Life Science Buffers
Balanced salt solutions and powders used for molecular, protein, nucleic acid, and cell biology applications including cell culture, electrophoresis, ELISA, and chromatography processes; available in a variety of formulations and grades.
Buffer solutions serve to keep pH (acidity or alkalinity) nearly constant in a variety of chemical and biological applications. For example, a bicarbonate buffer helps maintain the pH of blood, a buffered saline maintains cellular contents at a consistent pH level, and buffers help maintain a narrow pH range for enzymes to function correctly.
Most biological samples used in research are kept at a pH of about 7.4. Common biological buffers used for tissue culture include Dulbeccos phosphate buffered saline (PBS), Tris base, HEPES, MOPS, PIPES, and other formulations developed for specific cell lines or applications. Most buffers used in cell culture are also DNase-, RNase-, and protease-free.
The choice of buffer for a particular biological reaction or site depends on several factors:
- Temperature
- Desired or target pH
- Buffer toxicity (to the system)
- Buffer interactions with other system components
Quality Biological Inc PBS pH 7.2, CS 10 X 500 mL
PBS (phosphate buffered saline) is a balanced salt solution ¿ Used for a variety biological and cell culture applications, such as washing cells before dissociation, transporting cells or tissue, diluting cells for counting, and preparing reagents ¿ Endotoxin and Mycoplasma tested ¿ Also useful in immunological applications, such as Western Blotting ¿ Storage Condition: 15¿C ¿ 30¿C ¿ Shipping Condition: ambient temperature ¿ Sterile Filtered ¿ Endotoxin and Mycoplasma Tested ¿ pH: 7.1 -7.3 ¿ Osmolality: 300¿5% mOsm/Kg H2O ¿ Product and/or packaging contains recycled/renewable content Product and/or packaging is recyclable or reduces waste ¿ Product and/or packaging is safer to human and environmental health ¿ UNSPSC: 12161700 ¿ ¿ Max Shelf Life: 24 months
Boston Bioproducts Inc HEPES Buffer (1 M, pH 7.0) - 250ml
The HEPES [4-(2-hydroxyethyl)-1-piperazineethanesulfonic acid)] Buffer is a versatile Good's zwitterionic buffering agent widely used in various applications in biochemistry and molecular biology. It maintains a stable pH in the range of 6.8 to 8.2, making it ideal for various applications at physiological pH, including cell culture, protein purification, isoelectric focusing, enzyme studies, bioprocessing, and live cell imaging.
Medchemexpress LLC 1H-1,4,7-triazonine-1,4,7-triacetic acid, α1-(2-carboxyethyl)hexahydro-, tris(t-Bu) ester | 1190101-34-8 | MFCD29083820 | 99.8% | 543.69 g/mol | C27H49N3O8 | 100 MG
NODAGA-tris(t-Bu ester) is a tri-tert-butyl ester-protected NODAGA chelator intended for conjugation to biomolecules and subsequent radiolabeling for positron emission tomography (PET) studies. It provides a protected, bifunctional scaffold that facilitates attachment to peptides, proteins, and antibodies and enables stable chelation of radiometals after deprotection.
- Protected NODAGA chelator for conjugation to biomolecules.
- Suitable for labeling peptides, proteins, and antibodies.
- Designed for radiolabeling workflows for PET imaging.
- High purity (99.8%) with RP-HPLC documentation available.
- Available as solid and as DMSO solutions for convenience.
- Molecular formula C27H49N3O8; molecular weight 543.69 g/mol.
Medchemexpress LLC 1H-1,4,7-triazonine-1,4,7-triacetic acid, α1-(2-carboxyethyl)hexahydro-, tris(tert-butyl) ester | 1190101-34-8 | MFCD29083820 | 99.8% | 543.69 g/mol | C27H49N3O8 | 50 MG
NODAGA-tris(t-Bu ester) is a tert-butyl-protected bifunctional chelator precursor used for covalent attachment to biomolecules. It is designed to conjugate to peptides, proteins, or antibodies; after deprotection the chelator can be radiolabeled for PET imaging studies. The reagent is supplied as a high-purity research chemical suitable for labeling workflows and analytical characterization.
- Tert-butyl protected chelator precursor suitable for conjugation to peptides and antibodies.
- Compatible with deprotection and subsequent radiolabeling for PET imaging.
- High purity as measured by HPLC, appropriate for research use.
- Soluble in DMSO for formulation and conjugation workflows.
- Molecular weight 543.69 g/mol; chemical formula C27H49N3O8.
Sigma Aldrich Fine Chemicals Biosciences Tris-Glycine-SDS Buffer 101L
Tris-glycine-SDS (TGS) running buffer is the most commonly used buffer for sodium dodecyl sulfate-polyacrylamide gel electrophoresis (SDS-PAGE) of proteins. TGS is usually used for both anode and cathode buffer. Recommended running conditions is 150 volts for mini vertical gel electrophoresis units.
Sigma Aldrich Fine Chemicals Biosciences Dulbeccos Phosphate Buffer
DULBECCOS Dulbeccos Phosphate Buffer
Sigma Aldrich Fine Chemicals Biosciences PIPES >=99% (titration) | 5625-37-6 | MFCD00006159 | 25G
PIPES >=99% (titration) | Purity: >=99% (titration) | Mol Wt: 302.37 | 5625-37-6 | MFCD00006159 | 25G
Medchemexpress LLC 4-Morpholineethanesulfonic acid, hydrate | 1266615-59-1 | 99.9% | 25 G
4-Morpholineethanesulfonic acid, hydrate | 1266615-59-1 | 99.9% | 25 G
STEMCELL Technologies HEPES Buffer Solution (1 M), Size: 100 mL
HEPES Buffer Solution is a biological buffer used in cell culture media. Selection of suitable nutrient medium is dependent on type of cell, conditions of culture, and degree of chemical definition required for the cell culture application.
Boston Bioproducts Inc HEPES Buffer (1 M, pH 7.6, pH Adjusted with KOH) - 250ml
The HEPES [4-(2-hydroxyethyl)-1-piperazineethanesulfonic acid)] Buffer is a versatile Good's zwitterionic buffering agent widely used in various applications in biochemistry and molecular biology. It maintains a stable pH in the range of 6.8 to 8.2, making it ideal for various applications at physiological pH, including cell culture, protein purification, isoelectric focusing, enzyme studies, bioprocessing, and live cell imaging. This buffer is adjusted with KOH.
Sigma Aldrich Fine Chemicals Biosciences BICINE BioXtra 99 titratio250G
BICINE BioXtra 99 titratio250G
Sigma Aldrich Fine Chemicals Biosciences PIPES BioPerformance Certi500G
PIPES BioPerformance Certi500G
Sigma Aldrich Fine Chemicals Biosciences HEPES BioXtra pH 5.0-6.5 150G
HEPES has been described as one of the best all-purpose buffers available for biological research. At biological pH the molecule is zwitterionic and is effective as a buffer at pH 6.8 to 8.2. HEPES has been used in a wide variety of applications including tissue culture. It is commonly used to buffer cell culture media in air. HEPES finds its usage in in vitro experiments on Mg.
Sigma Aldrich Fine Chemicals Biosciences HEPES buffer solution 1 M in H2O | 7365-45-9 | MFCD00006158 | 100ml
HEPES buffer solution 1 M in H2O | Mol Wt: | 7365-45-9 | MFCD00006158 | 100ml
Sigma Aldrich Fine Chemicals Biosciences Hepes|7365-45-9 | MFCD00006158 | 100g
Hepes | Purity: 99.5% (titration) | MW: 238.3 | 7365-45-9 | MFCD00006158 | 100g