Life Science Buffers
Balanced salt solutions and powders used for molecular, protein, nucleic acid, and cell biology applications including cell culture, electrophoresis, ELISA, and chromatography processes; available in a variety of formulations and grades.
Buffer solutions serve to keep pH (acidity or alkalinity) nearly constant in a variety of chemical and biological applications. For example, a bicarbonate buffer helps maintain the pH of blood, a buffered saline maintains cellular contents at a consistent pH level, and buffers help maintain a narrow pH range for enzymes to function correctly.
Most biological samples used in research are kept at a pH of about 7.4. Common biological buffers used for tissue culture include Dulbeccos phosphate buffered saline (PBS), Tris base, HEPES, MOPS, PIPES, and other formulations developed for specific cell lines or applications. Most buffers used in cell culture are also DNase-, RNase-, and protease-free.
The choice of buffer for a particular biological reaction or site depends on several factors:
- Temperature
- Desired or target pH
- Buffer toxicity (to the system)
- Buffer interactions with other system components
Research Products International Corp TAPS, 100 G
pKa(25°:C): 8.4. The working pH range in aqueous solution is 7.7-9.1.
Alkali Scientific ADA, [N (Carbamoylmethyl) iminodiacetic acid), 25 G
ADA (N-(Carbamoylmethyl) iminodiacetic acid) is a biochemical reagent available in a 25g bottle. This buffering agent is ideal for laboratory applications requiring precise pH regulation. ADA is commonly used in chromatography, protein studies, and enzyme assays, providing a stable pH environment during various biochemical experiments. Its ability to maintain a controlled pH is essential for accurate results in protein purification, cell culture, and molecular biology studies. The 25g size is perfect for smaller-scale experiments or routine laboratory use where reliable buffering capacity is necessary for optimal results.
![Medchemexpress LLC (3-{[Tris(hydroxymethyl)methyl]amino}propane-1-sulfonic acid) | 29915-38-6 | 98.0% | 50 G](https://assets.fishersci.com/TFS-Assets/CCG/product-images/5000409966-logo.png-250.jpg){kind=logo}
Medchemexpress LLC (3-{[Tris(hydroxymethyl)methyl]amino}propane-1-sulfonic acid) | 29915-38-6 | 98.0% | 50 G
TAPS is an aminosulfonate-based lysozyme stabilizer and connexin channel inhibitor. It helps maintain the native structure of lysozyme in aqueous solution at pH 7.0 and protects it from heat-induced denaturation. TAPS is also considered a critical compound for investigating the structure and function of connexin channels.
- Maintains native structure of lysozyme in aqueous solution at pH 7.0
- Protects lysozyme from heat-induced denaturation
- Directly inhibits heteromeric connexin 32/connexin 26 channels (Cx32/Cx26)
- Reduces connexin channel-mediated solute exchange in a recombinant liposome system
- Critical compound for investigating connexin channel structure and function
Dojindo Molecular Technologies Inc MES (100G), Good's buffer, CAS: 145224-94-8, pka=6.15, Active pH 5.5-7, made in Japan
In biological experiments, it is important to maintain the pH of the solutions used. Buffers, which are mixtures of weak acids and their appropriate conjugate bases, are commonly used in these experiments. Most biological reactions occur when the buffer is at its effective pH range of 6 to 8. Furthermore, the acids and bases used in the biological reaction should not produce chelates with metal ions. For these reasons, Dr. Good developed several aminoethane and aminopropane sulfonic acids that are now widely used for biological research and analysis. Goods buffers have the following characteristics: 1) High water-solubility. 2) Low cell membrane permeability. 3) Consistent acid-base dissociation constants. 4) Low metal chelating capability. 5) High chemical stability. 6) Low absorption spectra in UV and visible regions.
eMolecules 1596117-29-1 | Ambeed | (4-(6-Methyl-1245-tetrazin-3-yl)phenyl)methanamine hydrochloride | 1g | 666597771 | A1152138 | 237.69 | C10H12ClN5
Ambeed | 6-(Trifluoromethyl)picolinamide | 5g | 601097449 | A513445 | 22245-84-7 | MFCD02180820 | 190.125 | C7H5F3N2O
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Medchemexpress LLC HEPPSO | 68399-78-0 | 99.9% | 25 G
HEPPSO is a zwitterionic buffer designed for various research applications. It operates within a working pH range of 7.1-8.5 and exhibits a relatively high ability to bind copper(II). With a pKa of 7.84 at a 2.0 mM buffer concentration, it is suitable for use in laboratory settings.
