Life Science Buffers
Balanced salt solutions and powders used for molecular, protein, nucleic acid, and cell biology applications including cell culture, electrophoresis, ELISA, and chromatography processes; available in a variety of formulations and grades.
Buffer solutions serve to keep pH (acidity or alkalinity) nearly constant in a variety of chemical and biological applications. For example, a bicarbonate buffer helps maintain the pH of blood, a buffered saline maintains cellular contents at a consistent pH level, and buffers help maintain a narrow pH range for enzymes to function correctly.
Most biological samples used in research are kept at a pH of about 7.4. Common biological buffers used for tissue culture include Dulbeccos phosphate buffered saline (PBS), Tris base, HEPES, MOPS, PIPES, and other formulations developed for specific cell lines or applications. Most buffers used in cell culture are also DNase-, RNase-, and protease-free.
The choice of buffer for a particular biological reaction or site depends on several factors:
- Temperature
- Desired or target pH
- Buffer toxicity (to the system)
- Buffer interactions with other system components
Alkali Scientific SSC Buffer, 20X Solution, 1 L
SSC Buffer 20X Solution (1 L) is a ready-to-use, concentrated buffer solution widely used in DNA hybridization experiments. This 20X concentration allows for easy dilution to the desired working concentration in hybridization assays, including Southern and Northern blotting. The high ionic strength provided by SSC Buffer stabilizes nucleic acid interactions, ensuring that probes bind specifically to complementary sequences during hybridization. The 1-liter volume is suitable for routine laboratory use, offering a convenient quantity for hybridization experiments and nucleic acid detection protocols. It is an essential reagent in molecular biology for accurate and reliable results.
Boston Bioproducts Inc Molecular Biology Grade Water - 2x1L
Molecular Biology Grade Water - 2x1L (For Research Use and Further Manufacturing Only)
ATHENA ES HBS w/Phenol Red, 500 ml
Hepes Buffered Saline is available with or without Phenol Red. It is an isotonic saline buffered with Hepes, for use in rinsing cell monolayers prior to cell dissociation using PETTM or washing harvested cells.
Medchemexpress LLC 1H-1,2,3-Triazole, 1-(3-iodophenyl)-4-(3-nitrophenyl)- | 1809031-84-2 | 99.5% | 392.15 | 10 MG
P62-mediated mitophagy inducer (PMI) is a P62-mediated mitophagy activator that activates mitochondrial autophagy without recruitment of Parkin or collapse of the mitochondrial membrane potential. It remains active in cells lacking a fully functional PINK1/Parkin pathway and serves as a pharmacological tool to study the molecular mechanisms of mitosis, avoiding toxicity and some of the non-specific effects associated with the sudden dissipation of mitochondria lacking membrane potential.
- P62-mediated mitophagy activator
- Activates mitochondrial autophagy without recruitment of Parkin or collapse of the mitochondrial membrane potential
- Remains active in cells lacking a fully functional PINK1/Parkin pathway
- Serves as a pharmacological tool to study the molecular mechanisms of mitosis
- Avoids toxicity and some non-specific effects associated with the sudden dissipation of mitochondria lacking membrane potential
Thermo Scientific Production Chemicals and Services 50MM TRIS 10MM EDTA PH 7.2
NC3588255 50MM TRIS 10MM EDTA PH 7.2
LAB BIOREAGENTS LowBinding I Buffer 500ml
Lab Bioreagents LowBindings (I, II, III, IV, V, VI, VII) are newly developed assay buffers that provides exceptional performance and broad compatibility for Western blot, ELISA, IHC and nucleic acid detection assays. The LowBindings helps to reduce the non-specific bindings, negative effects of interfering molecules, and cross-reactivities of antibodies and proteins from biomatrix. Therefore, LowBindings exhibit the low matrix interference and high signal-to-noise, by which the assay sensitivity and specificity have been significantly improved. As many factors are involved in immunoassays, there is no single assay buffer that is ideal for each assay. LowBindings (I, II, III, IV, V, VI, VII) are a variety of buffers based on different formulations.
TARGETMOL CHEMICALS INC NVP-TAE 226 5MG
Also available in 1 mL, 1 mg, 2 mg, 10 mg, 25 mg, 50 mg, 100 mg and bulk. Please contact Fisher for quotes. NVP-TAE 226 (TAE226) is a potent FAK inhibitor (IC50 5.5 nM), exhibiting even greater efficacy against Pyk2 (IC50 3.5 nM), and is 10- to 100-fold less effective against IGF-1R, InsR, c-Met, and ALK. Purity 98.07%
Crescent Chemical Co Inc EDTA ANALYTICAL GRADE
Ethylenediamine tetraacetic acid analytical grade Size - 100G Storage Conditions - +15 °C TO +30 °C Catalog Number - 11278.01 CAS 60-00-4