Life Science Buffers
Balanced salt solutions and powders used for molecular, protein, nucleic acid, and cell biology applications including cell culture, electrophoresis, ELISA, and chromatography processes; available in a variety of formulations and grades.
Buffer solutions serve to keep pH (acidity or alkalinity) nearly constant in a variety of chemical and biological applications. For example, a bicarbonate buffer helps maintain the pH of blood, a buffered saline maintains cellular contents at a consistent pH level, and buffers help maintain a narrow pH range for enzymes to function correctly.
Most biological samples used in research are kept at a pH of about 7.4. Common biological buffers used for tissue culture include Dulbeccos phosphate buffered saline (PBS), Tris base, HEPES, MOPS, PIPES, and other formulations developed for specific cell lines or applications. Most buffers used in cell culture are also DNase-, RNase-, and protease-free.
The choice of buffer for a particular biological reaction or site depends on several factors:
- Temperature
- Desired or target pH
- Buffer toxicity (to the system)
- Buffer interactions with other system components
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Filtered Search Results
Tris, MP Biomedicals™
CAS: 77-86-1 Molecular Formula: C4H11NO3 Molecular Weight (g/mol): 121.136 InChI Key: LENZDBCJOHFCAS-UHFFFAOYSA-N Synonym: Trometamol, Tromethamine, Tris-[hydroxymethyl]aminomethane, 2-Amino-2-(hydroxymethyl)-1,3-propanediol PubChem CID: 6503 ChEBI: CHEBI:9754 IUPAC Name: 2-amino-2-(hydroxymethyl)propane-1,3-diol SMILES: C(C(CO)(CO)N)O
| PubChem CID | 6503 |
|---|---|
| CAS | 77-86-1 |
| Molecular Weight (g/mol) | 121.136 |
| ChEBI | CHEBI:9754 |
| SMILES | C(C(CO)(CO)N)O |
| Synonym | Trometamol, Tromethamine, Tris-[hydroxymethyl]aminomethane, 2-Amino-2-(hydroxymethyl)-1,3-propanediol |
| IUPAC Name | 2-amino-2-(hydroxymethyl)propane-1,3-diol |
| InChI Key | LENZDBCJOHFCAS-UHFFFAOYSA-N |
| Molecular Formula | C4H11NO3 |
MOPS-SDS Running Buffer, 20X, PROMO, Thermo Scientific Chemicals
MOPS-SDS running buffer is a buffer frequently used for separating medium- to large-sized proteins on a gel.
PIPES, 0.5 M Buffer Solution, pH 7.0, PROMO, Thermo Scientific Chemicals
CAS: 5625-37-6 Molecular Formula: C8H18N2O6S2 Molecular Weight (g/mol): 302.36 g/mol MDL Number: MFCD00006159 InChI Key: IHPYMWDTONKSCO-UHFFFAOYSA-N PubChem CID: 79723 ChEBI: CHEBI:44933 IUPAC Name: 2-[4-(2-sulfoethyl)piperazin-1-yl]ethanesulfonic acid SMILES: C1CN(CCN1CCS(=O)(=O)O)CCS(=O)(=O)O
| PubChem CID | 79723 |
|---|---|
| CAS | 5625-37-6 |
| Molecular Weight (g/mol) | 302.36 g/mol |
| ChEBI | CHEBI:44933 |
| MDL Number | MFCD00006159 |
| SMILES | C1CN(CCN1CCS(=O)(=O)O)CCS(=O)(=O)O |
| IUPAC Name | 2-[4-(2-sulfoethyl)piperazin-1-yl]ethanesulfonic acid |
| InChI Key | IHPYMWDTONKSCO-UHFFFAOYSA-N |
| Molecular Formula | C8H18N2O6S2 |
HEPES-Buffered Saline, 2X, pH 7.0, Low Endotoxin, PROMO, Thermo Scientific Chemicals
MES, 0.2 M Buffer Solution, pH 6.0, Low Endotoxin, PROMO, Thermo Scientific Chemicals
CAS: 145224-94-8 Molecular Formula: C6H15NO5S Molecular Weight (g/mol): 213.25 g/mol MDL Number: MFCD00283848 InChI Key: MIIIXQJBDGSIKL-UHFFFAOYSA-N PubChem CID: 16218417 IUPAC Name: 2-morpholin-4-ylethanesulfonic acid;hydrate SMILES: C1COCCN1CCS(=O)(=O)O.O
| PubChem CID | 16218417 |
|---|---|
| CAS | 145224-94-8 |
| Molecular Weight (g/mol) | 213.25 g/mol |
| MDL Number | MFCD00283848 |
| SMILES | C1COCCN1CCS(=O)(=O)O.O |
| IUPAC Name | 2-morpholin-4-ylethanesulfonic acid;hydrate |
| InChI Key | MIIIXQJBDGSIKL-UHFFFAOYSA-N |
| Molecular Formula | C6H15NO5S |
MP Biomedicals™ MOPS SDS Running Buffer for Bis-Tris Gels, Powder
Powder MOPS buffer salts to make PAGE buffer.
