Life Science Buffers
Balanced salt solutions and powders used for molecular, protein, nucleic acid, and cell biology applications including cell culture, electrophoresis, ELISA, and chromatography processes; available in a variety of formulations and grades.
Buffer solutions serve to keep pH (acidity or alkalinity) nearly constant in a variety of chemical and biological applications. For example, a bicarbonate buffer helps maintain the pH of blood, a buffered saline maintains cellular contents at a consistent pH level, and buffers help maintain a narrow pH range for enzymes to function correctly.
Most biological samples used in research are kept at a pH of about 7.4. Common biological buffers used for tissue culture include Dulbeccos phosphate buffered saline (PBS), Tris base, HEPES, MOPS, PIPES, and other formulations developed for specific cell lines or applications. Most buffers used in cell culture are also DNase-, RNase-, and protease-free.
The choice of buffer for a particular biological reaction or site depends on several factors:
- Temperature
- Desired or target pH
- Buffer toxicity (to the system)
- Buffer interactions with other system components
Quality Biological Inc TBS (10X), pH 7.4, 10 Liters
TBS (10X), pH 7.4 is used as a washing buffer for cellular and molecular biology techniques.
DNase, RNase and Protease tested.
Applications: TBS (10X), pH 7.4 will be useful as a washing buffer for electrodes, microtiter plates, and alkaline phophatase or peroxidase conjugates. TBS (10X), pH 7.44 can also be used in detergent preparation and Western blot or ELISA applications.
Storage Conditions: 15 °C to 30 °C
Shipping Condition: ambient temperature
Sterile Filtered
DNase, RNase, and Protease Tested
pH: 7.4 ± 0.1
Max Shelf Life: 36 months from date of manufacture
Product and/or packaging contains recycled/renewable content. Product and/or packaging is recyclable or reduces waste. Product and/or packaging is safer to human and environmental health.
Quality Biological Inc TAE Buffer (50X), 10 Liters
TAE (Tris-Acetate EDTA Buffer) buffer provides faster electrophoretic migration of linear DNA and better resolution of supercoiled DNA. TAE Buffer may be more suitable than TBE, for electrophoresis if DNA is to be recovered from the agarose and purified using commercially available DNA purification kits.
Applications: TAE buffer has a relatively low buffering capacity, therefore, periodic replacement of the buffer during prolonged electrophoresis is recommended.
Storage Conditions: 15 °C to 30 °C
Shipping Condition: ambient temperature
Sterile Filtered
DNase RNase and Protease Tested
Concentration: 2M Tris base and 0.05M disodium EDTA dihydrate
Max Shelf Life: 36 months from date of manufacture
Product and/or packaging contains recycled/renewable content. Product and/or packaging is recyclable or reduces waste. Product and/or packaging is safer to human and environmental health.
Quality Biological Inc TBS (10X), pH 7.4, 4 Liters
TBS (10X), pH 7.4 is used as a washing buffer for cellular and molecular biology techniques.
DNase, RNase and Protease tested.
Applications: TBS (10X), pH 7.4 will be useful as a washing buffer for electrodes, microtiter plates, and alkaline phophatase or peroxidase conjugates. TBS (10X), pH 7.44 can also be used in detergent preparation and Western blot or ELISA applications.
Storage Conditions: 15 °C to 30 °C
Shipping Condition: ambient temperature
Sterile Filtered
DNase, RNase, and Protease Tested
pH: 7.4 ± 0.1
Max Shelf Life: 36 months from date of manufacture
Product and/or packaging contains recycled/renewable content. Product and/or packaging is recyclable or reduces waste. Product and/or packaging is safer to human and environmental health.
Quality Biological Inc PBS (10X), pH 7.4, 4 Liters
PBS is a balanced salt solution used for washing cells before dissociation, transporting cells or tissue, diluting cells for counting, and preparing reagents. Useful in immunological applications, such as Western Blotting.
Without Calcium and Magnesium
Endotoxin and Mycoplasma tested
Storage Condition: 15 °C to 30 °C
Shipping Condition: ambient temperature
Sterile Filtered
Endotoxin and Mycoplasma Tested
Osmolality: 300 ± 55 mOsm/Kg H20
Max Shelf Life: 24 months from date of manufacture
Manufactured under an ISO 9001:2015 certified quality management system.
Product and/or packaging contains recycled/renewable content. Product and/or packaging is recyclable or reduces waste. Product and/or packaging is safer to human and environmental health.
Quality Biological Inc PBS (10X), pH 7.4, 10 Liters
PBS is a balanced salt solution used for washing cells before dissociation, transporting cells or tissue, diluting cells for counting, and preparing reagents. Useful in immunological applications, such as Western Blotting.
Without Calcium and Magnesium
Endotoxin and Mycoplasma tested
Storage Condition: 15 °C to 30 °C
Shipping Condition: ambient temperature
Sterile Filtered
Endotoxin and Mycoplasma Tested
Osmolality: 300 ± 55 mOsm/Kg H20
Max Shelf Life: 24 months from date of manufacture
Manufactured under an ISO 9001:2015 certified quality management system.
Product and/or packaging contains recycled/renewable content. Product and/or packaging is recyclable or reduces waste. Product and/or packaging is safer to human and environmental health.
Quality Biological Inc TE, pH 7.5, 1000 mL
TE is composed of buffering agent Tris and chelating agent EDTA. EDTA prevents degradation of DNA and RNA by chelating magnesium (or other divalent metal ions). Together, this Tris-EDTA based solution solubilizes DNA or RNA, while protecting it from degradation.
Applications: TE is useful for reconstituting nucleic acids, during DNA precipitation and other DNA applications, storing and diluting DNA and RNA, breaking protein-links in immuno-histochemistry procedures, and unmasking antigens and epitopes in formalin-fixed and paraffin-embedded tissue sections.
