Life Science Buffers
Balanced salt solutions and powders used for molecular, protein, nucleic acid, and cell biology applications including cell culture, electrophoresis, ELISA, and chromatography processes; available in a variety of formulations and grades.
Buffer solutions serve to keep pH (acidity or alkalinity) nearly constant in a variety of chemical and biological applications. For example, a bicarbonate buffer helps maintain the pH of blood, a buffered saline maintains cellular contents at a consistent pH level, and buffers help maintain a narrow pH range for enzymes to function correctly.
Most biological samples used in research are kept at a pH of about 7.4. Common biological buffers used for tissue culture include Dulbeccos phosphate buffered saline (PBS), Tris base, HEPES, MOPS, PIPES, and other formulations developed for specific cell lines or applications. Most buffers used in cell culture are also DNase-, RNase-, and protease-free.
The choice of buffer for a particular biological reaction or site depends on several factors:
- Temperature
- Desired or target pH
- Buffer toxicity (to the system)
- Buffer interactions with other system components
Sigma Aldrich Fine Chemicals Biosciences MES sodium salt | 71119-23-8 | MFCD00065473 | 100g
MES sodium salt | Purity: 99% | MW: 217.22 | 71119-23-8 | MFCD00065473 | 100g
Sigma Aldrich Fine Chemicals Biosciences PIPES disodium salt >=99% (titration) | 76836-02-7 | MFCD00064349 | 1KG
PIPES disodium salt >=99% (titration) | Purity: >=99% (titration) | Mol Wt: 346.33 | 76836-02-7 | MFCD00064349 | 1KG
Bioworld MODIFIED DULBECCO S PBS PACKS
Modified Dulbecco\'s PBS Packs are pouches of dry-blend powder that are each sufficient to make 500mL of physiological Na- and K-phosphate buffered saline (D-PBS) for ELISA and Western blot diluents and wash buffers. Dry-Blend Packs of m...
Sigma Aldrich Fine Chemicals Biosciences SIGMA ALDRICH FINE CHEMICALS BIOSCIENCES
NC4040864 BUFFERED AQUEOUS GLYCEROL SOLU
Bioworld PBS/Fish Gelatin (10X), 500 mL
PBS/Fish Gelatin (1x) Phosphate Buffered Saline/fish gelatin 0.5% blocks, diluents, and coating solutions.Technical Information 1x: Fish Gelatin 0.5%PBS (1x)pH range: 7.4 (+/- 0.1)
Miltenyi Biotec, Inc. CLINIMACS PBS/EDTA BUFFER 2 X
5000277983 CLINIMACS PBS/EDTA BUFFER 2 X
Biotang Inc Phosphate-Buffered Saline (PBS, 10X)For Western Blot Washing, 1000 ml
Phosphate-Buffered Saline (PBS, 10X)For Western Blot Washing, 1000 ml.
Apexbio Technology LLC Annexin V-PE/DAPI Apoptosis Kit 20tests
Annexin V-PE/DAPI Apoptosis Kit enables differentiation between early apoptotic late apoptotic and dead cells Annexin V is a Ca2 -dependent phospholipid-binding protein that specifically binds to phosphatidylserine (PS) PS is normally located on the inner leaflet of the cell membrane phospholipid bilayer However during the early stages of apoptosis PS moves from the inner surface to the outer surface of the cell membrane becoming exposed to the extracellular environment Labeled with a fluorescence dye such as PE Annexcin V is commonly used as a fluorescent probe to detect apoptosis through flow cytometry or fluorescence microscopy within 15-30 minutes Additionally this kit includes the nuclear stain DAPI which specifically binds to double-stranded DNA to emit blue fluorescence Normally DAPI cannot penetrate intact cell membranes In late apoptotic and dead cells DAPI can cross the compromised membrane to stain the nucleus
Apexbio Technology LLC One-step TUNEL Cy3 Apoptosis Detection Kit 50rxns
One-step TUNEL Cy3 Apoptosis Detection Kit can be used to detect nuclear DNA fragmentation occurring in tissues or cells during apoptosis Cellular DNA endonucleases are activated during apoptosis which cleave genomic DNA between nucleosomes yielding DNA fragments of approximately 180 - 200 bp or their multiples The resulting DNA fragments have exposed 3 -OH ends which can be labeled with a fluorescein-modified dUTP under the action of Terminal Deoxynucleotidyl Transferase (TdT) These labeled DNA fragments can then be detected using fluorescence microscopy or flow cytometry (Ex/Em) of 550 nm/570 nm This detection kit has a wide range of applications and can be used to assay apoptosis in frozen sections or paraffin-embedded tissue sections as well as cultured adherent or suspended cells
Apexbio Technology LLC TUNEL Apoptosis Detection Kit (DAB) 20rxns
The TUNEL Apoptosis Detection Kit (DAB) is designed to detect nuclear DNA fragmentation occurring during apoptosis in tissues or cells During apoptosis endonucleases within cells become activated and cleave genomic DNA between nucleosomes resulting in DNA fragments of approximately 180-200 bp or multiples thereof The fragmented DNA generates free 3 -OH termini at which terminal deoxynucleotidyl transferase (TdT) incorporates biotin-labeled dUTP Subsequently these labeled termini bind to horseradish peroxidase (HRP)-conjugated streptavidin (Streptavidin-HRP) followed by chromogenic visualization using DAB This kit allows direct observation using an ordinary optical microscope and is convenient for use It is applicable to a wide range of sample types including frozen sections paraffin-embedded sections adherent cell cultures and suspended cells making it versatile for apoptosis detection
![Alkali Scientific PBS [Phosphate Buffered Saline] 200mL Tablets, 500 Tablets per Bottle](https://assets.fishersci.com/TFS-Assets/CCG/product-images/5000660121.jpg-250.jpg)
Alkali Scientific PBS [Phosphate Buffered Saline] 200mL Tablets, 500 Tablets per Bottle
PBS [Phosphate Buffered Saline] 200mL Tablets are designed for laboratories that need to prepare larger volumes of PBS quickly and accurately. Each tablet dissolves easily in water to create 200 mL of phosphate-buffered saline, a standard buffer used in many biological and biochemical applications. The 500-tablet bottle is perfect for high-throughput labs that require a consistent and reliable buffer solution. These tablets ensure that the PBS solution maintains the correct pH and osmolarity, which is essential for applications such as cell culture, protein assays, and molecular biology experiments. The tablets are convenient, cost-effective, and reduce the chance of errors in solution preparation.
ILEX LIFE SCIENCES LLC EDTA 1P P10-026100 6 X 100 ML
PAN-Biotech EDTA 1% in PBS, w/o: Ca and Mg (6 x 100 ml). DESCRIPTION: PAN-Biotech EDTA 1% in PBS, w/o: Ca and Mg. Ethylenediaminetetraacetic acid (EDTA), a chelating agent, is often used to remove calcium from cell washing and suspension media. EDTA can reduce clumping of cells grown in liquid suspension. It is also used for gentle detachment of certain adherent cells for passaging. EDTA is often added to trypsin solutions to enhance enzymatic activity by neutralizing calcium and magnesium ions that enhance cell-to-cell adhesion and obscure the peptide bonds on which trypsin acts. LIQUID/POWDER: Liquid. PHENOL RED: Without Phenol Red. Ca/Mg CONTENT: Without Ca and Mg. EDTA: 1.00% EDTA. STERILE: Yes. STORAGE TEMP: +2-8°C. MANUFACTURER: PAN-Biotech GmbH (Germany).