Life Science Buffers
Balanced salt solutions and powders used for molecular, protein, nucleic acid, and cell biology applications including cell culture, electrophoresis, ELISA, and chromatography processes; available in a variety of formulations and grades.
Buffer solutions serve to keep pH (acidity or alkalinity) nearly constant in a variety of chemical and biological applications. For example, a bicarbonate buffer helps maintain the pH of blood, a buffered saline maintains cellular contents at a consistent pH level, and buffers help maintain a narrow pH range for enzymes to function correctly.
Most biological samples used in research are kept at a pH of about 7.4. Common biological buffers used for tissue culture include Dulbeccos phosphate buffered saline (PBS), Tris base, HEPES, MOPS, PIPES, and other formulations developed for specific cell lines or applications. Most buffers used in cell culture are also DNase-, RNase-, and protease-free.
The choice of buffer for a particular biological reaction or site depends on several factors:
- Temperature
- Desired or target pH
- Buffer toxicity (to the system)
- Buffer interactions with other system components
Medchemexpress LLC Bicine | 150-25-4 | 98.0% | 10 G
Bicine is a buffer utilized in physiological research. It is a derivative of the simple amino acid glycine that enhances the sensitivity of guanosine enzyme histochemical detection. This product appears as a white to off-white solid.
- Buffer for physiological research
- Derivative of amino acid glycine
- Enhances sensitivity of guanosine enzyme histochemical detection
- White to off-white solid appearance
- Molecular weight: 163.17
- Chemical formula: C6H13NO4
- Shipped at room temperature
- Stable at room temperature for 3 years
- Stable in solvent at -80°C for 2 years or -20°C for 1 year
- For research use only
Sigma Aldrich Fine Chemicals Biosciences CHES >=99.0% (titration) | 103-47-9 | MFCD00003835 | 25G
CHES >=99.0% (titration) | Purity: >=99.0% (titration) | Mol Wt: 207.29 | 103-47-9 | MFCD00003835 | 25G
![Alkali Scientific PIPES Sesquisodium Salt [Piperazine N,N' bis (2 ethanesulfonic acid) sesquisodium salt], 250 G](https://assets.fishersci.com/TFS-Assets/CCG/product-images/5000661552.jpg-250.jpg)
Alkali Scientific PIPES Sesquisodium Salt [Piperazine N,N' bis (2 ethanesulfonic acid) sesquisodium salt], 250 G
PIPES Sesquisodium Salt (Piperazine N,N' bis(2 ethanesulfonic acid) sesquisodium salt) is a high-purity reagent provided in a 250G pack. It is commonly used as a buffering agent in biochemical and laboratory applications due to its stability at a wide range of pH levels. PIPES is primarily used in the preparation of buffers for biological assays and research, as it does not interfere with biological systems. The sesquisodium salt form helps maintain the pH in both acidic and basic environments. This reagent is suitable for cell culture, enzyme assays, and other biochemical studies where precise pH control is essential. It is also utilized in electrophoresis, chromatography, and immunological testing.
Sigma Aldrich Fine Chemicals Biosciences HEPES potassium salt 99.5250G
HEPES potassium salt 99.5250G
![Alkali Scientific MOPS [3 (N morpholino) propanesulfonic acid], 50 G](https://assets.fishersci.com/TFS-Assets/CCG/product-images/5000660032.jpg-250.jpg)
Alkali Scientific MOPS [3 (N morpholino) propanesulfonic acid], 50 G
MOPS (3-(N-morpholino)propane-1-sulfonic acid) is a biochemical reagent widely used in laboratory applications for pH buffering. This 50g package of MOPS is commonly used for preparing small to medium quantities of buffer solutions for molecular biology, protein chemistry, and electrophoresis. The reagent is effective in maintaining a stable pH range of 6.5 to 7.9, which is essential for stabilizing proteins, nucleic acids, and enzymes during biochemical assays. MOPS is an important component in buffer systems for electrophoresis, chromatography, and enzyme studies where consistent pH is necessary to ensure reliable experimental outcomes.
