Life Science Buffers
Balanced salt solutions and powders used for molecular, protein, nucleic acid, and cell biology applications including cell culture, electrophoresis, ELISA, and chromatography processes; available in a variety of formulations and grades.
Buffer solutions serve to keep pH (acidity or alkalinity) nearly constant in a variety of chemical and biological applications. For example, a bicarbonate buffer helps maintain the pH of blood, a buffered saline maintains cellular contents at a consistent pH level, and buffers help maintain a narrow pH range for enzymes to function correctly.
Most biological samples used in research are kept at a pH of about 7.4. Common biological buffers used for tissue culture include Dulbeccos phosphate buffered saline (PBS), Tris base, HEPES, MOPS, PIPES, and other formulations developed for specific cell lines or applications. Most buffers used in cell culture are also DNase-, RNase-, and protease-free.
The choice of buffer for a particular biological reaction or site depends on several factors:
- Temperature
- Desired or target pH
- Buffer toxicity (to the system)
- Buffer interactions with other system components
Boston Bioproducts Inc EDTA (0.5 M, pH 7.4) - 500ml
EDTA [Ethylenediaminetetraacetic acid] solution is commonly used in various molecular biology and protein biochemistry applications that require the chelation of divalent metal cations, such as calcium (Ca2+) and magnesium (Mg2+). It is not soluble in water unless pH is adjusted accordingly. EDTA is used extensively as an anticoagulant in blood samples as it chelates the calcium present, preventing coagulation and preserving blood cell morphology. It is also used in cell lysis buffers.
Sigma Aldrich Fine Chemicals Biosciences Phosphate buffered saline powder, pH 7.4, for preparing 1 L solutions | MFCD00131855 | 1PAK
Phosphate buffered saline powder, pH 7.4, for preparing 1 L solutions | MFCD00131855 | 1PAK
Boston Bioproducts Inc Tris-Acetate-EDTA Buffer, TAE (50X) - 1L
The Tris-Acetate-EDTA (TAE) Buffer is a pH regulator and chelator often used in molecular biology techniques such as agarose gel electrophoresis. Supporting a pH range of 8.1-8.4, the TAE Buffer combines tris base, acetic acid, and EDTA (Ethylenediaminetetraacetic Acid) to form a solution efficient in separating nucleic acids as a running buffer, or as a component of agarose gels. This Buffer is prepared in a 50X stock solution, and can be diluted to ideal concentrations.
Boston Bioproducts Inc Tris Buffered Saline, TBS (20X) - 1L
The Tris Buffered Saline (TBS) is an isotonic solution frequently used in biological research due to its non-toxic nature. The formulation of TBS includes Tris (tris(hydroxymethyl)aminomethane), which serves as a buffering agent, and Sodium Chloride (NaCl) salt. Tris maintains a stable pH range typically between 7.4 to 8.0 while NaCl regulates osmotic balance via its elected concentration. This TBS Solution is formulated at a 20X concentration, and is useful for washing in Western Blotting.
Boston Bioproducts Inc HEPES Buffer (1 M, pH 7.6, pH Adjusted with KOH) - 250ml
The HEPES [4-(2-hydroxyethyl)-1-piperazineethanesulfonic acid)] Buffer is a versatile Good's zwitterionic buffering agent widely used in various applications in biochemistry and molecular biology. It maintains a stable pH in the range of 6.8 to 8.2, making it ideal for various applications at physiological pH, including cell culture, protein purification, isoelectric focusing, enzyme studies, bioprocessing, and live cell imaging. This buffer is adjusted with KOH.
TRIBIOSCIENCE INC Inoue Transformation Buffer, 100ML
Inoue Transformation Buffer is used for the preparation of high-efficient competent cells. This product is based on the Inoue method for the preparation and transformation of E. Coli component cells.
Complement Technology Inc GVBE (GVBo with EDTA) 250ml
GVBE is an isotonic buffer used in complement assays to prevent complement activation or to stop ongoing complement activation when added at the end of an incubation
Cliawaived Inc Transport Tube, 3mL Phosphate Based Saline PBS (400 swabs and PBS Tubes per box)
Transport Tube, 3mL Phosphate Based Saline PBS. 102mm length vial contains 3ml of PBS solution. Includes mini-flocked swab with synthetic fibers and plastic shaft and break point at 80mm. INTENDED USEThe KaiBiLiTM Extended ViralTrans PBS is phosphate buffer saline which can maintain pH and osmotic balance, as well as provide cells with water and essential inorganic ions.SUMMARYAppropriate specimen collection and transport is important to accurate laboratory diagnosis of infectious disease. Not only personnel operating skills, but also a proper specimen collection and transport system are essential attributes for reliable diagnosis result. The KaiBiLiTM Extended ViralTrans PBS is suitable for collection, transport of clinical specimens.
Boston Bioproducts Inc Tris-Borate-EDTA Buffer (10X) - 4L
The Tris-Borate-EDTA (TBE) Buffer is a pH regulator and chelator used in molecular biology techniques such as nucleic acid gel electrophoresis. Supporting a pH range between 8.1 and 8.5, the TBE Buffer combines aqueous tris base, boric acid, and EDTA (Ethylenediaminetetraacetic Acid), making it efficient as a running buffer or component of agarose gels. This product is prepared in a 10X stock concentration, and can be diluted to concentrations ideal to specific laboratory workflows.
Boston Bioproducts Inc Tris-HCl Buffer (1 M, pH 9.0) - 1L
The Tris-HCl Buffer is a widely used bufering reagent in molecular biology and biochemistry, acting to maintain a stable pH, and preserve the integrity of target macromolecules. The buffering capacity of Tris-HCl is effective in the pH range of 7.0 to 9.0, making it ideal for many biological applications like cell culture, molecular biology, DNA/RNA extraction, electrophoresis, enzyme assays and immunohistostaining.
Sciencell Research Laboratories DULBECCO'S PHOSPHATE-BUFFERED
Dulbecco's Phosphate-Buffered Saline 500 ml