Life Science Buffers
Balanced salt solutions and powders used for molecular, protein, nucleic acid, and cell biology applications including cell culture, electrophoresis, ELISA, and chromatography processes; available in a variety of formulations and grades.
Buffer solutions serve to keep pH (acidity or alkalinity) nearly constant in a variety of chemical and biological applications. For example, a bicarbonate buffer helps maintain the pH of blood, a buffered saline maintains cellular contents at a consistent pH level, and buffers help maintain a narrow pH range for enzymes to function correctly.
Most biological samples used in research are kept at a pH of about 7.4. Common biological buffers used for tissue culture include Dulbeccos phosphate buffered saline (PBS), Tris base, HEPES, MOPS, PIPES, and other formulations developed for specific cell lines or applications. Most buffers used in cell culture are also DNase-, RNase-, and protease-free.
The choice of buffer for a particular biological reaction or site depends on several factors:
- Temperature
- Desired or target pH
- Buffer toxicity (to the system)
- Buffer interactions with other system components
ILEX LIFE SCIENCES LLC EDTA P10-15100 6X100ML
PAN-Biotech EDTA 1:5000 (1X), 0.2 g/L in PBS, w/o Ca and Mg (6 x 100 ml). DESCRIPTION: PAN-Biotech EDTA 1:5000 (1X), 0.2 g/L in PBS, w/o Ca and Mg. Ethylenediaminetetraacetic acid (EDTA), a chelating agent, is often used to remove calcium from cell washing and suspension media. EDTA can reduce clumping of cells grown in liquid suspension. It is also used for gentle detachment of certain adherent cells for passaging. EDTA is often added to trypsin solutions to enhance enzymatic activity by neutralizing calcium and magnesium ions that enhance cell-to-cell adhesion and obscure the peptide bonds on which trypsin acts. LIQUID/POWDER: Liquid. PHENOL RED: Without Phenol Red. Ca/Mg CONTENT: Without Ca and Mg. EDTA: 0.02% EDTA. STERILE: Yes. STORAGE TEMP: +2-8°C. MANUFACTURER: PAN-Biotech GmbH (Germany).
Alkali Scientific Neutralizing Buffered Peptone Water (NBPW), Sterile, Pre Enrichment Medium with Disinfectant Neutralizers, Ready to Use (Bottled) Microbiology Diluent, 25mL, 25/Case
Neutralizing Buffered Peptone Water (NBPW), Sterile, Pre Enrichment Medium with Disinfectant Neutralizers, Ready to Use (Bottled) Microbiology Diluent, 25mL, 25/Case. This 25mL bottle contains a special formula designed to neutralize disinfectants while enriching microbial samples. Its sterile condition ensures no contamination, and the ready-to-use format reduces preparation time, making it an ideal solution for microbiological laboratories. Perfect for researchers requiring neutralization of disinfectants.
Boston Bioproducts Inc Saline-Sodium Citrate (20X) - 4L
Saline-Sodium Citrate (20X) - 4L (For Research Use and Further Manufacturing Only)
Alkali Scientific CellPro™ 1X Balanced Salt Solution, 500ml, 10/Case
Balanced salt solution for cell culture applications. All formulations incorporate a rigorous specification and approval process. Available in lot sizes that facilitate scale-up and manufacturing.
Biotang Inc Phosphate-Buffered Saline (PBS, 10X)For Western Blot Washing, 1000 ml
Phosphate-Buffered Saline (PBS, 10X)For Western Blot Washing, 1000 ml.
