Life Science Buffers
Balanced salt solutions and powders used for molecular, protein, nucleic acid, and cell biology applications including cell culture, electrophoresis, ELISA, and chromatography processes; available in a variety of formulations and grades.
Buffer solutions serve to keep pH (acidity or alkalinity) nearly constant in a variety of chemical and biological applications. For example, a bicarbonate buffer helps maintain the pH of blood, a buffered saline maintains cellular contents at a consistent pH level, and buffers help maintain a narrow pH range for enzymes to function correctly.
Most biological samples used in research are kept at a pH of about 7.4. Common biological buffers used for tissue culture include Dulbeccos phosphate buffered saline (PBS), Tris base, HEPES, MOPS, PIPES, and other formulations developed for specific cell lines or applications. Most buffers used in cell culture are also DNase-, RNase-, and protease-free.
The choice of buffer for a particular biological reaction or site depends on several factors:
- Temperature
- Desired or target pH
- Buffer toxicity (to the system)
- Buffer interactions with other system components
Teknova 1.5M Tris-HCl, pH 8.8. 1000mL, Sterile.
Tris (tris(hydroxymethyl)aminomethane) is used as a component ofbuffer solutions, especially for solutions ofnucleic acids. The useful buffer range for tris (pH 7–9) is similar to the pH of most living organisms. This, and its low cost, make tris one of the most common buffers in the biology/biochemistry laboratory.
Quality Biological Inc TBS (10X) pH 7.4, Case 10 X 1000 mL
TBS (10X), pH 7.4 is used as a washing buffer for cellular and molecular biology techniques.
DNase, RNase and Protease tested.
Applications: TBS (10X), pH 7.4 will be useful as a washing buffer for electrodes, microtiter plates, and alkaline phophatase or peroxidase conjugates. TBS (10X), pH 7.44 can also be used in detergent preparation and Western blot or ELISA applications.
Storage Conditions: 15 °C to 30 °C
Shipping Condition: ambient temperature
Sterile Filtered
DNase, RNase, and Protease Tested
pH: 7.4 ± 0.1
Max Shelf Life: 36 months from date of manufacture
Product and/or packaging contains recycled/renewable content. Product and/or packaging is recyclable or reduces waste. Product and/or packaging is safer to human and environmental health.
Hampton Research HR2-579/1.0 M TRIS hydrochloride - 100 ml
HR2-579/1.0 M TRIS hydrochloride - 100 ml. Please note items from this supplier are non-returnable.
Bioworld STEBuffer (1X), 4 L
STE Reagent Common reagent for molecular biology. Used in DNA extraction, DNA electrophoresis and many other lab protocols.DNAse and RNAse free, ready to use 1X solution Technical Information:NaCl (100mM) Tris-HCl, pH 8.0 (10mM) EDTA, pH 8.0 (1mM)
Teknova 1M Ammonium Sulfate, 10mM Tris-HCl pH 8.0. 500mL, Sterile. 4 Pack.
1M Ammonium Sulfate,10mM Tris-HCl
Bioworld TE Buffer (10X), 100 mL
Product DescriptionTE (Tris-EDTA) has a liquid concentrate of 10X with a pH level of 8.0 brought up with HCl and is stored at room temperature. It contains 10 mM Tris and 1 mM EDTA. It is of biotechnology grade and no DNAse or RNAse activity is detected. TE buffer is used to solubilize DNA or RNA while protecting it from degradation. Commonly used for DNA isolation and electrophoresis protocols.
Sigma Aldrich Fine Chemicals Biosciences Piperazine BioUltra, anhydrous, >=99.0% (T) | 110-85-0 | MFCD00005953 | 25G
Piperazine BioUltra, anhydrous, >=99.0% (T) | Purity: >=99.0% (T) | Mol Wt: 86.14 | 110-85-0 | MFCD00005953 | 25G
Bioworld Tris-Glycine Transfer Buffer (10X), 1 L
Tris-Glycine Transfer Buffer, 10X Concentrate.For Western blotting and gel electrophoresis. Tris-glycine buffer is used to make a Tris-glycine-methanol transfer buffer, which is the most common protein transfer buffer for wet blot transfers. The methanol prevents the gel from swelling during the transfer and enhances the protein binding to nitrocellulose.The 10× Tris-glycine buffer is diluted to 1× with methanol and water to make a solution containing 25 mM Tris, 192 mM glycine, and 20% methanol. A sufficient amount of transfer buffer should be made to cover the electrode wires in the wet blot transfer unit and to soak the gel, membrane and blotting paper.To make 1X Transfer Buffer from 10X concentrate: Mix 100 ml of 10X Transfer Buffer, 200 ml of methanol and 700 ml of dd water per liter.Foreign activity: Protease, none detected Storage temp.: 2-8°C
Abcam Mammalian Cell Lysis Buffer 5X (ab179835)
Mammalian Cell Lysis Buffer 5X (ab179835)
The product is subject to the following: Abcam Restricted Use Statement
Sigma Aldrich Fine Chemicals Biosciences MOPS >=99.5% (titration) | 1132-61-2 | MFCD00006183 | 25G
MOPS >=99.5% (titration) | Purity: >=99.5% (titration) | Mol Wt: 209.26 | 1132-61-2 | MFCD00006183 | 25G