Life Science Buffers
Balanced salt solutions and powders used for molecular, protein, nucleic acid, and cell biology applications including cell culture, electrophoresis, ELISA, and chromatography processes; available in a variety of formulations and grades.
Buffer solutions serve to keep pH (acidity or alkalinity) nearly constant in a variety of chemical and biological applications. For example, a bicarbonate buffer helps maintain the pH of blood, a buffered saline maintains cellular contents at a consistent pH level, and buffers help maintain a narrow pH range for enzymes to function correctly.
Most biological samples used in research are kept at a pH of about 7.4. Common biological buffers used for tissue culture include Dulbeccos phosphate buffered saline (PBS), Tris base, HEPES, MOPS, PIPES, and other formulations developed for specific cell lines or applications. Most buffers used in cell culture are also DNase-, RNase-, and protease-free.
The choice of buffer for a particular biological reaction or site depends on several factors:
- Temperature
- Desired or target pH
- Buffer toxicity (to the system)
- Buffer interactions with other system components
Phoenix Flow Systems Inc CHEAP SHEATH PBS CONCENTRATE
Cheap Sheath Is A Concentrated Ph Balanced Formulation Of Phosphate Buffered Saline Which Has Been Pre-Filtered And Is Ready To Use In Any Flow Cytometer As Your Sheath Solution Upon Dilution With Distilled Water. Each Bottle Of Cheap Sheath Contains Enough Concentrate To Prepare 20 Liters Of Flow Cytometer Sheath Soluton. Formulation Is Simple For Flow Cytometers That Do Not Use 20 Liter Sheath Containers. For Any Flow Cytometer With A 3.5 Liter Sheath Tank, Add 100 Mls Cheap Sheath To 2.9 Liters Of Distilled Water In The Sheath Tank. 1Qt Bottle (2 Lbs).
Asuragen Inc TSMIII Storage Buffer, 50 mL, 10 mM Tris, 100 mM NaCl, 1 mM MgCl2, 0.1% Gelatin, 0.3% Microcide III, pH 7.0, -15 to -30°C
Asuragen TSMIII Storage Buffer is a specialized buffered solution designed to maintain stability for protein-coated nucleic acids. This reagent is ideal for the dilution of Asuragen's Armored products, ensuring the integrity of these sensitive biological materials through multiple freeze-thaw cycles. The stability provided by this storage buffer allows for flexible and scalable testing processes in various molecular assay applications.
- Volume: 50 mL
- Composition: 10 mM Tris, 100 mM NaCl, 1 mM MgCl2, 0.1% Gelatin, 0.3% Microcide III
- pH: 7.0
- Primary application: Dilution of Asuragen's Armored products
- Key benefit: Provides stability to protein-coated nucleic acids
- Storage stability: Withstands multiple freeze-thaw cycles
- Recommended storage temperature: -15 to -30 °C (secondary)
- Testing process benefit: Enables flexible and scalable testing processes
Zymo Research Corporation DNA Wash Buffer (Concentrate), 24 ml
This ensures high purity DNA to be recovered. Ethanol must be added to the concentrate prior to use. Instructions for each DNA Wash Buffer (concentrate) size is listed on the bottle and within the Zymo Research protocols it is used in.
Boston Bioproducts Inc Tris-HCl Buffer (1 M, pH 9.0) - 1L
The Tris-HCl Buffer is a widely used bufering reagent in molecular biology and biochemistry, acting to maintain a stable pH, and preserve the integrity of target macromolecules. The buffering capacity of Tris-HCl is effective in the pH range of 7.0 to 9.0, making it ideal for many biological applications like cell culture, molecular biology, DNA/RNA extraction, electrophoresis, enzyme assays and immunohistostaining.
Sigma Aldrich Fine Chemicals Biosciences Phosphate buffered saline powder, pH 7.4, for preparing 1 L solutions | MFCD00131855 | 1PAK
Phosphate buffered saline powder, pH 7.4, for preparing 1 L solutions | MFCD00131855 | 1PAK
Gold Biotechnology Inc HEPES, Sodium Salt 500 g
HEPES sodium salt is a buffering agent used in biochemistry and molecular biology that was selected and described by Good et al. It is a zwitterionic, piperazinic buffer that is useful for a pH range of 6.8 – 8.2. HEPES lacks the ability to form a significant complex with most metal ions and is recommended for use as a non-coordinating buffer in solutions with metal ions. It is commonly used in cell culture media and as an ampholytic separator to create a pH gradient in isoelectric focusing. HEPES interferes with reactions between DNA and restriction enzymes to a lesser extent than similar buffers with less substituted amine groups, such as Tris. This lack of interference occurs because steric hindrance of the protonated tertiary amine in HEPES shields the negative charge of DNA to a lesser extent than less substituted buffers. This buffer is capable of forming radicals and is therefore not suitable for redox reactions. It is suitable for use in the bicinchoninic acid (BCA) assay.
Andwin Scientific TRIS 1M ST SOLUTION PH9 250ML
Tris 1.0 M Sterile Solution, pH 9.0, Ultra Pure Grade, 250ml
Sigma Aldrich Fine Chemicals Biosciences Trizma base, 77-86-1, MFCD00004679, 100 g
Linear Formula: NH2C(CH2OH)3, Molecular Weight: 121.14, BioPerformance Certified, meets EP, USP testing specifications, cell culture tested, ≥99.9% (titration), Synonym: 2-Amino-2-(hydroxymethyl)-1,3-propanediol, THAM, Tris base, Tris(hydroxymethyl)aminomethane, Trometamol.
Sigma Aldrich Fine Chemicals Biosciences Phosphate buffered saline | MFCD00131855 | 100/pk
Phosphate buffered saline | MFCD00131855 | 100/pk