for a particular biological reaction or site depends on several factors:
- Temperature
- Desired or target pH
- Buffer toxicity (to the system)
- Buffer interactions with other system components
Sigma Aldrich Fine Chemicals Biosciences MOPS solution BioPerformance Certified, 1 M, suitable for cell culture | MFCD00006181 | 100ML
MOPS solution BioPerformance Certified, 1 M, suitable for cell culture | MFCD00006181 | 100ML
Sigma Aldrich Fine Chemicals Biosciences Dulbeccos Modified Eagles500ML
This modification has been shown to improve the stability of the medium.
Sigma Aldrich Fine Chemicals Biosciences Dulbeccos Phosphate Bufer500ML
Dulbecco Phosphate Buffered Saline (DPBS) is a balanced salt SOLUTIONon.
Sigma Aldrich Fine Chemicals Biosciences Dulbeccos Phosphate Bufer500ML
Dulbeccos Phosphate Bufer500ML
Sigma Aldrich Fine Chemicals Biosciences Trizma Pre-set crystals, 100 g
BioPerformance Certified, pH 8.0, average Mw 141.8, Synonym: Tris crystals. The product is cell culture tested. It is also tested for low endotoxin levels and analyzed for the absence of nucleases and proteases.
Teknova 2M Tris-HCl, pH 7.5 Solution. 1000mL, Sterile. 2 pack.
Tris (tris(hydroxymethyl)aminomethane) is used as a component ofbuffer solutions, especially for solutions ofnucleic acids. The useful buffer range for tris (pH 7–9) is similar to the pH of most living organisms. This, and its low cost, make tris one of the most common buffers in the biology/biochemistry laboratory.
Boston Bioproducts Inc Phosphate Buffered Saline-Tween 10X, 4 x 1 L bottles, 0.5% Tween-20, pH 7.4, liquid
Phosphate Buffered Saline-Tween 10X, 4 x 1 L bottles, 0.5% Tween-20, pH 7.4, liquid
Medchemexpress LLC Pipes dipotassium | 108321-27-3 | MFCD00069751 | 98.0% | 378.55 g/mol | C8H16K2N2O6S2 | 50 G
PIPES (dipotassium) is the dipotassium salt of the zwitterionic buffering agent PIPES (1,4-piperazinediethanesulfonic acid). Supplied as a high-purity dry powder, it is used to prepare buffering solutions for biochemical and cell biology research where stable pH is required.
- High purity: 98.0%.
- Dipotassium salt form improves aqueous solubility.
- Suitable for preparing pH-stable buffer solutions in biological assays.
- Provided as a 50 g reagent pack for laboratory use.
- Documentation available: data sheet, SDS, and COA.
Medchemexpress LLC Pipes dipotassium | 108321-27-3 | MFCD00069751 | 98.0% | 378.55 | C8H16K2N2O6S2 | 100 G
PIPES (dipotassium) is the dipotassium salt of the zwitterionic buffering agent PIPES, provided as a white to off-white solid. It offers stable buffering near physiological pH with low metal ion binding, making it suitable for biochemical and cell biology applications such as assay buffers, electrophoresis, and sample preparation.
Electron Microscopy Sciences Phosphate Buffered Saline, 2X 1 GL
PBS is commonly used in biochemistry. It is a salty solution containing sodium chloride, potassium chloride, sodium phosphate and potassium phosphate. PBS is isotonic and non-toxic to cells. 1X PBS final concentration is 8.0 g/L NaCl, 0.2 g/L KCl, 1.42 g/L Na2HPO4, 0.24 g/L KH2PO4 and pH 7.4.
Electron Microscopy Sciences Tris Buffered Saline, pH 7.4 250 ML
C₄H₁₁NO₃ F.W. 121.14
Our buffers are ideal for maintaining a constant pH during fixation and have an ionic constitution to prevent the extraction of cellular components or the deposition of precipitates of fixative chemicals. The buffers are prepared fresh for each order and conveniently packaged.
pH: 7.4
Electron Microscopy Sciences Tris HCL Buffer, pH range 7.2-9.0 250 ML
Please specify pH when ordering.
pH: 7.2-9.0