Life Science Buffers
Balanced salt solutions and powders used for molecular, protein, nucleic acid, and cell biology applications including cell culture, electrophoresis, ELISA, and chromatography processes; available in a variety of formulations and grades.
Buffer solutions serve to keep pH (acidity or alkalinity) nearly constant in a variety of chemical and biological applications. For example, a bicarbonate buffer helps maintain the pH of blood, a buffered saline maintains cellular contents at a consistent pH level, and buffers help maintain a narrow pH range for enzymes to function correctly.
Most biological samples used in research are kept at a pH of about 7.4. Common biological buffers used for tissue culture include Dulbeccos phosphate buffered saline (PBS), Tris base, HEPES, MOPS, PIPES, and other formulations developed for specific cell lines or applications. Most buffers used in cell culture are also DNase-, RNase-, and protease-free.
The choice of buffer for a particular biological reaction or site depends on several factors:
- Temperature
- Desired or target pH
- Buffer toxicity (to the system)
- Buffer interactions with other system components
Sigma Aldrich Fine Chemicals Biosciences Dulbecco's Phosphate Buffered Saline With MgCl2 and CaCl2, liquid, sterile-filtered, suitable for cell culture | MFCD00131855 | 500ML
Dulbecco's Phosphate Buffered Saline With MgCl2 and CaCl2, liquid, sterile-filtered, suitable for cell culture | MFCD00131855 | 500ML
Boston Bioproducts Inc HEPES Buffer (1 M, pH 7.6, pH Adjusted with KOH) - 250ml
The HEPES [4-(2-hydroxyethyl)-1-piperazineethanesulfonic acid)] Buffer is a versatile Good's zwitterionic buffering agent widely used in various applications in biochemistry and molecular biology. It maintains a stable pH in the range of 6.8 to 8.2, making it ideal for various applications at physiological pH, including cell culture, protein purification, isoelectric focusing, enzyme studies, bioprocessing, and live cell imaging. This buffer is adjusted with KOH.
Vector Laboratories Casein Solution 10X 250 ml
Casein Solution is a general blocking agent for immunohistochemistry assays, nucleic acid and protein blotting, and other applications. In this product, it is supplied as a 10x concentrate.
General Laboratory Products Phosphate Buffered Water, 9mL 100/pk
Phosphate Buffered Water - 9ml, 16x125mm polycarbonate tube, 100 per case. For use as a diluent in microbial limit testing of food and water.
Discovery Life Sciences .5M POTASSSIUM PHOS BUFFER 7.4
Potassium Phosphate Buffer, P450 Enzyme Buffer, 0.5M
Boston Bioproducts Inc Tris-Borate-EDTA Buffer (10X) - 4L
The Tris-Borate-EDTA (TBE) Buffer is a pH regulator and chelator used in molecular biology techniques such as nucleic acid gel electrophoresis. Supporting a pH range between 8.1 and 8.5, the TBE Buffer combines aqueous tris base, boric acid, and EDTA (Ethylenediaminetetraacetic Acid), making it efficient as a running buffer or component of agarose gels. This product is prepared in a 10X stock concentration, and can be diluted to concentrations ideal to specific laboratory workflows.
Boston Bioproducts Inc Phosphate Buffered Saline, PBS (pH 7.4, Sterile-Filtered) - 500ml
Phosphate Buffered Saline (PBS) is a versatile buffer solution widely used in biological research due to its isotonic and non-toxic nature to cells. With a pH range of 7.0 to 7.4, PBS mimics the osmolarity and ion concentrations of the human body, making it ideal for a variety of applications including protein purification, as wash buffer in Western blot and immunoassays, sample dilution, and as a transport medium for tissue samples as well as RNA viruses.
Boston Bioproducts Inc MES Buffer (0.5 M, pH 6.5) - 250ml
MES Buffer [2- (N-morpholino) ethanesulfonic acid] is a zwitterionic Good's buffer used in biological and biochemical research. It maintains a stable pH within the range of 5.5 to 7.0 and is highly soluble in water. MES is commonly used as a running buffer for bis-tris gels, chromatography, and in protein purification. It is valuable for enzymatic reactions, and acts as a non-coordinating buffer in metal ion chemistry. It has minimal UV absorbance and is stable in various applications.
Sigma Aldrich Fine Chemicals Biosciences Phosphate Buffered Saline | 1 L
Phosphate buffered saline | Purity: 100% | Mol Wt: N/A | CAS No: N/A | MFCD00011392 | 1LPhosphate buffered saline is an isotonic balanced salt solution used for washing cells and tissues while maintaining physiological pH and osmolarity. It contains sodium chloride, sodium phosphate dibasic, potassium chloride and potassium phosphate monobasic formulated to mimic salt composition, pH and osmolarity of the human body. Lack of calcium and magnesium prevents clotting with blood cells. PBS maintains cell viability and is useful for diluting cells for counting and antibodies for immunofluorescence microscopy. PBS is supplied sterile-filtered without preservatives or endotoxins.
Fd Neurotechnologies Inc PHOSPH-BUF .04PARAFORM W/ PA
Phosphate-Buffered 0.04 Paraformaldehyde with Picric Acid
Abcam Native lysis Buffer, 15ML
Abcam’s 1X Native lysis buffer is an efficient means of cell lysis and protein solubilization for both adherent and suspension cultured mammalian cells.
The product is subject to the following: Abcam Restricted Use Statement