Life Science Buffers
Balanced salt solutions and powders used for molecular, protein, nucleic acid, and cell biology applications including cell culture, electrophoresis, ELISA, and chromatography processes; available in a variety of formulations and grades.
Buffer solutions serve to keep pH (acidity or alkalinity) nearly constant in a variety of chemical and biological applications. For example, a bicarbonate buffer helps maintain the pH of blood, a buffered saline maintains cellular contents at a consistent pH level, and buffers help maintain a narrow pH range for enzymes to function correctly.
Most biological samples used in research are kept at a pH of about 7.4. Common biological buffers used for tissue culture include Dulbeccos phosphate buffered saline (PBS), Tris base, HEPES, MOPS, PIPES, and other formulations developed for specific cell lines or applications. Most buffers used in cell culture are also DNase-, RNase-, and protease-free.
The choice of buffer for a particular biological reaction or site depends on several factors:
- Temperature
- Desired or target pH
- Buffer toxicity (to the system)
- Buffer interactions with other system components
eMolecules 158144-85-5 | TERT-BUTYL 4-(HYDROXYMETHYL)-4-PHENYLPIPERIDINE-1-CARBOXYLATE | AstaTech | MFCD11044987 | 291.391 | C17H25NO3 | 95.000 | CC(C)(C)OC(=O)N1CCC(CO)(CC1)c1ccccc1 | 1g | 206684235
TERT-BUTYL 4-(HYDROXYMETHYL)-4-PHENYLPIPERIDINE-1-CARBOXYLATE | AstaTech | 158144-85-5 | MFCD11044987 | 291.391 | C17H25NO3 | 95.000 | CC(C)(C)OC(=O)N1CCC(CO)(CC1)c1ccccc1 | 1g | 206684235
![Alkali Scientific TBE 10X Solution [TRIS borate EDTA 10X Solution], 4 Liter Cube Container](https://assets.fishersci.com/TFS-Assets/CCG/product-images/5000661290.jpg-250.jpg)
Alkali Scientific TBE 10X Solution [TRIS borate EDTA 10X Solution], 4 Liter Cube Container
TBE 10X Solution is a highly effective electrophoresis buffer solution, formulated with Tris, borate, and EDTA. This 4-liter cube container provides a concentrated 10X solution that is commonly used for gel electrophoresis of nucleic acids. TBE helps maintain a consistent pH and ionic strength, critical for the accurate migration of DNA and RNA during electrophoresis. The 10X concentration allows for easy dilution to a working concentration of 1X, making it an ideal buffer for high-throughput applications in molecular biology. This large container is perfect for laboratories that perform a high volume of gel electrophoresis and other nucleic acid analysis procedures.
Alkali Scientific CellPro™ Tris Buffer, 2M, pH 7.5, 4L Cubitainer
Alkali Scientific™ CellPro™ Tris solution offers sterile and filtered Tris buffer for various laboratory applications. This high-quality solution is meticulously prepared to ensure purity and consistency, meeting the stringent requirements of scientific research and experimentation. With its sterile formulation, it minimizes the risk of contamination, making it suitable for a wide range of cell culture and molecular biology procedures. The filtered nature of the solution ensures clarity and reliability in experimental results, providing researchers with confidence in their findings. Whether used in protein biochemistry, DNA electrophoresis, or other molecular biology techniques, CellPro™ Tris solution delivers reliable performance and consistent results, contributing to the success of laboratory workflows.
![eMolecules 1365570-27-9 | tert-butyl 5-(hydroxymethyl)-octahydrocyclopenta[c]pyrrole-2-carboxylate | Pharmablock | MFCD22415193 | 241.331 | C13H23NO3 | 97.000 | CC(C)(C)OC(=O)N1CC2CC(CO)CC2C1 | 1g | 551087381](https://assets.fishersci.com/TFS-Assets/CCG/product-images/502157040.JPG-250.jpg)
eMolecules 1365570-27-9 | tert-butyl 5-(hydroxymethyl)-octahydrocyclopenta[c]pyrrole-2-carboxylate | Pharmablock | MFCD22415193 | 241.331 | C13H23NO3 | 97.000 | CC(C)(C)OC(=O)N1CC2CC(CO)CC2C1 | 1g | 551087381
tert-butyl 5-(hydroxymethyl)-octahydrocyclopenta[c]pyrrole-2-carboxylate | Pharmablock | 1365570-27-9 | MFCD22415193 | 241.331 | C13H23NO3 | 97.000 | CC(C)(C)OC(=O)N1CC2CC(CO)CC2C1 | 1g | 551087381
Teknova 50X TAE, Electrophoresis Grade. (2M Tris-Acetate, 100mM EDTA) 1000mL, Non-Sterile. 2 pack.
