Life Science Buffers
Balanced salt solutions and powders used for molecular, protein, nucleic acid, and cell biology applications including cell culture, electrophoresis, ELISA, and chromatography processes; available in a variety of formulations and grades.
Buffer solutions serve to keep pH (acidity or alkalinity) nearly constant in a variety of chemical and biological applications. For example, a bicarbonate buffer helps maintain the pH of blood, a buffered saline maintains cellular contents at a consistent pH level, and buffers help maintain a narrow pH range for enzymes to function correctly.
Most biological samples used in research are kept at a pH of about 7.4. Common biological buffers used for tissue culture include Dulbeccos phosphate buffered saline (PBS), Tris base, HEPES, MOPS, PIPES, and other formulations developed for specific cell lines or applications. Most buffers used in cell culture are also DNase-, RNase-, and protease-free.
The choice of buffer for a particular biological reaction or site depends on several factors:
- Temperature
- Desired or target pH
- Buffer toxicity (to the system)
- Buffer interactions with other system components
![Research Products International Corp MOPS [3-(N-morpholino)-propanesulfonic acid], 250 Grams](https://assets.fishersci.com/TFS-Assets/CCG/product-images/VN00006095-M92020-250.0.JPG-250.jpg)
Research Products International Corp MOPS [3-(N-morpholino)-propanesulfonic acid], 250 Grams
MOPS, or 3-(N-Morpholino) propanesulfonic acid, is a zwitterionic buffer. MOPS is primarily utilized as a buffering agent to maintain a stable pH in various experimental procedures. It is particularly effective in the pH range of 6.5 to 7.9, making it suitable for applications requiring slightly acidic to neutral pH conditions.
MOPS is commonly used in protein purification, enzyme assays, nucleic acid research, and electrophoresis techniques where a specific pH range needs to be maintained. Its buffering capacity and stability within the desired pH range make it valuable in these applications.
One of the significant advantages of MOPS is its compatibility with biological systems and enzymes. It exhibits minimal interference with enzymatic activity and other biological processes, ensuring reliable and accurate results.
pH Range: 6.5-7.9
![Research Products International Corp TBE Buffer [TRIS-Borate-EDTA], 10X Powder, 170.3 Grams of Powder Makes 1 Liter of Solution](https://assets.fishersci.com/TFS-Assets/CCG/product-images/VN00006095-T22020-1.0.JPG-250.jpg)
Research Products International Corp TBE Buffer [TRIS-Borate-EDTA], 10X Powder, 170.3 Grams of Powder Makes 1 Liter of Solution
Dry homogeneous blend of molecular biology grade TRIS base, Boric Acid and EDTA.
For use in gel electrophoresis.
Advanced Biomatrix Phosphate Buffered Saline 10X PBS 100 mL
Phosphate Buffered Saline (PBS) 10X is formulated and prepared to use in conjunction with other products provided by Advanced BioMatrix such as Type I collagen for forming a 3D hydrogel. PBS 10X is provided in a user-friendly packaging for use and storage. This product is sterile and supplied as a ready to use solution.
Teknova 1M Tris-HCl, pH 7.4. 1000 mL, Sterile.
Tris (tris(hydroxymethyl)aminomethane) is used as a component ofbuffer solutions, especially for solutions ofnucleic acids. The useful buffer range for tris (pH 7–9) is similar to the pH of most living organisms. This, and its low cost, make tris one of the most common buffers in the biology/biochemistry laboratory.
Bioworld Bis-Tris, 100 g
Synonym: 2,2-Bis(hydroxymethyl)-2,2′,2″-nitrilotriethanol, 2-Bis(2-hydroxyethyl)amino-2-(hydroxymethyl)-1,3-propanediol, Bis(2-hydroxyethyl)amino-tris(hydroxymethyl)methane A common biological bufferAn inert, zwitterionic buffer that exhibits only a very small shift in dissociation constantSpecifications:Ultra Pure GradepKa (25°C): 6.5+/-0.2Appearance: SolidBoiling Point : 466.18 °C at 760 mmHg (Predicted)Density : 1.35 g/cm3 (Predicted)Melting Point: 102-105 °CPH Value: 5.8-7.2
![Research Products International Corp MOPS, Sodium Salt [3-(N-Morpholino)-propanesulfonic acid sodium salt], 1 Kilogram](https://assets.fishersci.com/TFS-Assets/CCG/product-images/VN00006095-M92040-1000.0.JPG-250.jpg)
Research Products International Corp MOPS, Sodium Salt [3-(N-Morpholino)-propanesulfonic acid sodium salt], 1 Kilogram
MOPS Sodium Salt, also known as 3-(N-Morpholino) propanesulfonic acid sodium salt, is a chemical compound that plays a significant role in the life science marketplace. It is a zwitterionic buffer commonly used in biological and biochemical research and is primarily utilized as a buffering agent to maintain a stable pH in various experimental procedures.
