Life Science Buffers

Balanced salt solutions and powders used for molecular, protein, nucleic acid, and cell biology applications including cell culture, electrophoresis, ELISA, and chromatography processes; available in a variety of formulations and grades.

Buffer solutions serve to keep pH (acidity or alkalinity) nearly constant in a variety of chemical and biological applications. For example, a bicarbonate buffer helps maintain the pH of blood, a buffered saline maintains cellular contents at a consistent pH level, and buffers help maintain a narrow pH range for enzymes to function correctly.

Most biological samples used in research are kept at a pH of about 7.4. Common biological buffers used for tissue culture include Dulbeccos phosphate buffered saline (PBS), Tris base, HEPES, MOPS, PIPES, and other formulations developed for specific cell lines or applications. Most buffers used in cell culture are also DNase-, RNase-, and protease-free.

The choice of buffer for a particular biological reaction or site depends on several factors:

Temperature
Desired or target pH
Buffer toxicity (to the system)
Buffer interactions with other system components

376 – 390 of 3,051 results

376

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PIPES, 0.5 M Buffer Solution, pH 7.5, PROMO, Thermo Scientific Chemicals

CAS: 5625-37-6 Molecular Formula: C8H18N2O6S2 Molecular Weight (g/mol): 302.36 g/mol MDL Number: MFCD00006159 InChI Key: IHPYMWDTONKSCO-UHFFFAOYSA-N PubChem CID: 79723 ChEBI: CHEBI:44933 IUPAC Name: 2-[4-(2-sulfoethyl)piperazin-1-yl]ethanesulfonic acid SMILES: C1CN(CCN1CCS(=O)(=O)O)CCS(=O)(=O)O

Pricing & Availability
Specifications

PubChem CID	79723
CAS	5625-37-6
Molecular Weight (g/mol)	302.36 g/mol
ChEBI	CHEBI:44933
MDL Number	MFCD00006159
SMILES	C1CN(CCN1CCS(=O)(=O)O)CCS(=O)(=O)O
IUPAC Name	2-[4-(2-sulfoethyl)piperazin-1-yl]ethanesulfonic acid
InChI Key	IHPYMWDTONKSCO-UHFFFAOYSA-N
Molecular Formula	C8H18N2O6S2

377

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TRIS-Buffered Saline (TBS, 10X) pH 7.6, PROMO, Thermo Scientific Chemicals

CAS: 77-86-1 Molecular Formula: C4H11NO3 Molecular Weight (g/mol): 121.14 g/mol MDL Number: MFCD00004679 InChI Key: LENZDBCJOHFCAS-UHFFFAOYSA-N PubChem CID: 6503 ChEBI: CHEBI:9754 IUPAC Name: 2-amino-2-(hydroxymethyl)propane-1,3-diol SMILES: C(C(CO)(CO)N)O

Pricing & Availability
Specifications

PubChem CID	6503
CAS	77-86-1
Molecular Weight (g/mol)	121.14 g/mol
ChEBI	CHEBI:9754
MDL Number	MFCD00004679
SMILES	C(C(CO)(CO)N)O
IUPAC Name	2-amino-2-(hydroxymethyl)propane-1,3-diol
InChI Key	LENZDBCJOHFCAS-UHFFFAOYSA-N
Molecular Formula	C4H11NO3

378

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TAE, 50X, PROMO, Thermo Scientific Chemicals

TAE is a buffer composed of Tris, acetate, and EDTA components and is used in electrophoresis of nucleic acids in agarose and polyacrylamide gels.

Pricing & Availability
Specifications

Physical Form	Liquid
Chemical Name or Material	Buffer
Concentration	50X

379

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Hanks' Balanced Salt Solution, 1X, With Calcium and Magnesium, Without Phenol Red, PROMO, Thermo Scientific Chemicals

Pricing & Availability

380

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Tris-HCl, 0.5 M Buffer Solution, pH 8.0, Low Endotoxin, PROMO, Thermo Scientific Chemicals

CAS: 1185-53-1 Molecular Formula: C4H12ClNO3 Molecular Weight (g/mol): 157.59 g/mol MDL Number: MFCD00012590 InChI Key: QKNYBSVHEMOAJP-UHFFFAOYSA-N PubChem CID: 93573 IUPAC Name: 2-amino-2-(hydroxymethyl)propane-1,3-diol;hydrochloride SMILES: C(C(CO)(CO)N)O.Cl

Pricing & Availability
Specifications

PubChem CID	93573
CAS	1185-53-1
Molecular Weight (g/mol)	157.59 g/mol
MDL Number	MFCD00012590
SMILES	C(C(CO)(CO)N)O.Cl
IUPAC Name	2-amino-2-(hydroxymethyl)propane-1,3-diol;hydrochloride
InChI Key	QKNYBSVHEMOAJP-UHFFFAOYSA-N
Molecular Formula	C4H12ClNO3

381

MP Biomedicals™ Phosphate Buffered Saline

Used in mammalian cell culture applications.

