Life Science Buffers
Balanced salt solutions and powders used for molecular, protein, nucleic acid, and cell biology applications including cell culture, electrophoresis, ELISA, and chromatography processes; available in a variety of formulations and grades.
Buffer solutions serve to keep pH (acidity or alkalinity) nearly constant in a variety of chemical and biological applications. For example, a bicarbonate buffer helps maintain the pH of blood, a buffered saline maintains cellular contents at a consistent pH level, and buffers help maintain a narrow pH range for enzymes to function correctly.
Most biological samples used in research are kept at a pH of about 7.4. Common biological buffers used for tissue culture include Dulbeccos phosphate buffered saline (PBS), Tris base, HEPES, MOPS, PIPES, and other formulations developed for specific cell lines or applications. Most buffers used in cell culture are also DNase-, RNase-, and protease-free.
The choice of buffer for a particular biological reaction or site depends on several factors:
- Temperature
- Desired or target pH
- Buffer toxicity (to the system)
- Buffer interactions with other system components
Biotang Inc Tricine-buffered Saline (5X) pH 8.0, 250 ml
Tricine-buffered Saline (5X) pH 8.0, 250 ml.
Medchemexpress LLC Nvp-TAE 684 | 761439-42-3 | MFCD11977634 | 99.7% | 614.20 | C30H40ClN7O3S | 1 ML
NVP-TAE 684 is a potent, selective small-molecule inhibitor of anaplastic lymphoma kinase (ALK) used in preclinical research to block the growth of ALCL-derived and ALK-dependent cell lines (reported IC50 2-10 nM). It is provided as both solid material and as a ready-to-use 10 mM solution in DMSO, and is characterized for purity suitable for in vitro studies.
- Potent ALK inhibition with low nanomolar IC50 values.
- Selective activity against ALK-dependent cell lines.
- Available as a 10 mM solution in DMSO for ready-to-use experiments.
- High purity (99.7%) suitable for research applications.
- Molecular weight 614.20 Da and formula C30H40ClN7O3S.
- Offered in multiple solid pack sizes for flexible dosing and storage.
- Documentation available including data sheet and SDS.
Bioworld TTETRIS-TAPS-EDTA) BUFFER
This item has a minimum order quantity of 2 per supplier requirements. TTE (TRIS-TAPS-EDTA) BUFFER POWDER BLEND: - Proteomics Grade - An electrophoresis running buffer for DNA Sequencing that yields longer reads
Crescent Chemical Co Inc TRIS BUFFER PH 8.0 1 M SOLN
Tris buffer pH 8.0, 1 M solution molecular biology grade DNase, RNase, Protease not detected. The pH value of Tris buffer is temperature and concentration dependent. For Tris buffers, pH increases about 0.03 unit per degree C decrease in temperature, and decreases 0.03 - 0.05 uni
![Alkali Scientific TBE 10X Solution [TRIS borate EDTA 10X Solution], 10 L](https://assets.fishersci.com/TFS-Assets/CCG/product-images/5000660679.jpg-250.jpg)
Alkali Scientific TBE 10X Solution [TRIS borate EDTA 10X Solution], 10 L
TBE 10X Solution (TRIS Borate EDTA) is a powerful buffering solution designed for use in electrophoresis, particularly for separating nucleic acids such as DNA and RNA. This 10-liter bottle provides a concentrated formula of Tris, borate, and EDTA, which maintain pH and ionic strength during electrophoretic separation. The TBE buffer ensures consistent and effective separation of nucleic acid samples by maintaining a stable environment for the molecules to migrate through the gel matrix. This large 10-liter container is ideal for laboratories requiring bulk quantities for high-throughput electrophoresis or other molecular biology techniques.
Biotang Inc TRIS-Buffered Saline (TBS, 10X) For Western Blot Washing; (NaCl 1.5M Tris 0.5M), 1000 ml
TRIS-Buffered Saline (TBS, 10X) For Western Blot Washing; (NaCl 1.5M Tris 0.5M), 1000 ml.