Life Science Buffers
Balanced salt solutions and powders used for molecular, protein, nucleic acid, and cell biology applications including cell culture, electrophoresis, ELISA, and chromatography processes; available in a variety of formulations and grades.
Buffer solutions serve to keep pH (acidity or alkalinity) nearly constant in a variety of chemical and biological applications. For example, a bicarbonate buffer helps maintain the pH of blood, a buffered saline maintains cellular contents at a consistent pH level, and buffers help maintain a narrow pH range for enzymes to function correctly.
Most biological samples used in research are kept at a pH of about 7.4. Common biological buffers used for tissue culture include Dulbeccos phosphate buffered saline (PBS), Tris base, HEPES, MOPS, PIPES, and other formulations developed for specific cell lines or applications. Most buffers used in cell culture are also DNase-, RNase-, and protease-free.
The choice of buffer for a particular biological reaction or site depends on several factors:
- Temperature
- Desired or target pH
- Buffer toxicity (to the system)
- Buffer interactions with other system components
Biotang Inc HEPES Lysis Buffer with Triton (2X), 125 ml
HEPES Lysis Buffer with Triton (2X), 125 ml.
eMolecules methyl 4-((3-(hydroxymethyl)piperidin-1-yl)methyl)benzoate | 415952-27-1 | MFCD01452616 | 1g
Broadpharm | methyl 4-((3-(hydroxymethyl)piperidin-1-yl)methyl)benzoate | 1g | 112541923 | BP-20208 | 97.000 | 415952-27-1 | MFCD01452616 | 263.337 | C15H21NO3
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Ethos Biosciences Buffer Diluent phosphate concentrate 1/EA
Dilurex 1X PBS is a ready-to-use working concentration of pH 7 4 phosphate buffered saline Skip the tedium of weighing powders and stocking raw materials that can expire Our PBS is convenient ready to use and will save you hours in the lab letting you focus on breakthroughs rather than buffer prep Thousands of researchers trust Ethos Biosciences 1X PBS for its proven track record in top-tier publications and protocols - upgrade your productivity today
Boston Bioproducts Inc MES Buffer (1 M, pH 5.0) - 250ml
MES Buffer [2- (N-morpholino) ethanesulfonic acid] is a zwitterionic Good's buffer used in biological and biochemical research. It maintains a stable pH within the range of 5.5 to 7.0 and is highly soluble in water. MES is commonly used as a running buffer for bis-tris gels, chromatography, and in protein purification. It is valuable for enzymatic reactions, and acts as a non-coordinating buffer in metal ion chemistry. It has minimal UV absorbance and is stable in various applications.
FUJIFILM BIOSCIENCES INC HEPES Buffer Solution 1M
Buffer to maintain pH between 7.2 and 7.6. Suitable for use in cell cultures outside of a CO2 incubator.
Boston Bioproducts Inc Tris-HCl Buffer (1 M, pH 7.6) - 500ml
The Tris-HCl Buffer is a widely used bufering reagent in molecular biology and biochemistry, acting to maintain a stable pH, and preserve the integrity of target macromolecules. The buffering capacity of Tris-HCl is effective in the pH range of 7.0 to 9.0, making it ideal for many biological applications like cell culture, molecular biology, DNA/RNA extraction, electrophoresis, enzyme assays and immunohistostaining.