Life Science Buffers
Balanced salt solutions and powders used for molecular, protein, nucleic acid, and cell biology applications including cell culture, electrophoresis, ELISA, and chromatography processes; available in a variety of formulations and grades.
Buffer solutions serve to keep pH (acidity or alkalinity) nearly constant in a variety of chemical and biological applications. For example, a bicarbonate buffer helps maintain the pH of blood, a buffered saline maintains cellular contents at a consistent pH level, and buffers help maintain a narrow pH range for enzymes to function correctly.
Most biological samples used in research are kept at a pH of about 7.4. Common biological buffers used for tissue culture include Dulbeccos phosphate buffered saline (PBS), Tris base, HEPES, MOPS, PIPES, and other formulations developed for specific cell lines or applications. Most buffers used in cell culture are also DNase-, RNase-, and protease-free.
The choice of buffer for a particular biological reaction or site depends on several factors:
- Temperature
- Desired or target pH
- Buffer toxicity (to the system)
- Buffer interactions with other system components
ILEX LIFE SCIENCES LLC PAN Biotech DPBS without Ca and Mg GMP Grade 500 ML 1/EA
PAN-Biotech DPBS without Ca and Mg GMP Grade 500 ml DESCRIPTION Dulbecco s Phosphate-Buffered Saline (DPBS) without calcium chloride and magnesium chloride GMP Grade Ready-to-use phosphate-buffered saline solution designed for direct use in GMP-regulated clinical and industrial applications It ensures reliable maintenance of pH and osmolality and provides essential inorganic ions required for safe and reproducible cell handling Combined with a very low endotoxin level high batch-to-batch consistency and comprehensive documentation DPBS GMP represents a reliable ready-to-use solution that can be immediately implemented in sensitive manufacturing and clinical workflows without additional preparation steps QUALITY GMP Grade (EU Annex 1) BICARBONATE Without Sodium Bicarbonate CALCIUM/MAGNESIUM Without Ca and Mg LIQUID/POWDER Liquid PHENOL RED Without Phenol Red STERILE Yes STORAGE 2 C - RT MANUFACTURER PAN-Biotech GmbH COUNTRY OF ORIGIN Germany
Alkali Scientific CellPro™ PBS 10X, pH 7.0, 500mL, 500mL, 10/case
CellPro™ PBS 10X, pH 7.0, 500mL Phosphate-buffered saline (PBS) is a balanced salt solution that is used for a variety of cell culture applications, such as washing cells before dissociation, transporting cells or tissue samples, diluting cells for counting, and preparing reagents. PBS is formulated without calcium and magnesium for rinsing chelators from the culture before cell dissociation. Concentrated forms of Gibco™ PBS may require pH adjustment at the time of dilution.
Boston Bioproducts Inc Sodium Phosphate Buffer (0.2 M, pH 7.0) - 1L
The Sodium Phosphate Buffer is a versatile reagent with a high buffering capacity, commonly used across various scientific disciplines across biochemistry and molecular biology. This buffer has a pH range of 5.8 to 8.0. It's applications include protein analysis, where it supports solubility, stability, and activity. It is also compatible with biologically relevant metal ions that are significant for cellular or physiological conditions.
Apexbio Technology LLC Protease Inhibitor Cocktail (EDTA-Free 100X in DMSO) 100x1ml
Cell crude extracts contain various endogenous enzymes such as phosphatases and proteases capable of degrading proteins present in the extracts The optimal strategy to enhance intact protein yield is adding specific inhibitors targeting these enzymes This Protease Inhibitor Cocktail is used in fungal and yeast extracts to improve protein stability by inhibiting proteases responsible for degrading both phosphorylated and non-phosphorylated protein substrates This Protease Inhibitor Cocktail consists of individual compounds including AEBSF 1 10-Phenanthroline E-64 and Pepstatin A which provide broad-spectrum specific inhibition of cysteine proteases serine proteases aspartic proteases and metalloproteases It is optimized and tested for fungal and yeast extracts This cocktail is available as a ready-to-use solution dissolved in DMSO
Seracare Life Sciences Inc 10X DULBECCO S PBS (D-PBS)
10X Phosphate-buffered Saline (PBS) is ideal for use in sample preparation and as a wash buffer in general immunoassay applications as well as tissue and cell culture procedures. This modified Dulbecco's buffer is not formulated with magnesium or calcium salts. pH=7.4. Conductivity controlled.
