Life Science Buffers

Balanced salt solutions and powders used for molecular, protein, nucleic acid, and cell biology applications including cell culture, electrophoresis, ELISA, and chromatography processes; available in a variety of formulations and grades.

Buffer solutions serve to keep pH (acidity or alkalinity) nearly constant in a variety of chemical and biological applications. For example, a bicarbonate buffer helps maintain the pH of blood, a buffered saline maintains cellular contents at a consistent pH level, and buffers help maintain a narrow pH range for enzymes to function correctly.

Most biological samples used in research are kept at a pH of about 7.4. Common biological buffers used for tissue culture include Dulbeccos phosphate buffered saline (PBS), Tris base, HEPES, MOPS, PIPES, and other formulations developed for specific cell lines or applications. Most buffers used in cell culture are also DNase-, RNase-, and protease-free.

The choice of buffer for a particular biological reaction or site depends on several factors:

Temperature
Desired or target pH
Buffer toxicity (to the system)
Buffer interactions with other system components

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256 – 270 of 3,503 results

256

Thermo Scientific Chemicals TRIS-glycine native sample buffer (4X), pH 8.6

For preparation of protein samples for native gel electrophoresis

Pricing & Availability
Specifications

Color	Blue
Physical Form	Liquid
pH	8.6
Chemical Name or Material	Sample Loading Buffer
Concentration	4X
For Use With (Application)	Preparation of protein samples for native gel electrophoresis

257

Thermo Scientific Chemicals Tris(hydroxymethyl)aminomethane, Molecular Biology Grade, 99.9%

CAS: 77-86-1 Molecular Formula: C4H11NO3 Molecular Weight (g/mol): 121.136 MDL Number: MFCD00004679 InChI Key: LENZDBCJOHFCAS-UHFFFAOYSA-N Synonym: TRIS PubChem CID: 6503 ChEBI: CHEBI:9754 IUPAC Name: 2-amino-2-(hydroxymethyl)propane-1,3-diol SMILES: C(C(CO)(CO)N)O

Pricing & Availability
Specifications

PubChem CID	6503
CAS	77-86-1
Molecular Weight (g/mol)	121.136
ChEBI	CHEBI:9754
MDL Number	MFCD00004679
SMILES	C(C(CO)(CO)N)O
Synonym	TRIS
IUPAC Name	2-amino-2-(hydroxymethyl)propane-1,3-diol
InChI Key	LENZDBCJOHFCAS-UHFFFAOYSA-N
Molecular Formula	C4H11NO3

258

Thermo Scientific Chemicals MES, 0.2M buffer soln., pH 6.0

CAS: 145224-94-8 Molecular Formula: C6H15NO5S Molecular Weight (g/mol): 213.25 MDL Number: MFCD00149409 InChI Key: MIIIXQJBDGSIKL-UHFFFAOYSA-N PubChem CID: 16218417 IUPAC Name: 2-(morpholin-4-yl)ethane-1-sulfonic acid hydrate SMILES: O.OS(=O)(=O)CCN1CCOCC1

Pricing & Availability
Specifications

PubChem CID	16218417
CAS	145224-94-8
Molecular Weight (g/mol)	213.25
MDL Number	MFCD00149409
SMILES	O.OS(=O)(=O)CCN1CCOCC1
IUPAC Name	2-(morpholin-4-yl)ethane-1-sulfonic acid hydrate
InChI Key	MIIIXQJBDGSIKL-UHFFFAOYSA-N
Molecular Formula	C6H15NO5S

259

Thermo Scientific Chemicals TRIS, 0.5M buffer soln., pH 6.8

CAS: 77-86-1 Molecular Formula: C4H11NO3 Molecular Weight (g/mol): 121.136 MDL Number: MFCD00004679 InChI Key: LENZDBCJOHFCAS-UHFFFAOYSA-N PubChem CID: 6503 ChEBI: CHEBI:9754 IUPAC Name: 2-amino-2-(hydroxymethyl)propane-1,3-diol SMILES: C(C(CO)(CO)N)O

Pricing & Availability
Specifications

PubChem CID	6503
CAS	77-86-1
Molecular Weight (g/mol)	121.136
ChEBI	CHEBI:9754
MDL Number	MFCD00004679
SMILES	C(C(CO)(CO)N)O
IUPAC Name	2-amino-2-(hydroxymethyl)propane-1,3-diol
InChI Key	LENZDBCJOHFCAS-UHFFFAOYSA-N
Molecular Formula	C4H11NO3

