Life Science Buffers
Balanced salt solutions and powders used for molecular, protein, nucleic acid, and cell biology applications including cell culture, electrophoresis, ELISA, and chromatography processes; available in a variety of formulations and grades.
Buffer solutions serve to keep pH (acidity or alkalinity) nearly constant in a variety of chemical and biological applications. For example, a bicarbonate buffer helps maintain the pH of blood, a buffered saline maintains cellular contents at a consistent pH level, and buffers help maintain a narrow pH range for enzymes to function correctly.
Most biological samples used in research are kept at a pH of about 7.4. Common biological buffers used for tissue culture include Dulbeccos phosphate buffered saline (PBS), Tris base, HEPES, MOPS, PIPES, and other formulations developed for specific cell lines or applications. Most buffers used in cell culture are also DNase-, RNase-, and protease-free.
The choice of buffer for a particular biological reaction or site depends on several factors:
- Temperature
- Desired or target pH
- Buffer toxicity (to the system)
- Buffer interactions with other system components
Biotang Inc HEPES Buffer 0.5M, pH 8.0, 100ml, 500 mM HEPES (4-(2-Hydroxyethyl)piperazine-1-ethanesulfonic acid), pH 8.0 +/- 0.15. BB-2080
HEPES Buffer 0.5M, pH 8.0, 100ml, 500 mM HEPES (4-(2-Hydroxyethyl)piperazine-1-ethanesulfonic acid), pH 8.0 +/- 0.15. BB-2080.
Bioworld BES (2-(Bis(2-hydroxyethyl) amino) ethanesulfonic acid) Buffer, 0.5M, pH 7.0, 250 mL
Synonym:N,N-Bis(2-hydroxyethyl)-2-aminoethanesulfonic acid sodium salt, BES sodium saltBelongs to the class of Good buffers, as described in the research of Dr. Norman Good et al. (1966), optimized for molecular biology and biochemistry applications. The characteristics of a Good′s buffer include: pKa between 6.0 and 8.0, high solubility, non toxic, limited effect on biochemical reactions, very low absorbance between 240-700 nm, enzymatic and hydrolytic stability, minimal changes due to temperature and concentration, limited effects due to ionic or salt composition of the solution, limited interaction with mineral cations and limited permeability of biological membranes.Technical Information :Useful pH range :6.4 - 7.8pKa (25 °C) :7.1 (Free acid)Warning:For laboratory use only.Not fit for agricultural, household, clinical use or human consumption.
![eMolecules ChemScene / tert-Butyl 4-(2-hydroxyethyl)-1H-pyrazole-1-carboxylate / 100mg / 761228845 / CS-0613224 / 0.000 / 143282-05-7 / [null] / 212.249 / C10H16N2O3](https://assets.fishersci.com/TFS-Assets/CCG/product-images/502472486.JPG-250.jpg)
eMolecules ChemScene / tert-Butyl 4-(2-hydroxyethyl)-1H-pyrazole-1-carboxylate / 100mg / 761228845 / CS-0613224 / 0.000 / 143282-05-7 / [null] / 212.249 / C10H16N2O3
ChemScene / tert-Butyl 4-(2-hydroxyethyl)-1H-pyrazole-1-carboxylate / 100mg / 761228845 / CS-0613224 / 0.000 / 143282-05-7 / [null] / 212.249 / C10H16N2O3
Alkali Scientific Murashige & Skoog Medium with MES Buffer and Vitamins, 122.5 Grams of Powder, Makes 25 Liters of Solution
Murashige & Skoog Medium with MES Buffer and Vitamins (25 liters, 122.5 grams of powder) provides a high-volume solution for large-scale plant tissue culture operations. This formulation includes MES buffer, which maintains a stable pH and ensures optimal growth conditions for plant cells and tissues. The medium supports a variety of plant growth applications, including shoot multiplication, organogenesis, and regeneration. The addition of vitamins and essential nutrients promotes cell division and tissue development in controlled laboratory settings. This medium is ideal for research labs, agricultural facilities, and commercial propagation projects where large volumes of plant tissue culture medium are required for successful plant growth.