Alkali Scientific CellPro™ DPBS Buffer, 1X, with Calcium and Magnesium, 500mL, 10/Case
Phosphate Buffered Saline is a buffer solution commonly used in biological research. PBS has many uses because it is isotonic and non-toxic to most cells. These uses include substance dilution and cell container rinsing.
Scytek Laboratories ScyTek Phosphate Buffered Saline (25x), 7.6 pH, 1000ml
ScyTek Phosphate Buffered Saline (25x) pH of 7.6 is an optimal formulation of pH stabilizers and salts designed to effectively remove excess material from the microtiter plate wells without disrupting the ELISA binding reaction. By maintaining the proper buffering environment, unbound components can be washed away without suppressing antigen-antibody binding interactions, thereby reducing nonspecific background and increasing the specific signal. Our Wash buffers do not contain hazardous preservatives such as Azide or Mercury that may interfere with antibody-antigen binding interactions. For your convenience wash buffer is offered in a wide variety of formulations to meet the needs of your specific ELISA application. This product can be customized to meet the specific needs of your assay. Inquire about custom vialing, labeling, kit assembly and drop shipping.
![Alkali Scientific PBS 1X Solution [Phosphate Buffered Saline 1X Sterile Solution], 500 Milliliters](https://assets.fishersci.com/TFS-Assets/CCG/product-images/5000660133.jpg-250.jpg)
Alkali Scientific PBS 1X Solution [Phosphate Buffered Saline 1X Sterile Solution], 500 Milliliters
PBS (Phosphate Buffered Saline) 1X Solution is a sterile, isotonic buffer solution used in a variety of biological applications. The 500 mL volume is convenient for smaller-scale applications, including cell culture, molecular biology, and immunology experiments. PBS helps maintain the osmotic balance and pH of biological systems, preventing cell lysis or shrinkage. It is frequently used to wash cells, dilute samples, and prepare reagents for biochemical assays. PBS is an essential component in maintaining physiological conditions in in vitro studies and ensuring that cells, tissues, and biomolecules remain stable during handling and experimental procedures.
Biotang Inc Carbonate Buffered Saline 5X, pH 9.5. 15 mM Sodium Carbonate, 750 mM Sodium Chloride, pH 9.5. Prepared in 18.2 megohms water and filtered through 0.22 micron filter. BB-535
Carbonate Buffered Saline 5X, pH 9.5. 15 mM Sodium Carbonate, 750 mM Sodium Chloride, pH 9.5. Prepared in 18.2 megohms water and filtered through 0.22 micron filter. BB-535.
Teknova 1M HEPES, pH 7.5, 20L Bag, Sterile. 4 Pack.
HEPES solution is a Goods buffer that is commonly used in cell culture and biochemical applications. It is well known for its ability to maintain pH level, making it an essential part of any experiment that requires a stable pH. It has a buffering range of 6.8 - 8.2.
Alkali Scientific MOPSO Sodium Salt, 500 G
MOPSO Sodium Salt, 500 G, is a high-quality buffering reagent used to stabilize pH in laboratory experiments, particularly those involving proteins, nucleic acids, and enzyme reactions. MOPSO is known for its ability to maintain a stable pH in the range of 6.5 to 7.9, which is essential for many biochemical assays and protocols. This larger 500 g package is ideal for high-volume or repeated experiments and provides a cost-effective solution for laboratories working on large-scale studies or industrial applications. MOPSO is widely used in cell culture, protein analysis, and other critical research areas requiring reliable pH control.
Bioworld ACES, 100 g
ACES, High Purity Grade N-[carbamoylmethyl]-2-Aminoethane-sulfonic acidpKa: (20°C): 6.8+0.1Appearance: White crystalsHS Code : 29241900Melting Point: >220 °C (dec.)(lit.)PH Value: 6.1-7.5