2M Tris-Acetate, 100mM EDTA
Boston Bioproducts Inc Molecular Biology Grade Water - 2x1L
Molecular Biology Grade Water - 2x1L (For Research Use and Further Manufacturing Only)
Boston Bioproducts Inc Phosphate Buffered Saline, PBS (pH 7.4) - 2x1L
Bioprocess Grade - Phosphate Buffered Saline PBS (pH 7 4) - 2x1L (For Research Use and Further Manufacturing Only)
Surmodics Inc Tris Buffered Saline/Casein Block & Diluent -10X, 1L
BioFX™ Tris Buffered Saline (TBS)/Casein Block and Diluent – 10X Concentrate minimizes non-specific interactions of the secondary reagents with each other and the primary solid phase binding sites. The high blocking efficiency of casein is due to its content of small molecular weight proteins. The solution is ready for dilution to specific application requirements.
Apexbio Technology LLC PBS Liposomes(Synonyms: Phosphate Buffered Saline Liposomes, Empty Liposomes, Blank Liposomes), 5mL.
PBS Liposomes consist of phosphate-buffered saline (PBS) encapsulated within lipid bilayers serving primarily as control reagents in macrophage depletion studies involving clodronate liposomes Unlike clodronate-loaded liposomes PBS liposomes lack active agents targeting macrophages and thus do not induce macrophage apoptosis They function to discriminate between the biological effects mediated by liposomal delivery systems versus those specifically attributable to encapsulated clodronate PBS Liposomes are commonly employed in comparative experimental designs to assess specific macrophage depletion outcomes
Alkali Scientific CellPro™ Tris Buffered Saline 10X, pH 7.4, 1L
Alkali Scientific™ CellPro™ Tris solution offers sterile and filtered Tris buffer for various laboratory applications. This high-quality solution is meticulously prepared to ensure purity and consistency, meeting the stringent requirements of scientific research and experimentation. With its sterile formulation, it minimizes the risk of contamination, making it suitable for a wide range of cell culture and molecular biology procedures. The filtered nature of the solution ensures clarity and reliability in experimental results, providing researchers with confidence in their findings. Whether used in protein biochemistry, DNA electrophoresis, or other molecular biology techniques, CellPro™ Tris solution delivers reliable performance and consistent results, contributing to the success of laboratory workflows.
Bioworld TBE Buffer (25X), 4 L
Product DescriptionTBE (Tris-Borate-EDTA) has a liquid concentrate of 25X, where 1X contains 0.089M Tris, 0.089M Borate, and 0.002M EDTA. 1L of concentrate makes 25L of 1X buffer. Tris solutions are effective buffers for slightly basic conditions. Borate, a derivative of boric acid, is a weak proton donor but a Lewis acid that can accept an electron pair. EDTA is a chelator of divalent cations, mainly Mg2+. TBE is most commonly used for nucleic acid electrophoresis.For laboratory use only. Not fit for agricultural, household, clinical use or human consumption.
Biotang Inc TAE (50X PH 8.0) 1000ML. BM-250. 2 M Tris-base, 1M acetate and 50 mM EDTA, pH 8.0 +/- 0.2. Prepared in 18.2 megohms water and filtered through 1 micron filter.
TAE (50X PH 8.0) 1000ML. BM-250. 2 M Tris-base, 1M acetate and 50 mM EDTA, pH 8.0 +/- 0.2. Prepared in 18.2 megohms water and filtered through 1 micron filter.