Life Science Buffers
Balanced salt solutions and powders used for molecular, protein, nucleic acid, and cell biology applications including cell culture, electrophoresis, ELISA, and chromatography processes; available in a variety of formulations and grades.
Buffer solutions serve to keep pH (acidity or alkalinity) nearly constant in a variety of chemical and biological applications. For example, a bicarbonate buffer helps maintain the pH of blood, a buffered saline maintains cellular contents at a consistent pH level, and buffers help maintain a narrow pH range for enzymes to function correctly.
Most biological samples used in research are kept at a pH of about 7.4. Common biological buffers used for tissue culture include Dulbeccos phosphate buffered saline (PBS), Tris base, HEPES, MOPS, PIPES, and other formulations developed for specific cell lines or applications. Most buffers used in cell culture are also DNase-, RNase-, and protease-free.
The choice of buffer for a particular biological reaction or site depends on several factors:
- Temperature
- Desired or target pH
- Buffer toxicity (to the system)
- Buffer interactions with other system components
Bioworld MODIFIED DULBECCO S PBS PACKS
Modified Dulbecco\'s PBS Packs are pouches of dry-blend powder that are each sufficient to make 500mL of physiological Na- and K-phosphate buffered saline (D-PBS) for ELISA and Western blot diluents and wash buffers. Dry-Blend Packs of m...
Biocare Medical, LLC Immunocare PBS, 10X
Immunocare PBS Wash Buffer (sodium azide free) works well for manual and automated immunohistochemistry applications as well as other laboratory procedures requiring a high quality PBS buffer with superior pH stability. The PBS contains surfactant and allows for reagents to spread more uniformly across tissue section on slides. It can also be used on an automated staining system.
Teknova 1X PBS Solution, pH 7.4. 100mL, Sterile. 10 pack.
Phosphate-buffered saline (PBS) is a salt solution that is commonly used in physiological and biochemical experiments. It can be used to maintain pH neutrality or to buffer against changes in pH in order to provide optimal conditions for cellular growth and function. The buffering range is between pH 6.8 and 7.4.
Teknova 1X PBS Solution with 185mM Sodium Chloride, pH 7.4. 1000mL, Sterile 2 pack.
Phosphate-buffered saline (PBS) is a salt solution that is commonly used in physiological and biochemical experiments. It can be used to maintain pH neutrality or to buffer against changes in pH in order to provide optimal conditions for cellular growth and function. The buffering range is between pH 6.8 and 7.4.
Medchemexpress LLC NaCl, KCl, Phosphate | AR/GR grade | 10 POUCHES
MCE PBS Powder (2 L of 1x) consists of NaCl, KCl, and Phosphate, and is widely used in immunoassay, microbiology, and protein experiments. The PBS buffer prepared from this product can be directly used for cell culture and other related experiments after being sterilized by 0.22 μm filtration. Each pouch can be diluted to 2 L of 1x PBS buffer, resulting in a concentration of 137 mM NaCl, 2.7 mM KCl, and 10 mM Phosphate. This product offers a convenient method for making PBS buffer, eliminating the need to weigh and mix individual components.
- Consists of NaCl, KCl, and Phosphate.
- Convenient way to make PBS buffer.
- Eliminates the need to weigh and mix individual components.
- All relevant raw materials are AR/GR grade.
- The pH of the 1x PBS buffer is 7.4 ± 0.2 @ 25°C.
![Alkali Scientific PBS [Phosphate Buffered Saline] 200mL Tablets, 500 Tablets per Bottle](https://assets.fishersci.com/TFS-Assets/CCG/product-images/5000660121.jpg-250.jpg)
Alkali Scientific PBS [Phosphate Buffered Saline] 200mL Tablets, 500 Tablets per Bottle
PBS [Phosphate Buffered Saline] 200mL Tablets are designed for laboratories that need to prepare larger volumes of PBS quickly and accurately. Each tablet dissolves easily in water to create 200 mL of phosphate-buffered saline, a standard buffer used in many biological and biochemical applications. The 500-tablet bottle is perfect for high-throughput labs that require a consistent and reliable buffer solution. These tablets ensure that the PBS solution maintains the correct pH and osmolarity, which is essential for applications such as cell culture, protein assays, and molecular biology experiments. The tablets are convenient, cost-effective, and reduce the chance of errors in solution preparation.
![Alkali Scientific PBS [Phosphate Buffered Saline], 1X Solution, 1.0ml Pre Filled Tubes, Sterile, 36 Tubes per Case, 4.5ml Tube](https://assets.fishersci.com/TFS-Assets/CCG/product-images/5000660239.jpg-250.jpg)
Alkali Scientific PBS [Phosphate Buffered Saline], 1X Solution, 1.0ml Pre Filled Tubes, Sterile, 36 Tubes per Case, 4.5ml Tube
PBS (Phosphate Buffered Saline) 1X Solution is a sterile, ready-to-use solution supplied in pre-filled 1.0mL tubes for laboratory use. The 36-tube pack is designed for applications requiring quick and convenient aliquots of PBS without the risk of contamination. PBS is essential for maintaining the osmotic balance and pH stability of biological samples during handling, washing, and processing. This product is commonly used in cell culture, microbiological assays, and molecular biology techniques. The pre-filled tube format minimizes handling and preparation time, making it ideal for high-throughput research environments, diagnostic labs, and any setting where reproducibility and precision are crucial.