Life Science Buffers
Balanced salt solutions and powders used for molecular, protein, nucleic acid, and cell biology applications including cell culture, electrophoresis, ELISA, and chromatography processes; available in a variety of formulations and grades.
Buffer solutions serve to keep pH (acidity or alkalinity) nearly constant in a variety of chemical and biological applications. For example, a bicarbonate buffer helps maintain the pH of blood, a buffered saline maintains cellular contents at a consistent pH level, and buffers help maintain a narrow pH range for enzymes to function correctly.
Most biological samples used in research are kept at a pH of about 7.4. Common biological buffers used for tissue culture include Dulbeccos phosphate buffered saline (PBS), Tris base, HEPES, MOPS, PIPES, and other formulations developed for specific cell lines or applications. Most buffers used in cell culture are also DNase-, RNase-, and protease-free.
The choice of buffer for a particular biological reaction or site depends on several factors:
- Temperature
- Desired or target pH
- Buffer toxicity (to the system)
- Buffer interactions with other system components
Sigma Aldrich Fine Chemicals Biosciences Piperazine BioUltra, anhydrous, >=99.0% (T) | 110-85-0 | MFCD00005953 | 25G
Piperazine BioUltra, anhydrous, >=99.0% (T) | Purity: >=99.0% (T) | Mol Wt: 86.14 | 110-85-0 | MFCD00005953 | 25G
Bioworld Bis-Tris, 100 g
Synonym: 2,2-Bis(hydroxymethyl)-2,2′,2″-nitrilotriethanol, 2-Bis(2-hydroxyethyl)amino-2-(hydroxymethyl)-1,3-propanediol, Bis(2-hydroxyethyl)amino-tris(hydroxymethyl)methane A common biological bufferAn inert, zwitterionic buffer that exhibits only a very small shift in dissociation constantSpecifications:Ultra Pure GradepKa (25°C): 6.5+/-0.2Appearance: SolidBoiling Point : 466.18 °C at 760 mmHg (Predicted)Density : 1.35 g/cm3 (Predicted)Melting Point: 102-105 °CPH Value: 5.8-7.2
Biotang Inc TRIS-HCL Buffer 1.0M, pH 8.4 (0.2 micron filtered), 500 ml
TRIS-HCL Buffer 1.0M, pH 8.4 (0.2 micron filtered), 500 ml.
Biotang Inc Tricine-buffered Saline (5X) pH 7.0, 250 ml
Tricine-buffered Saline (5X) pH 7.0, 250 ml.
Research Products International Corp RPI TRIS-CAPS Buffer 10X Solution, 2 x 500 Milliliter Bottles
This product is shipped with one 500 ml Anode bottle and one 500 ml Cathode bottle
10X Anode Formulation:
TRIS: 0.6 M
CAPS: 0.4M
10X Cathode Formulation:
TRIS: 0.6 M
CAPS: 0.4 M
SDS: 1%
Bioworld TBE Buffer w/ Sodium for Resolve Gels (1X), 20 PK(s)
Product DescriptionTBE (Tris-Borate-EDTA) buffer is for resolve gels that comes with sodium and has a concentration of 1X. Each pack has 500 mL of pre-weighed powder mix, product comes with 20 packs.For laboratory use only. Not fit for agricultural, household, clinical use or human consumption.
Sigma Aldrich Fine Chemicals Biosciences TrizmaR base Primary Stand25KG
Tris is an established basimetric standard and buffer used in biochemistry and molecular biology. It may be used by itself as a buffer or as a component of mixed buffer formulations such as Tris-EDTA (TE) buffer Tris-acetate-EDTA (TAE) buffer Tris-borate-EDTA (TBE) buffer etc. It is pure essentially stable relatively non-hygroscopic and has a high equivalent weight.
![Alkali Scientific HEPES, Sodium Salt [N (2 Hydroxyethyl) piperazine N' (2 ethanesulfonic acid)], 500 G](https://assets.fishersci.com/TFS-Assets/CCG/product-images/5000659775.jpg-250.jpg)
Alkali Scientific HEPES, Sodium Salt [N (2 Hydroxyethyl) piperazine N' (2 ethanesulfonic acid)], 500 G
HEPES, Sodium Salt is a biochemical reagent available in a 500g size, ideal for large-scale applications in biological and chemical research. It is widely used to maintain pH stability in biological systems, particularly in cell culture, protein assays, and enzyme reactions. HEPES sodium salt provides reliable buffering capacity in the pH range of 6.8–8.2, ensuring that experimental conditions remain consistent. It is non-toxic to cells, supporting reproducible results in long-term experiments. This bulk package is perfect for high-throughput laboratories, where large quantities of buffer are required for routine cell culture and biochemical analysis procedures.
![Alkali Scientific BES [N,N bis(2 Hydroxyethyl) 2 aminoethanesulfonic acid], 250 G](https://assets.fishersci.com/TFS-Assets/CCG/product-images/5000659982.jpg-250.jpg)
Alkali Scientific BES [N,N bis(2 Hydroxyethyl) 2 aminoethanesulfonic acid], 250 G
BES (N,N bis(2 Hydroxyethyl) 2 aminoethanesulfonic acid) is a biochemical reagent and buffering agent used to prepare stable pH environments in laboratory experiments. The 250g quantity is suitable for large-scale applications and long-term use in various research projects. BES is often employed in biochemical assays, including enzyme activity studies, protein analyses, and molecular biology experiments, where maintaining a stable pH is critical. It is highly valued in protocols requiring accurate and consistent pH control, such as electrophoresis and buffer preparation for biological applications. BES is indispensable for labs focused on research that demands reliable buffering for precise biochemical reactions.
eMolecules 7365-45-9 | 4-(2-Hydroxyethyl)-1-piperazineethanesulfonic acid | Chem-Impex | MFCD00006158 | 238.300 | C8H18N2O4S | 100.000 | OCCN1CCN(CCS(O)(=O)=O)CC1 | 250g | 603112820
4-(2-Hydroxyethyl)-1-piperazineethanesulfonic acid | Chem-Impex | 7365-45-9 | MFCD00006158 | 238.300 | C8H18N2O4S | 100.000 | OCCN1CCN(CCS(O)(=O)=O)CC1 | 250g | 603112820