Life Science Buffers
Balanced salt solutions and powders used for molecular, protein, nucleic acid, and cell biology applications including cell culture, electrophoresis, ELISA, and chromatography processes; available in a variety of formulations and grades.
Buffer solutions serve to keep pH (acidity or alkalinity) nearly constant in a variety of chemical and biological applications. For example, a bicarbonate buffer helps maintain the pH of blood, a buffered saline maintains cellular contents at a consistent pH level, and buffers help maintain a narrow pH range for enzymes to function correctly.
Most biological samples used in research are kept at a pH of about 7.4. Common biological buffers used for tissue culture include Dulbeccos phosphate buffered saline (PBS), Tris base, HEPES, MOPS, PIPES, and other formulations developed for specific cell lines or applications. Most buffers used in cell culture are also DNase-, RNase-, and protease-free.
The choice of buffer for a particular biological reaction or site depends on several factors:
- Temperature
- Desired or target pH
- Buffer toxicity (to the system)
- Buffer interactions with other system components
Boston Bioproducts Inc Phosphate Buffered Saline, PBS (10X without K, pH 7.4) - 1L
Phosphate Buffered Saline PBS (10X without K pH 7 4) - 1L (For Research Use and Further Manufacturing Only)
Boston Bioproducts Inc Tris-HCl Buffer (1 M, pH 7.4, RNase Free) - 250ml
Tris-HCl Buffer (1 M pH 7 4 RNase Free) - 250ml (For Research Use and Further Manufacturing Only)
Boston Bioproducts Inc M9 Minimal Salts Solution - 2x1L
M9 Minimal Salts Solution - 2x1L (For Research Use and Further Manufacturing Only)
Boston Bioproducts Inc PIPES Buffer (1 M, pH 7.0) - 500ml
PIPES Buffer (1 M pH 7 0) - 500ml (For Research Use and Further Manufacturing Only)
Biotang Inc TRIS-HCL Buffer 1.0M, pH 7.6 (0.2 micron filtered), 500 ml
TRIS-HCL Buffer 1.0M, pH 7.6 (0.2 micron filtered), 500 ml.
Boston Bioproducts Inc Tris-HCl Buffer (1 M, pH 7.8) - 1L
Tris-HCl Buffer (1 M pH 7 8) - 1L (For Research Use and Further Manufacturing Only)
Boston Bioproducts Inc HEPES Buffered Saline (5X, pH 7.4) - 500ml
HEPES Buffered Saline (5X pH 7 4) - 500ml (For Research Use and Further Manufacturing Only)
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eMolecules 1036262-54-0 | trans-2,4-Bis-hydroxymethyl-azetidine-1-carboxylic acid tert-butyl ester | J & W PharmLab LLC434.530 | C20H38N2O8 | 96.000 | CC(C)(C)OC(=O)N1[C@@H](CO)C[C@@H]1CO.CC(C)(C)OC(=O)N1[C@H](CO)C[C@H]1CO | 1g | 534135067
trans-2,4-Bis-hydroxymethyl-azetidine-1-carboxylic acid tert-butyl ester | J & W PharmLab LLC | 1036262-54-0434.530 | C20H38N2O8 | 96.000 | CC(C)(C)OC(=O)N1[C@@H](CO)C[C@@H]1CO.CC(C)(C)OC(=O)N1[C@H](CO)C[C@H]1CO | 1g | 534135067
Sigma Aldrich Fine Chemicals Biosciences Tris(hydroxymethyl)aminomethane ACS reagent, >=99.8% | 77-86-1 | MFCD00004679 | 100G
Tris(hydroxymethyl)aminomethane ACS reagent, >=99.8% | Purity: >=99.8%; 99.8-100.1% | Mol Wt: 121.14 | 77-86-1 | MFCD00004679 | 100G
Boston Bioproducts Inc Phosphate Buffered Saline-Tween, PBST (20X, with 1% Tween-20, pH 7.4) - 4x1L
Phosphate Buffered Saline-Tween PBST (20X with 1% Tween-20 pH 7 4) - 4x1L (For Research Use and Further Manufacturing Only)
Boston Bioproducts Inc HEPES Buffer (1 M, pH 8.5) - 500ml
HEPES Buffer (1 M pH 8 5) - 500ml (For Research Use and Further Manufacturing Only)
Sigma Aldrich Fine Chemicals Biosciences HEPES sodium salt solutin500ML
HEPES is a general-purpose zwitterionic buffer which does not bind magnesium calcium manganese(II) or copper(II) ions. HEPES has been classified as Good buffer by Dr. Norman Good and his colleagues in 1966. It is zwitterionic at biological pH and is most effective in the pH range of pH 6.8 to 8.2.