Life Science Buffers
Balanced salt solutions and powders used for molecular, protein, nucleic acid, and cell biology applications including cell culture, electrophoresis, ELISA, and chromatography processes; available in a variety of formulations and grades.
Buffer solutions serve to keep pH (acidity or alkalinity) nearly constant in a variety of chemical and biological applications. For example, a bicarbonate buffer helps maintain the pH of blood, a buffered saline maintains cellular contents at a consistent pH level, and buffers help maintain a narrow pH range for enzymes to function correctly.
Most biological samples used in research are kept at a pH of about 7.4. Common biological buffers used for tissue culture include Dulbeccos phosphate buffered saline (PBS), Tris base, HEPES, MOPS, PIPES, and other formulations developed for specific cell lines or applications. Most buffers used in cell culture are also DNase-, RNase-, and protease-free.
The choice of buffer for a particular biological reaction or site depends on several factors:
- Temperature
- Desired or target pH
- Buffer toxicity (to the system)
- Buffer interactions with other system components
Medchemexpress LLC Bicine | 150-25-4 | 98.7% | 25 G
Bicine is a buffer utilized in physiological research. It is a derivative of the amino acid glycine that enhances the sensitivity of guanosine enzyme histochemical detection.
- Buffer utilized in physiological research
- Derivative of the amino acid glycine
- Enhances the sensitivity of guanosine enzyme histochemical detection
Medchemexpress LLC MOPS (sodium salt) | 71119-22-7 | 99.8% | 25 G
MOPS sodium salt is a buffering agent utilized in biological applications. MOPS buffer is effective in maintaining the pH of mammalian cell culture media and has been shown to significantly improve the thermal stability of BSA. It is relatively safe for short-term experiments, though it may reduce cell viability after 4 hours, suggesting experimental duration limits of 4-12 hours.
- Buffering agent for biological research.
- Maintains pH in mammalian cell culture media.
- Significantly improves the thermal stability of BSA.
- Relatively safe for short-term experiments, though it may reduce cell viability after 4 hours, suggesting experimental duration limits of 4-12 hours.
- Can be used in the preparation of keratinocyte and microbial culture media.
![Medchemexpress LLC 1-Propanesulfonic acid, 2-hydroxy-3-[[2-hydroxy-1,1-bis(hydroxymethyl)ethyl]amino]- | 68399-81-5 | 98.0% | 25 G](https://assets.fishersci.com/TFS-Assets/CCG/product-images/5000414468-logo.png-250.jpg){kind=logo}
Medchemexpress LLC 1-Propanesulfonic acid, 2-hydroxy-3-[[2-hydroxy-1,1-bis(hydroxymethyl)ethyl]amino]- | 68399-81-5 | 98.0% | 25 G
TAPSO is a common biological buffer and pH stabilization reagent. It has an effective pH ranging from 7-8 and pKa values ranging from 7.5-9.0. This compound contains Tris groups and exhibits reactive activity with zwitterionic glycine peptides.
- Common biological buffer
- pH stabilization reagent
- Effective pH ranging from 7-8
- pKa values ranging from 7.5-9.0
- Contains Tris groups
- Reactive with zwitterionic glycine peptides
- Suitable for research applications
Sigma Aldrich Fine Chemicals Biosciences PIPES BioXtra, for molecular biology, >=99.5% (T) | 5625-37-6 | MFCD00006159 | 50G
PIPES BioXtra, for molecular biology, >=99.5% (T) | Purity: >=99.5% (T) | Mol Wt: 302.37 | 5625-37-6 | MFCD00006159 | 50G
Biotang Inc TRIS-Glycine-SDS Running Buffer (10X), 1000 ml
TRIS-Glycine-SDS Running Buffer (10X), 1000 ml.
Medchemexpress LLC HEPES | 7365-45-9 | 99.9% | 1 G
HEPES is a nonvolatile zwitterionic chemical buffering agent broadly applied in cell culture. It is effective at pH 6.8 to 8.2 and is also a potent inducer of lysosome biogenesis.
- Nonvolatile zwitterionic chemical buffering agent
- Broadly applied in cell culture
- Effective at pH 6.8 to 8.2
- Potent inducer of lysosome biogenesis
Quality Biological Inc Tris (1 M) pH 9.8 1000mL
Suitable for molecular biology protein chemistry and biochemical applications.DNase RNase and Protease tested.Filtered through a 0.22 micron membraneDnase Rnase and Protease tested Manufactured in an ISO 90012015 certified facility.
Charles River Laboratories 4 mL 0.5 M MgSO4, 1 M Tris buffer, 6/pk
4 mL 0.5 M MgSO4, 1 M Tris buffer, 6/pk.
![Research Products International Corp RPI MOPS [3-(N-morpholino)-propanesulfonic acid], 10 Kilograms](https://assets.fishersci.com/TFS-Assets/CCG/product-images/VN00006095-M92020-10000.0.JPG-250.jpg)
Research Products International Corp RPI MOPS [3-(N-morpholino)-propanesulfonic acid], 10 Kilograms
MOPS, or 3-(N-Morpholino) propanesulfonic acid, is a zwitterionic buffer. MOPS is primarily utilized as a buffering agent to maintain a stable pH in various experimental procedures. It is particularly effective in the pH range of 6.5 to 7.9, making it suitable for applications requiring slightly acidic to neutral pH conditions.
MOPS is commonly used in protein purification, enzyme assays, nucleic acid research, and electrophoresis techniques where a specific pH range needs to be maintained. Its buffering capacity and stability within the desired pH range make it valuable in these applications.
One of the significant advantages of MOPS is its compatibility with biological systems and enzymes. It exhibits minimal interference with enzymatic activity and other biological processes, ensuring reliable and accurate results.
pH Range: 6.5-7.9
Boston Bioproducts Inc Tris-HCl Buffer (1 M, pH 7.6) - 500ml
The Tris-HCl Buffer is a widely used bufering reagent in molecular biology and biochemistry, acting to maintain a stable pH, and preserve the integrity of target macromolecules. The buffering capacity of Tris-HCl is effective in the pH range of 7.0 to 9.0, making it ideal for many biological applications like cell culture, molecular biology, DNA/RNA extraction, electrophoresis, enzyme assays and immunohistostaining.
eMolecules 502650-56-8 | methyl cis-3-(hydroxymethyl)cyclohexanecarboxylate | 1g
Pharmablock - Stock | methyl cis-3-(hydroxymethyl)cyclohexanecarboxylate | 1g | 534153256 | PBJL037 | 502650-56-8 | 172.224 | C9H16O3
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Sigma Aldrich Fine Chemicals Biosciences Dulbeccos Phosphate Bufer500ML
DULBECCOS Dulbeccos Phosphate Bufer500ML
Boston Bioproducts Inc Tris Buffered Saline, TBS (10X, pH 7.4) - 4L
The Tris Buffered Saline (TBS) is an isotonic non-toxic buffer solution used commonly in biochemical immunoassay as a wash buffer. Tris maintains a stable pH range typically between 7.4 to 8.0 while NaCl regulates osmotic balance via its elected concentration. Tris maintains a stable pH range typically between 7.4 to 8.0 while NaCl regulates osmotic balance via its elected concentration. This TBS Solution is formulated at a 10X concentration.