Life Science Buffers
Balanced salt solutions and powders used for molecular, protein, nucleic acid, and cell biology applications including cell culture, electrophoresis, ELISA, and chromatography processes; available in a variety of formulations and grades.
Buffer solutions serve to keep pH (acidity or alkalinity) nearly constant in a variety of chemical and biological applications. For example, a bicarbonate buffer helps maintain the pH of blood, a buffered saline maintains cellular contents at a consistent pH level, and buffers help maintain a narrow pH range for enzymes to function correctly.
Most biological samples used in research are kept at a pH of about 7.4. Common biological buffers used for tissue culture include Dulbeccos phosphate buffered saline (PBS), Tris base, HEPES, MOPS, PIPES, and other formulations developed for specific cell lines or applications. Most buffers used in cell culture are also DNase-, RNase-, and protease-free.
The choice of buffer for a particular biological reaction or site depends on several factors:
- Temperature
- Desired or target pH
- Buffer toxicity (to the system)
- Buffer interactions with other system components
Teknova 5X PBS-TBN Buffer. 1000mL, Sterile. 8 pack.
Phosphate-buffered saline (PBS) is a salt solution that is commonly used in physiological and biochemical experiments. It can be used to maintain pH neutrality or to buffer against changes in pH in order to provide optimal conditions for cellular growth and function. The buffering range is between pH 6.8 and 7.4.
Teknova 10 Saline Solution Sterile.
10% Saline Solution, Sterile. 10 ml Per Tube, 25 Per Pack. 4 pack.
Teknova 1M Tris-HCl, pH 8.0 Solution, 1000mL, Sterile. 2 Pack.
Tris (tris(hydroxymethyl)aminomethane) is used as a component ofbuffer solutions, especially for solutions ofnucleic acids. The useful buffer range for tris (pH 7–9) is similar to the pH of most living organisms. This, and its low cost, make tris one of the most common buffers in the biology/biochemistry laboratory.
Teknova 1M Tris-HCl, pH 8.5 Solution, 1000mL, Sterile. 2 Pack.
Tris (tris(hydroxymethyl)aminomethane) is used as a component ofbuffer solutions, especially for solutions ofnucleic acids. The useful buffer range for tris (pH 7–9) is similar to the pH of most living organisms. This, and its low cost, make tris one of the most common buffers in the biology/biochemistry laboratory.
Sigma Aldrich Fine Chemicals Biosciences Trizma acetate | 6850-28-8 | MFCD00038951 | 250g
Trizma acetate | Purity: ≥99% | Mol Wt: 181.19 | 6850-28-8 | MFCD00038951 | 250g
Research Products International Corp HEPES, Sodium Salt, 250 G
pKa (20°C): 7.45-7.65
pH Range: 6.8-8.2
Sigma Aldrich Fine Chemicals Biosciences Tris Buffered Saline, with Tween 20, pH 8.0 powder
Tris Buffered Saline, with Tween 20, pH 8.0 powder
MilliporeSigma END COVER PINCH PIPES,3/8X 5/8
NovaSeal™ End cover pinch pipes, 3/8 in. ID x 5/8 in. OD
Kd Medical Inc TRIS HCL, 1M PH 6.8, 500ML
Tris, Hydrochloride is commonly used as a buffer component for phenol extractions of DNA or RNA and a buffering component of separating and stacking gels in the characterization of protein products by SDS-PAGE. 1M solution, pH 6.8. One 500ml bottle.
Sigma Aldrich Fine Chemicals Biosciences TAPS sodium salt 99 titrat500G
TAPS sodium salt 99 titrat500G