Life Science Buffers
Balanced salt solutions and powders used for molecular, protein, nucleic acid, and cell biology applications including cell culture, electrophoresis, ELISA, and chromatography processes; available in a variety of formulations and grades.
Buffer solutions serve to keep pH (acidity or alkalinity) nearly constant in a variety of chemical and biological applications. For example, a bicarbonate buffer helps maintain the pH of blood, a buffered saline maintains cellular contents at a consistent pH level, and buffers help maintain a narrow pH range for enzymes to function correctly.
Most biological samples used in research are kept at a pH of about 7.4. Common biological buffers used for tissue culture include Dulbeccos phosphate buffered saline (PBS), Tris base, HEPES, MOPS, PIPES, and other formulations developed for specific cell lines or applications. Most buffers used in cell culture are also DNase-, RNase-, and protease-free.
The choice of buffer for a particular biological reaction or site depends on several factors:
- Temperature
- Desired or target pH
- Buffer toxicity (to the system)
- Buffer interactions with other system components
Biotang Inc E. Coli Lysis Buffer (Tris-HCl 50 mM,1% Triton,EDTA 1 mM, Beta-mercaptoethanol 10 mM), 250 ml
E. Coli Lysis Buffer (Tris-HCl 50 mM,1% Triton,EDTA 1 mM, Beta-mercaptoethanol 10 mM), 250 ml.
Medchemexpress LLC MOPS (sodium salt) | 71119-22-7 | 99.8% | 50 G
MOPS sodium salt is a buffering agent used in biological applications. It helps maintain the pH of mammalian cell culture media and significantly improves the thermal stability of BSA. This product is also suitable for the preparation of keratinocyte and microbial culture media.
- Maintains pH of mammalian cell culture media
- Enhances thermal stability of BSA
- Suitable for preparing keratinocyte culture medium
- Suitable for preparing microbial culture medium
- Can be used in co-culture of keratinocytes and microorganisms
Medchemexpress LLC MOPS | 1132-61-2 | 100.0% | 1 ML
MOPS is a buffering agent utilized in biological applications. It effectively maintains the pH of mammalian cell culture media and significantly enhances the thermal stability of BSA (Bovine Serum Albumin).
- Buffering agent for biological applications.
- Maintains pH of mammalian cell culture media.
- Enhances thermal stability of BSA.
Medchemexpress LLC Mes (2-morpholinoethanesulphonic acid) | 4432-31-9 | 99.85% | 1 ML
MES (2-Morpholinoethanesulphonic acid) is an amphoteric ion buffer designed for various biochemistry and molecular biology applications. It functions effectively within a pH range of 5.5-7.0 and is widely recognized as a Good's buffer, suitable for experiments including cell culture, enzyme activity determination, electrophoresis, and protein studies.
- Amphoteric ion buffer
- Buffer capacity from pH 5.5-7.0
- Ideal for cell culture, enzyme activity determination, electrophoresis, and protein studies
- Solid, white to off-white appearance
- Soluble in water and DMSO
Medchemexpress LLC 2-Morpholinoethanesulphonic acid hydrate | 1266615-59-1 | 99.9% | 50 G
MES hydrate is an amphoteric ion buffer with a buffering capacity ranging from pH 5.5-7.0. It is widely used in biochemistry and molecular biology experiments for applications such as cell culture, enzyme activity determination, electrophoresis, and protein studies.
- Provides effective buffering capacity at approximately pH 6.15.
- Its pKa value changes with temperature, necessitating consideration for different experimental conditions.
- Exhibits good buffering properties in biological experiments.
- Shows poor affinity with metal ions, allowing its use in studies involving them.
Zymo Research Corporation DNA Elution Buffer, 4 ml
This can be used with any of the DNA purification for DNA elution from columns, plates, and magbeads.
Medchemexpress LLC Glycine, N-(2-amino-2-oxoethyl)-N-(carboxymethyl)-, sodium salt (1:2) | 41689-31-0 | 98.0% | 50 G
ADA disodium is a buffer salt derived from adenosine deaminase, commonly used in biochemical research for preparing enzyme buffers. This product is intended for research use only and is not for human or animal therapeutic use.
- Solid appearance
- White to off-white color
- Molecular weight: 234.12
- Chemical formula: C6H8N2Na2O5
- Solubility in water: 350 mg/mL
- Store at 4°C, sealed, away from moisture
- Store in solvent at -80°C for 6 months or -20°C for 1 month, sealed, away from moisture
Biotang Inc Lysis Buffer(His-tag protein purification from E.Coli and insect cells), 500 ml
Lysis Buffer(His-tag protein purification from E.Coli and insect cells), 500 ml.
Sigma Aldrich Fine Chemicals Biosciences ACES >=99.0% (titration) | 7365-82-4 | MFCD00008030 | 25G
ACES >=99.0% (titration) | Purity: >=99.0% (titration) | Mol Wt: 182.2 | 7365-82-4 | MFCD00008030 | 25G
Biotang Inc MES [2-(N-Morpholino)-ethanesulfonic acid], 250 gm
MES [2-(N-Morpholino)-ethanesulfonic acid], 250 gm.
Gold Biotechnology Inc HEPES Free Acid 100 g
HEPES is a buffering agent used in biochemistry and molecular biology that was selected and described by Good et al It is a zwitterionic piperazinic buffer that is useful for a pH range of 6 8 8 2 HEPES lacks the ability to form a significant complex with most metal ions and is recommended for use as a non-coordinating buffer in solutions with metal ions