Life Science Buffers
Balanced salt solutions and powders used for molecular, protein, nucleic acid, and cell biology applications including cell culture, electrophoresis, ELISA, and chromatography processes; available in a variety of formulations and grades.
Buffer solutions serve to keep pH (acidity or alkalinity) nearly constant in a variety of chemical and biological applications. For example, a bicarbonate buffer helps maintain the pH of blood, a buffered saline maintains cellular contents at a consistent pH level, and buffers help maintain a narrow pH range for enzymes to function correctly.
Most biological samples used in research are kept at a pH of about 7.4. Common biological buffers used for tissue culture include Dulbeccos phosphate buffered saline (PBS), Tris base, HEPES, MOPS, PIPES, and other formulations developed for specific cell lines or applications. Most buffers used in cell culture are also DNase-, RNase-, and protease-free.
The choice of buffer for a particular biological reaction or site depends on several factors:
- Temperature
- Desired or target pH
- Buffer toxicity (to the system)
- Buffer interactions with other system components
Research Products International Corp Bicine Ultra-Pure, 500 Grams
Zwitterionic buffer used in a variety of molecular biology applications such as protein resolution, protein crystallization and SDS-PAGE.
pKa @ 25°C: 8.3
pH Range: 7.6-9.0
Melting Range: 190-192°C
Research Products International Corp TRIS-BORATE-EDTA, 10X SOLUTION, 4 LITER
TBE is a common buffer used in gel electrophoresis.
Supplied as a 1X concentrated solution containing:
89mM Boric Acid
2mM EDTA disodium salt
pH (1X solution @ 25°:C): 8.3+0.1
![Research Products International Corp TES, Free Acid [2-Tris(hydroxymethyl)-methyl-2-amino 1-ethanesulfonic acid], 100 Grams](https://assets.fishersci.com/TFS-Assets/CCG/product-images/VN00006095-T31022-100.0.JPG-250.jpg)
Research Products International Corp TES, Free Acid [2-Tris(hydroxymethyl)-methyl-2-amino 1-ethanesulfonic acid], 100 Grams
Substance Name: 2-Tris(hydroxymethyl)-methyl-2-amino 1-ethanesulfonic acid
CAS Number: 7365-44-8
Molecular Formula: C6H15NO6S
Molecular Weight: 229.25 g/mol
STEMCELL Technologies HEPES Buffer Solution (1 M), Size: 100 mL
HEPES Buffer Solution is a biological buffer used in cell culture media. Selection of suitable nutrient medium is dependent on type of cell, conditions of culture, and degree of chemical definition required for the cell culture application.
Vmrd Inc 4X FA Powdered Rinse Buffer
FA Rinse Buffer, pH 9.0. Powder to make one liter of 4x rinse buffer. Used to rinse fluorescent antibody antibody substrate slides.
Sigma Aldrich Fine Chemicals Biosciences Phosphate Buffered Saline | MFCD00131855 | 1L
Phosphate Buffered Saline | MFCD00131855 | 1L