- Zwitterionic buffer
- Working pH range of 7.1-8.5
- High ability to bind copper(II)
- pKa of 7.84 at 2.0 mM buffer concentration
- For research use only
![Research Products International Corp BES [N,N-bis(2-Hydroxyethyl)-2-aminoethanesulfonic acid], 25 Grams](https://assets.fishersci.com/TFS-Assets/CCG/product-images/VN00006095-B32070-25.02.JPG-250.jpg)
Research Products International Corp BES [N,N-bis(2-Hydroxyethyl)-2-aminoethanesulfonic acid], 25 Grams
For use in preparing electrophoresis buffers. pKa (25° C) 7.1. The working pH Range in aqueous solution is 6.4-7.8
Medchemexpress LLC 4-Morpholineethanesulfonic acid, sodium salt (2:1) | 117961-21-4 | 98.0% | 25 G
MES (2-Morpholinoethanesulphonic acid) hemisodium is an amphoteric ion buffer with a buffer capacity ranging from pH 5.5 to 7.0. It is a Good's buffer widely used in biochemistry and molecular biology experiments.
- Amphoteric ion buffer.
- Buffer capacity ranging pH 5.5-7.0.
- Widely used in biochemistry and molecular biology experiments, such as cell culture, enzyme activity determination, electrophoresis, and protein studies.
- Zwitterionic buffer, providing effective buffering capacity around pH 6.15.
- pKa value changes with temperature, requiring consideration for different temperatures.
- Shows good buffering properties in biological experiments.
- Exhibits poor affinity with metal ions (binding constants at 20 °C and 0.1 M: Mg2+, 0.8; Ca2+, 0.8; Mn2+, 1.7), making it suitable for studies involving metal ions.
![Research Products International Corp RPI PIPES [Piperazine-N-N'-bis(2-ethanesulfonic acid)], 5 Kilograms](https://assets.fishersci.com/TFS-Assets/CCG/product-images/VN00006095-P40140-5000.0.JPG-250.jpg)
Research Products International Corp RPI PIPES [Piperazine-N-N'-bis(2-ethanesulfonic acid)], 5 Kilograms
Heavy metals: <10 ppm
pka (25°C): 6.8
The working pH range in aqueous solution is 6.5-7.5
Medchemexpress LLC Tapso | 68399-81-5 | 98.0% | 100 G
TAPSO is a widely used biological buffer and pH stabilization reagent. It offers an effective pH range between 7 and 8, with pKa values typically falling between 7.5 and 9.0. This compound contains Tris groups and has been observed to exhibit reactive activity with zwitterionic glycine peptides. It is primarily intended for research applications.
- Effective pH range of 7-8.
- pKa values ranging from 7.5-9.0.
- Contains Tris groups.
- Shows reactive activity with zwitterionic glycine peptides.
- Suitable for various research applications.
Bioworld BES (2-(Bis(2-hydroxyethyl) amino) ethanesulfonic acid) Buffer, 0.5M, pH 7.0, 100 mL
Synonym:N,N-Bis(2-hydroxyethyl)-2-aminoethanesulfonic acid sodium salt, BES sodium saltBelongs to the class of Good buffers, as described in the research of Dr. Norman Good et al. (1966), optimized for molecular biology and biochemistry applications. The characteristics of a Good′s buffer include: pKa between 6.0 and 8.0, high solubility, non toxic, limited effect on biochemical reactions, very low absorbance between 240-700 nm, enzymatic and hydrolytic stability, minimal changes due to temperature and concentration, limited effects due to ionic or salt composition of the solution, limited interaction with mineral cations and limited permeability of biological membranes.Technical Information :Useful pH range :6.4 - 7.8pKa (25 °C) :7.1 (Free acid)Warning:For laboratory use only.Not fit for agricultural, household, clinical use or human consumption.
Research Products International Corp DPBS 1X Solution, 500 Milliliters
Phosphate Buffered Saline is a buffer solution commonly used in biological research. PBS has many uses because it is isotonic and non-toxic to most cells. These uses include substance dilution and cell container rinsing.
Ready to use sterile 1X solution.
Ingredients:
Potassium Chloride: 2.7 mM
Phosphate Buffer: 11.9 mM
pH: 7.4
Does not contain Calcium or Magnesium