Life Science Buffers
Balanced salt solutions and powders used for molecular, protein, nucleic acid, and cell biology applications including cell culture, electrophoresis, ELISA, and chromatography processes; available in a variety of formulations and grades.
Buffer solutions serve to keep pH (acidity or alkalinity) nearly constant in a variety of chemical and biological applications. For example, a bicarbonate buffer helps maintain the pH of blood, a buffered saline maintains cellular contents at a consistent pH level, and buffers help maintain a narrow pH range for enzymes to function correctly.
Most biological samples used in research are kept at a pH of about 7.4. Common biological buffers used for tissue culture include Dulbeccos phosphate buffered saline (PBS), Tris base, HEPES, MOPS, PIPES, and other formulations developed for specific cell lines or applications. Most buffers used in cell culture are also DNase-, RNase-, and protease-free.
The choice of buffer for a particular biological reaction or site depends on several factors:
- Temperature
- Desired or target pH
- Buffer toxicity (to the system)
- Buffer interactions with other system components
![eMolecules 220598-43-6 | tert-butyl 4-(hydroxymethyl)-2-azabicyclo[2.1.1]hexane-2-carboxylate | MFCD18073347 | 1g](https://assets.fishersci.com/TFS-Assets/CCG/product-images/502159302.JPG-250.jpg)
eMolecules 220598-43-6 | tert-butyl 4-(hydroxymethyl)-2-azabicyclo[2.1.1]hexane-2-carboxylate | MFCD18073347 | 1g
Ambeed | (4-((4-(tert-Butoxycarbonyl)piperazin-1-yl)sulfonyl)phenyl)boronic acid | 250mg | 731676797 | A260715 | 486422-54-2 | MFCD28384269 | 370.230 | C15H23BN2O6S
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![Medchemexpress LLC 2-[1,4,7-triazacyclononan-1-yl-4,7-bis(tBu-ester)]-1,5-pentanedioic acid | 1190101-34-8 | MFCD29083820 | 99.8% | 543.69 g/mol | C27H49N3O8 | 25 MG](https://assets.fishersci.com/TFS-Assets/CCG/product-images/5000284202.png-250.jpg)
Medchemexpress LLC 2-[1,4,7-triazacyclononan-1-yl-4,7-bis(tBu-ester)]-1,5-pentanedioic acid | 1190101-34-8 | MFCD29083820 | 99.8% | 543.69 g/mol | C27H49N3O8 | 25 MG
NODAGA-tris(t-Bu ester) (NODAG) is a bifunctional chelator designed to conjugate peptides, antibodies, and other biomolecules for subsequent radiolabeling in PET imaging. Provided as a solid reagent for research use, it combines high purity with practical solubility and storage properties for laboratory workflows.
- High purity suitable for conjugation and radiolabeling.
- Off-white to light yellow solid for easy handling.
- Soluble in DMSO at 100 mg/mL with ultrasonic assistance.
- Example in vivo formulations achieve ≥2.5 mg/mL clear solutions.
- Stable under recommended storage: powder at -20°C (3 years) or 4°C (2 years).
- Suitable for labeling peptides and antibodies for PET imaging.
Bioworld TT Buffer, Ready-Pack (10X), 1 L
Tris-Tricine BufferTris-Tricine (TT) Buffer has been developed for both non-denaturing and denaturing electrophoresis of proteins, and offers several advantages over more conventionally used buffers. TT Buffer allows for optimal resolution of low molecular weight proteins and elimantes the glycine front often found in gel utilizing TG-SDS and other such beffers. This improved running buffer allows for resolution of peptides as low as 5kD. TT buffer is also excellent for protein electrophoresis where resolved products will be transferred to a nylon membrane. Unlike glycine, tricine will not interfere with membrane protein sequencing. The 10X pack just requires the addition of distilled H2O to make 1.0 Liter of 10X liquid. A simple 10-fold dilution with water provides a 1X solution, that makes an excellent running buffer for most applications. Technical Information:Ultra PureGradeStorage: RTHazard: Irritant
Kd Medical Inc TRIS GLYCIN TRANS BUF 10X, 1L
Tris-Glycine Transfer Buffer (10X) is used as a transfer buffer during western blotting applications. 10X Tris Glycine is concentrated and ready to be diluted to a 1X working solution. One 1 liter bottle.
Kd Medical Inc TRIS BUFF SALINE 10X,PH7.4,1L
Tris Buffered Saline 10X is ready to use for dilution to a 1X working solution (pH 7.4). Tris Buffered Saline (TBS) is a pH stabilizing solution used for western blot and ELISA procedures. One 1 liter bottle.
eMolecules 1179360-20-3 | TERT-BUTYL 3-(HYDROXYMETHYL)-1,4-DIAZEPANE-1-CARBOXYLATE HCL | MFCD23702017 | 1g
Ambeed | (R)-Oxiran-2-ylmethyl 4-nitrobenzenesulfonate | 1g | 628567785 | A635502 | 123750-60-7 | MFCD08460169 | 259.230 | C9H9NO6S
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![eMolecules benzyl N-[[4-(hydroxymethyl)-2-oxabicyclo[2.2.2]octan-1-yl]methyl]carbamate | 1g](https://assets.fishersci.com/TFS-Assets/CCG/product-images/502160023.JPG-250.jpg)
eMolecules benzyl N-[[4-(hydroxymethyl)-2-oxabicyclo[2.2.2]octan-1-yl]methyl]carbamate | 1g
Pharmablock | benzyl N-[[4-(hydroxymethyl)-2-oxabicyclo[2.2.2]octan-1-yl]methyl]carbamate | 1g | 551109237 | PBJP030 | 305.374 | C17H23NO4
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Bioworld Tris/Glycine/SDS Buffer for Resolve Gel (1X), 40 PK(s)
1X Tris/Glycine/SDS Buffer for Resolve GelBuffer System: Pre-weighed Powder mix in pouches(packs)1 pouch(pack)= 500 mL