Life Science Buffers
Balanced salt solutions and powders used for molecular, protein, nucleic acid, and cell biology applications including cell culture, electrophoresis, ELISA, and chromatography processes; available in a variety of formulations and grades.
Buffer solutions serve to keep pH (acidity or alkalinity) nearly constant in a variety of chemical and biological applications. For example, a bicarbonate buffer helps maintain the pH of blood, a buffered saline maintains cellular contents at a consistent pH level, and buffers help maintain a narrow pH range for enzymes to function correctly.
Most biological samples used in research are kept at a pH of about 7.4. Common biological buffers used for tissue culture include Dulbeccos phosphate buffered saline (PBS), Tris base, HEPES, MOPS, PIPES, and other formulations developed for specific cell lines or applications. Most buffers used in cell culture are also DNase-, RNase-, and protease-free.
The choice of buffer for a particular biological reaction or site depends on several factors:
- Temperature
- Desired or target pH
- Buffer toxicity (to the system)
- Buffer interactions with other system components
Quality Biological Inc DPBS (10X) without Calcium and Magnesium, 500 mL
DPBS is a balanced salt solution used for the handling and culturing of mammalian cells. DPBS without calcium and magnesium can be used to wash and rinse suspended cells. irrigating, transporting or diluting fluid for mammalian cells, while maintaining cell tonicity and viability for a limited period of time.
Without Calcium and Magnesium
Without Phenol Red
Storage Condition: 15 °C to 30 °C
Shipping Condition: ambient temperature
Sterile Filtered
Endotoxin and Mycoplasma Tested
Max Shelf Life: 24 months from date of manufacture
Manufactured under an ISO 9001:2015 certified quality management system.
Product and/or packaging contains recycled/renewable content. Product and/or packaging is recyclable or reduces waste. Product and/or packaging is safer to human and environmental health.
Alkali Scientific CellPro™ Phosphate Buffered Saline 10X, with Ca and Mg, pH 7.2-7.4, 500mL
Alkali Scientific™ CellPro™ PBS (Phosphate Buffered Saline) provides researchers with a reliable and consistent solution essential for various molecular and cellular biology applications. This meticulously prepared buffer is formulated to maintain a stable pH and osmolarity, ensuring optimal conditions for cell culture, protein assays, immunohistochemistry, and many other biological techniques. With its phosphate-based formulation, CellPro™ PBS offers excellent buffering capacity, making it suitable for maintaining the physiological pH of biological samples and preserving their integrity. Whether used for cell washing, sample dilution, or buffer exchange, CellPro™ PBS guarantees exceptional performance and reproducibility in every experiment. Trust in the quality and reliability of CellPro™ PBS to support your research needs and enable precise and consistent results.
Cell Signaling Technology Phosphate Buffered Saline (PBS-1X) pH7.2 (Sterile) 10 ml
Phosphate Buffered Saline (PBS-1X) pH7.2 (Sterile) 10 ml
AG Scientific Inc Phosphate Buffered Saline 1 L
Phosphate Buffered Saline is a buffer solution commonly used in biological research. PBS has many uses because it is isotonic and non-toxic to most cells. These uses include substance dilution and cell container rinsing.Ready to use sterile 1X solution, pH: 7.25 - 7.55
Enzo Life Sciences PBS (endotoxin-free) (sterile) (1.5ml)
Sterile and endotoxin-free PBS for use with TLRGRADE™ or endotoxin-free grade reagents. Product has been subjected to multiple rounds of LPS removal by adsorption with activated charcoal. Long Term Storage: +4°C.
Sigma Aldrich Fine Chemicals Biosciences Dulbeccos Modified Eagles500ML
Pyridoxal which is an aldehyde has been replaced with pyridoxine. This modification has been shown to improve the stability of the medium. Suitable for growth of a wide range of cell types in low serum conditions. Absence of phenol red makes this a preferred medium for clonal and stem cell culture.
Biotang Inc RNAse Free Phosphate-Buffered Saline, 250 ml
RNAse Free Phosphate-Buffered Saline, 250 ml.
IBI SCIENTIFIC 3P 1X PBST (PBS W/0.05% TWEEN 20) 6/PACK
" Sterile buffer solution containing sodium phosphate, sodium chloride, potassium phosphate and potassium chloride with 0.05% TWEEN 20.Sterile filtered using 0.1um filterDNase, RNase and protease free.Sterility validated using USP <71> Test for SterilityDoes not contain calcium, magnesium, phenol red or sodium pyruvate. IBI 1X PBST (1X PBS) with TWEEN 20 (0.05% TWEEN 20) is a ready-to-use sterile solution in a 1 liter bottle that is ideal for use in immunoassay procedures such as Western Blotting and ELISAs in microtiter plate wells.IBI 1X PBST is mixed and bottled in our liquid production facility here in Dubuque, Iowa. Our high output pure water system produces endotoxin free and nuclease free water at a rate of 100 gallons per/hr. This water is the basis for all of our liquid blending production here at IBI."
Alkali Scientific Protease Inhibitor Cocktail, EDTA Free, 20 Tablets
Protease Inhibitor Cocktail, EDTA-Free (Tablet Form), is a versatile enzyme inhibitor formulation that effectively prevents protease activity during protein extraction. Offered in a convenient tablet form, this product allows for easy and precise inhibitor preparation in a range of biological samples. The EDTA-free formulation is particularly useful when metal ion-dependent proteases need to be targeted without interfering with sample components. This product is widely used for protein isolation from tissues and cells, particularly in applications like immunoassays, Western blotting, and enzyme activity studies, where protein integrity is critical.
Fluidigm Corp Maxpar PBS, 500 mL
Formulated to provide the best possible signal to noise ratio for detection by mass cytometry.