Life Science Buffers
Balanced salt solutions and powders used for molecular, protein, nucleic acid, and cell biology applications including cell culture, electrophoresis, ELISA, and chromatography processes; available in a variety of formulations and grades.
Buffer solutions serve to keep pH (acidity or alkalinity) nearly constant in a variety of chemical and biological applications. For example, a bicarbonate buffer helps maintain the pH of blood, a buffered saline maintains cellular contents at a consistent pH level, and buffers help maintain a narrow pH range for enzymes to function correctly.
Most biological samples used in research are kept at a pH of about 7.4. Common biological buffers used for tissue culture include Dulbeccos phosphate buffered saline (PBS), Tris base, HEPES, MOPS, PIPES, and other formulations developed for specific cell lines or applications. Most buffers used in cell culture are also DNase-, RNase-, and protease-free.
The choice of buffer for a particular biological reaction or site depends on several factors:
- Temperature
- Desired or target pH
- Buffer toxicity (to the system)
- Buffer interactions with other system components
Biotang Inc HEPES Buffer 0.5M, pH 8.0, 100ml, 500 mM HEPES (4-(2-Hydroxyethyl)piperazine-1-ethanesulfonic acid), pH 8.0 +/- 0.15. BB-2080
HEPES Buffer 0.5M, pH 8.0, 100ml, 500 mM HEPES (4-(2-Hydroxyethyl)piperazine-1-ethanesulfonic acid), pH 8.0 +/- 0.15. BB-2080.
Bioworld BES (2-(Bis(2-hydroxyethyl) amino) ethanesulfonic acid) Buffer, 0.5M, pH 7.0, 250 mL
Synonym:N,N-Bis(2-hydroxyethyl)-2-aminoethanesulfonic acid sodium salt, BES sodium saltBelongs to the class of Good buffers, as described in the research of Dr. Norman Good et al. (1966), optimized for molecular biology and biochemistry applications. The characteristics of a Good′s buffer include: pKa between 6.0 and 8.0, high solubility, non toxic, limited effect on biochemical reactions, very low absorbance between 240-700 nm, enzymatic and hydrolytic stability, minimal changes due to temperature and concentration, limited effects due to ionic or salt composition of the solution, limited interaction with mineral cations and limited permeability of biological membranes.Technical Information :Useful pH range :6.4 - 7.8pKa (25 °C) :7.1 (Free acid)Warning:For laboratory use only.Not fit for agricultural, household, clinical use or human consumption.
Medchemexpress LLC Rasagiline-13C3 mesylate | 1391052-18-8 | 270.32 | 1 MG
Rasagiline-13C3 mesylate is a deuterium-labeled Rasagiline mesylate, which acts as a highly potent, selective, and irreversible mitochondrial monoamine oxidase (MAO) inhibitor. It exhibits IC50s of 4.43 nM and 412 nM for rat brain MAO B and A activity, respectively.
- Used as a tracer.
- Internal standard for quantitative analysis by NMR, GC-MS, or LC-MS.
- Incorporates stable heavy isotopes into drug molecules for quantitation.
- Deuteration can affect the pharmacokinetic and metabolic profiles of drugs.
![Alkali Scientific PBS 1X Solution [Phosphate Buffered Saline 1X Sterile Solution], 500 Milliliters](https://assets.fishersci.com/TFS-Assets/CCG/product-images/5000660133.jpg-250.jpg)
Alkali Scientific PBS 1X Solution [Phosphate Buffered Saline 1X Sterile Solution], 500 Milliliters
PBS (Phosphate Buffered Saline) 1X Solution is a sterile, isotonic buffer solution used in a variety of biological applications. The 500 mL volume is convenient for smaller-scale applications, including cell culture, molecular biology, and immunology experiments. PBS helps maintain the osmotic balance and pH of biological systems, preventing cell lysis or shrinkage. It is frequently used to wash cells, dilute samples, and prepare reagents for biochemical assays. PBS is an essential component in maintaining physiological conditions in in vitro studies and ensuring that cells, tissues, and biomolecules remain stable during handling and experimental procedures.
Teknova 1X PBS Solution, pH 7.4. 500mL, Sterile 8 pack.
1X PBS Solution, pH 7.4. 500mL, Sterile 8 pack.
Teknova 10X PBS-MK, 1000mL, Sterile.
Phosphate-buffered saline (PBS) is a salt solution that is commonly used in physiological and biochemical experiments. It can be used to maintain pH neutrality or to buffer against changes in pH in order to provide optimal conditions for cellular growth and function. The buffering range is between pH 6.8 and 7.4.
Research Products International Corp MES, Anhydrous, 100 Grams
- CAS Number: 4432-31-9
- Chemical Formula: C6H13NO4S
- Molecular Weight: 195.24 g/mol
Research Products International Corp PIPES FREE ACID ULTRAPPURE, 50GM
Heavy metals: <10 ppm
pka (25°C): 6.8
The working pH range in aqueous solution is 6.5-7.5
Crescent Chemical Co Inc MORPHOLINOETHANE SULFONIC ACID
Morpholinoethane sulfonic acid analytical grade pKa 20 = 6.15. Buffering substance for biochemistry and molecular biology (1). Physical constants (2). Size - 100G Storage Conditions - +15 °C TO +30 °C Catalog Number - 29834.02 CAS 4432-31-9
Bioworld BES, 1M, pH 7.0, 200 mL
BES bufferAlso known as N,N-Bis(hydroxyethyl)-2aminoethanesulfonic acid.Technical Information:pKA (25 C): 7.1Heavy Metals: <0.0005%. Appearance: LiquidBoiling Point : 404.34 °C (Predicted)Density : 1.44 g/cm3 (Predicted)Melting Point: 152-154 °CPH Value: 6.4-7.8
Crescent Chemical Co Inc MORPHOLINOETHANE SULFONIC ACID
Morpholinoethane sulfonic acid analytical grade pKa 20 = 6.15. Buffering substance for biochemistry and molecular biology (1). Physical constants (2). Size - 1KG Storage Conditions - +15 °C TO +30 °C Catalog Number - 29834.03 CAS 4432-31-9
Bioworld ADA (N-(2-acetamido)iminodiacetic acid) Buffer, 0.2M, pH 7.5, 250 mL
Synonym:ADA-Na,N-(2-Acetamido)iminodiacetic acid monosodium salt,N-(Carbamoylmethyl)iminodiacetic acid monosodium saltA zwitterionic buffer used in biological applications including protein isolation and characterizationWarning: For laboratory use only.Not fit for agricultural, household, clinical use or human consumption. Appearance: LiquidPH Value: 7.5
Dojindo Molecular Technologies Inc TES (100G), Good's buffer, CAS: 7365-44-8, pka=7.5, Active pH 6.8-8.2, made in Japan
In biological experiments, it is important to maintain the pH of the solutions used. Buffers, which are mixtures of weak acids and their appropriate conjugate bases, are commonly used in these experiments. Most biological reactions occur when the buffer is at its effective pH range of 6 to 8. Furthermore, the acids and bases used in the biological reaction should not produce chelates with metal ions. For these reasons, Dr. Good developed several aminoethane and aminopropane sulfonic acids that are now widely used for biological research and analysis. Goods buffers have the following characteristics: 1) High water-solubility. 2) Low cell membrane permeability. 3) Consistent acid-base dissociation constants. 4) Low metal chelating capability. 5) High chemical stability. 6) Low absorption spectra in UV and visible regions.