Life Science Buffers
Balanced salt solutions and powders used for molecular, protein, nucleic acid, and cell biology applications including cell culture, electrophoresis, ELISA, and chromatography processes; available in a variety of formulations and grades.
Buffer solutions serve to keep pH (acidity or alkalinity) nearly constant in a variety of chemical and biological applications. For example, a bicarbonate buffer helps maintain the pH of blood, a buffered saline maintains cellular contents at a consistent pH level, and buffers help maintain a narrow pH range for enzymes to function correctly.
Most biological samples used in research are kept at a pH of about 7.4. Common biological buffers used for tissue culture include Dulbeccos phosphate buffered saline (PBS), Tris base, HEPES, MOPS, PIPES, and other formulations developed for specific cell lines or applications. Most buffers used in cell culture are also DNase-, RNase-, and protease-free.
The choice of buffer for a particular biological reaction or site depends on several factors:
- Temperature
- Desired or target pH
- Buffer toxicity (to the system)
- Buffer interactions with other system components
Tris Hydrochloride, 1M Solution (pH 7.5/Mol. Biol.), Fisher BioReagents™
CAS: 77-86-1 Molecular Formula: C4H11NO3 Molecular Weight (g/mol): 121.136 InChI Key: LENZDBCJOHFCAS-UHFFFAOYSA-N Synonym: Tris-HCl PubChem CID: 6503 ChEBI: CHEBI:9754 IUPAC Name: 2-amino-2-(hydroxymethyl)propane-1,3-diol SMILES: C(C(CO)(CO)N)O
HEPES Sodium Salt (White Powder), Fisher BioReagents
CAS: 75277-39-3 Molecular Formula: C8H17N2NaO4S Molecular Weight (g/mol): 260.28 MDL Number: MFCD00036463 InChI Key: RDZTWEVXRGYCFV-UHFFFAOYSA-M PubChem CID: 2724248 ChEBI: CHEBI:46758 IUPAC Name: sodium 2-[4-(2-hydroxyethyl)piperazin-1-yl]ethane-1-sulfonate SMILES: [Na+].OCCN1CCN(CCS([O-])(=O)=O)CC1
Bis-Tris, Fisher BioReagents
CAS: 6976-37-0 Molecular Formula: C8H19NO5 Molecular Weight (g/mol): 209.242 InChI Key: OWMVSZAMULFTJU-UHFFFAOYSA-N Synonym: 2,2-Bis(hydroxymethyl)-2,2?,2?-nitrilotriethanol,2, 2-Bis(hydroxymethyl)-2, 2?,Bis(2-hydroxyethyl)amino-tris(hydroxymethyl)methane PubChem CID: 81462 ChEBI: CHEBI:41250 IUPAC Name: 2-[bis(2-hydroxyethyl)amino]-2-(hydroxymethyl)propane-1,3-diol SMILES: C(CO)N(CCO)C(CO)(CO)CO
Thermo Scientific Chemicals HEPES, 1.0M buffer soln., pH 8.0
CAS: 7365-45-9 Molecular Formula: C8H18N2O4S Molecular Weight (g/mol): 238.30 MDL Number: MFCD00006158 InChI Key: JKMHFZQWWAIEOD-UHFFFAOYSA-N PubChem CID: 23831 ChEBI: CHEBI:42334 IUPAC Name: 2-[4-(2-hydroxyethyl)piperazin-1-yl]ethanesulfonic acid SMILES: OCCN1CCN(CCS(O)(=O)=O)CC1
Invitrogen™ NuPAGE™ MES SDS Running Buffer (Powder)
Invitrogen NuPAGE MES SDS Running Buffer (Powder) is formulated for use with Bis-Tris gels to deliver fast SDS-PAGE separations of small- to medium-sized proteins. Supplied as a powder for flexible preparation, room temperature storage, and a long 2-year shelf life.
Thermo Scientific Chemicals TRIS-buffered saline (TBS, 10X) pH 7.4, for Western blot
CAS: 77-86-1 Molecular Formula: C4H11NO3 Molecular Weight (g/mol): 121.136 MDL Number: MFCD00004679 InChI Key: LENZDBCJOHFCAS-UHFFFAOYSA-N PubChem CID: 6503 ChEBI: CHEBI:9754 IUPAC Name: 2-amino-2-(hydroxymethyl)propane-1,3-diol SMILES: C(C(CO)(CO)N)O
Invitrogen™ 10X TBE (powder)
Invitrogen 10X TBE (Tris-Borate-EDTA Buffer) is thoroughly tested for RNase and DNase contamination, making it ideal for use in sensitive application.
Invitrogen™ NuPAGE™ MOPS SDS Running Buffer (Powder)
Invitrogen NuPAGE MOPS SDS Running Buffer (Powder) is formulated for use with Bis-Tris gels to resolve medium- to large-sized proteins by providing slower SDS-PAGE separations. Supplied as a powder for flexible preparation, room temperature storage, and a long 2-year shelf life.
Invitrogen™ TBE (10X), RNase-free
Molecular biology grade, Invitrogen 10X TBE solution is supplied in four bottles containing 1 L each. The buffer is certified RNase-free, economical, and ready-to-use.
Invitrogen™ Novex™ Rapid Tris-Glycine SDS Running Buffer (Powder)
Invitrogen Novex Rapid Tris-Glycine SDS Running Buffer (Powder) delivers more than 50% faster SDS-PAGE run times compared to traditional Tris-Glycine buffers, without compromising separation performance. Compatible with all Tris-Glycine gels, it offers room temperature storage and a 2-year shelf life.
Gibco™ HEPES (1 M)
Gibco HEPES (4-(2-hydroxyethyl)-1-piperazineethanesulfonic acid) is a zwitterionic organic chemical buffering agent commonly used in cell culture media. It is membrane impermeable, relatively stable, and has limited interference in key processes.
Gibco™ HBSS, calcium, magnesium
Hanks' Balanced Salt Solution (HBSS) is used for a variety of cell culture applications, such as washing cells before dissociation, transporting cells or tissue, diluting cells for counting, and preparing reagents.
Gibco™ PBS Tablets
Phosphate-buffered saline (PBS) is a balanced salt formulation used for a variety of cell culture applications, such as washing cells before dissociation, transporting cells or tissue, diluting cells for counting, and preparing reagents.
Invitrogen™ xMAP Sheath Fluid Plus
xMAP Sheath Fluid Plus is the delivery medium that carries the sample to the optics component of Luminex xMAP technology-based instruments.
Gibco™ HBSS (10X), calcium, magnesium, phenol red
Hanks' Balanced Salt Solution (HBSS) is used for a variety of cell culture applications, such as washing cells before dissociation, transporting cells or tissue, diluting cells for counting, and preparing reagents.