![Alkali Scientific MES Monohydrate [2 (N Morpholino) ethanesulfonic acid], 500 G](https://assets.fishersci.com/TFS-Assets/CCG/product-images/5000660071.jpg-250.jpg)
Alkali Scientific MES Monohydrate [2 (N Morpholino) ethanesulfonic acid], 500 G
MES Monohydrate (2-(N-Morpholino) ethanesulfonic acid), available in a 500g volume, is a versatile biochemical reagent used for pH buffering in a variety of laboratory and research applications. This buffer is commonly used to stabilize the pH in enzyme assays, cell culture, and biochemical experiments. MES is especially useful in studies where precise pH control is crucial, such as in chromatography, electrophoresis, and protein purification. The 500g volume is ideal for labs requiring bulk quantities of MES for ongoing experiments or high-volume testing. MES ensures minimal interference with biological reactions, making it a reliable choice for many scientific studies in molecular biology and biochemistry.
Boston Bioproducts Inc MES Buffer (1 M, pH 5.0) - 500ml
MES Buffer (1 M pH 5 0) - 500ml (For Research Use and Further Manufacturing Only)
![Research Products International Corp HEPPS [N-(2-Hydroxyethyl)piperazine-N'-(3-propane sulfonic acid)] [EPPS], 25 Grams](https://assets.fishersci.com/TFS-Assets/CCG/product-images/VN00006095-H75060-25.0.JPG-250.jpg)
Research Products International Corp HEPPS [N-(2-Hydroxyethyl)piperazine-N'-(3-propane sulfonic acid)] [EPPS], 25 Grams
- Substance Name: HEPPS [N-(2-Hydroxyethyl)piperazine-N'-(3-propane sulfonic acid)] [EPPS]
- CAS Number: 16052-06-5
- Molecular Formula: C9H20N2O4S
- Molecular Weight: 252.3
Quality Biological Inc HEPES Buffer (1 M), pH 7.3, 100 mL
HEPES is a zwitterionic organic chemical buffering agent. HEPES (1M) is used as a buffer alternative from sodium bicarbonate for cell culture and is widely used in many biochemical reactions and as a buffering agent in some cell culture media. HEPES is reportedly superior to NaHCO3 in controlling pH in tissue and organ culture.
Storage Conditions: 15 °C to 30 °C
Shipping Condition: ambient temperature
Sterile Filtered
Performance Tested
pH: 7.2-7.4
Max Shelf Life: 24 months from date of manufacture
Manufactured under an ISO 9001:2015 certified quality management system.
Product and/or packaging contains recycled/renewable content. Product and/or packaging is recyclable or reduces waste. Product and/or packaging is safer to human and environmental health.
eMolecules 2,4-Dichloro-N-(2-hydroxyethyl)benzenecarboxamide | 20656-08-0 | MFCD00141691 | 1g
Matrix Scientific | 2,4-Dichloro-N-(2-hydroxyethyl)benzenecarboxamide | 1g | 389721058 | 45569 | 95.000 | 20656-08-0 | MFCD00141691 | 234.080 | C9H9Cl2NO2
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Chem-Impex International, Inc. 4-(2-Hydroxyethyl)-1-piperazineethanesulfonic acid | 7365-45-9 | MFCD00006158 | 25G
4-(2-Hydroxyethyl)-1-piperazineethanesulfonic acid, 7365-45-9, MFCD00006158, 25G
![Research Products International Corp HEPES, Sodium Salt [N-(2-Hydroxyethyl) piperazine-N'-(2-ethanesulfonic acid)], 1 Kilogram](https://assets.fishersci.com/TFS-Assets/CCG/product-images/VN00006095-H75050-1000.0.JPG-250.jpg)
Research Products International Corp HEPES, Sodium Salt [N-(2-Hydroxyethyl) piperazine-N'-(2-ethanesulfonic acid)], 1 Kilogram
pKa (20°C): 7.45-7.65
pH Range: 6.8-8.2
Sigma Aldrich Fine Chemicals Biosciences POPSO hydrate >=99% (titration) | 68189-43-5 (anhydrous) | 100G
POPSO hydrate >=99% (titration) | Purity: >=99% (titration) | 68189-43-5 (anhydrous) | 100G