Life Science Buffers
Balanced salt solutions and powders used for molecular, protein, nucleic acid, and cell biology applications including cell culture, electrophoresis, ELISA, and chromatography processes; available in a variety of formulations and grades.
Buffer solutions serve to keep pH (acidity or alkalinity) nearly constant in a variety of chemical and biological applications. For example, a bicarbonate buffer helps maintain the pH of blood, a buffered saline maintains cellular contents at a consistent pH level, and buffers help maintain a narrow pH range for enzymes to function correctly.
Most biological samples used in research are kept at a pH of about 7.4. Common biological buffers used for tissue culture include Dulbeccos phosphate buffered saline (PBS), Tris base, HEPES, MOPS, PIPES, and other formulations developed for specific cell lines or applications. Most buffers used in cell culture are also DNase-, RNase-, and protease-free.
The choice of buffer for a particular biological reaction or site depends on several factors:
- Temperature
- Desired or target pH
- Buffer toxicity (to the system)
- Buffer interactions with other system components
Ethos Biosciences Dilurex 10X PBS Buffer, 100ml, Ethos
Dilurex 10X PBS is a concentrated solution of phosphate buffered saline intended as a stock solution. Save space by keeping 10x concentrate and dilute to 1x pH 7.4 working concentration when needed by mixing 1 part 10X PBS with 9 parts distilled water. Skip the tedium of weighing powders and stocking raw materials that can expire. Our PBS is convenient, ready to use, and will save you hours in the lab, letting you focus on breakthroughs rather than buffer prep. Thousands of researchers trust Ethos Biosciences 10X PBS for its proven track record in top-tier publications and protocols - upgrade your productivity today.
![Alkali Scientific PBS [Phosphate Buffered Saline], 1X Solution, 1.0mL Pre Filled Tubes, Sterile, 1000 Tubes per Case, 2.0mL Tube](https://assets.fishersci.com/TFS-Assets/CCG/product-images/5000661558.jpg-250.jpg)
Alkali Scientific PBS [Phosphate Buffered Saline], 1X Solution, 1.0mL Pre Filled Tubes, Sterile, 1000 Tubes per Case, 2.0mL Tube
PBS (Phosphate Buffered Saline) 1X Solution is offered in a large, ready-to-use format with 1.0mL pre-filled tubes, making it suitable for laboratory applications that require rapid, high-volume processing. With 1000 tubes per case, this product is ideal for clinical laboratories, research facilities, and biotechnological applications that perform large-scale sample analysis. PBS helps maintain osmotic pressure and pH stability, which is essential for preserving cell integrity during experiments. The pre-filled tubes offer convenience and reduce contamination risks, making them ideal for use in microbiology, molecular biology, and cell culture studies where consistent buffer conditions are required.
Bioworld PBS-Casein, 0.5%, 500 mL
Supplied in PBS as 0.5% casein ready for use.pH range 7.4 (+/- 0.1)Sold as 2 x 100mL or 500mL.
Medchemexpress LLC Phosphate-Buffered Saline | 500 ML
Phosphate-Buffered Saline (PBS) is a balanced salt solution designed for various cell culture applications. These applications include washing cells, diluting cells, and preparing reagents. The standard base specification for this product is 500 mL, with larger sizes being incremental volumes of this base.
- Filtered and ready to use for general cell culture purposes.
- Free of Mg2+, Ca2+, and phenol red.
- pH is 7.2-7.4.
ILEX LIFE SCIENCES LLC TRYPSIN P10-021100 100ML
PAN-Biotech Trypsin 0.25% in PBS, w/o: Ca and Mg (100 ml). DESCRIPTION: PAN-Biotech Trypsin 0.25% in PBS, w/o: Ca and Mg is a porcine pancreas-derived enzyme in PBS solution. It is commonly used for the dissociation of anchorage-dependent (adherent) mammalian cells and tissues from culture surfaces. The concentration of trypsin necessary to dislodge cells from their substrate is dependent on the cell type and the age of the culture. APPLICATION: Enzymatic solution for the dissociation and disaggregation of anchorage-dependent mammalian cells and tissues. Trypsin solutions can range from 0.025% to 0.5% depending on application. LIQUID/POWDER: Liquid. PHENOL RED: Without Phenol Red. Ca/Mg CONTENT: Without Ca and Mg. TRYPSIN: 0.25% Trypsin. EDTA: No EDTA. STERILE: Yes. STORAGE TEMP: -20°C. MANUFACTURER: PAN-Biotech GmbH (Germany).
Teknova 1X PBS Solution plus 0.05M Sodium Chloride, pH 2.6. 1000mL, Sterile. 2 pack.
Phosphate-buffered saline (PBS) is a salt solution that is commonly used in physiological and biochemical experiments. It can be used to maintain pH neutrality or to buffer against changes in pH in order to provide optimal conditions for cellular growth and function. The buffering range is between pH 6.8 and 7.4.
