Life Science Buffers
Balanced salt solutions and powders used for molecular, protein, nucleic acid, and cell biology applications including cell culture, electrophoresis, ELISA, and chromatography processes; available in a variety of formulations and grades.
Buffer solutions serve to keep pH (acidity or alkalinity) nearly constant in a variety of chemical and biological applications. For example, a bicarbonate buffer helps maintain the pH of blood, a buffered saline maintains cellular contents at a consistent pH level, and buffers help maintain a narrow pH range for enzymes to function correctly.
Most biological samples used in research are kept at a pH of about 7.4. Common biological buffers used for tissue culture include Dulbeccos phosphate buffered saline (PBS), Tris base, HEPES, MOPS, PIPES, and other formulations developed for specific cell lines or applications. Most buffers used in cell culture are also DNase-, RNase-, and protease-free.
The choice of buffer for a particular biological reaction or site depends on several factors:
- Temperature
- Desired or target pH
- Buffer toxicity (to the system)
- Buffer interactions with other system components
Sigma Aldrich Fine Chemicals Biosciences TAPS sodium salt 99 titrat500G
TAPS sodium salt 99 titrat500G
Teknova 50mM MES 1M Sodium Chloride
50mM MES, 1M Sodium Chloride, pH 6.0, 1000mL, Endotoxin Tested, Sterile. 8 pack.
Sigma Aldrich Fine Chemicals Biosciences TAPS BioXtra 99.5 titratio100G
TAPS9 TAPS BioXtra 99.5 titratio100G
Sigma Aldrich Fine Chemicals Biosciences Dulbeccos Modified Eagles1L
This DMEM-Hi glucose medium is a 1x complete medium with no added factors (common modifications) such as L-alanyl-L-glutamine HEPES or sodium pyruvate. It differs from the original DMEM-Hi formulation wherein pyridoxine is substituted for pyridoxal. Pyridoxal is an unstable component of media. This medium requires supplementation with L-glutamine or L-alanyl-L-glutamine.
Scytek Laboratories ScyTek Phosphate Buffered Saline (25x), 7.6 pH, 1000ml
ScyTek Phosphate Buffered Saline (25x) pH of 7.6 is an optimal formulation of pH stabilizers and salts designed to effectively remove excess material from the microtiter plate wells without disrupting the ELISA binding reaction. By maintaining the proper buffering environment, unbound components can be washed away without suppressing antigen-antibody binding interactions, thereby reducing nonspecific background and increasing the specific signal. Our Wash buffers do not contain hazardous preservatives such as Azide or Mercury that may interfere with antibody-antigen binding interactions. For your convenience wash buffer is offered in a wide variety of formulations to meet the needs of your specific ELISA application. This product can be customized to meet the specific needs of your assay. Inquire about custom vialing, labeling, kit assembly and drop shipping.
Ethos Biosciences Dilurex 1X PBS Buffer, 100ml, Ethos
Dilurex 1X PBS is a ready-to-use working concentration of pH 7.4 phosphate buffered saline. Skip the tedium of weighing powders and stocking raw materials that can expire. Our PBS is convenient, ready to use, and will save you hours in the lab, letting you focus on breakthroughs rather than buffer prep. Thousands of researchers trust Ethos Biosciences 1X PBS for its proven track record in top-tier publications and protocols - upgrade your productivity today.
Lonza Walkersville DPBS Powder 10L
Lonza s 15-512 powder is a skeleton DPBS powder without calcium and magnesium to reconstitute all liquid DPBS formulations This DPBS powder without calcium and magnesium is available in 10 50 and 1000 containers This means these containers can make 10 50 or 1000L of liquid medium when dissolved in water This BioWhittaker Cell Culture Powder is produced according to cGMP and labelled For Further Manufacturing (FFM) GMP Products are intended for research or further manufacturing use only THIS PRODUCT IS NOT INTENDED FOR DIRECT THERAPEUTIC USE IN HUMANS
Teknova 1X PBS Solution plus 0.05M Sodium Chloride, pH 2.6. 1000mL, Sterile. 2 pack.
Phosphate-buffered saline (PBS) is a salt solution that is commonly used in physiological and biochemical experiments. It can be used to maintain pH neutrality or to buffer against changes in pH in order to provide optimal conditions for cellular growth and function. The buffering range is between pH 6.8 and 7.4.
Apexbio Technology LLC Annexin V-HF647/DAPI Apoptosis Kit 20assays
Annexin V is a Ca2 -dependent phospholipid-binding protein that specifically binds to phosphatidylserine (PS) Normally PS is localized on the inner leaflet of the cell membrane lipid bilayer During the early stages of apoptosis PS translocates from the inner leaflet to the outer surface of the cell membrane exposing it to the extracellular environment Due to Annexin V s high affinity for PS fluorescently labeled Annexin V (HF647) can be used as a fluorescent probe to detect apoptotic cells through flow cytometry or fluorescence microscopy with staining taking only 10-20 minutes DAPI specifically binds double-stranded DNA producing blue fluorescence and normally cannot penetrate intact cell membranes However during late apoptosis or in dead cells DAPI can pass through compromised membranes to stain the nucleus Concurrent staining with Annexin V-HF647 and DAPI allows differentiation of cells at different stages of apoptosis and identification of dead cells
Alkali Scientific CellPro™ PBS Buffer, 1X, p.H 7.2, 1L, 1 Liter, 10/Case
CellPro™ PBS Buffer, 1X pH 7.2, 1 Liter Phosphate-buffered saline (PBS) is a balanced salt solution that is used for a variety of cell culture applications, such as washing cells before dissociation, transporting cells or tissue samples, diluting cells for counting, and preparing reagents. PBS is formulated without calcium and magnesium for rinsing chelators from the culture before cell dissociation. Concentrated forms of Gibco™ PBS may require pH adjustment at the time of dilution.
Apexbio Technology LLC EdU Flow Cytometry Assay Kits (488) 100 tests
EdU Flow Cytometry Assay Kits (488) provides a novel method based on click chemistry specifically CuAAC (copper-catalyzed azide-alkyne cycloaddition) enabling direct measurement of DNA synthesis during the S-phase of the cell cycle The thymidine analog EdU (5-ethynyl-2 -deoxyuridine) can be incorporated directly into DNA strands during DNA synthesis The alkyne group on EdU a biologically inert moiety undergoes highly selective reaction with azide-functionalized dyes via the CuAAC reaction forming a stable 1 2 3-triazole product The unique biological groups on EdU and 6-FAM Azide allow fluorescent labeling of proliferating cell DNA after conjugation enabling imaging with low background and high detection sensitivity The EdU Flow Cytometry Assay Kits (488) use 6-FAM azide to label the DNA of proliferating cells following its reaction with EdU Samples labeled in this manner can be analyzed via flow cytometry (EXC/EMI 496/516 nm)) with green fluorescence