Life Science Buffers
Balanced salt solutions and powders used for molecular, protein, nucleic acid, and cell biology applications including cell culture, electrophoresis, ELISA, and chromatography processes; available in a variety of formulations and grades.
Buffer solutions serve to keep pH (acidity or alkalinity) nearly constant in a variety of chemical and biological applications. For example, a bicarbonate buffer helps maintain the pH of blood, a buffered saline maintains cellular contents at a consistent pH level, and buffers help maintain a narrow pH range for enzymes to function correctly.
Most biological samples used in research are kept at a pH of about 7.4. Common biological buffers used for tissue culture include Dulbeccos phosphate buffered saline (PBS), Tris base, HEPES, MOPS, PIPES, and other formulations developed for specific cell lines or applications. Most buffers used in cell culture are also DNase-, RNase-, and protease-free.
The choice of buffer for a particular biological reaction or site depends on several factors:
- Temperature
- Desired or target pH
- Buffer toxicity (to the system)
- Buffer interactions with other system components
Sigma Aldrich Fine Chemicals Biosciences HEPES BioPerformance Certified, >=99.5% (titration), suitable for cell culture | 7365-45-9 | MFCD00006158 | 100G
HEPES BioPerformance Certified, >=99.5% (titration), suitable for cell culture | Purity: >=99.5% (titration) | Mol Wt: 238.3 | 7365-45-9 | MFCD00006158 | 100G
Boston Bioproducts Inc Phosphate Buffered Saline, PBS (pH 7.4, Sterile-Filtered) - 1L
Phosphate Buffered Saline (PBS) is a versatile buffer solution widely used in biological research due to its isotonic and non-toxic nature to cells. With a pH range of 7.0 to 7.4, PBS mimics the osmolarity and ion concentrations of the human body, making it ideal for a variety of applications including protein purification, as wash buffer in Western blot and immunoassays, sample dilution, and as a transport medium for tissue samples as well as RNA viruses.
STEMCELL Technologies EasySep™ Red Blood Cell Lysis Buffer, Size: 100 mL
EasySep™ Red Blood Cell Lysis Buffer is for use as directed with EasySep™ or RoboSep™ Whole Blood kits for the lysis of red blood cells prior to separation from whole blood.
Teknova 1X PBS Solution, pH 7.4. 1000mL, Sterile
Phosphate-buffered saline (PBS) is a salt solution that is commonly used in physiological and biochemical experiments. It can be used to maintain pH neutrality or to buffer against changes in pH in order to provide optimal conditions for cellular growth and function. The buffering range is between pH 6.8 and 7.4.
Hampton Research HR2-902-01/StockOptions HEPES #01 pH 6.8, 185 ml
HR2-902-01/StockOptions HEPES #01 pH 6.8, 185 ml. Please note items from this supplier are non-returnable.
Teknova 1M Tris HCL, pH 7.5, 500mL, Rnase/Endo Tested, Sterile. 8 pack.
1M Tris HCL, pH 7.5, 500mL, Rnase/Endo Tested, Sterile. 8 pack.
Apexbio Technology LLC PBS Liposomes(Synonyms: Phosphate Buffered Saline Liposomes, Empty Liposomes, Blank Liposomes), 10mL.
PBS Liposomes consist of phosphate-buffered saline (PBS) encapsulated within lipid bilayers serving primarily as control reagents in macrophage depletion studies involving clodronate liposomes Unlike clodronate-loaded liposomes PBS liposomes lack active agents targeting macrophages and thus do not induce macrophage apoptosis They function to discriminate between the biological effects mediated by liposomal delivery systems versus those specifically attributable to encapsulated clodronate PBS Liposomes are commonly employed in comparative experimental designs to assess specific macrophage depletion outcomes
Teknova 2X HBS (HEPES-Buffered Saline), pH 7.05. 250mL, Endotoxin Tested. Sterile. 4 pack.
HEPES solution is a Goods buffer that is commonly used in cell culture and biochemical applications. It is well known for its ability to maintain pH level, making it an essential part of any experiment that requires a stable pH. It has a buffering range of 6.8 - 8.2.
Teknova 1M HEPES, pH 7.5, 1000mL Sterile. 2 Pack.
HEPES solution is a Goods buffer that is commonly used in cell culture and biochemical applications. It is well known for its ability to maintain pH level, making it an essential part of any experiment that requires a stable pH. It has a buffering range of 6.8 - 8.2.