Life Science Buffers
Balanced salt solutions and powders used for molecular, protein, nucleic acid, and cell biology applications including cell culture, electrophoresis, ELISA, and chromatography processes; available in a variety of formulations and grades.
Buffer solutions serve to keep pH (acidity or alkalinity) nearly constant in a variety of chemical and biological applications. For example, a bicarbonate buffer helps maintain the pH of blood, a buffered saline maintains cellular contents at a consistent pH level, and buffers help maintain a narrow pH range for enzymes to function correctly.
Most biological samples used in research are kept at a pH of about 7.4. Common biological buffers used for tissue culture include Dulbeccos phosphate buffered saline (PBS), Tris base, HEPES, MOPS, PIPES, and other formulations developed for specific cell lines or applications. Most buffers used in cell culture are also DNase-, RNase-, and protease-free.
The choice of buffer for a particular biological reaction or site depends on several factors:
- Temperature
- Desired or target pH
- Buffer toxicity (to the system)
- Buffer interactions with other system components
Boston Bioproducts Inc Phosphate Buffered Saline-Tween 10X, 4 x 1 L bottles, 0.5% Tween-20, pH 7.4, liquid
Phosphate Buffered Saline-Tween 10X, 4 x 1 L bottles, 0.5% Tween-20, pH 7.4, liquid
Rockland Immunochemicals 10X SDS-PAGE Running Gel Buffer (0.25 M Tris, 1.92 M Glycine, 1.0% SDS pH 8.3) - MB-017
SDS-PAGE, sodium dodecyl sulfate polyacrylamide gel electrophoresis, describes a technique widely used in biochemistry to separate proteins according to their electrophoretic mobility (a function of the length of a polypeptide chain and its charge) and no other physical feature. SDS is an anionic detergent applied to protein sample to linearize proteins and to impart a negative charge to linearized proteins. 10X SDS-PAGE Running Buffer is suitable for laboratory involved in protein biochemistry. Visit our newly expanded web site at www.rockland-inc.com for methods using this and other buffers.
Sigma Aldrich Fine Chemicals Biosciences Dulbeccos Modified Eagles500ML
Dulbeccos Modified Eagles500ML
Biotang Inc Tris-Glycine-SDS buffer (10X), 2000ml, Gel Running buffer
Tris-Glycine-SDS buffer (10X), 2000ml, Gel Running buffer.
Quality Biological Inc TBS 10X pH 7.4, 1000 mL
TBS 10X pH 7.4 is used as a washing buffer for cellular and molecular biology techniques. ¿ DNase, RNase and Protease tested. ¿ Applications: TBS 10X pH 7.4 will be useful as a washing buffer for electrodes, microtiter plates, and alkaline phophatase or peroxidase conjugates. TBS 10X pH 7.4 can also be used in detergent preparation and Western blot or ELISA applications. ¿ Storage Conditions: 15¿C to 30¿C ¿ Shipping Condition: ambient temperature ¿ Sterile Filtered ¿ DNase, RNase, and Protease Tested ¿ pH: 7.4¿0.1 ¿ Product and/or packaging contains recycled/renewable content ¿ Product and/or packaging is recyclable or reduces waste ¿ Product and/or packaging is safer to human and environmental health ¿ ¿ Max Shelf Life: 36 months
Quality Biological Inc DPBS without Calcium and Magnesium, 500 mL
