Life Science Buffers
Balanced salt solutions and powders used for molecular, protein, nucleic acid, and cell biology applications including cell culture, electrophoresis, ELISA, and chromatography processes; available in a variety of formulations and grades.
Buffer solutions serve to keep pH (acidity or alkalinity) nearly constant in a variety of chemical and biological applications. For example, a bicarbonate buffer helps maintain the pH of blood, a buffered saline maintains cellular contents at a consistent pH level, and buffers help maintain a narrow pH range for enzymes to function correctly.
Most biological samples used in research are kept at a pH of about 7.4. Common biological buffers used for tissue culture include Dulbeccos phosphate buffered saline (PBS), Tris base, HEPES, MOPS, PIPES, and other formulations developed for specific cell lines or applications. Most buffers used in cell culture are also DNase-, RNase-, and protease-free.
The choice of buffer for a particular biological reaction or site depends on several factors:
- Temperature
- Desired or target pH
- Buffer toxicity (to the system)
- Buffer interactions with other system components
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Filtered Search Results
MilliporeSigma™ Calbiochem™ OmniPur™ 100X TE Buffer Solution Liquid Concentrate
For use in molecular biology applications
MilliporeSigma™ Tris Buffer, 100mM, pH 7.4, Molecular Biology Grade, Calbiochem™,
CAS: 77-86-1 Molecular Formula: C4H11NO3 Molecular Weight (g/mol): 121.136 InChI Key: LENZDBCJOHFCAS-UHFFFAOYSA-N Synonym: tris(Hydroxymethyl)aminomethane, HCl PubChem CID: 6503 ChEBI: CHEBI:9754 IUPAC Name: 2-amino-2-(hydroxymethyl)propane-1,3-diol SMILES: C(C(CO)(CO)N)O
| PubChem CID | 6503 |
|---|---|
| CAS | 77-86-1 |
| Molecular Weight (g/mol) | 121.136 |
| ChEBI | CHEBI:9754 |
| SMILES | C(C(CO)(CO)N)O |
| Synonym | tris(Hydroxymethyl)aminomethane, HCl |
| IUPAC Name | 2-amino-2-(hydroxymethyl)propane-1,3-diol |
| InChI Key | LENZDBCJOHFCAS-UHFFFAOYSA-N |
| Molecular Formula | C4H11NO3 |
TBS, 20X Solution, pH 7.4, Molecular Biology Grade, Ultrapure
CAS: 7647-14-5 Molecular Formula: ClNa Molecular Weight (g/mol): 58.44 MDL Number: MFCD00003477 InChI Key: FAPWRFPIFSIZLT-UHFFFAOYSA-M PubChem CID: 5234 ChEBI: CHEBI:26710 IUPAC Name: sodium;chloride SMILES: [Na+].[Cl-]
| PubChem CID | 5234 |
|---|---|
| CAS | 7647-14-5 |
| Molecular Weight (g/mol) | 58.44 |
| ChEBI | CHEBI:26710 |
| MDL Number | MFCD00003477 |
| SMILES | [Na+].[Cl-] |
| IUPAC Name | sodium;chloride |
| InChI Key | FAPWRFPIFSIZLT-UHFFFAOYSA-M |
| Molecular Formula | ClNa |
MP Biomedicals™ MES SDS Running Buffer for Bis-Tris Gels, Powder
Powder MES buffer salts to make PAGE buffer.
