Life Science Buffers
Balanced salt solutions and powders used for molecular, protein, nucleic acid, and cell biology applications including cell culture, electrophoresis, ELISA, and chromatography processes; available in a variety of formulations and grades.
Buffer solutions serve to keep pH (acidity or alkalinity) nearly constant in a variety of chemical and biological applications. For example, a bicarbonate buffer helps maintain the pH of blood, a buffered saline maintains cellular contents at a consistent pH level, and buffers help maintain a narrow pH range for enzymes to function correctly.
Most biological samples used in research are kept at a pH of about 7.4. Common biological buffers used for tissue culture include Dulbeccos phosphate buffered saline (PBS), Tris base, HEPES, MOPS, PIPES, and other formulations developed for specific cell lines or applications. Most buffers used in cell culture are also DNase-, RNase-, and protease-free.
The choice of buffer for a particular biological reaction or site depends on several factors:
- Temperature
- Desired or target pH
- Buffer toxicity (to the system)
- Buffer interactions with other system components
Boston Bioproducts Inc Tris-HCl Buffer (1 M, pH 9.5) - 1L
The Tris-HCl Buffer is a widely used bufering reagent in molecular biology and biochemistry, acting to maintain a stable pH, and preserve the integrity of target macromolecules. The buffering capacity of Tris-HCl is effective in the pH range of 7.0 to 9.0, making it ideal for many biological applications like cell culture, molecular biology, DNA/RNA extraction, electrophoresis, enzyme assays and immunohistostaining.
Sigma Aldrich Fine Chemicals Biosciences Phosphate buffered saline powder, pH 7.4, for preparing 5 L solutions | MFCD00131855 | 10/pk
Phosphate buffered saline powder, pH 7.4, for preparing 5 L solutions | | MFCD00131855 | 10/pk
Boston Bioproducts Inc EDTA (0.5 M, pH 7.4, pH Adjusted with KOH) - 250ml
EDTA [Ethylenediaminetetraacetic acid] solution is commonly used in various molecular biology and protein biochemistry applications that require the chelation of divalent metal cations, such as calcium (Ca2+) and magnesium (Mg2+). EDTA is used extensively as an anticoagulant in blood samples as it chelates the calcium present, preventing coagulation and preserving blood cell morphology. It is also used in cell lysis buffers. This solution is adjusted with KOH.
Teknova 200mM Tris, pH 7.0. 1mL, Sterile. 50 Pack
Tris (tris(hydroxymethyl)aminomethane) is used as a component ofbuffer solutions, especially for solutions ofnucleic acids. The useful buffer range for tris (pH 7–9) is similar to the pH of most living organisms. This, and its low cost, make tris one of the most common buffers in the biology/biochemistry laboratory.
Genscript Corporation Tris-MOPS-SDS Running Buffer Powder
Tris-MOPS-SDS Running Buffer Powder are used for ExpressPLUS and SurePAGE gel transfer. Using proprietary techniques, Tris-MOPS-SDS Running Buffer Powder are made to have long shelf life, high resolution, fast electrophoresis, smaller volume.