Life Science Buffers
Balanced salt solutions and powders used for molecular, protein, nucleic acid, and cell biology applications including cell culture, electrophoresis, ELISA, and chromatography processes; available in a variety of formulations and grades.
Buffer solutions serve to keep pH (acidity or alkalinity) nearly constant in a variety of chemical and biological applications. For example, a bicarbonate buffer helps maintain the pH of blood, a buffered saline maintains cellular contents at a consistent pH level, and buffers help maintain a narrow pH range for enzymes to function correctly.
Most biological samples used in research are kept at a pH of about 7.4. Common biological buffers used for tissue culture include Dulbeccos phosphate buffered saline (PBS), Tris base, HEPES, MOPS, PIPES, and other formulations developed for specific cell lines or applications. Most buffers used in cell culture are also DNase-, RNase-, and protease-free.
The choice of buffer for a particular biological reaction or site depends on several factors:
- Temperature
- Desired or target pH
- Buffer toxicity (to the system)
- Buffer interactions with other system components
Teknova 20X HBS-EP+ Buffer, pH 7.6.1000mL, Sterile.
3M Sodium Chloride, 200mM HEPES Free Acid,60mM EDTA, 1.0% Tween-20
Boca Scientific Inc Antibody Stabilizer PBS Base, 50ml
Antibody Stabilizer conserves the structure of proteins and antibodies to prevent loss of functionality due to storage. With refrigerated storage, Antibody Stabilizer prevents the need for freezing and thawing. Both TRIS-based and PBS-based Antibody Stabilizers are available, allowing the user to select the optimal buffer base for the assay.
Boston Bioproducts Inc MES Buffer (1 M, pH 6.5) - 500ml
MES Buffer [2- (N-morpholino) ethanesulfonic acid] is a zwitterionic Good's buffer used in biological and biochemical research. It maintains a stable pH within the range of 5.5 to 7.0 and is highly soluble in water. MES is commonly used as a running buffer for bis-tris gels, chromatography, and in protein purification. It is valuable for enzymatic reactions, and acts as a non-coordinating buffer in metal ion chemistry. It has minimal UV absorbance and is stable in various applications.
Boston Bioproducts Inc Tris-HCl Buffer (1 M, pH 7.4) - 500ml
The Tris-HCl Buffer is a widely used bufering reagent in molecular biology and biochemistry, acting to maintain a stable pH, and preserve the integrity of target macromolecules. The buffering capacity of Tris-HCl is effective in the pH range of 7.0 to 9.0, making it ideal for many biological applications like cell culture, molecular biology, DNA/RNA extraction, electrophoresis, enzyme assays and immunohistostaining.
Meso Scale Discovery MSD GOLD Read Buffer B | 200mL
MSD GOLD Read Buffer B, Read buffer is an essential component of MSD immunoassays: it is required for the generation of electrochemiluminescence (ECL) signals. MSD GOLD Read Buffer B was optimized for use in all MSD assays and can be used as a replacement for MSD GOLD Read Buffer A and MSD Read Buffer T (4x). MSD GOLD Read Buffer B is also recommended for assays that measure whole cell, vesicles, or other membrane-containing analytes. MSD GOLD Read Buffer B has stringent quality control release criteria that ensure lot-to-lot reproducibility. The specifications are designed to meet the requirements of critical assays and longitudinal studies.
Bioworld HEPES, 1M, pH 7.4, Sterile, 250 mL
HEPES BufferAn N-substituted aminosulfonic acid buffer with an optimal buffering pH range of 6.0 to 8.5 Technical Information:Biotechnology GradeDNase(P/F): NoneProtease(P/F): NoneRNase(P/F): NoneAppearance: LiquidBoiling Point : 210-215 °CDensity : 1.33 g/cm3 (Predicted)Melting Point: 234 °C (dec.)PH Value: 7.4RTECS Number: TL6809000HS Code:2933.59.95
Boston Bioproducts Inc HEPES Buffer (1 M, pH 7.5) - 500ml
The HEPES [4-(2-hydroxyethyl)-1-piperazineethanesulfonic acid)] Buffer is a versatile Good's zwitterionic buffering agent widely used in various applications in biochemistry and molecular biology. It maintains a stable pH in the range of 6.8 to 8.2, making it ideal for various applications at physiological pH, including cell culture, protein purification, isoelectric focusing, enzyme studies, bioprocessing, and live cell imaging.
Boston Bioproducts Inc Tris-HCl Buffer (1 M, pH 8.5) - 500ml
The Tris-HCl Buffer is a widely used bufering reagent in molecular biology and biochemistry, acting to maintain a stable pH, and preserve the integrity of target macromolecules. The buffering capacity of Tris-HCl is effective in the pH range of 7.0 to 9.0, making it ideal for many biological applications like cell culture, molecular biology, DNA/RNA extraction, electrophoresis, enzyme assays and immunohistostaining.
Gold Biotechnology Inc Tris (Tris Base) 500 g
GoldBio Tris has the highest purity available on the market. Short for Tris (hydroxymethyl) aminomethane (THAM), Tris is an organic compound often used in buffer solutions such as TAE or TBE for electrophoresis gels. Tris is highly soluble in water and is useful in the pH range 7.0-9.0. It is used in the preparation of Laemmli buffer, one of the most common SDS-PAGE buffers. In addition, Tris can also be used for many custom running and loading buffers, most often with glycine and SDS. The use of single-junction pH electrodes that contain silver should be avoided when determining the pH of a solution containing Tris buffer.
FUJIFILM Biosciences HEPES Buffer Solution 1M
Buffer to maintain pH between 7.2 and 7.6. Suitable for use in cell cultures outside of a CO2 incubator.