It is particularly effective in the pH range of 6.5 to 7.9, making it suitable for applications requiring slightly acidic to neutral pH conditions. MOPS Sodium Salt is commonly used in protein purification, enzyme assays, nucleic acid research, and electrophoresis techniques where a specific pH range needs to be maintained. Its buffering capacity and stability within the desired pH range make it valuable in these applications.
Alkali Scientific CellPro™ Phosphate Buffered Saline 10X, with Ca and Mg, pH 7.2-7.4, 500mL
Alkali Scientific™ CellPro™ PBS (Phosphate Buffered Saline) provides researchers with a reliable and consistent solution essential for various molecular and cellular biology applications. This meticulously prepared buffer is formulated to maintain a stable pH and osmolarity, ensuring optimal conditions for cell culture, protein assays, immunohistochemistry, and many other biological techniques. With its phosphate-based formulation, CellPro™ PBS offers excellent buffering capacity, making it suitable for maintaining the physiological pH of biological samples and preserving their integrity. Whether used for cell washing, sample dilution, or buffer exchange, CellPro™ PBS guarantees exceptional performance and reproducibility in every experiment. Trust in the quality and reliability of CellPro™ PBS to support your research needs and enable precise and consistent results.
Alkali Scientific QuickSilver PBS-T Buffer Powder, 100 Pouches
QuickSilver™ Buffer Powder packs offer extreme convenience and will change the way you prepare buffers in your lab. They are quick, and easy to use. Convenient premeasured pouches make 1L of 1X buffer. There is no pH adjusent needed and they offer a long shelf life. They come supplied with preprinterd bottle labels for easy identification. 100 pouches per pack.
Alkali Scientific CellPro™ TAE buffer (Tris-acetate EDTA), 10X, 500mL
Alkali Scientific™ CellPro™ TAE Tris Acetate EDTA is a fundamental reagent in nucleic acid electrophoresis, renowned for its exceptional buffering capacity and DNA solubilization properties. Designed to provide optimal performance and reproducibility, this solution enables precise separation and analysis of DNA fragments in agarose gel electrophoresis. Each batch undergoes rigorous sterile filtration to ensure purity and consistency, empowering researchers with confidence in their molecular biology experiments.
Alkali Scientific CellPro™ Tris-HCL, 1M Stock Solution, pH 7.2, 1L
Alkali Scientific™ CellPro™ Tris solution offers sterile and filtered Tris buffer for various laboratory applications. This high-quality solution is meticulously prepared to ensure purity and consistency, meeting the stringent requirements of scientific research and experimentation. With its sterile formulation, it minimizes the risk of contamination, making it suitable for a wide range of cell culture and molecular biology procedures. The filtered nature of the solution ensures clarity and reliability in experimental results, providing researchers with confidence in their findings. Whether used in protein biochemistry, DNA electrophoresis, or other molecular biology techniques, CellPro™ Tris solution delivers reliable performance and consistent results, contributing to the success of laboratory workflows.
Cell Signaling Technology Tris-Glycine SDS Running Buffer (10X) 1000 ml
Tris-Glycine SDS Running Buffer (10X) 1000 ml
![Research Products International Corp MOPS [3-(N-morpholino)-propanesulfonic acid], 5 Kilograms](https://assets.fishersci.com/TFS-Assets/CCG/product-images/VN00006095-M92020-5000.0.JPG-250.jpg)
Research Products International Corp MOPS [3-(N-morpholino)-propanesulfonic acid], 5 Kilograms
MOPS, or 3-(N-Morpholino) propanesulfonic acid, is a zwitterionic buffer. MOPS is primarily utilized as a buffering agent to maintain a stable pH in various experimental procedures. It is particularly effective in the pH range of 6.5 to 7.9, making it suitable for applications requiring slightly acidic to neutral pH conditions.
MOPS is commonly used in protein purification, enzyme assays, nucleic acid research, and electrophoresis techniques where a specific pH range needs to be maintained. Its buffering capacity and stability within the desired pH range make it valuable in these applications.
One of the significant advantages of MOPS is its compatibility with biological systems and enzymes. It exhibits minimal interference with enzymatic activity and other biological processes, ensuring reliable and accurate results.
pH Range: 6.5-7.9
Research Products International Corp PIPES FREE ACID ULTRAPURE, 1 KILOGRAM
Heavy metals: <10 ppm
pka (25°C): 6.8
The working pH range in aqueous solution is 6.5-7.5