260

TRIS, MP Biomedicals™

TRIS, Molecular Biology Reagent, ≥99.95%, White Crystalline Powder

Pricing & Availability
Specifications

Storage Note 1	Store at Room Temperature (15-30°C). Store dessicated.
Molecular Weight (g/mol)	121.14
Loss on Drying	≤1%
Color	White
Grade	Molecular Biology Reagent
InChI Key	LENZDBCJOHFCAS-UHFFFAOYSA-N
CAS	77-86-1
MDL Number	MFCD00004679
Solubility Information	Soluble in water (40% aq soln) Clear, Colorless Solution (550mg/mL), ethyleneglycol (79.1mg/mL), methanol (26mg/mL), anhydrous ethanol (14.6mg/mL), 95% ethanol (22.0mg/mL), DMF (14mg/mL), acetone (2mg/mL), ethyl acetate (0.5mg/mL), olive oil (0.4mg/mL), and chloroform (0.05mg/mL).
pH	10 to 11.5 (5% aq. soln.)
Synonym	Trometamol,THAM,Tromethamine,Tris base
Purity Grade Notes	≥99.95%
RTECS Number	TY2900000
Beilstein	741883
Molecular Formula	C4H11NO3
Melting Point	167°C to 172°C

261

Gibco™ PBS, pH 7.4

Gibco™ Balanced Salt Solutions are sterile-filtered, packaged in bioprocess containers, and ready to order.

Pricing & Availability
Specifications

Color	Clear, colorless solution
Physical Form	Liquid
pH	7.3 to 7.5
Manufacturing Quality	cGMP
Chemical Name or Material	Phosphate Buffered Saline (PBS)
Osmolality	280 to 315 mOsm/kg
Sterility	Sterile-filtered

262

MilliporeSigma™ Guanidine Hydrochloride, ≥99%, ULTROL™ Grade, Calbiochem™,

Molecular Formula: CH6ClN3 Molecular Weight (g/mol): 95.53 InChI Key: PJJJBBJSCAKJQF-UHFFFAOYSA-N Synonym: Guanidinium Chloride PubChem CID: 5742 ChEBI: CHEBI:32735 IUPAC Name: guanidine;hydrochloride SMILES: C(=N)(N)N.Cl

Pricing & Availability
Specifications

PubChem CID	5742
Molecular Weight (g/mol)	95.53
ChEBI	CHEBI:32735
SMILES	C(=N)(N)N.Cl
Synonym	Guanidinium Chloride
IUPAC Name	guanidine;hydrochloride
InChI Key	PJJJBBJSCAKJQF-UHFFFAOYSA-N
Molecular Formula	CH6ClN3

263

MOPS, 101%, MP Biomedicals™

CAS: 71119-22-7 Molecular Formula: C7H14NNaO4S Molecular Weight (g/mol): 231.242 InChI Key: MWEMXEWFLIDTSJ-UHFFFAOYSA-M Synonym: 3-[N-Morpholino]propanesulfonic acid PubChem CID: 3859613 IUPAC Name: sodium;3-morpholin-4-ylpropane-1-sulfonate SMILES: C1COCCN1CCCS(=O)(=O)[O-].[Na+]

Pricing & Availability
Specifications

PubChem CID	3859613
CAS	71119-22-7
Molecular Weight (g/mol)	231.242
SMILES	C1COCCN1CCCS(=O)(=O)[O-].[Na+]
Synonym	3-[N-Morpholino]propanesulfonic acid
IUPAC Name	sodium;3-morpholin-4-ylpropane-1-sulfonate
InChI Key	MWEMXEWFLIDTSJ-UHFFFAOYSA-M
Molecular Formula	C7H14NNaO4S

264

Thermo Scientific Chemicals TBE urea sample buffer (2X)

Pricing & Availability
Specifications

Color	Blue
Physical Form	Liquid
Chemical Name or Material	Sample Loading Buffer
Concentration	2X

265

MOPSO, 99.5%, MP Biomedicals™

CAS: 79803-73-9 Molecular Formula: C7H14NNaO5S Molecular Weight (g/mol): 247.241 InChI Key: WSFQLUVWDKCYSW-UHFFFAOYSA-M Synonym: sodium 2-hydroxy-3-morpholinopropane-1-sulfonate,mopso sodium salt,3-morpholino-2-hydroxypropanesulfonic acid sodium salt,3-n-morpholinyl-2-hydroxypropanesulfonic acid sodium salt,sodium 2-hydroxy-3-morpholin-4-yl propane-1-sulfonate,mopso sodium salt titration,mopso, 0.2m buffer solution, ph 6.0,mopso, 0.2m buffer solution, ph 6.5,mopso, 0.2m buffer solution, ph 7.0,mopso, 0.2m buffer solution, ph 7.5 IUPAC Name: sodium;2-hydroxy-3-morpholin-4-ylpropane-1-sulfonate

