Life Science Buffers
Balanced salt solutions and powders used for molecular, protein, nucleic acid, and cell biology applications including cell culture, electrophoresis, ELISA, and chromatography processes; available in a variety of formulations and grades.
Buffer solutions serve to keep pH (acidity or alkalinity) nearly constant in a variety of chemical and biological applications. For example, a bicarbonate buffer helps maintain the pH of blood, a buffered saline maintains cellular contents at a consistent pH level, and buffers help maintain a narrow pH range for enzymes to function correctly.
Most biological samples used in research are kept at a pH of about 7.4. Common biological buffers used for tissue culture include Dulbeccos phosphate buffered saline (PBS), Tris base, HEPES, MOPS, PIPES, and other formulations developed for specific cell lines or applications. Most buffers used in cell culture are also DNase-, RNase-, and protease-free.
The choice of buffer for a particular biological reaction or site depends on several factors:
- Temperature
- Desired or target pH
- Buffer toxicity (to the system)
- Buffer interactions with other system components
Teknova 50mM MES 1M Sodium Chloride
50mM MES, 1M Sodium Chloride, pH 6.0, 1000mL, Endotoxin Tested, Sterile. 8 pack.
Chem-Impex International, Inc. 3-Cyclohexylamino-1-propanesulfonic acid | 1135-40-6 | MFCD00003837 | 100G
3-Cyclohexylamino-1-propanesulfonic acid, 1135-40-6, MFCD00003837, 100G
Teknova 1X PBS Solution, pH 7.4, 20L Bag, Sterile. 4 Pack.
Phosphate-buffered saline (PBS) is a salt solution that is commonly used in physiological and biochemical experiments. It can be used to maintain pH neutrality or to buffer against changes in pH in order to provide optimal conditions for cellular growth and function. The buffering range is between pH 6.8 and 7.4.
Teknova 1M Tris-HCl, pH 9.0 Solution, 20L Bag, Sterile. 4 Pack.
Tris (tris(hydroxymethyl)aminomethane) is used as a component ofbuffer solutions, especially for solutions ofnucleic acids. The useful buffer range for tris (pH 7–9) is similar to the pH of most living organisms. This, and its low cost, make tris one of the most common buffers in the biology/biochemistry laboratory.
Gold Biotechnology Inc Bis-Tris 1 kg
Bis-Tris, also known as bis-Tris methane, is a zwitterionic buffering agent used in biochemistry and molecular biology. It is a bis(2-hydroxyethyl)amine and Tris family buffer that is useful for a pH range of 5.8 – 7.2. Bis Tris is a common component of many buffer systems for various types of electrophoresis. Bis-Tris may form a complex with some common metals, such as Cu(II) and Pb(II), and formation constants should be taken into account when using this buffer in a solution containing metal ions. Bis-Tris can be used as a substitute for the highly toxic buffer cacodylate.
Teknova Elution Solution-10mM Tris-HCl pH 8.5, 0.01% Tween-20. 1000mL. DNAse Tested. Sterile. 8 pack.
Elution Solution-10mM Tris-HCl pH 8.5, 0.01% Tween-20. 1000mL. DNAse Tested. Sterile. 8 pack.
![Research Products International Corp HEPPS [N-(2-Hydroxyethyl)piperazine-N'-(3-propane sulfonic acid)] [EPPS], 100 Grams](https://assets.fishersci.com/TFS-Assets/CCG/product-images/VN00006095-H75060-100.0.JPG-250.jpg)
Research Products International Corp HEPPS [N-(2-Hydroxyethyl)piperazine-N'-(3-propane sulfonic acid)] [EPPS], 100 Grams
- Substance Name: HEPPS [N-(2-Hydroxyethyl)piperazine-N'-(3-propane sulfonic acid)] [EPPS]
- CAS Number: 16052-06-5
- Molecular Formula: C9H20N2O4S
- Molecular Weight: 252.3
Medchemexpress LLC Nvp-tae 226 | 761437-28-9 | MFCD12031516 | 99.8% | 468.94 g·mol⁻¹ | C23H25ClN6O3 | 5 MG
NVP-TAE 226 is an ATP-competitive small-molecule inhibitor of focal adhesion kinase (FAK) and insulin-like growth factor 1 receptor (IGF-1R), used in cellular and preclinical cancer research to probe signaling pathways and evaluate effects on proliferation, migration, and survival.
- Potent inhibition of FAK and Pyk2 with low-nanomolar IC50s.
- Also inhibits IGF-1R and insulin receptor, enabling study of connected signaling networks.
- Suitable for in vitro assays and preclinical efficacy models.
- Available as solid and solution formulations for flexible dosing.
- High purity appropriate for research applications.
- Stable under recommended storage conditions for prolonged shelf life.
Hampton Research HR2-902-15/StockOptions HEPES #15 pH 8.2, 185 ml
HR2-902-15/StockOptions HEPES #15 pH 8.2, 185 ml. Please note items from this supplier are non-returnable.
Nusep Inc NUVIEW MINI GEL 4-20 12-WELL
NuSep's nUView Tris-Glycine SDS-PAGE Precast Gel 4-20% 12well. Cassette size: 10 x 8.5 cm. Fits Mini-PRO or Bio-Rad's Mini-Protean Tetra cell Tanks. 10 gel cassettes per box. All nUView™ precast gels incorporate a unique formulation allowing protein bands to be visualized in only 2 minutes in the presence of ultraviolet (UV) light. Simply place your gel over a 250-320 nm UV trans-illuminator and watch high-resolution bands appear in just 2 minutes. With nUView™ technology you no longer have to stain and de-stain your gels – increasing protein recovery and saving you significant time while receiving improved results.
Medchemexpress LLC Daratumumab PBS 1mg | 1mg
Daratumumab (PBS) (Anti-Human CD38) is the first-in-class human-specific anti-CD38 monoclonal antibody (IgG1) Daratumumab (PBS) has anti-multiple myeloma (MM) effect Daratumumab (PBS) impairs MM cell adhesion which results in an increased sensitivity of MM to proteasome inhibition[1][2]