MilliporeSigma™ HEPES, Free Acid, ULTROL™ Grade, Calbiochem™,
CAS: 7365-45-9 Molecular Formula: C8H18N2O4S Molecular Weight (g/mol): 238.30 MDL Number: MFCD00006158 InChI Key: JKMHFZQWWAIEOD-UHFFFAOYSA-N Synonym: N-2-Hydroxyethylpiperazine-N'-2-ethanesulfonic Acid PubChem CID: 23831 ChEBI: CHEBI:42334 IUPAC Name: 2-[4-(2-hydroxyethyl)piperazin-1-yl]ethane-1-sulfonic acid SMILES: OCCN1CCN(CCS(O)(=O)=O)CC1
| PubChem CID | 23831 |
|---|---|
| CAS | 7365-45-9 |
| Molecular Weight (g/mol) | 238.30 |
| ChEBI | CHEBI:42334 |
| MDL Number | MFCD00006158 |
| SMILES | OCCN1CCN(CCS(O)(=O)=O)CC1 |
| Synonym | N-2-Hydroxyethylpiperazine-N'-2-ethanesulfonic Acid |
| IUPAC Name | 2-[4-(2-hydroxyethyl)piperazin-1-yl]ethane-1-sulfonic acid |
| InChI Key | JKMHFZQWWAIEOD-UHFFFAOYSA-N |
| Molecular Formula | C8H18N2O4S |
Bicine, MP Biomedicals
CAS: 150-25-4 Molecular Formula: C6H12NNaO4 Molecular Weight (g/mol): 185.16 MDL Number: MFCD00004295 InChI Key: MFBDBXAVPLFMNJ-UHFFFAOYSA-M Synonym: bicine,n,n-bis 2-hydroxyethyl glycine,diethylolglycine,dihydroxyethylglycine,diethanol glycine,n,n-di 2-hydroxyethyl glycine,bicene,2-bis 2-hydroxyethyl amino acetic acid,n,n-dihydroxyethyl glycine,n,n-dihydroxyethylglycine PubChem CID: 8761 ChEBI: CHEBI:40957 IUPAC Name: 2-[bis(2-hydroxyethyl)amino]acetic acid SMILES: [Na+].OCCN(CCO)CC([O-])=O
| PubChem CID | 8761 |
|---|---|
| CAS | 150-25-4 |
| Molecular Weight (g/mol) | 185.16 |
| ChEBI | CHEBI:40957 |
| MDL Number | MFCD00004295 |
| SMILES | [Na+].OCCN(CCO)CC([O-])=O |
| Synonym | bicine,n,n-bis 2-hydroxyethyl glycine,diethylolglycine,dihydroxyethylglycine,diethanol glycine,n,n-di 2-hydroxyethyl glycine,bicene,2-bis 2-hydroxyethyl amino acetic acid,n,n-dihydroxyethyl glycine,n,n-dihydroxyethylglycine |
| IUPAC Name | 2-[bis(2-hydroxyethyl)amino]acetic acid |
| InChI Key | MFBDBXAVPLFMNJ-UHFFFAOYSA-M |
| Molecular Formula | C6H12NNaO4 |
Thermo Scientific Chemicals ACES, 99+%, for biochemistry
CAS: 7365-82-4 Molecular Formula: C4H10N2O4S Molecular Weight (g/mol): 182.194 MDL Number: MFCD00008030 InChI Key: DBXNUXBLKRLWFA-UHFFFAOYSA-N Synonym: N-(2-Acetamido)-2-aminoethanesulfonic acid,N-(Carbamoylmethyl)taurine PubChem CID: 81832 ChEBI: CHEBI:39062 IUPAC Name: 2-[(2-amino-2-oxoethyl)amino]ethanesulfonic acid SMILES: C(CS(=O)(=O)O)NCC(=O)N
| PubChem CID | 81832 |
|---|---|
| CAS | 7365-82-4 |
| Molecular Weight (g/mol) | 182.194 |
| ChEBI | CHEBI:39062 |
| MDL Number | MFCD00008030 |
| SMILES | C(CS(=O)(=O)O)NCC(=O)N |
| Synonym | N-(2-Acetamido)-2-aminoethanesulfonic acid,N-(Carbamoylmethyl)taurine |
| IUPAC Name | 2-[(2-amino-2-oxoethyl)amino]ethanesulfonic acid |
| InChI Key | DBXNUXBLKRLWFA-UHFFFAOYSA-N |
| Molecular Formula | C4H10N2O4S |
Thermo Scientific Chemicals HEPES sodium salt, 99%
CAS: 75277-39-3 Molecular Formula: C8H17N2NaO4S Molecular Weight (g/mol): 260.28 MDL Number: MFCD00036463 InChI Key: RDZTWEVXRGYCFV-UHFFFAOYSA-M Synonym: 2-[4-(2-Hydroxyethyl)-1-piperazine]ethanesulfonic acid sodium salt PubChem CID: 2724248 ChEBI: CHEBI:46758 IUPAC Name: sodium 2-[4-(2-hydroxyethyl)piperazin-1-yl]ethane-1-sulfonate SMILES: [Na+].OCCN1CCN(CCS([O-])(=O)=O)CC1
| PubChem CID | 2724248 |
|---|---|
| CAS | 75277-39-3 |
| Molecular Weight (g/mol) | 260.28 |
| ChEBI | CHEBI:46758 |
| MDL Number | MFCD00036463 |
