Life Science Buffers
Balanced salt solutions and powders used for molecular, protein, nucleic acid, and cell biology applications including cell culture, electrophoresis, ELISA, and chromatography processes; available in a variety of formulations and grades.
Buffer solutions serve to keep pH (acidity or alkalinity) nearly constant in a variety of chemical and biological applications. For example, a bicarbonate buffer helps maintain the pH of blood, a buffered saline maintains cellular contents at a consistent pH level, and buffers help maintain a narrow pH range for enzymes to function correctly.
Most biological samples used in research are kept at a pH of about 7.4. Common biological buffers used for tissue culture include Dulbeccos phosphate buffered saline (PBS), Tris base, HEPES, MOPS, PIPES, and other formulations developed for specific cell lines or applications. Most buffers used in cell culture are also DNase-, RNase-, and protease-free.
The choice of buffer for a particular biological reaction or site depends on several factors:
- Temperature
- Desired or target pH
- Buffer toxicity (to the system)
- Buffer interactions with other system components
![Research Products International Corp HEPPS [N-(2-Hydroxyethyl)piperazine-N'-(3-propane sulfonic acid)] [EPPS], 25 Grams](https://assets.fishersci.com/TFS-Assets/CCG/product-images/VN00006095-H75060-25.0.JPG-250.jpg)
Research Products International Corp HEPPS [N-(2-Hydroxyethyl)piperazine-N'-(3-propane sulfonic acid)] [EPPS], 25 Grams
- Substance Name: HEPPS [N-(2-Hydroxyethyl)piperazine-N'-(3-propane sulfonic acid)] [EPPS]
- CAS Number: 16052-06-5
- Molecular Formula: C9H20N2O4S
- Molecular Weight: 252.3
Chem-Impex International, Inc. Ethylenediaminetetraacetic acid | 60-00-4 | MFCD00003541 | 100G
Ethylenediaminetetraacetic acid, 60-00-4, MFCD00003541, 100G
![Research Products International Corp TAE 10X Solution [TRIS Acetate EDTA 10X Solution], 4 Liters](https://assets.fishersci.com/TFS-Assets/CCG/product-images/VN00006095-T60010-4000.011.JPG-250.jpg)
Research Products International Corp TAE 10X Solution [TRIS Acetate EDTA 10X Solution], 4 Liters
TAE 10X Solution is a concentrated Tris-acetate-EDTA buffer used in molecular biology for DNA and RNA electrophoresis. When diluted to 1X, it maintains pH (~8.3) and low ionic strength. It supports clear nucleic acid separation, extended run times, and protects samples from degradation by chelating divalent metal ions like Mg²⁺.
Substance Name: [TRIS Acetate EDTA 10X Solution]
10X solution contains:
TRIS-Base: 0.4 M
Acetic Acid: 0.2 M
EDTA: 10 mM
pH (10X Solution (@25°C): 8.2-8.4
![Research Products International Corp BIS-TRIS Propane [1,3-Bis (TRIS(hydroxymethyl)methylamino)propane], 500 Grams](https://assets.fishersci.com/TFS-Assets/CCG/product-images/VN00006095-B78000-500.0.JPG-250.jpg)
Research Products International Corp BIS-TRIS Propane [1,3-Bis (TRIS(hydroxymethyl)methylamino)propane], 500 Grams
- Substance Name: 1,3-Bis (TRIS(hydroxymethyl)methylamino)propane
- CAS Number: 64431-96-5
- Molecular Formula: C11H26N2O6
- Molecular Weight: 282.3
![Research Products International Corp bis-TRIS [bis-(2-hydroxyethyl)aminotris(hydroxymethyl)methane], 1 Kilogram](https://assets.fishersci.com/TFS-Assets/CCG/product-images/VN00006095-B75000-1000.0.JPG-250.jpg)
Research Products International Corp bis-TRIS [bis-(2-hydroxyethyl)aminotris(hydroxymethyl)methane], 1 Kilogram
Substance Name: bis-(2-hydroxyethyl)aminotris(hydroxymethyl)methane
CAS Number: 6976-37-0
Molecular Formula: C8H19NO5
Molecular Weight:?209
Chemical Purity:?>99%
Soluble in: Water
Store at:?Room Temperature
Sigma Aldrich Fine Chemicals Biosciences Tris Acetate-EDTA buffer 11L
TRIS Tris Acetate-EDTA buffer 11L
Sigma Aldrich Fine Chemicals Biosciences HEPES BioXtra suitable for500G
HEPES has been described as one of the best all-purpose buffers available for biological research. At biological pH the molecule is zwitterionic and is effective as a buffer at pH 6.8 to 8.2 (pKa 7.55). HEPES has been used in a wide variety of applications including tissue culture. It is commonly used to buffer cell culture media in air. HEPES finds its usage in in vitro experiments on Mg. It is typically used in cell culture at concentration between 5mM to 30mM.