Storage Conditions: 15 °C to 30 °C
Shipping Condition: ambient temperature
Sterile Filtered
DNase, RNase and Protease Tested
Max Shelf Life: 36 months from date of manufacture
Product and/or packaging contains recycled/renewable content. Product and/or packaging is recyclable or reduces waste. Product and/or packaging is safer to human and environmental health.
Quality Biological Inc DPBS (10X) without Calcium and Magnesium, 10 Liters
DPBS is a balanced salt solution used for the handling and culturing of mammalian cells. DPBS without calcium and magnesium can be used to wash and rinse suspended cells. irrigating, transporting or diluting fluid for mammalian cells, while maintaining cell tonicity and viability for a limited period of time.
Without Calcium and Magnesium
Without Phenol Red
Storage Condition: 15 °C to 30 °C
Shipping Condition: ambient temperature
Sterile Filtered
Endotoxin and Mycoplasma Tested
Max Shelf Life: 24 months from date of manufacture
Manufactured under an ISO 9001:2015 certified quality management system.
Product and/or packaging contains recycled/renewable content. Product and/or packaging is recyclable or reduces waste. Product and/or packaging is safer to human and environmental health.
Quality Biological Inc PBS (10X), pH 7.4, 20 Liters
PBS is a balanced salt solution used for washing cells before dissociation, transporting cells or tissue, diluting cells for counting, and preparing reagents. Useful in immunological applications, such as Western Blotting.
Without Calcium and Magnesium
Endotoxin and Mycoplasma tested
Storage Condition: 15 °C to 30 °C
Shipping Condition: ambient temperature
Sterile Filtered
Endotoxin and Mycoplasma Tested
Osmolality: 300 ± 55 mOsm/Kg H20
Max Shelf Life: 24 months from date of manufacture
Manufactured under an ISO 9001:2015 certified quality management system.
Product and/or packaging contains recycled/renewable content. Product and/or packaging is recyclable or reduces waste. Product and/or packaging is safer to human and environmental health.
Quality Biological Inc PBS (1X), pH 7.4 without Calcium and Magnesium, 10 Liters
PBS (phosphate buffered saline) Balanced salt solution used for a variety biological and cell culture applications, such as washing cells before dissociation, transporting cells or tissue, diluting cells for counting, and preparing reagents. Also useful for immunological applications, such as Western Blotting.
Endotoxin and Mycoplasma tested
Without Calcium and Magnesium
Storage Condition: 15 °C to 30 °C
Shipping Condition: ambient temperature
Sterile Filtered
Endotoxin and Mycoplasma Tested
pH: 7.3 7.5
Osmolality: 300 ± 5% mOsm/Kg H2O
Max Shelf Life: 24 months from date of manufacture
Manufactured under an ISO 9001:2015 certified quality management system.
Product and/or packaging contains recycled/renewable content. Product and/or packaging is recyclable or reduces waste. Product and/or packaging is safer to human and environmental health.
Quality Biological Inc DPBS without Calcium and Magnesium, 10 Liters
DPBS is a balanced salt solution (BSS) ¿ Used for the handling and culturing of mammalian cells. ¿ Calcium and magnesium facilitate cell binding and clumping. ¿ DPBS without these ions can be used to wash and rinse suspended cells. ¿ Applications: DPBS serves as an irrigating, transporting or diluting fluid for mammalian cells, while maintaining cell tonicity and viability for a limited period of time. ¿ Used as a diluent of fresh red blood cells to study the trapping of red blood cell aggregate Liters ¿ Wash the wells of microtiter plates ¿ Wash cells during preparation and serial transfer. ¿ Storage Condition: 15¿C to 30¿C ¿ Shipping Condition: ambient temperature ¿ Sterile Filtered ¿ Product and/or packaging contains recycled/renewable content ¿ Product and/or packaging is recyclable or reduces waste ¿ Product and/or packaging is safer to human and environmental health ¿ UNSPSC: 12161700 ¿ ¿ Max Shelf Life: 24 months
Sigma Aldrich Fine Chemicals Biosciences Ethylenediaminetetraacetic acid BioUltra, anhydrous | 60-00-4 | MFCD00003541 | 500g
Ethylenediaminetetraacetic acid BioUltra, anhydrous | Purity: 99% | Mol Wt: 292.24 | 60-00-4 | MFCD00003541 | 500g
Sigma Aldrich Fine Chemicals Biosciences Ethylenediaminetetraacetic acid ACS reagent, 99.4-100.6%, powder | 60-00-4 | MFCD00003541 | 1KG
Ethylenediaminetetraacetic acid ACS reagent, 99.4-100.6%, powder | Purity: 99.4-100.6% | Mol Wt: 292.24 | 60-00-4 | MFCD00003541 | 1KG
Research Products International Corp MES, Anhydrous, 100 Grams
- CAS Number: 4432-31-9
- Chemical Formula: C6H13NO4S
- Molecular Weight: 195.24 g/mol
![Research Products International Corp TAPS, [Tris(hydroxymethyl)-3-aminopropanesulfonic acid], Free Acid, 500 Grams](https://assets.fishersci.com/TFS-Assets/CCG/product-images/VN00006095-T12000-500.0.JPG-250.jpg)
Research Products International Corp TAPS, [Tris(hydroxymethyl)-3-aminopropanesulfonic acid], Free Acid, 500 Grams
pKa(25°:C): 8.4. The working pH range in aqueous solution is 7.7-9.1.
Sigma Aldrich Fine Chemicals Biosciences MOPS hemisodium salt >=99% (titration) | 117961-20-3 | 250G
MOPS hemisodium salt >=99% (titration) | Purity: >=99% (titration) | Mol Wt: 220.25 | 117961-20-3 | 250G