Life Science Buffers
Balanced salt solutions and powders used for molecular, protein, nucleic acid, and cell biology applications including cell culture, electrophoresis, ELISA, and chromatography processes; available in a variety of formulations and grades.
Buffer solutions serve to keep pH (acidity or alkalinity) nearly constant in a variety of chemical and biological applications. For example, a bicarbonate buffer helps maintain the pH of blood, a buffered saline maintains cellular contents at a consistent pH level, and buffers help maintain a narrow pH range for enzymes to function correctly.
Most biological samples used in research are kept at a pH of about 7.4. Common biological buffers used for tissue culture include Dulbeccos phosphate buffered saline (PBS), Tris base, HEPES, MOPS, PIPES, and other formulations developed for specific cell lines or applications. Most buffers used in cell culture are also DNase-, RNase-, and protease-free.
The choice of buffer for a particular biological reaction or site depends on several factors:
- Temperature
- Desired or target pH
- Buffer toxicity (to the system)
- Buffer interactions with other system components
Hampton Research HR2-902-01/StockOptions HEPES #01 pH 6.8, 185 ml
HR2-902-01/StockOptions HEPES #01 pH 6.8, 185 ml. Please note items from this supplier are non-returnable.
Chem-Impex International, Inc. Ethylenediaminetetraacetic acid | 60-00-4 | MFCD00003541 | 1KG
Ethylenediaminetetraacetic acid, 60-00-4, MFCD00003541, 1KG
Dojindo Molecular Technologies Inc HEPES (100 G), Good's buffer, CAS: 7365-45-9, Purity: ≥99.0% (Titration), pKa=7.55, Active pH 6.8-8.2, made in Japan
Buffers perform an important task in biological experiments, often designed to keep pH within the 6-8 range where most biological reactions occur. Dr. Norman Good, the developer of HEPES, wanted to create buffers that prioritized this function, while also providing other benefits. Dr. Good produced a series of “Good buffers” – including HEPES (2-[4-(2-Hydroxyethyl)-1-piperazinyl]ethanesulfonic acid) – that are nearly ubiquitous in their usage even now. The Good series of buffers share the following additional characteristics: 1) High water-solubility. 2) Low cell membrane permeability. 3) Consistent acid-base dissociation constants. 4) Low metal-chelating capability. 5) High chemical stability. 6)Low absorption spectra in UV and visible regions.
![eMolecules Pharmablock / benzyl N-[1-(hydroxymethyl)-3-(1-methylcyclopropyl)propyl]carbamate / 25mg / 716543657 / PBPW703 / 0.000 / / [null] / 277.364 / C16H23NO3](https://assets.fishersci.com/TFS-Assets/CCG/product-images/502472734.JPG-250.jpg)
eMolecules Pharmablock / benzyl N-[1-(hydroxymethyl)-3-(1-methylcyclopropyl)propyl]carbamate / 25mg / 716543657 / PBPW703 / 0.000 / / [null] / 277.364 / C16H23NO3
Pharmablock / benzyl N-[1-(hydroxymethyl)-3-(1-methylcyclopropyl)propyl]carbamate / 25mg / 716543657 / PBPW703 / 0.000 / / [null] / 277.364 / C16H23NO3
Electron Microscopy Sciences D-PBS, Formula II Phosphate Buffer Saline 500 ML
All of our Dullbecco's Phosphate Buffer Saline Solutions contain Potassium Chloride, Potassium Phosphate Monobasic, Sodium Chloride, Sodium Phosphate Dibasic, and D-Glucose. Some formulations may contain additional components. Solutions are prepared 1X strength.
Electron Microscopy Sciences D-PBS, Formula II Phosphate Buffer Saline 1 LT
All of our Dullbecco's Phosphate Buffer Saline Solutions contain Potassium Chloride, Potassium Phosphate Monobasic, Sodium Chloride, Sodium Phosphate Dibasic, and D-Glucose. Some formulations may contain additional components. Solutions are prepared 1X strength.
Electron Microscopy Sciences Hank's Balanced Salts Formula I 500 ML
All of our Hanks' Balanced Salt Solutions contain: Potassium Chloride, Potassium Phosphate Monobasic, Sodium Chloride, Sodium Phosphate Dibasic, and D-Glucose. Some formulations may contain additional components. Use the table below as a quick reference for identifying the one that best suits your application. All solutions are prepared in 1X strength.
eMolecules 2,4-Dichloro-N-(2-hydroxyethyl)benzenecarboxamide | 20656-08-0 | MFCD00141691 | 1g
Matrix Scientific | 2,4-Dichloro-N-(2-hydroxyethyl)benzenecarboxamide | 1g | 389721058 | 45569 | 95.000 | 20656-08-0 | MFCD00141691 | 234.080 | C9H9Cl2NO2
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