Sigma Aldrich Fine Chemicals Biosciences Dulbeccos Modified Eagles1L
This DMEM-Hi glucose medium is a 1x complete medium with no added factors (common modifications) such as L-alanyl-L-glutamine HEPES or sodium pyruvate. It differs from the original DMEM-Hi formulation wherein pyridoxine is substituted for pyridoxal. Pyridoxal is an unstable component of media. This medium requires supplementation with L-glutamine or L-alanyl-L-glutamine.
Teknova 1M Tris-HCl, pH 8.3, 1000mL, Sterile.
Tris (tris(hydroxymethyl)aminomethane) is used as a component ofbuffer solutions, especially for solutions ofnucleic acids. The useful buffer range for tris (pH 7–9) is similar to the pH of most living organisms. This, and its low cost, make tris one of the most common buffers in the biology/biochemistry laboratory.
Quality Biological Inc PBS pH 7.4 without Calcium and Magnesium, 1000 mL
PBS (phosphate buffered saline) ¿ Balanced salt solution used for a variety biological and cell culture applications, such as washing cells before dissociation, transporting cells or tissue, diluting cells for counting, and preparing reagents. ¿ Endotoxin and Mycoplasma tested ¿ Applications: immunological applications, such as Western Blotting. ¿ Storage Condition: 15¿C to 30¿C ¿ Shipping Condition: ambient temperature ¿ Sterile Filtered ¿ Endotoxin and Mycoplasma Tested ¿ pH: 7.3 ¿ 7.5 ¿ Osmolality: 300¿5% mOsm/Kg H2O ¿ Product and/or packaging contains recycled/renewable content ¿ Product and/or packaging is recyclable or reduces waste ¿ Product and/or packaging is safer to human and environmental health ¿ UNSPSC: 12161700 ¿ ¿ Max Shelf Life: 24 months
Abcam Phosphate Buffered Saline (PBS) Casein ELISA Reagent, 100ML
Phosphate Buffered Saline (PBS) Casein ELISA Reagent is a buffer designed for ELISA assay development.
The product is subject to the following: Abcam Restricted Use Statement
Quality Biological Inc PBS (10X), pH 7.4, CS 10 X 500 mL
Balanced salt solution ¿ Wash cells before dissociation ¿ Transport cells or tissue ¿ Dilute cells for counting ¿ Prepare reagents ¿ Endotoxin and Mycoplasma tested ¿ Useful in immunological applications, such as Western Blotting. ¿ Storage Condition: 15¿C to 30¿C ¿ Shipping Condition: ambient temperature ¿ Sterile Filtered ¿ Endotoxin and Mycoplasma Tested ¿ Osmolality: 300 ¿ 55 mOsm/Kg H20 ¿ Product and/or packaging contains recycled/renewable content ¿ Product and/or packaging is recyclable or reduces waste ¿ Product and/or packaging is safer to human and environmental health ¿ UNSPSC: 12161700 ¿ Max Shelf Life: 24 months
Teknova 1X PBS with 4mM EDTA, pH 7.4. 1000mL, Sterile.
Phosphate-buffered saline (PBS) is a salt solution that is commonly used in physiological and biochemical experiments. It can be used to maintain pH neutrality or to buffer against changes in pH in order to provide optimal conditions for cellular growth and function. The buffering range is between pH 6.8 and 7.4.