![Alkali Scientific POPSO, Free Acid, [Piperazine N N' bis(2 hydroxypropane sulfonic acid)], 100 G](https://assets.fishersci.com/TFS-Assets/CCG/product-images/5000661550.jpg-250.jpg)
Alkali Scientific POPSO, Free Acid, [Piperazine N N' bis(2 hydroxypropane sulfonic acid)], 100 G
POPSO (Free Acid) is a high-purity biochemical reagent, available in a 100g package, and is commonly used in laboratory research for buffer preparation and pH stabilization. This compound is specifically designed for use in biochemical assays and molecular biology experiments where a stable pH is critical. POPSO, also known as Piperazine N,N' bis(2 hydroxypropane sulfonic acid), is used in studies that require an effective buffering agent to maintain physiological pH conditions. It is especially useful in protein analysis, enzyme reactions, and cell culture experiments. The 100g package size is ideal for smaller-scale applications and academic research, providing an accurate and reliable reagent for laboratory procedures.
Medchemexpress LLC L-Adenosine | 3080-29-3 | 99.8% | 1 ML
L-Adenosine is a metabolically stable enantiomeric analog and a potential probe. It exhibits weakly inhibitory adenosine deaminase (ADA) activity with a Ki value of 385 μM. This compound can be utilized for research into adenosine uptake and accumulation.
- Metabolically stable enantiomeric analog.
- Potential probe for research.
- Weakly inhibits adenosine deaminase activity.
- Can be used for adenosine uptake research.
- Can be used for adenosine accumulation research.
Medchemexpress LLC HEPES hemisodium salt | 103404-87-1 | MFCD00036463 | 99.0% | 249.80 | C8H18N2O4S·1/2Na | 100 MG
HEPES hemisodium salt is a zwitterionic buffering reagent commonly used to maintain physiological pH in biochemical assays and cell-culture media. Supplied as a solid powder, it is intended for preparation of HEPES buffer solutions and includes storage and handling guidance for laboratory use.
- High buffering capacity at physiological pH (6.8-8.2).
- Available as a solid powder for convenient solution preparation.
- Reported high purity suitable for analytical and cell-culture applications.
- Stable with sealed storage at 4°C and protected from moisture.
- Provided with supporting documentation (datasheet and safety information).
![Alkali Scientific PIPES Sesquisodium Salt [Piperazine N,N' bis (2 ethanesulfonic acid) sesquisodium salt], 250 G](https://assets.fishersci.com/TFS-Assets/CCG/product-images/5000661552.jpg-250.jpg)
Alkali Scientific PIPES Sesquisodium Salt [Piperazine N,N' bis (2 ethanesulfonic acid) sesquisodium salt], 250 G
PIPES Sesquisodium Salt (Piperazine N,N' bis(2 ethanesulfonic acid) sesquisodium salt) is a high-purity reagent provided in a 250G pack. It is commonly used as a buffering agent in biochemical and laboratory applications due to its stability at a wide range of pH levels. PIPES is primarily used in the preparation of buffers for biological assays and research, as it does not interfere with biological systems. The sesquisodium salt form helps maintain the pH in both acidic and basic environments. This reagent is suitable for cell culture, enzyme assays, and other biochemical studies where precise pH control is essential. It is also utilized in electrophoresis, chromatography, and immunological testing.
Apexbio Technology LLC HEPES(Synonyms: HEPES buffer, 4-(2-Hydroxyethyl)-1-piperazineethanesulfonic acid), 250g, CAS: 7365-45-9.
HEPES (CAS 7365-45-9) is a zwitterionic sulfonic acid buffer commonly utilized in biomedical research due to its capacity to maintain physiological pH stability within a buffering range of approximately pH 6 8 to 8 2 Mechanistically HEPES modulates intracellular pH balance thereby promoting lysosomal biogenesis and supporting associated cellular processes It is routinely employed in cell culture systems to precisely regulate environmental conditions and is relevant in biochemical assays involving specific cellular signaling pathways and intracellular pH-dependent phenomena
Dojindo Molecular Technologies Inc ACES (25G), Good's buffer, CAS: 7365-82-4, pka=6.9, Active pH 6-7.5, made in Japan
In biological experiments, it is important to maintain the pH of the solutions used. Buffers, which are mixtures of weak acids and their appropriate conjugate bases, are commonly used in these experiments. Most biological reactions occur when the buffer is at its effective pH range of 6 to 8. Furthermore, the acids and bases used in the biological reaction should not produce chelates with metal ions. For these reasons, Dr. Good developed several aminoethane and aminopropane sulfonic acids that are now widely used for biological research and analysis. Goods buffers have the following characteristics: 1) High water-solubility. 2) Low cell membrane permeability. 3) Consistent acid-base dissociation constants. 4) Low metal chelating capability. 5) High chemical stability. 6) Low absorption spectra in UV and visible regions.