DPBS is a balanced salt solution (BSS) ¿ Used for the handling and culturing of mammalian cells. ¿ Calcium and magnesium facilitate cell binding and clumping. ¿ DPBS without these ions can be used to wash and rinse suspended cells. ¿ Applications: DPBS serves as an irrigating, transporting or diluting fluid for mammalian cells, while maintaining cell tonicity and viability for a limited period of time. ¿ Used as a diluent of fresh red blood cells to study the trapping of red blood cell aggregate Liters ¿ Wash the wells of microtiter plates ¿ Wash cells during preparation and serial transfer. ¿ Storage Condition: 15¿C to 30¿C ¿ Shipping Condition: ambient temperature ¿ Sterile Filtered ¿ Product and/or packaging contains recycled/renewable content ¿ Product and/or packaging is recyclable or reduces waste ¿ Product and/or packaging is safer to human and environmental health ¿ UNSPSC: 12161700 ¿ ¿ Max Shelf Life: 24 months
Quality Biological Inc TBS HCl (10X), pH 7.2, 500 mL
Tris HCl, 10X pH 7.2 is a Buffered Saline Solution used in procedures for the isolation of DNA. ¿ Filtered through a 0.22 ¿m membrane ¿ DNase, RNase, and Protease tested ¿ Applications: Tris buffered saline is useful for the following: as a biological diluent buffer, as a buffer for antibodies, as a wash buffer for immunological assays including western blot, immunohistochemistry, immunofluorescence microscopy, and ELISA. ¿ Storage Conditions: 15¿C to 30¿C ¿ Shipping Condition: ambient temperature ¿ Sterile Filtered ¿ DNase, RNase and Protease Tested ¿ Product and/or packaging contains recycled/renewable content ¿ Product and/or packaging is recyclable or reduces waste ¿ Product and/or packaging is safer to human and environmental health ¿ ¿ Max Shelf Life: 36 months
Sigma Aldrich Fine Chemicals Biosciences MES Hydrate | 1266615-59-1 | MFCD00149409 | 1kg
MES Hydrate | Purity: 99% | Mol Wt: 195.24 (anhydrous basis) | 1266615-59-1 | MFCD00149409 | 1kg
Boston Bioproducts Inc Tris-Acetate Buffer (0.2 M, pH 7.0) - 500ml
The Tris-Acetate Buffer is a buffering agent composed of Tris-base and acetic acid. The combination of these ingredients yields a pH range of 6.0-8.0. This buffer is ideal for requiring a pH slightly above physiological conditions. Often used to support the electrophoresis of nucleic acids on agarose gel and protein electrophoresis employing SDS-PAGE and related blotting techniques.
Sigma Aldrich Fine Chemicals Biosciences MES hydrate >=99.5% (titration) | 1266615-59-1 | MFCD00149409 | 250G
MES hydrate >=99.5% (titration) | Purity: >=99.5% (titration) | 1266615-59-1 | MFCD00149409 | 250G
Boston Bioproducts Inc HBS-N Buffer (10X, pH 7.4) - 4x500ml
HBS-N Buffer (10X pH 7 4) - 4x500ml (For Research Use and Further Manufacturing Only)
Medchemexpress LLC PBS-T Powder | 100 POUCHES
MCE PBS-T Powder (1 L of 1x) is a formulation consisting of NaCl, KCl, Phosphate, and Tween 20. It is extensively utilized as a washing buffer in immunoassay experiments such as ELISA and Western Blot. Additionally, it can be employed for antibody dilution and the preparation of blocking solutions. The product is conveniently packaged, with 20 pouches defined as the base specification, and larger sizes offered as incremental volumes of this base. Each pouch is designed to be diluted to 1 L of 1x PBS-T buffer.
- Widely used in ELISA and Western Blot.
- Can be used for antibody dilution and preparation of blocking solution.
- Consists of NaCl, KCl, Phosphate, and Tween 20.
- Each pouch dilutes to 1 L of 1x PBS-T buffer.
- Eliminates the need to weigh and mix individual components.
- Convenient way to make PBS-T buffer.
- Final 1x PBS-T buffer has a pH of 7.4 ± 0.2 @ 25°C.
Rockland Immunochemicals 1.0 M Tris HCl pH 7.6 - MB-003
Tris solution is an abbreviation of the organic compound known as tris(hydroxymethyl)aminomethane. Tris is extensively used in biochemistry and molecular biology. In biochemistry, Tris is widely used as a component of buffer solutions, such as in TAE and TBE buffer, especially for solutions of nucleic acids.