Life Science Buffers
Balanced salt solutions and powders used for molecular, protein, nucleic acid, and cell biology applications including cell culture, electrophoresis, ELISA, and chromatography processes; available in a variety of formulations and grades.
Buffer solutions serve to keep pH (acidity or alkalinity) nearly constant in a variety of chemical and biological applications. For example, a bicarbonate buffer helps maintain the pH of blood, a buffered saline maintains cellular contents at a consistent pH level, and buffers help maintain a narrow pH range for enzymes to function correctly.
Most biological samples used in research are kept at a pH of about 7.4. Common biological buffers used for tissue culture include Dulbeccos phosphate buffered saline (PBS), Tris base, HEPES, MOPS, PIPES, and other formulations developed for specific cell lines or applications. Most buffers used in cell culture are also DNase-, RNase-, and protease-free.
The choice of buffer for a particular biological reaction or site depends on several factors:
- Temperature
- Desired or target pH
- Buffer toxicity (to the system)
- Buffer interactions with other system components
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Filtered Search Results
Avantor Macron TRIS GenAR, Macron Fine Chemicals™
CAS: 77-86-1 Molecular Formula: C4H11NO3 Molecular Weight (g/mol): 121.136 InChI Key: LENZDBCJOHFCAS-UHFFFAOYSA-N PubChem CID: 6503 ChEBI: CHEBI:9754 IUPAC Name: 2-amino-2-(hydroxymethyl)propane-1,3-diol SMILES: C(C(CO)(CO)N)O
| PubChem CID | 6503 |
|---|---|
| CAS | 77-86-1 |
| Molecular Weight (g/mol) | 121.136 |
| ChEBI | CHEBI:9754 |
| SMILES | C(C(CO)(CO)N)O |
| IUPAC Name | 2-amino-2-(hydroxymethyl)propane-1,3-diol |
| InChI Key | LENZDBCJOHFCAS-UHFFFAOYSA-N |
| Molecular Formula | C4H11NO3 |
MilliporeSigma™ Tricine, OmniPur™, Calbiochem™,
CAS: 4-1-5704 Molecular Formula: C6H13NO5 Molecular Weight (g/mol): 179.17 InChI Key: SEQKRHFRPICQDD-UHFFFAOYSA-N PubChem CID: 79784 ChEBI: CHEBI:39063 IUPAC Name: 2-[[1,3-dihydroxy-2-(hydroxymethyl)propan-2-yl]amino]acetic acid SMILES: C(C(=O)O)NC(CO)(CO)CO
| PubChem CID | 79784 |
|---|---|
| CAS | 4-1-5704 |
| Molecular Weight (g/mol) | 179.17 |
| ChEBI | CHEBI:39063 |
| SMILES | C(C(=O)O)NC(CO)(CO)CO |
| IUPAC Name | 2-[[1,3-dihydroxy-2-(hydroxymethyl)propan-2-yl]amino]acetic acid |
| InChI Key | SEQKRHFRPICQDD-UHFFFAOYSA-N |
| Molecular Formula | C6H13NO5 |
Lonza™ BioWhittaker™ Phosphate Buffered Solution (1X)
Serves as a wash solution in a wide variety of laboratory applications. BioWhittaker™ Phosphate Buffered Solution (1X) Phosphate Buffered Saline is manufactured in accordance with cGMP regulations.
| Includes | No Calcium, No Magnesium |
|---|---|
| pH | 7.3 to 7.5 |
| Chemical Name or Material | Phosphate Buffered Saline (PBS) |
| Recommended Storage | 15° to 30°C |
| Sterility | Sterile |
Cytiva HBS-N Buffer, 6x200mL
Degassed and ready to use 0.01 M HEPES pH 7.4, 0.15 M NaCl. For use in Biacore C, and Biacore 3000 SPR systems
Non-distribution item offered as a customer accommodation; additional freight charges may apply.
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Sigma Aldrich Fine Chemicals Biosciences SIGMA ALDRICH FINE CHEMICALS BIOSCIENCES
NC4008915 TRIS HYDROXYMETHYL AMINOMET 1G
Non-distribution item offered as a customer accommodation; additional freight charges may apply.
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Sigma Aldrich Fine Chemicals Biosciences SIGMA ALDRICH FINE CHEMICALS BIOSCIENCES
NC4008932 TRIS BUFFER 1G
Non-distribution item offered as a customer accommodation; additional freight charges may apply.
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Alkali Scientific CellPro™ Tris Buffered Saline 20X, pH 7.4, 20L (modified)
Alkali Scientific™ CellPro™ TBS, 20X, pH 7.4, offers a sterile and concentrated Tris-buffered saline (TBS) solution, meticulously formulated to meet the demands of various molecular biology applications. With a pH of 7.4, this buffer provides optimal conditions for a wide range of biochemical and immunological assays, ensuring reliable and reproducible results. The 20X concentration allows for convenient dilution to the desired working strength, providing flexibility and efficiency in the laboratory. Sterilized to maintain purity and eliminate the risk of contamination, CellPro™ TBS is ready for immediate use in your research experiments. Trust in the quality and consistency of CellPro™ buffers to support your molecular biology endeavors, delivering accurate and precise results every time.
Non-distribution item offered as a customer accommodation; additional freight charges may apply.
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Med Vet International Hypertonic Saline 7.2% Sterile PreservatIVe Free Solution, 1000mL, Each
Sterile, preservative-free hypertonic saline solution for use in replacement therapy of sodium, chloride and water, which may become depleted in many diseases. Each 100 mL contains 7.2 grams of sodium chloride. For use in horses and cattle.
Non-distribution item offered as a customer accommodation; additional freight charges may apply.
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