| SMILES | [Na+].OCCN1CCN(CCS([O-])(=O)=O)CC1 |
| Synonym | 2-[4-(2-Hydroxyethyl)-1-piperazine]ethanesulfonic acid sodium salt |
| IUPAC Name | sodium 2-[4-(2-hydroxyethyl)piperazin-1-yl]ethane-1-sulfonate |
| InChI Key | RDZTWEVXRGYCFV-UHFFFAOYSA-M |
| Molecular Formula | C8H17N2NaO4S |
Tris, 99.8 to 100.1% (Dry Basis), Molecular Biology Grade, Ultrapure
CAS: 77-86-1 Molecular Formula: C4H11NO3 Molecular Weight (g/mol): 121.136 MDL Number: MFCD00004679 InChI Key: LENZDBCJOHFCAS-UHFFFAOYSA-N Synonym: Tris(hydroxymethyl)amino methane PubChem CID: 6503 ChEBI: CHEBI:9754 IUPAC Name: 2-amino-2-(hydroxymethyl)propane-1,3-diol SMILES: C(C(CO)(CO)N)O
| PubChem CID | 6503 |
|---|---|
| CAS | 77-86-1 |
| Molecular Weight (g/mol) | 121.136 |
| ChEBI | CHEBI:9754 |
| MDL Number | MFCD00004679 |
| SMILES | C(C(CO)(CO)N)O |
| Synonym | Tris(hydroxymethyl)amino methane |
| IUPAC Name | 2-amino-2-(hydroxymethyl)propane-1,3-diol |
| InChI Key | LENZDBCJOHFCAS-UHFFFAOYSA-N |
| Molecular Formula | C4H11NO3 |
TRIS, MP Biomedicals™
CAS: 77-86-1 Molecular Formula: C4H11NO3 Molecular Weight (g/mol): 121.14 InChI Key: LENZDBCJOHFCAS-UHFFFAOYSA-N Synonym: 2-Amino-2-(hydroxymethyl)-1,3-propanediol,THAM, Tris-(hydroxymethyl) aminomethane,Trometamol
| CAS | 77-86-1 |
|---|---|
| Molecular Weight (g/mol) | 121.14 |
| Synonym | 2-Amino-2-(hydroxymethyl)-1,3-propanediol,THAM, Tris-(hydroxymethyl) aminomethane,Trometamol |
| InChI Key | LENZDBCJOHFCAS-UHFFFAOYSA-N |
| Molecular Formula | C4H11NO3 |
MilliporeSigma™ Bicine, ULTROL™ Grade, Calbiochem™,
CAS: 150-25-4 Molecular Formula: C6H12NNaO4 Molecular Weight (g/mol): 185.16 MDL Number: MFCD00004295 InChI Key: MFBDBXAVPLFMNJ-UHFFFAOYSA-M Synonym: N,N-bis-(2-Hydroxyethyl)glycine PubChem CID: 8761 ChEBI: CHEBI:40957 IUPAC Name: sodium 2-[bis(2-hydroxyethyl)amino]acetate SMILES: [Na+].OCCN(CCO)CC([O-])=O
| PubChem CID | 8761 |
|---|---|
| CAS | 150-25-4 |
| Molecular Weight (g/mol) | 185.16 |
| ChEBI | CHEBI:40957 |
| MDL Number | MFCD00004295 |
| SMILES | [Na+].OCCN(CCO)CC([O-])=O |
| Synonym | N,N-bis-(2-Hydroxyethyl)glycine |
| IUPAC Name | sodium 2-[bis(2-hydroxyethyl)amino]acetate |
| InChI Key | MFBDBXAVPLFMNJ-UHFFFAOYSA-M |
| Molecular Formula | C6H12NNaO4 |
MilliporeSigma™ HEPES, Free Acid, ULTROL™ Grade, 1.0 M Solution, Calbiochem™,
CAS: 7365-45-9 Molecular Formula: C8H18N2O4S Molecular Weight (g/mol): 238.30 MDL Number: MFCD00006158 InChI Key: JKMHFZQWWAIEOD-UHFFFAOYSA-N PubChem CID: 23831 ChEBI: CHEBI:42334 IUPAC Name: 2-[4-(2-hydroxyethyl)piperazin-1-yl]ethane-1-sulfonic acid SMILES: OCCN1CCN(CCS(O)(=O)=O)CC1
| PubChem CID | 23831 |
|---|---|
| CAS | 7365-45-9 |
| Molecular Weight (g/mol) | 238.30 |
| ChEBI | CHEBI:42334 |
| MDL Number | MFCD00006158 |
| SMILES | OCCN1CCN(CCS(O)(=O)=O)CC1 |
| IUPAC Name | 2-[4-(2-hydroxyethyl)piperazin-1-yl]ethane-1-sulfonic acid |
| InChI Key | JKMHFZQWWAIEOD-UHFFFAOYSA-N |
| Molecular Formula | C8H18N2O4S |
R&D Systems™ 10X PBS
The 10X PBS (phosphate buffered saline) buffer is a high quality concentrated solution manufactured without magnesium or calcium, and is suitable for cell resuspension and washing in the in situ apoptosis detection (TUNEL) procedure, CometAssay™ (Cat No. 4250-50-K) applications, and any other experiment requiring ultrapure PBS buffer.