Life Science Buffers
Balanced salt solutions and powders used for molecular, protein, nucleic acid, and cell biology applications including cell culture, electrophoresis, ELISA, and chromatography processes; available in a variety of formulations and grades.
Buffer solutions serve to keep pH (acidity or alkalinity) nearly constant in a variety of chemical and biological applications. For example, a bicarbonate buffer helps maintain the pH of blood, a buffered saline maintains cellular contents at a consistent pH level, and buffers help maintain a narrow pH range for enzymes to function correctly.
Most biological samples used in research are kept at a pH of about 7.4. Common biological buffers used for tissue culture include Dulbeccos phosphate buffered saline (PBS), Tris base, HEPES, MOPS, PIPES, and other formulations developed for specific cell lines or applications. Most buffers used in cell culture are also DNase-, RNase-, and protease-free.
The choice of buffer for a particular biological reaction or site depends on several factors:
- Temperature
- Desired or target pH
- Buffer toxicity (to the system)
- Buffer interactions with other system components
Teknova 1M MES, pH 5.76. 1000mL, Sterile 2 pack.
MES solution is a zwitterionic buffer used to help maintain pH levels in biochemistry experiments. It has a buffering range of pH 5.8 - 6.5.
Teknova 50X TAE, Electrophoresis Grade. (2M Tris-Acetate, 100mM EDTA) 1000mL, Non-Sterile. 2 pack.
2M Tris-Acetate, 100mM EDTA
Gold Biotechnology Inc HEPPSO 100 g
HEPPSO is a buffering agent used in biochemistry and molecular biology that was selected and described by Good et al. It is a zwitterionic, piperazinic buffer that is useful for a pH range of 7.1 – 8.5. It is commonly used as an ampholytic separator to create a pH gradient in isoelectric focusing. HEPPSO displays no toxicity for crustaceans with which it has been studied and has been deemed suitable for toxicological studies. This buffer is capable of forming radicals and is therefore not suitable for redox reactions. HEPPSO forms a complex with Cu(II) ions so stability constants and concentrations should be taken into consideration when using this buffer in a solution containing copper ions. Though HEPPSO binds to coppers ions, it is still suitable for use with the bicinchoninic acid (BCA) assay. Diminished protein-induced color development can be accounted for or even eliminated by precipitating protein prior to reaction with bicinchoninic acid.
Bioworld ACE MES Buffer, 50mM, pH 6.0, 250 mL
Product DescriptionA.C.E.™ MES (compound 2-(N-morpholino)ethanesulfonic acid) buffer has a concentration of 50 mM with a pH level of 6.0. The buffer is biotechnology grade and is formulated for capillary electrophoresis. Buffer is made with 3D water and filtered through 0.22 µ filter. Product is shipped at room temperature.
Bioworld ACE MES Buffer, 50mM, pH 6.0, 100 mL
Product DescriptionA.C.E.™ MES (compound 2-(N-morpholino)ethanesulfonic acid) buffer has a concentration of 50 mM with a pH level of 6.0. The buffer is biotechnology grade and is formulated for capillary electrophoresis. Buffer is made with 3D water and filtered through 0.22 µ filter. Product is shipped at room temperature.
Bioworld Tris-Glycine Transfer Buffer (10X), 1 L
Tris-Glycine Transfer Buffer, 10X Concentrate.For Western blotting and gel electrophoresis. Tris-glycine buffer is used to make a Tris-glycine-methanol transfer buffer, which is the most common protein transfer buffer for wet blot transfers. The methanol prevents the gel from swelling during the transfer and enhances the protein binding to nitrocellulose.The 10× Tris-glycine buffer is diluted to 1× with methanol and water to make a solution containing 25 mM Tris, 192 mM glycine, and 20% methanol. A sufficient amount of transfer buffer should be made to cover the electrode wires in the wet blot transfer unit and to soak the gel, membrane and blotting paper.To make 1X Transfer Buffer from 10X concentrate: Mix 100 ml of 10X Transfer Buffer, 200 ml of methanol and 700 ml of dd water per liter.Foreign activity: Protease, none detected Storage temp.: 2-8°C
Bioworld Tris-Glycine Sample Buffer (4X), 25 mL
Product DescriptionTris-Glycine (TG) sample buffer has a concentration of 4X. It contains 400 mM Tris-HCl at a pH of 8.6, 40% glycerol, and 0.02% bromophenol blue.TG buffers are blended using pure reagents to provide high quality for protein research. This buffer is formulated to ensure exceptional purity along with the absence of nuclease and protease activities. TG buffer is widely used for electroblotting proteins in Western blot procedures and is recommended for use with all nitrocellulose and nylon membranes.
National Diagnostics SEQUAGEL BUFFER 200 ML
SequaGel Buffer, System consists of SequaGel Concentrate, SequaGel Diluent, and SequaGel Buffer for casting 19:1 denaturing DNA gels of a varying percentage, 200 ML
National Diagnostics ProtoGel Resolving Buffer (4X) 450ML
Traditional Laemmli Resolving Gel Buffer (4X). Formulated with 0.2 Micron Filtration.Ultra-Pure Reagents in 18 MegOhm Water.Crystal Clear, Reproducible Gels. Catalog number: EC-892. The use of National Diagnostics' ProtoGel Resolving Buffer will ensure the purity and performance of your Laemmli gels. When diluted as directed ProtoGel Resolving Buffer forms a gel of 0.375 M Tris-HCl and 0.1% SDS, pH 8.8. Storage: ProtoGel Resolving Buffer is stable for 24 months when stored tightly capped in a dark area at room temperature.
National Diagnostics PROTOGEL STACKING BUFFER 200 M
ProtoGel Stacking Buffer, 4X Laemmli Tris-HCl stacking gel buffer for clear, reproducible SDS-PAGE, 200 ML
National Diagnostics TRIS-GLYCINE ELECTROBLOTTING B
Tris-Glycine Electroblotting Buffer (10X), Stable, economical, ultra pure transfer buffer for Western Blotting procedures. 18 megOhm water. 0.2 micron filtration, 1 LT