Life Science Buffers
Balanced salt solutions and powders used for molecular, protein, nucleic acid, and cell biology applications including cell culture, electrophoresis, ELISA, and chromatography processes; available in a variety of formulations and grades.
Buffer solutions serve to keep pH (acidity or alkalinity) nearly constant in a variety of chemical and biological applications. For example, a bicarbonate buffer helps maintain the pH of blood, a buffered saline maintains cellular contents at a consistent pH level, and buffers help maintain a narrow pH range for enzymes to function correctly.
Most biological samples used in research are kept at a pH of about 7.4. Common biological buffers used for tissue culture include Dulbeccos phosphate buffered saline (PBS), Tris base, HEPES, MOPS, PIPES, and other formulations developed for specific cell lines or applications. Most buffers used in cell culture are also DNase-, RNase-, and protease-free.
The choice of buffer for a particular biological reaction or site depends on several factors:
- Temperature
- Desired or target pH
- Buffer toxicity (to the system)
- Buffer interactions with other system components
![eMolecules 23141-25-5 | STRICTOSAMIDE | AstaTech | MFCD14105616 | 498.532 | C26H30N2O8 | 95.000 | OC[C@H]1O[C@@H](O[C@@H]2OC=C3[C@@H](C[C@@H]4N(CCc5c4[nH]c4ccccc54)C3=O)[C@H]2C=C)[C@H](O)[C@@H](O)[C@@H]1O | 1mg | 718087441](https://assets.fishersci.com/TFS-Assets/CCG/product-images/502460896.JPG-250.jpg)
eMolecules 23141-25-5 | STRICTOSAMIDE | AstaTech | MFCD14105616 | 498.532 | C26H30N2O8 | 95.000 | OC[C@H]1O[C@@H](O[C@@H]2OC=C3[C@@H](C[C@@H]4N(CCc5c4[nH]c4ccccc54)C3=O)[C@H]2C=C)[C@H](O)[C@@H](O)[C@@H]1O | 1mg | 718087441
STRICTOSAMIDE | AstaTech | 23141-25-5 | MFCD14105616 | 498.532 | C26H30N2O8 | 95.000 | OC[C@H]1O[C@@H](O[C@@H]2OC=C3[C@@H](C[C@@H]4N(CCc5c4[nH]c4ccccc54)C3=O)[C@H]2C=C)[C@H](O)[C@@H](O)[C@@H]1O | 1mg | 718087441
Chem-Impex International, Inc. 3-N-Morpholinopropanesulfonic acid | 1132-61-2 | MFCD00006183 | 100G
3-N-Morpholinopropanesulfonic acid, 1132-61-2, MFCD00006183, 100G
Sigma Aldrich Fine Chemicals Biosciences BICINE Redi DriTM 99 free 1KG
BICINE Redi DriTM 99 free 1KG
Medchemexpress LLC Heppso | 68399-78-0 | 99.89% | 100 G
HEPPSO is a zwitterionic buffer with a working pH range of 7.1-8.5. It exhibits a relatively high ability to bind copper(II) and possesses a pKa of 7.84 at a 2.0 mM buffer concentration. It is intended for research use only and is not sold to patients.
- Working pH range of 7.1-8.5.
- Exhibits a relatively high ability to bind copper(II).
- Possesses a pKa of 7.84 at a 2.0 mM buffer concentration.
- For research use only.
- Molecular weight: 268.33.
- Formula: C9H20N2O5S.
- Appearance: Solid.
- Color: White to off-white.
Medchemexpress LLC HEPPS | 16052-06-5 | 99.0% | 25 G
HEPPS (EPPS) is a buffering agent with a useful pH range from 7.3 to 8.7. It reduces Aβ-aggregate-induced memory deficits and rescues cognitive deficits in mice. EPPS is orally active and penetrates the blood-brain barrier.
- Buffering agent with a useful pH range from 7.3 to 8.7.
- Reduces Aβ-aggregate-induced memory deficits.
- Rescues cognitive deficits in mice.
- Orally active.
- Penetrates the blood-brain barrier.
Bioworld TAE Buffer, Quick-Mix Pack (25X), 1 PK(s)
Product DescriptionTAE (Tris-Acetate-EDTA) buffer quick-mix pack prepares 1 L of 25X concentration. This solution has a pH level between 8.2 – 8.4 and is stored at room temperature.TAE is used for electrophoresis of nucleic acids in agarose and polyacrylamide gels. It can be used for both genomic and large supercoiled DNA, and can also be used as both a running buffer and a gel preparation buffer. It is recommended for resolution of RNA and DNA fragments larger than 1500 bp, for genomic DNA and for large supercoiled DNA. TAE buffer has a relatively low buffering capacity and therefore replacement of the buffer during prolonged electrophoresis is recommended.
Bioworld TBE Urea Sample Buffer (2X), 25 mL
Product DescriptionTBE(Tris-Borate-EDTA) urea sample buffer is used for nucleic acid analysis. It has a 2X concentration and contains 45 mM Tris base, 45 mM Boric acid, 6% Ficol, 3.5M Urea, 0.005% Bromophenol blue, 0.025% Xylene and 1 mM EDTA.For laboratory use only. Not fit for agricultural, household, clinical use or human consumption.
Kd Medical Inc PBS Solution With Sodium Azide, 500 mL, Antimicrobial Preservative
PBS W/SODIUM AZIDE 500ML is a buffered saline solution designed for laboratory applications requiring a preservative to inhibit microbial growth. This product not only provides a stable wash and storage buffer but also enhances the longevity of biological samples. The inclusion of sodium azide ensures effective preservation, differentiating it from standard saline solutions. This versatile product is essential for researchers seeking reliable and long-lasting buffer solutions.
Sigma Aldrich Fine Chemicals Biosciences HEPBS >=99% (titration) | 161308-36-7 | MFCD00677124 | 100G
HEPBS >=99% (titration) | Purity: >=99% (titration) | Mol Wt: 266.36 | 161308-36-7 | MFCD00677124 | 100G
Sigma Aldrich Fine Chemicals Biosciences CHES BioUltra, >=99.5% (T) | 103-47-9 | MFCD00003835 | 50G
CHES BioUltra, >=99.5% (T) | Purity: >=99.5% (T) | Mol Wt: 207.29 | 103-47-9 | MFCD00003835 | 50G