Life Science Buffers
Balanced salt solutions and powders used for molecular, protein, nucleic acid, and cell biology applications including cell culture, electrophoresis, ELISA, and chromatography processes; available in a variety of formulations and grades.
Buffer solutions serve to keep pH (acidity or alkalinity) nearly constant in a variety of chemical and biological applications. For example, a bicarbonate buffer helps maintain the pH of blood, a buffered saline maintains cellular contents at a consistent pH level, and buffers help maintain a narrow pH range for enzymes to function correctly.
Most biological samples used in research are kept at a pH of about 7.4. Common biological buffers used for tissue culture include Dulbeccos phosphate buffered saline (PBS), Tris base, HEPES, MOPS, PIPES, and other formulations developed for specific cell lines or applications. Most buffers used in cell culture are also DNase-, RNase-, and protease-free.
The choice of buffer for a particular biological reaction or site depends on several factors:
- Temperature
- Desired or target pH
- Buffer toxicity (to the system)
- Buffer interactions with other system components
Sigma Aldrich Fine Chemicals Biosciences PIPES | 5625-37-6 | MFCD00006159 | 100g
PIPES | 99% | MW: 307.32 | 5625-37-6 | MFCD00006159 | 100g
Sigma Aldrich Fine Chemicals Biosciences PIPES BioXtra, for molecular biology, >=99.5% (T) | 5625-37-6 | MFCD00006159 | 50G
PIPES BioXtra, for molecular biology, >=99.5% (T) | Purity: >=99.5% (T) | Mol Wt: 302.37 | 5625-37-6 | MFCD00006159 | 50G
Quality Biological Inc DPBS (10X) without Calcium and Magnesium, 10 Liters
DPBS is a balanced salt solution used for the handling and culturing of mammalian cells. DPBS without calcium and magnesium can be used to wash and rinse suspended cells. irrigating, transporting or diluting fluid for mammalian cells, while maintaining cell tonicity and viability for a limited period of time.
Without Calcium and Magnesium
Without Phenol Red
Storage Condition: 15 °C to 30 °C
Shipping Condition: ambient temperature
Sterile Filtered
Endotoxin and Mycoplasma Tested
Max Shelf Life: 24 months from date of manufacture
Manufactured under an ISO 9001:2015 certified quality management system.
Product and/or packaging contains recycled/renewable content. Product and/or packaging is recyclable or reduces waste. Product and/or packaging is safer to human and environmental health.
Sigma Aldrich Fine Chemicals Biosciences Trizma base | 77-86-1 | MFCD00004679 | 100g
Trizma base | Purity: 99.9% | Mol Wt: 121.41 | 77-86-1 | MFCD00004679 | 100g
Sigma Aldrich Fine Chemicals Biosciences HEPES solution BioPerformance Certified, 1 M, suitable for cell culture, 0.2 mum filtered | 7365-45-9 | MFCD00006158 | 1L
HEPES solution BioPerformance Certified, 1 M, suitable for cell culture, 0.2 mum filtered | 7365-45-9 | MFCD00006158 | 1L
CARTERRA INC 0.1 M MES PH 5.5
NC2232564 0.1 M MES PH 5.5
Sigma Aldrich Fine Chemicals Biosciences MOPS | 1132-61-2 | MFCD00006183 | 500g
MOPS | Purity: ≥99.5% (titration) | MW:209.26 | 1132-61-2 | MFCD00006183 | 500g
Sigma Aldrich Fine Chemicals Biosciences MOPS anhydrous, free-flowing, Redi-Dri(TM), >=99.5% | 1132-61-2 | MFCD00006183 | 2.5KG
MOPS anhydrous, free-flowing, Redi-Dri(TM), >=99.5% | Purity: >=99.5% | Mol Wt: 209.26 | 1132-61-2 | MFCD00006183 | 2.5KG
Hampton Research HR2-733/1.0 M HEPES sodium pH 7.5 - 100 ml
HR2-733/1.0 M HEPES sodium pH 7.5 - 100 ml. Please note items from this supplier are non-returnable.
Quality Biological Inc TBS HCl (10X), pH 7.2, 500 mL
Tris HCl (10X), pH 7.2 is a Buffered Saline Solution used in procedures for the isolation of DNA.
Filtered through a 0.22 µm membrane
DNase, RNase, and Protease tested
Applications: Tris buffered saline is useful for the following: as a biological diluent buffer, as a buffer for antibodies, as a wash buffer for immunological assays including western blot, immunohistochemistry, immunofluorescence microscopy, and ELISA.
Storage Conditions: 15 °C to 30 °C
Shipping Condition: ambient temperature
Sterile Filtered
DNase, RNase and Protease Tested
Max Shelf Life: 36 months from date of manufacture
Product and/or packaging contains recycled/renewable content Product and/or packaging is recyclable or reduces waste Product and/or packaging is safer to human and environmental health
![Research Products International Corp ACES [N-(2-Acetamido)-2-aminoethane], 100 Grams](https://assets.fishersci.com/TFS-Assets/CCG/product-images/VN00006095-A22500-100.0.JPG-250.jpg)
Research Products International Corp ACES [N-(2-Acetamido)-2-aminoethane], 100 Grams
Zwitterionic buffer used in biochemistry and molecular biology research.
- pKa (25°C): 6.88
- The working pH range in aqueous solution is 6.1 - 7. 5
- Absorbance @ 280nm (5% water): <0.02
Electron Microscopy Sciences R-Universal Epitope Recovery Buffer (10x stock) 500 ML
EMS UNIVERSAL Epitope Recovery Buffer is a novel (patent-pending) technology for epitope recovery, primarily based on reversing the fixation effects of formaldehyde, which created links primarily between ε-amino groups of lysine and other amino-groups. In extensive testing the buffer has shown excellent results when used with different antibodies, including those that require low or high pH buffers or protolithic treatment for successful staining.
The EMS Universal Buffer may be used in any heat-induced epitope recovery system (the time and temperature of treatment should be tested), but was specifically adjusted for tissue sections processing in our Antigen Retriever (62700 Series). Using our Universal Buffer in our Retriever guarantees the highest rate of success in recovering epitope for any antibody, especially one that was never previously used on formalin-fixed sections. Shelf Life: 12 months.