Apexbio Technology LLC Annexin V-HF647/DAPI Apoptosis Kit 20assays
Annexin V is a Ca2 -dependent phospholipid-binding protein that specifically binds to phosphatidylserine (PS) Normally PS is localized on the inner leaflet of the cell membrane lipid bilayer During the early stages of apoptosis PS translocates from the inner leaflet to the outer surface of the cell membrane exposing it to the extracellular environment Due to Annexin V s high affinity for PS fluorescently labeled Annexin V (HF647) can be used as a fluorescent probe to detect apoptotic cells through flow cytometry or fluorescence microscopy with staining taking only 10-20 minutes DAPI specifically binds double-stranded DNA producing blue fluorescence and normally cannot penetrate intact cell membranes However during late apoptosis or in dead cells DAPI can pass through compromised membranes to stain the nucleus Concurrent staining with Annexin V-HF647 and DAPI allows differentiation of cells at different stages of apoptosis and identification of dead cells
Alkali Scientific CellPro™ PBS Buffer, 1X, p.H 7.2, 1L, 1 Liter, 10/Case
CellPro™ PBS Buffer, 1X pH 7.2, 1 Liter Phosphate-buffered saline (PBS) is a balanced salt solution that is used for a variety of cell culture applications, such as washing cells before dissociation, transporting cells or tissue samples, diluting cells for counting, and preparing reagents. PBS is formulated without calcium and magnesium for rinsing chelators from the culture before cell dissociation. Concentrated forms of Gibco™ PBS may require pH adjustment at the time of dilution.
![Alkali Scientific PBS 1X Solution [Phosphate Buffered Saline 1X Sterile Solution], 500 Milliliters](https://assets.fishersci.com/TFS-Assets/CCG/product-images/5000660133.jpg-250.jpg)
Alkali Scientific PBS 1X Solution [Phosphate Buffered Saline 1X Sterile Solution], 500 Milliliters
PBS (Phosphate Buffered Saline) 1X Solution is a sterile, isotonic buffer solution used in a variety of biological applications. The 500 mL volume is convenient for smaller-scale applications, including cell culture, molecular biology, and immunology experiments. PBS helps maintain the osmotic balance and pH of biological systems, preventing cell lysis or shrinkage. It is frequently used to wash cells, dilute samples, and prepare reagents for biochemical assays. PBS is an essential component in maintaining physiological conditions in in vitro studies and ensuring that cells, tissues, and biomolecules remain stable during handling and experimental procedures.
Teknova 1X PBS Solution, pH 7.4. 500mL, Sterile 8 pack.
1X PBS Solution, pH 7.4. 500mL, Sterile 8 pack.
Apexbio Technology LLC EdU Flow Cytometry Assay Kits (488) 100 tests
EdU Flow Cytometry Assay Kits (488) provides a novel method based on click chemistry specifically CuAAC (copper-catalyzed azide-alkyne cycloaddition) enabling direct measurement of DNA synthesis during the S-phase of the cell cycle The thymidine analog EdU (5-ethynyl-2 -deoxyuridine) can be incorporated directly into DNA strands during DNA synthesis The alkyne group on EdU a biologically inert moiety undergoes highly selective reaction with azide-functionalized dyes via the CuAAC reaction forming a stable 1 2 3-triazole product The unique biological groups on EdU and 6-FAM Azide allow fluorescent labeling of proliferating cell DNA after conjugation enabling imaging with low background and high detection sensitivity The EdU Flow Cytometry Assay Kits (488) use 6-FAM azide to label the DNA of proliferating cells following its reaction with EdU Samples labeled in this manner can be analyzed via flow cytometry (EXC/EMI 496/516 nm)) with green fluorescence
Biotang Inc Carbonate Buffered Saline 5X, pH 9.5. 15 mM Sodium Carbonate, 750 mM Sodium Chloride, pH 9.5. Prepared in 18.2 megohms water and filtered through 0.22 micron filter. BB-535
Carbonate Buffered Saline 5X, pH 9.5. 15 mM Sodium Carbonate, 750 mM Sodium Chloride, pH 9.5. Prepared in 18.2 megohms water and filtered through 0.22 micron filter. BB-535.
![Alkali Scientific PIPES Sodium Salt [Piperazine N,N' bis(2 ethanesulfonic acid) sodium salt], 250 Grams](https://assets.fishersci.com/TFS-Assets/CCG/product-images/5000659773.jpg-250.jpg)
Alkali Scientific PIPES Sodium Salt [Piperazine N,N' bis(2 ethanesulfonic acid) sodium salt], 250 Grams
PIPES Sodium Salt (Piperazine N,N' bis(2 ethanesulfonic acid) sodium salt) is a high-purity biochemical reagent provided in a 250G pack. It is widely used as a buffering agent in biological and biochemical assays due to its excellent stability in a wide pH range. The sodium salt form of PIPES is ideal for maintaining stable pH conditions in sensitive biological environments. It is commonly used in molecular biology, protein analysis, and enzyme assays, as it does not interfere with biological systems. PIPES Sodium Salt is also used in chromatographic applications and as a mobile phase additive in high-performance liquid chromatography (HPLC).