Life Science Buffers
Balanced salt solutions and powders used for molecular, protein, nucleic acid, and cell biology applications including cell culture, electrophoresis, ELISA, and chromatography processes; available in a variety of formulations and grades.
Buffer solutions serve to keep pH (acidity or alkalinity) nearly constant in a variety of chemical and biological applications. For example, a bicarbonate buffer helps maintain the pH of blood, a buffered saline maintains cellular contents at a consistent pH level, and buffers help maintain a narrow pH range for enzymes to function correctly.
Most biological samples used in research are kept at a pH of about 7.4. Common biological buffers used for tissue culture include Dulbeccos phosphate buffered saline (PBS), Tris base, HEPES, MOPS, PIPES, and other formulations developed for specific cell lines or applications. Most buffers used in cell culture are also DNase-, RNase-, and protease-free.
The choice of buffer for a particular biological reaction or site depends on several factors:
- Temperature
- Desired or target pH
- Buffer toxicity (to the system)
- Buffer interactions with other system components
Indofine Chemical 1,3,5-Triphenyl Tetraz, 1 Gm
1,3,5-TRIPHENYL TETRAZOLIUM FORMAZAN, Products for Life Science, 531-52-2, 1 gm
![eMolecules 13113-08-1 | N-(3-Indolylacetyl)glycine | Toronto Research Chemicals | MFCD00079392 | 232.239 | C12H12N2O3OC(=O)CNC(=O)Cc1c[nH]c2ccccc12 | 250mg | 601597193](https://assets.fishersci.com/TFS-Assets/CCG/product-images/502174712.JPG-250.jpg)
eMolecules 13113-08-1 | N-(3-Indolylacetyl)glycine | Toronto Research Chemicals | MFCD00079392 | 232.239 | C12H12N2O3OC(=O)CNC(=O)Cc1c[nH]c2ccccc12 | 250mg | 601597193
N-(3-Indolylacetyl)glycine | Toronto Research Chemicals | 13113-08-1 | MFCD00079392 | 232.239 | C12H12N2O3OC(=O)CNC(=O)Cc1c[nH]c2ccccc12 | 250mg | 601597193
Bioworld ADA Buffer, 0.2M, pH 6.0, 500 mL
Product DescriptionADA (N-(2-acetamido)iminodiacetic acid) buffer has a concentration of 0.2M and a pH value of 6.0. It has a molecular weight of 190.2 and formula of C6H10N2O5. ADA is a zwitterionic buffer used in biochemistry and molecular biology, such as its use in protein-free medium for supporting chick embryo fibroblasts. It has also been shown to interfere with color development in bicinchoninic acid assay (BCA) for protein quantitation and used in isoelectric focusing of proteins in immobilized pH gradients.For laboratory use only. Not fit for agricultural, household, clinical use or human consumption.
Medchemexpress LLC 20 mM HEPES, 150 mM NaCl, 240 mM sucrose, pH 7.2 buffer | 30 ML
This physiological buffer is composed of 20 mM HEPES, 150 mM NaCl, and 240 mM Sucrose, adjusted to pH 7.2. It is primarily utilized for resuspending human erythrocyte ghost preparations and acts as the final resuspension medium for purified human right-side-out and inside-out erythrocyte ghosts.
- Composed of 20 mM HEPES, 150 mM NaCl, and 240 mM sucrose
- Adjusted to pH 7.2
- Used for resuspending human erythrocyte ghost preparations
- Serves as final resuspension medium for purified human right-side-out and inside-out erythrocyte ghosts
- Liquid appearance
- Colorless to light yellow
Medchemexpress LLC Ada disodium | 41689-31-0 | 98.0% | 10 G
ADA (disodium) is a buffer salt derived from ADA (adenosine deaminase) and is widely used in biochemical research, especially in the preparation of enzyme buffers. It is for research use only.
- Buffer salt derived from adenosine deaminase
- Widely used in biochemical research
- Used in the preparation of enzyme buffers
Apexbio Technology LLC HEPES Sodium salt 75277-39-3 250g
HEPES Sodium salt (CAS 75277-39-3) is a zwitterionic buffering agent designed to stabilize hydrogen ion concentration and maintain physiological pH (6 8 8 2) in biological systems thereby supporting cellular function and biomolecular interactions HEPES Sodium salt exerts its biological activity primarily through non-volatile amphoteric chemical buffering In in vitro and cell-based settings HEPES Sodium salt supports cell viability and experimental consistency by minimizing pH fluctuations Based on these properties HEPES Sodium salt holds research potential in cell-based assays maintenance of cultured mammalian cells controlled in vitro experiments studies of lysosomal biogenesis and investigations of cellular metabolic mechanisms
Chem-Impex International, Inc. Piperazine-N,N'-bis(2-ethanesulfonic acid) | 5625-37-6 | MFCD00006159 | 100G
Piperazine-N,N'-bis(2-ethanesulfonic acid), 5625-37-6, MFCD00006159, 100G
Medchemexpress LLC PIPES dipotassium | 108321-27-3 | MFCD00069751 | 98.0% | 378.55 | C8H16K2N2O6S2 | 10 G
PIPES dipotassium is a zwitterionic buffering agent used in biochemical and biological assays to maintain a stable, near-neutral pH. The dipotassium salt provides good aqueous solubility and is compatible with many enzymatic and cell-based applications.
- Maintains pH in the 6.1-7.5 buffering range.
- Zwitterionic structure minimizes metal binding and enzymatic interference.
- High purity (98.0%).
- White to off-white solid, water soluble.
- Available in 10 G and larger lab-scale pack sizes.
- Store at room temperature; keep under nitrogen and away from moisture; in solution store at -20°C to -80°C for long-term storage.
- Supplied with SDS and COA documentation.