Life Science Buffers
Balanced salt solutions and powders used for molecular, protein, nucleic acid, and cell biology applications including cell culture, electrophoresis, ELISA, and chromatography processes; available in a variety of formulations and grades.
Buffer solutions serve to keep pH (acidity or alkalinity) nearly constant in a variety of chemical and biological applications. For example, a bicarbonate buffer helps maintain the pH of blood, a buffered saline maintains cellular contents at a consistent pH level, and buffers help maintain a narrow pH range for enzymes to function correctly.
Most biological samples used in research are kept at a pH of about 7.4. Common biological buffers used for tissue culture include Dulbeccos phosphate buffered saline (PBS), Tris base, HEPES, MOPS, PIPES, and other formulations developed for specific cell lines or applications. Most buffers used in cell culture are also DNase-, RNase-, and protease-free.
The choice of buffer for a particular biological reaction or site depends on several factors:
- Temperature
- Desired or target pH
- Buffer toxicity (to the system)
- Buffer interactions with other system components
Gold Biotechnology Inc MOPS, Sodium Salt 1 kg
MOPS sodium salt is a buffering agent used in biochemistry and molecular biology that was selected and described by Good et al. It is a zwitterionic, morpholinic buffer that is useful for a pH range of 6.5 – 7.9 and commonly used for cell culture media, as a running buffer in electrophoresis and for protein purification in chromatography. MOPS lacks the ability to form a complex with most metal ions and is recommended for use as a non-coordinating buffer in solutions with metal ions. MOPS is often used in buffered culture media for bacteria, yeast, and mammalian cells. MOPS is regarded as an excellent buffer for use in separating RNA in agarose gels. It is recommended to sterilize MOPS buffers by filtration rather than with autoclave due to the unknown identity of yellow degradation products that occur after sterilization of MOPS with autoclave. It is suitable for use in the bicinchoninic acid (BCA) assay.
Medchemexpress LLC AR-13324 analog mesylate | 2095432-73-6 | 98.0% | 631.72 | 50 MG
AR-13324 analog mesylate is an analog of AR-13324. AR-13324 is an ROCK and norepinephrine transporter (NET) inhibitor.
- Analog of AR-13324.
- Ar-13324 is a rock and norepinephrine transporter (net) inhibitor.
- Available in various sizes, including 50 mg.
Chem-Impex International, Inc. N-(2-Acetamido)-2-aminoethanesulfonic acid | 7365-82-4 | MFCD00008030 | 250G
N-(2-Acetamido)-2-aminoethanesulfonic acid, 7365-82-4, MFCD00008030, 250G
Chem-Impex International, Inc. N-(2-Acetamido)-2-aminoethanesulfonic acid | 7365-82-4 | MFCD00008030 | 1KG
N-(2-Acetamido)-2-aminoethanesulfonic acid, 7365-82-4, MFCD00008030, 1KG
Bioworld Tris-Glycine-SDS (TG-SDS) Buffer, Ready-Pack (10X), 1 PK(s)
Tris Glycine-SDS BufferTD-SDS Buffer is blended using bioPLUS reagents to provide the highest quality buffer for protein research. Ideal for protein separations, this buffer is formulated to ensure exceptional purity in the absence of nuclease and protease activities.TG-SDS Buffer is a widely used running buffer for denaturing electrophoresis of proteins (SDS-PAGE).Technical Information:Storage: RTHazard: Irritant1 pack makes 1 L of 10x concentrate.1X Solution Contains: 0.025 M Tris, 0.192 M Glycine and 0.1% SDS.
Bioworld TBE Buffer (10X), 1 L
Product DescriptionTBE (Tris-Borate-EDTA) has a liquid concentrate of 10X, where 1X contains 0.089M Tris, 0.089M Borate, and 0.002M EDTA. 1L of concentrate makes 10L of 1X buffer. Tris solutions are effective buffers for slightly basic conditions. Borate, a derivative of boric acid, is a weak proton donor but a Lewis acid that can accept an electron pair. EDTA is a chelator of divalent cations, mainly Mg2+. TBE is most commonly used for nucleic acid electrophoresis.For laboratory use only. Not fit for agricultural, household, clinical use or human consumption.
Dojindo Molecular Technologies Inc HEPES (100 G), Good's buffer, CAS: 7365-45-9, Purity: ≥99.0% (Titration), pKa=7.55, Active pH 6.8-8.2, made in Japan
Buffers perform an important task in biological experiments, often designed to keep pH within the 6-8 range where most biological reactions occur. Dr. Norman Good, the developer of HEPES, wanted to create buffers that prioritized this function, while also providing other benefits. Dr. Good produced a series of “Good buffers” – including HEPES (2-[4-(2-Hydroxyethyl)-1-piperazinyl]ethanesulfonic acid) – that are nearly ubiquitous in their usage even now. The Good series of buffers share the following additional characteristics: 1) High water-solubility. 2) Low cell membrane permeability. 3) Consistent acid-base dissociation constants. 4) Low metal-chelating capability. 5) High chemical stability. 6)Low absorption spectra in UV and visible regions.
