Life Science Buffers
Balanced salt solutions and powders used for molecular, protein, nucleic acid, and cell biology applications including cell culture, electrophoresis, ELISA, and chromatography processes; available in a variety of formulations and grades.
Buffer solutions serve to keep pH (acidity or alkalinity) nearly constant in a variety of chemical and biological applications. For example, a bicarbonate buffer helps maintain the pH of blood, a buffered saline maintains cellular contents at a consistent pH level, and buffers help maintain a narrow pH range for enzymes to function correctly.
Most biological samples used in research are kept at a pH of about 7.4. Common biological buffers used for tissue culture include Dulbeccos phosphate buffered saline (PBS), Tris base, HEPES, MOPS, PIPES, and other formulations developed for specific cell lines or applications. Most buffers used in cell culture are also DNase-, RNase-, and protease-free.
The choice of buffer for a particular biological reaction or site depends on several factors:
- Temperature
- Desired or target pH
- Buffer toxicity (to the system)
- Buffer interactions with other system components
Andwin Scientific HEPES ACID FREE 100 gram
Hepes is a solution made to perform as a zwitterionic N-substituted aminosulfonic acid buffer. It is ideal for use in the pH 6.8-8.2 range. This product has a pKa of 7.48 at 25C. This product has a Health Hazard NFPA Rating of 2, a Fire Hazard NFPA Rating of 1, and a Reactivity NFPA Rating of 0. It is packaged and sold in a 100 gram glass bottle.
SIGMA ALDRICH FINE CHEMICALS BIOSCIENCES HEPES BIOPERFORMANCE 99.5
NC3239327 HEPES BIOPERFORMANCE 99.5
Kd Medical Inc TRIS HCL, 1M PH 6.8, 500ML
Tris, Hydrochloride is commonly used as a buffer component for phenol extractions of DNA or RNA and a buffering component of separating and stacking gels in the characterization of protein products by SDS-PAGE. 1M solution, pH 6.8. One 500ml bottle.
Kd Medical Inc PBS BUFFER, 1X, PH 7.2, 500ML
1X PBS working solution commonly used in biological research. Used as a wash buffer in several research applications. One 500ml bottle.
Kd Medical Inc TBE (TRIS-BORATE EDTA), 10X
Tris Borate EDTA is used for polyacrylamide and agarose gel electrophoresis. 10X TBE is concentrated and ready to be diluted to a 1X working solution. One 500ml bottle.
Kd Medical Inc TRIS-HCL BUFFER, 1M,PH 7.4, 1L
Used for molecular biology, protein chemistry, and biochemical applications. 1 Molar solution, pH 7.4. One 1 liter bottle.
Kd Medical Inc PBS BUFFER, 1X, PH 7.4, 500ML
1X PBS working solution commonly used in biological research. Used as a wash buffer in several research applications. One 500ml bottle.
Kd Medical Inc TRIS BUFFER, 2M, PH 8.0, 1L
Buffer used in a wide range of Molecular Biology applications. 2 Molar solution pH 8.0. One 1 Liter bottle.
Kd Medical Inc STERILE SALINE 0.9%, 100ML BTL
Solution used for sterile irrigation, rinsing, dilution and cell washing purposes. One 100ml bottle.
Kd Medical Inc TRIS-HCL BUFFER 1M, PH 7.5, 1L
Used for molecular biology, protein chemistry, and biochemical applications. 1 Molar solution, pH 7.5. One 1 liter bottle.
Growcells TAE BUFFER 50X 1L EA
Sterile liquid electrophoresis buffer concentrate prepared with 18.2 megOhm water, 0.2 micron sterile filtered and steam sterilized in final packaging. Recipe/L: 2424.8g TRIS Base, 570ml Acetic Acid, 186.1g EDTA Ph 8.3
Growcells PBS 20X 2L EA
Sterile 20X Phosphate-Buffered Saline solution concentrate prepared with 18.2 megOhm water, 0.2 micron sterile filtered and steam sterilized in final packaging. Recipe/L: NaCl 160g, KCl 4g, Na2HPO4 28.8g, KH2PO4 4.8g
Growcells TRIS-EDTA PH8 1L EA
Ready to use, sterile TE (Tris-EDTA) buffer solution prepared with 18.2 megOhm water, 0.2 micron sterile filtered and steam sterilized in final packaging. pH 8.0