Thermo Scientific Chemicals TRIS, 1.0M buffer soln., pH 7.2
CAS: 77-86-1 Molecular Formula: C4H11NO3 Molecular Weight (g/mol): 121.136 MDL Number: MFCD00004679 InChI Key: LENZDBCJOHFCAS-UHFFFAOYSA-N PubChem CID: 6503 ChEBI: CHEBI:9754 IUPAC Name: 2-amino-2-(hydroxymethyl)propane-1,3-diol SMILES: C(C(CO)(CO)N)O
| PubChem CID | 6503 |
|---|---|
| CAS | 77-86-1 |
| Molecular Weight (g/mol) | 121.136 |
| ChEBI | CHEBI:9754 |
| MDL Number | MFCD00004679 |
| SMILES | C(C(CO)(CO)N)O |
| IUPAC Name | 2-amino-2-(hydroxymethyl)propane-1,3-diol |
| InChI Key | LENZDBCJOHFCAS-UHFFFAOYSA-N |
| Molecular Formula | C4H11NO3 |
| Name Note | non-reducing |
|---|---|
| Health Hazard 3 | GHS P Statement P280-P264-P305+P351+P338-P337+P313 Wear protective gloves/protective clothing/eye protection/face protection. Wash thoroughly after handling. IF IN EYES: Rinse cautiously with water for several minutes. Remove contact lenses, if present and easy to do. Continue rinsing. If eye irritation persists: Get medical advice/attention. |
| Health Hazard 2 | GHS H Statement H319 Causes serious eye irritation. |
| Color | Blue |
| Physical Form | Liquid |
| Health Hazard 1 | H335 |
| Chemical Name or Material | Tricine-SDS Sample Buffer |
| TSCA | Yes |
| Recommended Storage | Ambient temperatures |
| Concentration | 2X |
Tricine, 0.5M buffer soln., pH 7.5, Thermo Scientific™
CAS: 5704-04-1 Molecular Formula: C6H13NO5 Molecular Weight (g/mol): 179.17 MDL Number: MFCD00004277 InChI Key: SEQKRHFRPICQDD-UHFFFAOYSA-N PubChem CID: 79784 ChEBI: CHEBI:39063 IUPAC Name: 2-[[1,3-dihydroxy-2-(hydroxymethyl)propan-2-yl]amino]acetic acid SMILES: C(C(=O)O)NC(CO)(CO)CO
| PubChem CID | 79784 |
|---|---|
| CAS | 5704-04-1 |
| Molecular Weight (g/mol) | 179.17 |
| ChEBI | CHEBI:39063 |
| MDL Number | MFCD00004277 |
| SMILES | C(C(=O)O)NC(CO)(CO)CO |
| IUPAC Name | 2-[[1,3-dihydroxy-2-(hydroxymethyl)propan-2-yl]amino]acetic acid |
| InChI Key | SEQKRHFRPICQDD-UHFFFAOYSA-N |
| Molecular Formula | C6H13NO5 |
Thermo Scientific Chemicals MOPS, 0.5M buffer soln., pH 7.5
CAS: 1132-61-2 Molecular Formula: C7H15NO4S Molecular Weight (g/mol): 209.26 MDL Number: MFCD00006183 InChI Key: DVLFYONBTKHTER-UHFFFAOYSA-N PubChem CID: 70807 ChEBI: CHEBI:44115 IUPAC Name: 3-morpholin-4-ylpropane-1-sulfonic acid SMILES: [O-]S(=O)(=O)CCC[NH+]1CCOCC1
| PubChem CID | 70807 |
|---|---|
| CAS | 1132-61-2 |
| Molecular Weight (g/mol) | 209.26 |
| ChEBI | CHEBI:44115 |
| MDL Number | MFCD00006183 |
| SMILES | [O-]S(=O)(=O)CCC[NH+]1CCOCC1 |
| IUPAC Name | 3-morpholin-4-ylpropane-1-sulfonic acid |
| InChI Key | DVLFYONBTKHTER-UHFFFAOYSA-N |
| Molecular Formula | C7H15NO4S |
Enzyme storage buffer in PBS and glycerol, autoclaved, Thermo Scientific™
For frozen storage of enzymes
| Physical Form | Liquid |
|---|---|
| Chemical Name or Material | Enzyme storage buffer in PBS and glycerol |
| Recommended Storage | Ambient temperatures |
| Color | Colorless |
|---|---|
| Physical Form | Liquid |
| pH | 8.0 |
| Recommended Storage | Keep cold |
| Odor | Odorless |
| Health Hazard 3 | P264b-P280i-P305+P351+P338 |
|---|---|
| Health Hazard 2 | GHS H Statement H319 Causes serious eye irritation. |
| Color | Colorless |
| Physical Form | Liquid |
| Health Hazard 1 | H319 |
| Chemical Name or Material | HEPES protein extraction buffer |
| TSCA | Yes |
| Recommended Storage | Ambient temperatures |
| Concentration | 5X |