Life Science Buffers
Balanced salt solutions and powders used for molecular, protein, nucleic acid, and cell biology applications including cell culture, electrophoresis, ELISA, and chromatography processes; available in a variety of formulations and grades.
Buffer solutions serve to keep pH (acidity or alkalinity) nearly constant in a variety of chemical and biological applications. For example, a bicarbonate buffer helps maintain the pH of blood, a buffered saline maintains cellular contents at a consistent pH level, and buffers help maintain a narrow pH range for enzymes to function correctly.
Most biological samples used in research are kept at a pH of about 7.4. Common biological buffers used for tissue culture include Dulbeccos phosphate buffered saline (PBS), Tris base, HEPES, MOPS, PIPES, and other formulations developed for specific cell lines or applications. Most buffers used in cell culture are also DNase-, RNase-, and protease-free.
The choice of buffer for a particular biological reaction or site depends on several factors:
- Temperature
- Desired or target pH
- Buffer toxicity (to the system)
- Buffer interactions with other system components
Boston Bioproducts Inc Tris Buffered Saline, TBS (10X, pH 7.4) - 4L
The Tris Buffered Saline (TBS) is an isotonic non-toxic buffer solution used commonly in biochemical immunoassay as a wash buffer. Tris maintains a stable pH range typically between 7.4 to 8.0 while NaCl regulates osmotic balance via its elected concentration. Tris maintains a stable pH range typically between 7.4 to 8.0 while NaCl regulates osmotic balance via its elected concentration. This TBS Solution is formulated at a 10X concentration.
![LGC GENOMICS LLC (Lucigen) TE Buffer, 70 mLStandard buffer for storing nucleic acids (10 mM Tris-HCl [pH 7.5], 1 mM EDTA). To be used with the MasterPure Kits and any other nucleic acid extraction/purification kits.](https://assets.fishersci.com/TFS-Assets/CCG/product-images/NC9331805.JPG-250.jpg)
LGC GENOMICS LLC (Lucigen) TE Buffer, 70 mLStandard buffer for storing nucleic acids (10 mM Tris-HCl [pH 7.5], 1 mM EDTA). To be used with the MasterPure Kits and any other nucleic acid extraction/purification kits.
This reagent is designed for use with the MasterPure™ kit protocols only.
Sigma Aldrich Fine Chemicals Biosciences BES 99.0 titration100G
BES (N N-bis(2-hydroxyethyl)-2-aminoethanesulfonic acid) is a useful secondary standard biochemical buffer. Useful pH range for BES is 6.4 to 7.8. It is useful for diagnostic assay manufacturing industry. BES a sulfonic acid-containing cross-linking agent induces cross-linking between sulfonated polyimide chains in sulfonated polyimide membranes.
Sigma Aldrich Fine Chemicals Biosciences Tris(hydroxymethyl)aminomethane ACS reagent, >=99.8% | 77-86-1 | MFCD00004679 | 100G
Tris(hydroxymethyl)aminomethane ACS reagent, >=99.8% | Purity: >=99.8%; 99.8-100.1% | Mol Wt: 121.14 | 77-86-1 | MFCD00004679 | 100G
Gold Biotechnology Inc Tris HCl 500 g
Tris hydrochloride is a reagent that is used for the stabilization of buffer formulations and electrophoresis of biological molecules
Vmrd Inc 4X FA Powdered Rinse Buffer
FA Rinse Buffer, pH 9.0. Powder to make one liter of 4x rinse buffer. Used to rinse fluorescent antibody antibody substrate slides.
TARGETMOL CHEMICALS INC NVP-TAE 684 10MG
Also available in 2 mg 5 mg 25 mg 50 mg 100 mg 1 mL 10 mM (in DMSO) and bulk. Please contact Fisher for quotes. NVP-TAE 684 (TAE684) is a excellently effective and specific ALK inhibitor(IC50=3 nM). purity: 99%
S2 Media 1L 1x Phosphate Buffered Saline (PBS), pH 6.0, 1L Media Bottle, 1ea
S2Media™ 1x Phosphate Buffered Saline (PBS), pH 6.0 has many applications due to its isotonic nature, including uses as a rinse fluid, diluent, and buffer. Phosphate salts are nontoxic to living cells and have a high buffering capacity. pH maintenance is important in retaining cell viability and in the recovery and revitalization of injured or damaged cells during bacterial culture and analysis. PBS is generally used as a 10mM working solution containing a mixture of sodium chloride and inorganic phosphate salts.• Product meets AOAC, APHA, BAM, CLSI, SM-Dairy, SMWW, and USP-NF reference methods and performance criteria.• For in vitro diagnostic (IVD) use.• PBS should be used by trained professionals within a laboratory setting. • Product is not intended for use in the diagnosis or treatment of disease or other human conditions.
New England Biolabs, Inc. Monarch Buffer WX - 40 ml
The Monarch Buffer WX, a component of Monarch Nucleic Acid Purification Kits, is designed for wash steps in nucleic acid purification. It is supplied as a 5X concentrate and requires dilution with ethanol (>=95%) before use. The product is supplied in 2 bottles, each with 20 ml of concentrated buffer.
- A component of Monarch Nucleic Acid Purification Kits
- Designed for wash steps in nucleic acid purification
- Monarch kit components are also available separately for added convenience
Sigma Aldrich Fine Chemicals Biosciences Tris-Borate-EDTA buffer Bi1L
TBE (Tris/Borate/EDTA) Buffer is commonly used in nucleic acid electrophoresis. This SOLUTIONon is effective under slightly basic conditions which keeps DNA deprotonated water-soluble and protected from degradation. This concentrate can be easily diluted to 1x or 0.5x before use (with molecular biology grade water).
Sigma Aldrich Fine Chemicals Biosciences Dulbeccos Phosphate Bufer500ML
DULBECCOS Dulbeccos Phosphate Bufer500ML
Quality Biological Inc TAE Buffer 50X, 10 Liters
TAE buffer provides faster electrophoretic migration of linear DNA and better resolution of supercoiled DNA. ¿ TAE Buffer may be more suitable than TBE, for electrophoresis if DNA is to be recovered from the agarose and purified using commercially available DNA purification kits. ¿ Applications: TAE buffer has a relatively low buffering capacity, therefore, periodic replacement of the buffer during prolonged electrophoresis is recommended. ¿ Storage Conditions: 15¿C to 30¿C ¿ Shipping Condition: ambient temperature ¿ Sterile Filtered ¿ DNase RNase and Protease Tested ¿ Concentration: 2 M Tris base and 0.05 M disodium EDTA dihydrate ¿ Product and/or packaging contains recycled/renewable content ¿ Product and/or packaging is recyclable or reduces waste ¿ Product and/or packaging is safer to human and environmental health ¿ ¿ Max Shelf Life: 36 months
TRIBIOSCIENCE INC Inoue Transformation Buffer, 500ML
Inoue Transformation Buffer is used for the preparation of high-efficient competent cells. This product is based on the Inoue method for the preparation and transformation of E. Coli component cells.