Alkali Scientific Bicine Ultra Pure, 500 G
Bicine Ultra Pure in a 500g size is a high-purity biological buffer, formulated for optimal performance in a wide range of laboratory applications. This buffer is commonly used in protein assays, electrophoresis, and DNA/RNA work due to its excellent buffering capacity within the pH range of 7.6–9.0. The 500g package is ideal for high-throughput research laboratories that require a reliable and cost-effective source of buffer for large-scale operations. Bicine's minimal background interference and low ionic strength make it perfect for sensitive applications. This product ensures consistent pH regulation, vital for experimental reproducibility in molecular biology, biochemistry, and pharmaceutical research.
![Medchemexpress LLC 1-Propanesulfonic acid, 2-hydroxy-3-[[2-hydroxy-1,1-bis(hydroxymethyl)ethyl]amino]- | 68399-81-5 | 98.0% | 25 G](https://assets.fishersci.com/TFS-Assets/CCG/product-images/5000414468-logo.png-250.jpg){kind=logo}
Medchemexpress LLC 1-Propanesulfonic acid, 2-hydroxy-3-[[2-hydroxy-1,1-bis(hydroxymethyl)ethyl]amino]- | 68399-81-5 | 98.0% | 25 G
TAPSO is a common biological buffer and pH stabilization reagent. It has an effective pH ranging from 7-8 and pKa values ranging from 7.5-9.0. This compound contains Tris groups and exhibits reactive activity with zwitterionic glycine peptides.
- Common biological buffer
- pH stabilization reagent
- Effective pH ranging from 7-8
- pKa values ranging from 7.5-9.0
- Contains Tris groups
- Reactive with zwitterionic glycine peptides
- Suitable for research applications
Medchemexpress LLC 2-Morpholinoethanesulphonic acid hemisodium | 117961-21-4 | 98.0% | 50 G
2-Morpholinoethanesulphonic acid hemisodium | 117961-21-4 | 98.0% | 50 G
Medchemexpress LLC Heppso | 68399-78-0 | 99.9% | 5 G
HEPPSO is a zwitterionic buffer with a working pH range of 7.1-8.5. It exhibits a relatively high ability to bind copper(II) and has a pKa of 7.84 at a 2.0 mM buffer concentration.
- It is a zwitterionic buffer.
- Its working pH range is 7.1-8.5.
- It displays relatively high ability to bind copper(II).
- It has a pKa of 7.84 at 2.0 mM buffer concentration.
- Available in solid form, white to off-white in color.
- Store powder at -20°C for 3 years or 4°C for 2 years.
- Store in solvent at -80°C for 2 years or -20°C for 1 year.
![Bioworld ACES (N-[carbamoylmethyl]-2-Aminoethane-sulfonic acid) Buffer, 0.5M, pH 6.2, 500 mL](https://assets.fishersci.com/TFS-Assets/CCG/product-images/50255312.jpg-250.jpg)
Bioworld ACES (N-[carbamoylmethyl]-2-Aminoethane-sulfonic acid) Buffer, 0.5M, pH 6.2, 500 mL
Synonym:N-(2-Acetamido)-2-aminoethanesulfonic acid; N-(Carbamoylmethyl)-2-aminoethanesulfonic acid; N-(Carbamoylmethyl)taurineACES is one ofGood's buffersdeveloped in the 1960s to provide buffers withpHranging from 6.15-8.35 for use in various applications. Azwitterionicbuffer with apKaof 6.9, it is often used as abuffering agentinbiologicalandbiochemicalresearch. ACES had been used to develop buffers for bothagaroseandpolyacrylamide gel electrophoresis.A saturated solution contains 0.22 M at 0°C. ACES binds Cu2+, but not significantly Mg2+, Ca2+or Mn2+.Appearance: LiquidHS Code : 29241900Melting Point: >220 °C (dec.)(lit.)PH Value: 6.0
Bioworld ADA (N-(2-acetamido)iminodiacetic acid) Buffer, 0.2M, pH 7.5, 500 mL
Synonym:ADA-Na,N-(2-Acetamido)iminodiacetic acid monosodium salt,N-(Carbamoylmethyl)iminodiacetic acid monosodium saltA zwitterionic buffer used in biological applications including protein isolation and characterizationWarning: For laboratory use only.Not fit for agricultural, household, clinical use or human consumption. Appearance: LiquidPH Value: 7.5