Life Science Buffers
Balanced salt solutions and powders used for molecular, protein, nucleic acid, and cell biology applications including cell culture, electrophoresis, ELISA, and chromatography processes; available in a variety of formulations and grades.
Buffer solutions serve to keep pH (acidity or alkalinity) nearly constant in a variety of chemical and biological applications. For example, a bicarbonate buffer helps maintain the pH of blood, a buffered saline maintains cellular contents at a consistent pH level, and buffers help maintain a narrow pH range for enzymes to function correctly.
Most biological samples used in research are kept at a pH of about 7.4. Common biological buffers used for tissue culture include Dulbeccos phosphate buffered saline (PBS), Tris base, HEPES, MOPS, PIPES, and other formulations developed for specific cell lines or applications. Most buffers used in cell culture are also DNase-, RNase-, and protease-free.
The choice of buffer for a particular biological reaction or site depends on several factors:
- Temperature
- Desired or target pH
- Buffer toxicity (to the system)
- Buffer interactions with other system components
Chem-Impex International, Inc. Piperazine-N,N'-bis(2-ethanesulfonic acid) 1.5 sodium salt | 100037-69-2 | MFCD00066393 | 250G
Piperazine-N,N'-bis(2-ethanesulfonic acid) 1.5 sodium salt, 100037-69-2, MFCD00066393, 250G
Boston Bioproducts Inc Tris-HCl Buffer (1 M, pH 6.5) - 1L
The Tris-HCl Buffer is a widely used bufering reagent in molecular biology and biochemistry, acting to maintain a stable pH, and preserve the integrity of target macromolecules. The buffering capacity of Tris-HCl is effective in the pH range of 7.0 to 9.0, making it ideal for many biological applications like cell culture, molecular biology, DNA/RNA extraction, electrophoresis, enzyme assays and immunohistostaining.
Crystalgen Inc PBS (PHOSPHATE BUFFERED SALINE
PBS (Phosphate Buffered Saline) pH7.4, 10X, with Ca, Mg-4X1L
Sigma Aldrich Fine Chemicals Biosciences mPAGER 4-12 Bis-Tris Preca
mPAGE 4-12% Bis-Tris Precast Gel 10x8 cmUnless otherwise stated in our catalog or other company documentation accompanying the product(s) our products are intended for research use only and are not to be used for any other purpose which includes but is not limited to unauthorized commercial uses in vitro diagnostic uses ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.
Boston Bioproducts Inc Phosphate Buffered Saline, PBS (10X, pH 7.4) - 1L
Phosphate Buffered Saline (PBS) is a versatile buffer solution widely used due to its isotonic and non-toxic nature to cells. With a pH range of 7.0 to 7.4, PBS mimics physiological osmolarity and ion concentrations, making it ideal for applications including protein purification, Western blotting and immunoassays, sample dilution, and as a transport medium. Packaged as a 10X concentration, this solution can be diluted to support its application.
Chem-Impex International, Inc. N-tris(Hydroxymethyl)methyl-2-aminoethane-sulfonic acid | MFCD00007532 | 250G
N-tris(Hydroxymethyl)methyl-2-aminoethane-sulfonic acid, MFCD00007532, 250G
Research Products International Corp TRIS-Tricine-SDS Running Buffer 10X Solution, 500 ML
10X Formulation:
Tricine: 1 M
SDS (pH 8.3): 1%
Research Products International Corp HEPES Buffered Saline Solution 2X, 250 Milliliters
2X Formulation:
KCI: 10 mM
Na2HPO4: 1.5 mM
Dextrose: 12 mM
HEPES: 50 mM
pH: 7.0
INNOVAPREP LLC ELUTION FLUID CAN - PBS
FluidPrep Elution Fluid Can - PBS0.075% Tween 20/PBS. Recommended for classical analytical methods.1 can.Each can provides up to 30 extractions.
BioMed Diagnostics An isotonic solution typically used for detection of the severe acute respiratory syndrome SARS-CoV-2. | Available in: 50 pack
This Saline Solution (0.85%) has been validated for the detection of the severe acute respiratory syndrome coronavirus 2 (SARS-CoV-2) that causes COVID-19 disease in humans. | Product Principle: 1.) Intended to aid in the process of collection and transport of human clinical specimen. 2.) Ensures a non-replicating competent status of SARS-CoV-2. 3.) Is aseptic and does not contain bacteria or fungi growth-inhibition agents. 4.) Validated to be compatible with the identification of SARS-CoV-2 using qRT-PCR technology | Detects: Severe acute respiratory syndrome coronavirus 2 (SARS-CoV-2). | Available in: 50 pack
S2 Media 1M Tris-HCl, pH 8.0, Sterile, 1L Media Bottle, 1ea
1M Tris-HCl Buffer (sterile) is commonly used in labs for pH stabilization in biochemical reactions, as a buffer in analytical chemistry, and as a sample diluent near neutral to slightly basic pH. At pH 8.0, Tris (pKa ≈ 8.1 at 25°C) provides effective buffering between pH 7–9, making it compatible with most biological systems and minimally reactive with biomolecules.• 1M Tris-HCl should be used by trained professionals within a laboratory setting. • Tris is an abbreviation for tris(hydroxymethyl)aminomethane).• Product is not intended for use in the diagnosis or treatment of disease or other human conditions.• For Research Use Only (RUO). Not for use in diagnostic procedures.