Pricing & Availability

382

MP Biomedicals™ Tricine

Tricine is for use in electrophoresis buffers.

Pricing & Availability

383

MilliporeSigma™ CAPS, ULTROL™, Grade, Calbiochem™,

CAS: 1135-40-6 Molecular Formula: C9H19NO3S Molecular Weight (g/mol): 221.32 MDL Number: MFCD00003837 InChI Key: PJWWRFATQTVXHA-UHFFFAOYSA-N Synonym: 3-(Cyclohexylamino)propanesulfonic Acid PubChem CID: 70815 IUPAC Name: 3-(cyclohexylamino)propane-1-sulfonic acid SMILES: OS(=O)(=O)CCCNC1CCCCC1

Pricing & Availability
Specifications

PubChem CID	70815
CAS	1135-40-6
Molecular Weight (g/mol)	221.32
MDL Number	MFCD00003837
SMILES	OS(=O)(=O)CCCNC1CCCCC1
Synonym	3-(Cyclohexylamino)propanesulfonic Acid
IUPAC Name	3-(cyclohexylamino)propane-1-sulfonic acid
InChI Key	PJWWRFATQTVXHA-UHFFFAOYSA-N
Molecular Formula	C9H19NO3S

384

MilliporeSigma™ TRIS Hydrochloride, OmniPur™, Calbiochem™,

CAS: 1185-53-1 Molecular Formula: C4H12ClNO3 Molecular Weight (g/mol): 157.594 InChI Key: QKNYBSVHEMOAJP-UHFFFAOYSA-N Synonym: Trizma, Tris(hydroxymethyl)aminomethane PubChem CID: 93573 IUPAC Name: 2-amino-2-(hydroxymethyl)propane-1,3-diol;hydrochloride SMILES: C(C(CO)(CO)N)O.Cl

Pricing & Availability
Specifications

PubChem CID	93573
CAS	1185-53-1
Molecular Weight (g/mol)	157.594
SMILES	C(C(CO)(CO)N)O.Cl
Synonym	Trizma, Tris(hydroxymethyl)aminomethane
IUPAC Name	2-amino-2-(hydroxymethyl)propane-1,3-diol;hydrochloride
InChI Key	QKNYBSVHEMOAJP-UHFFFAOYSA-N
Molecular Formula	C4H12ClNO3

385

Thermo Scientific Chemicals TRIS, 1.0M buffer soln., pH 7.8

CAS: 77-86-1 Molecular Formula: C4H11NO3 Molecular Weight (g/mol): 121.136 MDL Number: MFCD00004679 InChI Key: LENZDBCJOHFCAS-UHFFFAOYSA-N PubChem CID: 6503 ChEBI: CHEBI:9754 IUPAC Name: 2-amino-2-(hydroxymethyl)propane-1,3-diol SMILES: C(C(CO)(CO)N)O

Pricing & Availability
Specifications

PubChem CID	6503
CAS	77-86-1
Molecular Weight (g/mol)	121.136
ChEBI	CHEBI:9754
MDL Number	MFCD00004679
SMILES	C(C(CO)(CO)N)O
IUPAC Name	2-amino-2-(hydroxymethyl)propane-1,3-diol
InChI Key	LENZDBCJOHFCAS-UHFFFAOYSA-N
Molecular Formula	C4H11NO3

386

Thermo Scientific Chemicals TRIS-glycine native sample buffer (4X), pH 8.6

For preparation of protein samples for native gel electrophoresis

Pricing & Availability
Specifications

Color	Blue
Physical Form	Liquid
pH	8.6
Chemical Name or Material	Sample Loading Buffer
Concentration	4X
For Use With (Application)	Preparation of protein samples for native gel electrophoresis

