Life Science Buffers
Balanced salt solutions and powders used for molecular, protein, nucleic acid, and cell biology applications including cell culture, electrophoresis, ELISA, and chromatography processes; available in a variety of formulations and grades.
Buffer solutions serve to keep pH (acidity or alkalinity) nearly constant in a variety of chemical and biological applications. For example, a bicarbonate buffer helps maintain the pH of blood, a buffered saline maintains cellular contents at a consistent pH level, and buffers help maintain a narrow pH range for enzymes to function correctly.
Most biological samples used in research are kept at a pH of about 7.4. Common biological buffers used for tissue culture include Dulbeccos phosphate buffered saline (PBS), Tris base, HEPES, MOPS, PIPES, and other formulations developed for specific cell lines or applications. Most buffers used in cell culture are also DNase-, RNase-, and protease-free.
The choice of buffer for a particular biological reaction or site depends on several factors:
- Temperature
- Desired or target pH
- Buffer toxicity (to the system)
- Buffer interactions with other system components
Crescent Chemical Co Inc MORPHOLINOETHANE SULFONIC ACID
Morpholinoethane sulfonic acid analytical grade pKa 20 = 6.15. Buffering substance for biochemistry and molecular biology (1). Physical constants (2). Size - 1KG Storage Conditions - +15 °C TO +30 °C Catalog Number - 29834.03 CAS 4432-31-9
Medchemexpress LLC HEPES, NaCl, Sucrose, pH 7.2 buffer | 100 ML
This physiological buffer is composed of 20 mM HEPES, 150 mM NaCl, and 240 mM Sucrose, adjusted to pH 7.2. It is primarily used to resuspend human erythrocyte ghost preparations.
- Physiological buffer
- Composed of HEPES, NaCl, and Sucrose
- Adjusted to pH 7.2
- Used to resuspend human erythrocyte ghost preparations
Thermo Scientific Production Chemicals and Services 50 MM TRIS-HCL 10 MM EDTA 5ML
NC3588252 50 MM TRIS-HCL 10 MM EDTA 5ML
Crescent Chemical Co Inc TRIS(HYDROXYMETHYL)AMINOMETHAN
Tris(hydroxymethyl)aminomethane.hydrochloride molecular biology grade DNase/RNase Protease not detected. Size - 500G Storage Conditions - +15 °C TO +30 °C Catalog Number - 39787.01 CAS 1185-53-1
Bioworld STE Buffer (10X), 4 L
STE ReagentCommon reagent for molecular biology. Used in DNA extraction, DNA electrophoresis and many other lab protocols.DNAse and RNAse free, 10X solutionTechnical Information:1X concentration solution containsNaCl (100mM) Tris-HCl, pH 8.0 (10mM) EDTA, pH 8.0 (1mM)
Bioworld TBS Buffer, Low Salt (10X), 4 L
Product DescriptionTris-buffered saline (TBS) is low salt used for Western blot washing with a concentration of 10X. It contains 500 mM Tris, 1.5M NaCl and has a pH level of 7.4.TBS is isotonic and non-toxic, and is used to dilute substances. This buffer is used as a washing buffer for electrodes and to wash microtiter plates for Western blot experiments and screening methods.
LAB BIOREAGENTS NGB II Buffer 100ml
Lab Bioreagents NGB (I, II, III) are newly developed next generation assay buffers that provides exceptional performance and broad compatibility for Western blot, ELISA, IHC and nucleic acid detection assays. The NGBs are basic buffers that hat requires neutralization by acid in the reaction. Among the tests, NGBs have been demonstrated to maintain the full bioactivity of proteins and antibodies.