Life Science Buffers
Balanced salt solutions and powders used for molecular, protein, nucleic acid, and cell biology applications including cell culture, electrophoresis, ELISA, and chromatography processes; available in a variety of formulations and grades.
Buffer solutions serve to keep pH (acidity or alkalinity) nearly constant in a variety of chemical and biological applications. For example, a bicarbonate buffer helps maintain the pH of blood, a buffered saline maintains cellular contents at a consistent pH level, and buffers help maintain a narrow pH range for enzymes to function correctly.
Most biological samples used in research are kept at a pH of about 7.4. Common biological buffers used for tissue culture include Dulbeccos phosphate buffered saline (PBS), Tris base, HEPES, MOPS, PIPES, and other formulations developed for specific cell lines or applications. Most buffers used in cell culture are also DNase-, RNase-, and protease-free.
The choice of buffer for a particular biological reaction or site depends on several factors:
- Temperature
- Desired or target pH
- Buffer toxicity (to the system)
- Buffer interactions with other system components
eMolecules tert-Butyl 4-(hydroxymethyl)benzoate | 143726-85-6 | MFCD04973449 | 1g
Ambeed | tert-Butyl 4-(hydroxymethyl)benzoate | 1g | 592282779 | A321260 | | 143726-85-6 | MFCD04973449 | 208.257 | C12H16O3
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Medchemexpress LLC MOPSO sodium | 79803-73-9 | 97.0% | 247.24 | C7H14NNaO5S | 500 G
MOPSO sodium is the sodium salt of MOPSO, a zwitterionic buffering agent used in biochemical and molecular biology applications to prepare buffer solutions.
- Used for preparation of buffer solutions.
- Zwitterionic buffering agent; effective pH range typically near physiological pH.
- CAS number 79803-73-9.
- Chemical formula C7H14NNaO5S; molecular weight 247.24.
- Purity approximately 97.0% and available in 500 g packages.
Boston Bioproducts Inc Phosphate Buffered Saline, PBS (10X, for Western Blot Washing) - 1L
Phosphate Buffered Saline PBS (10X for Western Blot Washing) - 1L (For Research Use and Further Manufacturing Only)
Boston Bioproducts Inc HBS-EP+ Buffer (10X, pH 7.4) - 4x500ml
HBS-EP Plus Buffer (10X pH 7 4) - 4x500ml (For Research Use and Further Manufacturing Only)
Enterprise Technology Solutions Expanding File Jan-Dec, 9" Expansion, 12 Sections, 1/12-Cut Tab, Letter Size, Brown, Elastic Cord
ABILITYONE 7530016819290 Skilcraft Increase your efficiency with this letter-size expanding file featuring 12 tabs for monthly filing. The accordion-style pocket folder is covered with soil-resitant leather-patterned paper and is durable to stand up to heavy handling.
![Research Products International Corp RPI PIPES [Piperazine-N-N'-bis(2-ethanesulfonic acid)], 5 Kilograms](https://assets.fishersci.com/TFS-Assets/CCG/product-images/VN00006095-P40140-5000.0.JPG-250.jpg)
Research Products International Corp RPI PIPES [Piperazine-N-N'-bis(2-ethanesulfonic acid)], 5 Kilograms
Heavy metals: <10 ppm
pka (25°C): 6.8
The working pH range in aqueous solution is 6.5-7.5
Chem-Impex International, Inc. 2-(N-Cyclohexylamino)ethanesulfonic acid | 103-47-9 | MFCD00003835 | 100G
2-(N-Cyclohexylamino)ethanesulfonic acid, 103-47-9, MFCD00003835, 100G
LAB BIOREAGENTS LowBinding V Buffer 500ml
Lab Bioreagents LowBindings (I, II, III, IV, V, VI, VII) are newly developed assay buffers that provides exceptional performance and broad compatibility for Western blot, ELISA, IHC and nucleic acid detection assays. The LowBindings helps to reduce the non-specific bindings, negative effects of interfering molecules, and cross-reactivities of antibodies and proteins from biomatrix. Therefore, LowBindings exhibit the low matrix interference and high signal-to-noise, by which the assay sensitivity and specificity have been significantly improved. As many factors are involved in immunoassays, there is no single assay buffer that is ideal for each assay. LowBindings (I, II, III, IV, V, VI, VII) are a variety of buffers based on different formulations.
Medchemexpress LLC N,N-Bis(2-hydroxyethyl)-2-aminoethanesulfonic acid | 10191-18-1 | 98.0% | 500 G
BES is a secondary standard biochemical buffer used in diagnostic testing. It offers a stable pH environment for various applications.
- Effective pH range of 6.4 to 7.8
- pKa of 7.15 at 20 °C
- Suitable for diagnostic testing areas
Medchemexpress LLC HEPPS | 16052-06-5 | 99.0% | 50 G
HEPPS (EPPS) is a buffering agent with a useful pH range from 7.3 to 8.7. It reduces Aβ-aggregate-induced memory deficits and rescues cognitive deficits in mice. EPPS is orally active and penetrates the blood-brain barrier. It binds to amyloid-β aggregates and converts them into monomers, thereby reducing the formation of amyloid-β oligomers and plaques. This agent also rescues cell damage induced by amyloid-β aggregates.
- Binds to amyloid-β aggregates and converts them into monomers
- Reduces formation of amyloid-β oligomers and plaques
- Rescues cell damage induced by amyloid-β aggregates
- Improves cognitive dysfunction in APP/PS1 transgenic mice
- Reduces Aβ oligomer and plaque deposition in the brain
- Reduces glial GABA release in the brain
Thermo Scientific Production Chemicals and Services DOE AND INGALLS
NC3959187 ELUTE BUFFER A 50MM TRIS HCL8
Thermo Scientific Production Chemicals and Services DOE AND INGALLS
NC3959188 ELUTE BUFFER B 50MM TRIS HCL8