Life Science Buffers
Balanced salt solutions and powders used for molecular, protein, nucleic acid, and cell biology applications including cell culture, electrophoresis, ELISA, and chromatography processes; available in a variety of formulations and grades.
Buffer solutions serve to keep pH (acidity or alkalinity) nearly constant in a variety of chemical and biological applications. For example, a bicarbonate buffer helps maintain the pH of blood, a buffered saline maintains cellular contents at a consistent pH level, and buffers help maintain a narrow pH range for enzymes to function correctly.
Most biological samples used in research are kept at a pH of about 7.4. Common biological buffers used for tissue culture include Dulbeccos phosphate buffered saline (PBS), Tris base, HEPES, MOPS, PIPES, and other formulations developed for specific cell lines or applications. Most buffers used in cell culture are also DNase-, RNase-, and protease-free.
The choice of buffer for a particular biological reaction or site depends on several factors:
- Temperature
- Desired or target pH
- Buffer toxicity (to the system)
- Buffer interactions with other system components
Boston Bioproducts Inc MOPS Buffer (1 M, pH 7.4) - 500ml
The MOPS Buffer [3- (N-morpholino)propanesulfonic acid buffer] is a versatile zwitterionic Good's buffer used in biological research. With a pKa of 7.20, MOPS is an excellent buffer for many biological systems at near-neutral pH, and maintains a pH range from 6.5 to 7.9. It is often used as a buffering agent in biology and biochemistry, and is recommended for polyacrylamide gel electrophoresis, as a running buffer, in separating RNA in agarose gels, and for protein purification chromatography.
Sigma Aldrich Fine Chemicals Biosciences HEPES solution 1 M pH 7.0
HEPES has been described as one of the best all-purpose buffers available for biological research. At biological pH the molecule is zwitterionic and is effective as a buffer at pH 6.8 to 8.2 (pKa 7.55). It is typically used in cell culture at concentration between 5mM to 30 mM. HEPES has been used in a wide variety of applications including tissue culture. It is commonly used to buffer cell culture media in air. HEPES finds its usage in in vitro experiments on Mg.
eMolecules 117550-36-4 | Methyl (2-(Hydroxymethyl)Phenyl)Carbamate | MFCD14680132 | 250mg
AstaTech | TERT-BUTYL 4-(CYANOMETHYL)PHENYLCARBAMATE | 1g | 449717503 | 21119 | 95.000 | 1233249-35-8 | MFCD17465121 | 232.283 | C13H16N2O2
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Electron Microscopy Sciences Pipes 1.5 Sodium Salt, Crystal, 6.8 @25°C pH 100 GR
(Piperazine-N,N-bis (2-ethanesulfonic acid,) 1.5 Sodium)
F.W. 335.3
pH: 6.8 @25°C
Electron Microscopy Sciences Pipes 1.5 Sodium Salt, Crystal, 6.8 @25°C pH 25 GR
(Piperazine-N,N-bis (2-ethanesulfonic acid,) 1.5 Sodium)
F.W. 335.3
pH: 6.8 @25°C
Biotang Inc TRIS-HCL Buffer 1.0M, pH 7.8 (0.2 micron filtered), 500 ml
TRIS-HCL Buffer 1.0M, pH 7.8 (0.2 micron filtered), 500 ml.
Biotang Inc TRIS-Buffered Saline (TBS, 10X) For Western Blot Washing; (NaCl 1.5M Tris 0.5M), 1000 ml
TRIS-Buffered Saline (TBS, 10X) For Western Blot Washing; (NaCl 1.5M Tris 0.5M), 1000 ml.
Biotang Inc TRIS-HCL Buffer 1.0M, pH 8.0 (0.2 micron filtered), 500 ml
TRIS-HCL Buffer 1.0M, pH 8.0 (0.2 micron filtered), 500 ml.
Biotang Inc TRIS Buffered Saline, (20X) Tris 500 mM KCL 54 mM NaCl 2.74M, 1000 ml
TRIS Buffered Saline, (20X) Tris 500 mM KCL 54 mM NaCl 2.74M, 1000 ml.