Life Science Buffers
Balanced salt solutions and powders used for molecular, protein, nucleic acid, and cell biology applications including cell culture, electrophoresis, ELISA, and chromatography processes; available in a variety of formulations and grades.
Buffer solutions serve to keep pH (acidity or alkalinity) nearly constant in a variety of chemical and biological applications. For example, a bicarbonate buffer helps maintain the pH of blood, a buffered saline maintains cellular contents at a consistent pH level, and buffers help maintain a narrow pH range for enzymes to function correctly.
Most biological samples used in research are kept at a pH of about 7.4. Common biological buffers used for tissue culture include Dulbeccos phosphate buffered saline (PBS), Tris base, HEPES, MOPS, PIPES, and other formulations developed for specific cell lines or applications. Most buffers used in cell culture are also DNase-, RNase-, and protease-free.
The choice of buffer for a particular biological reaction or site depends on several factors:
- Temperature
- Desired or target pH
- Buffer toxicity (to the system)
- Buffer interactions with other system components
Research Products International Corp Tricine-SDS Sample Buffer 2X Solution, 25 ML
2X Formulation:
SDS: 8%
Glycerol: 20%
Coomassie Blue: 0.01%
Phenol Red: 0.01%
Sigma Aldrich Fine Chemicals Biosciences Dulbeccos Modified Eagles500ML
This modification has been shown to improve the stability of the medium.
Apexbio Technology LLC Annexin V-APC/PI Apoptosis Kit 20tests
Annexin V is a Ca2 -dependent phospholipid-binding protein that specifically binds to phosphatidylserine (PS) Normally PS is localized on the inner leaflet of the cell membrane lipid bilayer During the early stages of apoptosis PS translocates from the inner leaflet to the outer surface of the cell membrane exposing it to the extracellular environment Due to Annexin V s high affinity for PS fluorescently labeled Annexin V (APC) can be used as a probe in combination with flow cytometry or fluorescence microscopy for detecting apoptosis and the staining process requires only 10-20 minutes Additionally this kit can be paired with the nuclear dye propidium iodide (PI) PI specifically binds to double-stranded DNA emitting red fluorescence upon binding but it cannot normally permeate intact cell membranes However PI can penetrate compromised cellular membranes in late-stage apoptotic and dead cells staining nuclei Therefore combined staining with Annexin V-APC and PI allows
Sigma Aldrich Fine Chemicals Biosciences Dulbeccos Phosphate Bufer500ML
Dulbeccos Phosphate Bufer500ML
Boston Bioproducts Inc Phosphate Buffered Saline, PBS (Sterile-Filtered, Low Endotoxin) - 250ml
Phosphate Buffered Saline PBS (Sterile-Filtered Low Endotoxin) - 250ml (For Research Use and Further Manufacturing Only)
Apexbio Technology LLC Annexin V-APC Reagent 20tests
Annexin V is a Ca2 -dependent phospholipid-binding protein that specifically binds phosphatidylserine (PS) Under normal conditions PS is located on the inner side of the plasma membrane lipid bilayer however during early apoptosis PS translocates from the inner leaflet to the outer surface of the cell membrane becoming exposed to the extracellular environment Annexin V has a high affinity for PS Fluorescent (APC)-labeled Annexin V serves as a fluorescent probe enabling detection of apoptotic cells using flow cytometry or fluorescence microscopy The staining procedure takes only 15-30 minutes to complete Use of this reagent requires additional purchase of 10X Binding Buffer (Catalog no K2284) To further characterize different apoptosis stages Annexin V can be combined with nuclear dyes such as 7-AAD DAPI PI or SYTOX Green These nuclear dyes specifically bind double-stranded DNA generating fluorescence Normally they cannot penetrate intac
Sigma Aldrich Fine Chemicals Biosciences Dulbeccos Modified Eagles1L
This modification has been shown to improve the stability of the medium.
ILEX LIFE SCIENCES LLC EDTA P10-15100 100ML
PAN-Biotech EDTA 1:5000 (1X), 0.2 g/L in PBS, w/o Ca and Mg (100 ml). DESCRIPTION: PAN-Biotech EDTA 1:5000 (1X), 0.2 g/L in PBS, w/o Ca and Mg. Ethylenediaminetetraacetic acid (EDTA), a chelating agent, is often used to remove calcium from cell washing and suspension media. EDTA can reduce clumping of cells grown in liquid suspension. It is also used for gentle detachment of certain adherent cells for passaging. EDTA is often added to trypsin solutions to enhance enzymatic activity by neutralizing calcium and magnesium ions that enhance cell-to-cell adhesion and obscure the peptide bonds on which trypsin acts. LIQUID/POWDER: Liquid. PHENOL RED: Without Phenol Red. Ca/Mg CONTENT: Without Ca and Mg. EDTA: 0.02% EDTA. STERILE: Yes. STORAGE TEMP: +2-8°C. MANUFACTURER: PAN-Biotech GmbH (Germany).
Sigma Aldrich Fine Chemicals Biosciences Phosphate buffered saline
PHOSPHATE Phosphate buffered saline
Hach Company pPb-1 Acid Preservative Solution, 236 mL
For Lead determination by the LeadTrak™ Fast Column Extraction method. Hach method 8317.
GenDEPOT Sodium phosphate dibasic | 7558-79-4 | 141.959 g/mol | Not specified
Sodium phosphate dibasic | 7558-79-4 | 141.959 g/mol | Not specified