Life Science Buffers
Balanced salt solutions and powders used for molecular, protein, nucleic acid, and cell biology applications including cell culture, electrophoresis, ELISA, and chromatography processes; available in a variety of formulations and grades.
Buffer solutions serve to keep pH (acidity or alkalinity) nearly constant in a variety of chemical and biological applications. For example, a bicarbonate buffer helps maintain the pH of blood, a buffered saline maintains cellular contents at a consistent pH level, and buffers help maintain a narrow pH range for enzymes to function correctly.
Most biological samples used in research are kept at a pH of about 7.4. Common biological buffers used for tissue culture include Dulbeccos phosphate buffered saline (PBS), Tris base, HEPES, MOPS, PIPES, and other formulations developed for specific cell lines or applications. Most buffers used in cell culture are also DNase-, RNase-, and protease-free.
The choice of buffer for a particular biological reaction or site depends on several factors:
- Temperature
- Desired or target pH
- Buffer toxicity (to the system)
- Buffer interactions with other system components
Gold Biotechnology Inc Tris HCl 500 g
Tris hydrochloride is a reagent that is used for the stabilization of buffer formulations and electrophoresis of biological molecules
Bioworld BES-Buffered Saline (2X), 100 mL
Product DescriptionBES (N,N-bis(2-hydroxyethyl)-2-aminoethanesulfonic acid) saline buffer has a concentration of 2X and a pH of 7.1 at 25°C. BES is a zwitterionic buffer used in biochemistry and molecular biology, and has been used to study versatile catalyst precursors for mild hydrocarboxylation of alkanes to carboxylic acids. BES was used to determine that saline buffers minimize the action of interfering factors in the bacterial endotoxins test. This saline buffer contains 50 mM BES, 1.5 mM Na2HPO4, and 280 mM NaCl.For laboratory use only. Not fit for agricultural, household, clinical use or human consumption.
Gold Biotechnology Inc MES Free Acid 250 g
MES is a buffering agent used in biochemistry and molecular biology that was selected and described by Good et al It is a zwitterionic morpholinic buffer that is useful for a pH range of 5 5 6 7 and commonly used for cell culture media as a running buffer in electrophoresis and for protein purification in chromatography MES lacks the ability to form a complex with most metal ions and is recommended for use as a non-coordinating buffer in solutions with metal ions MES is often used in buffered culture media for bacteria yeast and mammalian cells It is toxic towards plants at high concentrations but can be used in culture media at lower concentrations for plants Due to low ionic mobility MES is regarded as an excellent buffer for use in capillary electrochromatography MES is often employed as a substitute for the highly toxic cacodylate as well as a substitute for the non-zwitterionic buffers citrate and malate
Teknova 1M Tris-HCl, pH 8.0 Solution, 1000mL, Sterile. 2 Pack.
Tris (tris(hydroxymethyl)aminomethane) is used as a component ofbuffer solutions, especially for solutions ofnucleic acids. The useful buffer range for tris (pH 7–9) is similar to the pH of most living organisms. This, and its low cost, make tris one of the most common buffers in the biology/biochemistry laboratory.
Chrono Log Corporation Saline (0.9%), 15mL
This item has a minimum qty of 4 per supplier requirements.
Sterile physiological saline for irrigation (0.85% or 0.9% w/v) for CHRONOPAR Reagent preparation and for dilution of the Whole Blood specimen.
![Research Products International Corp TAPS, [Tris(hydroxymethyl)-3-aminopropanesulfonic acid], Free Acid, 500 Grams](https://assets.fishersci.com/TFS-Assets/CCG/product-images/VN00006095-T12000-500.0.JPG-250.jpg)
Research Products International Corp TAPS, [Tris(hydroxymethyl)-3-aminopropanesulfonic acid], Free Acid, 500 Grams
pKa(25°:C): 8.4. The working pH range in aqueous solution is 7.7-9.1.
TARGETMOL CHEMICALS INC P62-mediated mitophagy inducer
Also available in 1 mL, 2 mg, 5 mg, 10 mg, 50 mg, 100 mg, 200 mg and bulk. Please contact Fisher for quotes. P62-mediated mitophagy inducer (PMI) is a regulator of mitophagy. Purity 99.35%
Fd Neurotechnologies Inc PHOSPHATE-BUFFERED 4% PARAFORM
This 1000ml filtered and ready-to-use tissue fixative is freshly made from high purity paraformaldehyde powder. This solution may be stored at room temperature for at least one year without change of the original quality. The standard solution contains 4% paraformaldehyde in 0.1 M phosphate buffer (pH 7.4). Other concentrations can also be provided upon request.
Medchemexpress LLC MES potassium (2-Morpholinoethanesulphonic acid potassium) | 39946-25-3 | 99.88% | 5 G
MES potassium (2-Morpholinoethanesulphonic acid potassium) is an amphoteric ion buffer with a buffer capacity ranging from pH 5.5-7.0. It is a Good's buffer widely used in biochemistry and molecular biology experiments, including cell culture, enzyme activity determination, electrophoresis, and protein studies.
- Amphoteric ion buffer with pH range 5.5-7.0.
- Provides effective buffering capacity around pH 6.15.
- pKa value changes with temperature, requiring consideration at varying temperatures.
- Suitable for cell culture, enzyme activity determination, electrophoresis, and protein studies.
- Exhibits poor affinity with metal ions, making it suitable for studies involving such ions.
Rockland Immunochemicals 1X TE pH 8.0 (10 mM Tris HCl, 1.0 mM EDTA) DEPC TREATED - MB-040
TE buffer is a commonly used buffer solution in molecular biology, especially in procedures involving DNA or RNA. Tris-EDTA buffer is derived from its components: Tris, a common pH buffer, and EDTA, a molecule that chelates cations like Mg2+. The purpose of TE buffer is to solubilize DNA or RNA, while protecting it from degradation.