Life Science Buffers
Balanced salt solutions and powders used for molecular, protein, nucleic acid, and cell biology applications including cell culture, electrophoresis, ELISA, and chromatography processes; available in a variety of formulations and grades.
Buffer solutions serve to keep pH (acidity or alkalinity) nearly constant in a variety of chemical and biological applications. For example, a bicarbonate buffer helps maintain the pH of blood, a buffered saline maintains cellular contents at a consistent pH level, and buffers help maintain a narrow pH range for enzymes to function correctly.
Most biological samples used in research are kept at a pH of about 7.4. Common biological buffers used for tissue culture include Dulbeccos phosphate buffered saline (PBS), Tris base, HEPES, MOPS, PIPES, and other formulations developed for specific cell lines or applications. Most buffers used in cell culture are also DNase-, RNase-, and protease-free.
The choice of buffer for a particular biological reaction or site depends on several factors:
- Temperature
- Desired or target pH
- Buffer toxicity (to the system)
- Buffer interactions with other system components
Zymo Research Corporation DNA Wash Buffer (Concentrate), 24 ml
This ensures high purity DNA to be recovered. Ethanol must be added to the concentrate prior to use. Instructions for each DNA Wash Buffer (concentrate) size is listed on the bottle and within the Zymo Research protocols it is used in.
Boston Bioproducts Inc Molecular Biology Grade Water - 1L
Molecular Biology Grade Water - 1L (For Research Use and Further Manufacturing Only)
Zymo Research Corporation DNA Elution Buffer, 4 ml
This can be used with any of the DNA purification for DNA elution from columns, plates, and magbeads.
Sigma Aldrich Fine Chemicals Biosciences Ethylenediaminetetraacetic acid BioUltra, >=99.0% (KT) | 60-00-4 | MFCD00003541 | 1KG
Ethylenediaminetetraacetic acid BioUltra, >=99.0% (KT) | Purity: >=99.0% (KT) | Mol Wt: 292.24 | 60-00-4 | MFCD00003541 | 1KG
Alkali Scientific CellPro™ Tris Glycine Buffer 10X, 1L x 12/cs
Alkali Scientific™ CellPro™ Tris Glycine offers a sterile solution ideal for a variety of biochemical and molecular biology applications. Composed of Tris (Tris(hydroxymethyl)aminomethane) and Glycine, this buffer provides optimal conditions for protein electrophoresis, particularly in SDS-PAGE (Sodium Dodecyl Sulfate Polyacrylamide Gel Electrophoresis) systems. The sterile formulation ensures the purity and integrity of your samples, minimizing the risk of contamination and ensuring reliable results. CellPro™ Tris Glycine buffer is versatile and suitable for use in protein separation, transfer, and analysis techniques, making it an essential component in the laboratory for protein research and characterization. Trust in the quality and consistency of CellPro™ Tris Glycine for your protein electrophoresis experiments.
Boston Bioproducts Inc Molecular Biology Grade Water - 500ml
Molecular Biology Grade Water - 500ml (For Research Use and Further Manufacturing Only)
Bioworld PEB- Protein Extraction Buffer (5x), 250 mL
Tris Protein Extraction Buffer (PEB), 5X Lysis buffer for extracting proteins from cells. Technical Information:5X concentrationTris-HCL, pH 8.0 (0.5M)B-mercaptoethanol (100mM)EDTA (20mM)Add protease inhibitor according to your protocol
Electron Microscopy Sciences Tris Maleate Buffer 0.2M, pH range 6.4-8.0 250 ML
Please specify pH when ordering.
pH: 6.4-8.0
Boston Bioproducts Inc Tris-HCl Buffer (1 M, pH 6.5) - 500ml
The Tris-HCl Buffer is a widely used bufering reagent in molecular biology and biochemistry, acting to maintain a stable pH, and preserve the integrity of target macromolecules. The buffering capacity of Tris-HCl is effective in the pH range of 7.0 to 9.0, making it ideal for many biological applications like cell culture, molecular biology, DNA/RNA extraction, electrophoresis, enzyme assays and immunohistostaining.
Hampton Research HR2-900-11/StockOptions Tris #11 pH 8.0, 185 ml
HR2-900-11/StockOptions Tris #11 pH 8.0, 185 ml. Please note items from this supplier are non-returnable.