ILEX LIFE SCIENCES LLC TRYPSIN P10-021500 500ML
PAN-Biotech Trypsin 0.25% in PBS, w/o: Ca and Mg (500 ml). DESCRIPTION: PAN-Biotech Trypsin 0.25% in PBS, w/o: Ca and Mg is a porcine pancreas-derived enzyme in PBS solution. It is commonly used for the dissociation of anchorage-dependent (adherent) mammalian cells and tissues from culture surfaces. The concentration of trypsin necessary to dislodge cells from their substrate is dependent on the cell type and the age of the culture. APPLICATION: Enzymatic solution for the dissociation and disaggregation of anchorage-dependent mammalian cells and tissues. Trypsin solutions can range from 0.025% to 0.5% depending on application. LIQUID/POWDER: Liquid. PHENOL RED: Without Phenol Red. Ca/Mg CONTENT: Without Ca and Mg. TRYPSIN: 0.25% Trypsin. EDTA: No EDTA. STERILE: Yes. STORAGE TEMP: -20°C. MANUFACTURER: PAN-Biotech GmbH (Germany).
Biocare Medical, LLC Immunocare PBS, 10X
Immunocare PBS Wash Buffer (sodium azide free) works well for manual and automated immunohistochemistry applications as well as other laboratory procedures requiring a high quality PBS buffer with superior pH stability. The PBS contains surfactant and allows for reagents to spread more uniformly across tissue section on slides. It can also be used on an automated staining system.
Apexbio Technology LLC HyperScript RT SuperMix for qPCR (with gDNA wiper) 50 rxns
HyperScript RT SuperMix for qPCR is a reverse transcription reaction premix based on HyperScript Reverse Transcriptase a novel enzyme engineered from M-MLV (RNase H-) Reverse Transcriptase featuring significantly reduced RNase H activity and greatly enhanced thermo-stability Due to its improved heat tolerance HyperScript Reverse Transcriptase is especially suitable for reverse transcription of RNA templates with complex secondary structures HyperScript RT SuperMix for qPCR (with gDNA wiper) is specifically designed for two-step qRT-PCR assays The 5 SuperMix contains all necessary components for reverse transcription Simply adding template RNA and RNase-Free ddH2O allows rapid reaction initiation The included 4 gDNA wiper efficiently eliminates potential genomic DNA contamination from samples rapidly and thoroughly prior to reverse transcription The kit is particularly suitable for reverse transcription reactions from RNA templates of low concentration
Apexbio Technology LLC Annexin V-HF488/DAPI Apoptosis Kit 20assays
Annexin V is a Ca2 -dependent phospholipid-binding protein that specifically binds to phosphatidylserine (PS) Normally PS is localized on the inner leaflet of the cell membrane lipid bilayer During the early stages of apoptosis PS translocates from the inner leaflet to the outer surface of the cell membrane exposing it to the extracellular environment Due to Annexin V s high affinity for PS fluorescently labeled Annexin V (HF488) can be used as a fluorescent probe to detect apoptotic cells through flow cytometry or fluorescence microscopy with staining taking only 10-20 minutes DAPI specifically binds to double-stranded DNA emitting blue fluorescence and is generally unable to permeate intact cell membranes However during late apoptosis or cell death DAPI can penetrate compromised membranes enabling nuclear staining Therefore combined staining with Annexin V-HF488 and DAPI facilitates the differentiation of cells in different apoptosis stages as well as dead cells
Medchemexpress LLC NaCl, KCl, Phosphate | AR/GR grade | 10 POUCHES
MCE PBS Powder (2 L of 1x) consists of NaCl, KCl, and Phosphate, and is widely used in immunoassay, microbiology, and protein experiments. The PBS buffer prepared from this product can be directly used for cell culture and other related experiments after being sterilized by 0.22 μm filtration. Each pouch can be diluted to 2 L of 1x PBS buffer, resulting in a concentration of 137 mM NaCl, 2.7 mM KCl, and 10 mM Phosphate. This product offers a convenient method for making PBS buffer, eliminating the need to weigh and mix individual components.
- Consists of NaCl, KCl, and Phosphate.
- Convenient way to make PBS buffer.
- Eliminates the need to weigh and mix individual components.
- All relevant raw materials are AR/GR grade.
- The pH of the 1x PBS buffer is 7.4 ± 0.2 @ 25°C.