387

Thermo Scientific Chemicals AMPSO, 98+%

CAS: 68399-79-1 Molecular Formula: C7H17NO5S Molecular Weight (g/mol): 227.28 MDL Number: MFCD00041777 InChI Key: ACERFIHBIWMFOR-UHFFFAOYNA-N Synonym: N-(1,1-Dimethyl-2-hydroxyethyl)-3-amino-2-hydroxypropanesulfonic acid PubChem CID: 100211 IUPAC Name: 2-hydroxy-3-[(1-hydroxy-2-methylpropan-2-yl)amino]propane-1-sulfonic acid SMILES: CC(C)(CO)NCC(O)CS(O)(=O)=O

Pricing & Availability
Specifications

PubChem CID	100211
CAS	68399-79-1
Molecular Weight (g/mol)	227.28
MDL Number	MFCD00041777
SMILES	CC(C)(CO)NCC(O)CS(O)(=O)=O
Synonym	N-(1,1-Dimethyl-2-hydroxyethyl)-3-amino-2-hydroxypropanesulfonic acid
IUPAC Name	2-hydroxy-3-[(1-hydroxy-2-methylpropan-2-yl)amino]propane-1-sulfonic acid
InChI Key	ACERFIHBIWMFOR-UHFFFAOYNA-N
Molecular Formula	C7H17NO5S

388

ADA, 0.2M buffer soln., pH 7.5, Thermo Scientific™

CAS: 7415-22-7 Molecular Formula: C6H9N2NaO5 Molecular Weight (g/mol): 212.137 MDL Number: MFCD00065483 InChI Key: QCGKKVWCABJQQI-UHFFFAOYSA-M PubChem CID: 23679048 IUPAC Name: sodium;2-[(2-amino-2-oxoethyl)-(carboxymethyl)amino]acetate SMILES: C(C(=O)N)N(CC(=O)O)CC(=O)[O-].[Na+]

Pricing & Availability
Specifications

PubChem CID	23679048
CAS	7415-22-7
Molecular Weight (g/mol)	212.137
MDL Number	MFCD00065483
SMILES	C(C(=O)N)N(CC(=O)O)CC(=O)[O-].[Na+]
IUPAC Name	sodium;2-[(2-amino-2-oxoethyl)-(carboxymethyl)amino]acetate
InChI Key	QCGKKVWCABJQQI-UHFFFAOYSA-M
Molecular Formula	C6H9N2NaO5

389

TRIS-buffered saline (TBS, 10X), with 1% Triton™ X-100, Thermo Scientific Chemicals

CAS: 77-86-1 Molecular Formula: C4H11NO3 Molecular Weight (g/mol): 121.136 MDL Number: MFCD00132476 InChI Key: LENZDBCJOHFCAS-UHFFFAOYSA-N PubChem CID: 6503 ChEBI: CHEBI:9754 IUPAC Name: 2-amino-2-(hydroxymethyl)propane-1,3-diol SMILES: C(C(CO)(CO)N)O

Pricing & Availability
Specifications

PubChem CID	6503
CAS	77-86-1
Molecular Weight (g/mol)	121.136
ChEBI	CHEBI:9754
MDL Number	MFCD00132476
SMILES	C(C(CO)(CO)N)O
IUPAC Name	2-amino-2-(hydroxymethyl)propane-1,3-diol
InChI Key	LENZDBCJOHFCAS-UHFFFAOYSA-N
Molecular Formula	C4H11NO3

390

Thermo Scientific Chemicals PIPES, 1.0M buffer soln., pH 7.5

CAS: 5625-37-6 Molecular Formula: C8H18N2O6S2 Molecular Weight (g/mol): 302.36 MDL Number: MFCD00006159 InChI Key: IHPYMWDTONKSCO-UHFFFAOYSA-N PubChem CID: 79723 ChEBI: CHEBI:44933 IUPAC Name: 2-[4-(2-sulfoethyl)piperazin-1-yl]ethanesulfonic acid SMILES: C1CN(CCN1CCS(=O)(=O)O)CCS(=O)(=O)O

Pricing & Availability
Specifications

PubChem CID	79723
CAS	5625-37-6
Molecular Weight (g/mol)	302.36
ChEBI	CHEBI:44933
MDL Number	MFCD00006159
SMILES	C1CN(CCN1CCS(=O)(=O)O)CCS(=O)(=O)O
IUPAC Name	2-[4-(2-sulfoethyl)piperazin-1-yl]ethanesulfonic acid
InChI Key	IHPYMWDTONKSCO-UHFFFAOYSA-N
Molecular Formula	C8H18N2O6S2

Life Science Buffers

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