Life Science Buffers
Balanced salt solutions and powders used for molecular, protein, nucleic acid, and cell biology applications including cell culture, electrophoresis, ELISA, and chromatography processes; available in a variety of formulations and grades.
Buffer solutions serve to keep pH (acidity or alkalinity) nearly constant in a variety of chemical and biological applications. For example, a bicarbonate buffer helps maintain the pH of blood, a buffered saline maintains cellular contents at a consistent pH level, and buffers help maintain a narrow pH range for enzymes to function correctly.
Most biological samples used in research are kept at a pH of about 7.4. Common biological buffers used for tissue culture include Dulbeccos phosphate buffered saline (PBS), Tris base, HEPES, MOPS, PIPES, and other formulations developed for specific cell lines or applications. Most buffers used in cell culture are also DNase-, RNase-, and protease-free.
The choice of buffer for a particular biological reaction or site depends on several factors:
- Temperature
- Desired or target pH
- Buffer toxicity (to the system)
- Buffer interactions with other system components
Teknova 1X PBS with 0.05% Tween 20, pH 7.4. 1000mL, Sterile.
Phosphate-buffered saline (PBS) is a salt solution that is commonly used in physiological and biochemical experiments. It can be used to maintain pH neutrality or to buffer against changes in pH in order to provide optimal conditions for cellular growth and function. The buffering range is between pH 6.8 and 7.4.
Research Products International Corp MES, Anhydrous, 25 Grams
- CAS Number: 4432-31-9
- Chemical Formula: C6H13NO4S
- Molecular Weight: 195.24 g/mol
Rockland Immunochemicals 1X SDS-PAGE Destain Solution (Ready-to Use) - MB-022-1000
Destain Solution is a uniquely formulated, ready-to-use reagent specifically designed for in-gel visualization of Coomassie Blue stained total protein in polyacrylamide gels. The proprietary formulation of the solution ensures high detection sensitivity and low backgrounds.
Rockland Immunochemicals 1.0 M Tris HCl pH 8.0 - MB-005
Tris solution is an abbreviation of the organic compound known as tris(hydroxymethyl)aminomethane. Tris is extensively used in biochemistry and molecular biology. In biochemistry, Tris is widely used as a component of buffer solutions, such as in TAE and TBE buffer, especially for solutions of nucleic acids.
![Research Products International Corp ACES [N-(2-Acetamido)-2-aminoethane], 100 Grams](https://assets.fishersci.com/TFS-Assets/CCG/product-images/VN00006095-A22500-100.0.JPG-250.jpg)
Research Products International Corp ACES [N-(2-Acetamido)-2-aminoethane], 100 Grams
Zwitterionic buffer used in biochemistry and molecular biology research.
- pKa (25°C): 6.88
- The working pH range in aqueous solution is 6.1 - 7. 5
- Absorbance @ 280nm (5% water): <0.02
Research Products International Corp TRIS, Amino Buffer, Enzyme Grade, 5 Kilograms
- CAS Number: 77-86-1
- Molecular Formula: C4H11NO3
- Molecular Weight: 121.14 g/mol
Sigma Aldrich Fine Chemicals Biosciences Dulbeccos Modified Eagles1L
DULBECCOS Dulbeccos Modified Eagles1L
Sigma Aldrich Fine Chemicals Biosciences Dulbeccos Modified Eagles1L
DULBECCOS Dulbeccos Modified Eagles1L
Sigma Aldrich Fine Chemicals Biosciences Phosphate buffered saline
PHOSPHATE Phosphate buffered saline
Abcam Salsolinol hydrochloride, Dopamine derivative, 5G
MW 215.68 g/mol, Purity >99%. Dopamine (ab120565) derivative. Putative endogenous opioid system-activating factor. Antagonizes L-DOPA-mediated hyperactivity. Shows central effects in vivo. .
The product is subject to the following: Abcam Restricted Use Statement
Abcam Salsolinol hydrochloride, Dopamine derivative, 500MG
MW 215.68 g/mol, Purity >99%. Dopamine (ab120565) derivative. Putative endogenous opioid system-activating factor. Antagonizes L-DOPA-mediated hyperactivity. Shows central effects in vivo. .
The product is subject to the following: Abcam Restricted Use Statement
Sigma Aldrich Fine Chemicals Biosciences Dulbeccos Phosphate Buffer
Dulbecco Phosphate Buffered Saline (DPBS) is a balanced salt SOLUTIONon.
Sigma Aldrich Fine Chemicals Biosciences BIS-TRIS propane BioXtra 925G
REAGENT BIS-TRIS propane BioXtra 925G