Pricing & Availability
Specifications

CAS	79803-73-9
Molecular Weight (g/mol)	247.241
Synonym	sodium 2-hydroxy-3-morpholinopropane-1-sulfonate,mopso sodium salt,3-morpholino-2-hydroxypropanesulfonic acid sodium salt,3-n-morpholinyl-2-hydroxypropanesulfonic acid sodium salt,sodium 2-hydroxy-3-morpholin-4-yl propane-1-sulfonate,mopso sodium salt titration,mopso, 0.2m buffer solution, ph 6.0,mopso, 0.2m buffer solution, ph 6.5,mopso, 0.2m buffer solution, ph 7.0,mopso, 0.2m buffer solution, ph 7.5
IUPAC Name	sodium;2-hydroxy-3-morpholin-4-ylpropane-1-sulfonate
InChI Key	WSFQLUVWDKCYSW-UHFFFAOYSA-M
Molecular Formula	C7H14NNaO5S

266

Tricine, 0.5M buffer soln., pH 6.5, Thermo Scientific™

CAS: 4-1-5704 Molecular Formula: C6H13NO5 Molecular Weight (g/mol): 179.17 MDL Number: MFCD00004277 InChI Key: SEQKRHFRPICQDD-UHFFFAOYSA-N PubChem CID: 79784 ChEBI: CHEBI:39063 IUPAC Name: 2-[[1,3-dihydroxy-2-(hydroxymethyl)propan-2-yl]amino]acetic acid SMILES: C(C(=O)O)NC(CO)(CO)CO

Pricing & Availability
Specifications

PubChem CID	79784
CAS	4-1-5704
Molecular Weight (g/mol)	179.17
ChEBI	CHEBI:39063
MDL Number	MFCD00004277
SMILES	C(C(=O)O)NC(CO)(CO)CO
IUPAC Name	2-[[1,3-dihydroxy-2-(hydroxymethyl)propan-2-yl]amino]acetic acid
InChI Key	SEQKRHFRPICQDD-UHFFFAOYSA-N
Molecular Formula	C6H13NO5

267

BES, 0.5M buffer soln., pH 6.0, Thermo Scientific™

CAS: 66992-27-6 Molecular Formula: C6H14NNaO5S Molecular Weight (g/mol): 235.23 MDL Number: MFCD00067425 InChI Key: CFQLQLSIZOWFNV-UHFFFAOYSA-M PubChem CID: 23671640 IUPAC Name: sodium;2-[bis(2-hydroxyethyl)amino]ethanesulfonate SMILES: C(CO)N(CCO)CCS(=O)(=O)[O-].[Na+]

Pricing & Availability
Specifications

PubChem CID	23671640
CAS	66992-27-6
Molecular Weight (g/mol)	235.23
MDL Number	MFCD00067425
SMILES	C(CO)N(CCO)CCS(=O)(=O)[O-].[Na+]
IUPAC Name	sodium;2-[bis(2-hydroxyethyl)amino]ethanesulfonate
InChI Key	CFQLQLSIZOWFNV-UHFFFAOYSA-M
Molecular Formula	C6H14NNaO5S

268

ADA, 0.2M buffer soln., pH 6.5, Thermo Scientific™

CAS: 7415-22-7 Molecular Formula: C6H9N2NaO5 Molecular Weight (g/mol): 212.137 MDL Number: MFCD00065483 InChI Key: QCGKKVWCABJQQI-UHFFFAOYSA-M PubChem CID: 23679048 IUPAC Name: sodium;2-[(2-amino-2-oxoethyl)-(carboxymethyl)amino]acetate SMILES: C(C(=O)N)N(CC(=O)O)CC(=O)[O-].[Na+]

Pricing & Availability
Specifications

PubChem CID	23679048
CAS	7415-22-7
Molecular Weight (g/mol)	212.137
MDL Number	MFCD00065483
SMILES	C(C(=O)N)N(CC(=O)O)CC(=O)[O-].[Na+]
IUPAC Name	sodium;2-[(2-amino-2-oxoethyl)-(carboxymethyl)amino]acetate
InChI Key	QCGKKVWCABJQQI-UHFFFAOYSA-M
Molecular Formula	C6H9N2NaO5