Life Science Buffers
Balanced salt solutions and powders used for molecular, protein, nucleic acid, and cell biology applications including cell culture, electrophoresis, ELISA, and chromatography processes; available in a variety of formulations and grades.
Buffer solutions serve to keep pH (acidity or alkalinity) nearly constant in a variety of chemical and biological applications. For example, a bicarbonate buffer helps maintain the pH of blood, a buffered saline maintains cellular contents at a consistent pH level, and buffers help maintain a narrow pH range for enzymes to function correctly.
Most biological samples used in research are kept at a pH of about 7.4. Common biological buffers used for tissue culture include Dulbeccos phosphate buffered saline (PBS), Tris base, HEPES, MOPS, PIPES, and other formulations developed for specific cell lines or applications. Most buffers used in cell culture are also DNase-, RNase-, and protease-free.
The choice of buffer for a particular biological reaction or site depends on several factors:
- Temperature
- Desired or target pH
- Buffer toxicity (to the system)
- Buffer interactions with other system components
ILEX LIFE SCIENCES LLC TRYPSIN 10X P10-022100 100ML
PAN-Biotech (10x) Trypsin 2.5 % in PBS, w/o: Ca and Mg (6 x 100 ml). DESCRIPTION: PAN-Biotech (10x) Trypsin 2.5% in PBS, w/o: Ca and Mg is a porcine pancreas-derived enzyme in a 10x concentrated PBS solution. It is commonly used for the dissociation of anchorage-dependent (adherent) mammalian cells and tissues from culture surfaces. The concentration of trypsin necessary to dislodge cells from their substrate is dependent on the cell type and the age of the culture. APPLICATION: Enzymatic solution for the dissociation and disaggregation of anchorage-dependent mammalian cells and tissues. Trypsin solutions can range from 0.025% to 0.5% depending on application. LIQUID/POWDER: Liquid. PHENOL RED: Without Phenol Red. Ca/Mg CONTENT: Without Ca and Mg. TRYPSIN: 2.50% Trypsin. EDTA: No EDTA. STERILE: Yes. STORAGE TEMP: -20°C. MANUFACTURER: PAN-Biotech GmbH (Germany).
National Diagnostics 1 liter size, TAE Buffer (50X) 0.2 micron filtration
Buffer; National Diagnostics; TAE (50X); Formulated w/18 Megohm water and 0.2u filtration; Saves time and improves results; Certified RNase and DNase free; Size: 1L
Chrono Log Corporation Saline (0.9%), 15mL
This item has a minimum qty of 4 per supplier requirements.
Sterile physiological saline for irrigation (0.85% or 0.9% w/v) for CHRONOPAR Reagent preparation and for dilution of the Whole Blood specimen.
MilliporeSigma END COVER PINCH PIPES,3/8X 5/8
NovaSeal™ End cover pinch pipes, 3/8 in. ID x 5/8 in. OD
Sigma Aldrich Fine Chemicals Biosciences CHES 99.0 (titration), 103-47-9, MFCD00003835
CHES 99.0 (titration)
Sigma Aldrich Fine Chemicals Biosciences Tris Buffered Saline, with Tween 20, pH 8.0 powder
Tris Buffered Saline, with Tween 20, pH 8.0 powder
Sigma Aldrich Fine Chemicals Biosciences Tris-EDTA buffer solution 100X concentrate for Northern and Southern blotting, MFCD00236359
Tris-EDTA buffer solution 100X concentrate for Northern and Southern blotting
Boston Bioproducts Inc Tris-Acetate Buffer (0.2 M, pH 7.0) - 500ml
The Tris-Acetate Buffer is a buffering agent composed of Tris-base and acetic acid. The combination of these ingredients yields a pH range of 6.0-8.0. This buffer is ideal for requiring a pH slightly above physiological conditions. Often used to support the electrophoresis of nucleic acids on agarose gel and protein electrophoresis employing SDS-PAGE and related blotting techniques.
United States Biological Corporation TRIS HCL BUFFER 1M PH7.5,500ML
Tris is one of the most widely used buffers in molecular biology and cell culture due to its low toxicity, stability and buffering capacity. Purity: ≥99.9% Appearance: White, crystalline powder pH (1M): 10.5-11.5 pKa: 8.0-8.4 Absorbance (260nm): ≤0.06 Absorbance (280nm): ≤0.05 Absorbance (290nm): ≤0.2 Solubility (1M): Colorless, clear, complete Loss on Drying: ≤0.2% Melting Point: 168-172°C Heavy Metals (Pb): ≤0.0001% Arsenic: ≤0.0001% Calcium: ≤0.0001% Copper: ≤0.0001% Iron: ≤0.0005% Magnesium: ≤0.0001% Insoluble Matter: ≤0.005% Residue on Ignition: ≤0.1% RNase: DNase: Protease: Meets specifications.
Quality Biological Inc PBS pH 7.2, 4 Liters
PBS (phosphate buffered saline) is a balanced salt solution ¿ Used for a variety biological and cell culture applications, such as washing cells before dissociation, transporting cells or tissue, diluting cells for counting, and preparing reagents ¿ Endotoxin and Mycoplasma tested ¿ Also useful in immunological applications, such as Western Blotting ¿ Storage Condition: 15¿C ¿ 30¿C ¿ Shipping Condition: ambient temperature ¿ Sterile Filtered ¿ Endotoxin and Mycoplasma Tested ¿ pH: 7.1 -7.3 ¿ Osmolality: 300¿5% mOsm/Kg H2O ¿ Product and/or packaging contains recycled/renewable content Product and/or packaging is recyclable or reduces waste ¿ Product and/or packaging is safer to human and environmental health ¿ UNSPSC: 12161700 ¿ ¿ Max Shelf Life: 24 months
Boston Bioproducts Inc Tris Buffered Saline, TBS (10X, pH 7.4, Low Endotoxin) - 250ml
The Tris Buffered Saline (TBS) is an isotonic non-toxic buffer solution used commonly in biochemical immunoassay as a wash buffer. Tris maintains a stable pH range typically between 7.4 to 8.0 while NaCl regulates osmotic balance via its elected concentration. This TBS Solution is formulated at a 10X concentration and tested for endotoxin.
Sigma Aldrich Fine Chemicals Biosciences HEPES, 7365-45-9, MFCD00006158, 100g
Empirical Formula (Hill Notation) C8H18N2O4S, ≥99.5% (titration), Molecular Weight 238.30, Synonym: 4-(2-Hydroxyethyl)piperazine-1-ethanesulfonic acid, N-(2-Hydroxyethyl)piperazine-N′-(2-ethanesulfonic acid)