Life Science Buffers
Balanced salt solutions and powders used for molecular, protein, nucleic acid, and cell biology applications including cell culture, electrophoresis, ELISA, and chromatography processes; available in a variety of formulations and grades.
Buffer solutions serve to keep pH (acidity or alkalinity) nearly constant in a variety of chemical and biological applications. For example, a bicarbonate buffer helps maintain the pH of blood, a buffered saline maintains cellular contents at a consistent pH level, and buffers help maintain a narrow pH range for enzymes to function correctly.
Most biological samples used in research are kept at a pH of about 7.4. Common biological buffers used for tissue culture include Dulbeccos phosphate buffered saline (PBS), Tris base, HEPES, MOPS, PIPES, and other formulations developed for specific cell lines or applications. Most buffers used in cell culture are also DNase-, RNase-, and protease-free.
The choice of buffer for a particular biological reaction or site depends on several factors:
- Temperature
- Desired or target pH
- Buffer toxicity (to the system)
- Buffer interactions with other system components
Indofine Chemical 1,3,5-Triphenyl Tetraz, 1 Gm
1,3,5-TRIPHENYL TETRAZOLIUM FORMAZAN, Products for Life Science, 531-52-2, 1 gm
Medchemexpress LLC 2-Morpholinoethanesulphonic acid hydrate | 1266615-59-1 | 99.9% | 50 G
MES hydrate is an amphoteric ion buffer with a buffering capacity ranging from pH 5.5-7.0. It is widely used in biochemistry and molecular biology experiments for applications such as cell culture, enzyme activity determination, electrophoresis, and protein studies.
- Provides effective buffering capacity at approximately pH 6.15.
- Its pKa value changes with temperature, necessitating consideration for different experimental conditions.
- Exhibits good buffering properties in biological experiments.
- Shows poor affinity with metal ions, allowing its use in studies involving them.
Medchemexpress LLC MOPS (sodium salt) | 71119-22-7 | 99.8% | 50 G
MOPS sodium salt is a buffering agent used in biological applications. It helps maintain the pH of mammalian cell culture media and significantly improves the thermal stability of BSA. This product is also suitable for the preparation of keratinocyte and microbial culture media.
- Maintains pH of mammalian cell culture media
- Enhances thermal stability of BSA
- Suitable for preparing keratinocyte culture medium
- Suitable for preparing microbial culture medium
- Can be used in co-culture of keratinocytes and microorganisms
Apexbio Technology LLC HEPES Sodium salt 75277-39-3 250g
HEPES Sodium salt (CAS 75277-39-3) is a zwitterionic buffering agent designed to stabilize hydrogen ion concentration and maintain physiological pH (6 8 8 2) in biological systems thereby supporting cellular function and biomolecular interactions HEPES Sodium salt exerts its biological activity primarily through non-volatile amphoteric chemical buffering In in vitro and cell-based settings HEPES Sodium salt supports cell viability and experimental consistency by minimizing pH fluctuations Based on these properties HEPES Sodium salt holds research potential in cell-based assays maintenance of cultured mammalian cells controlled in vitro experiments studies of lysosomal biogenesis and investigations of cellular metabolic mechanisms
FUJIFILM BIOSCIENCES INC RPMI Medium 1640 1X HEPES
RPMI Medium 1640 1X HEPES w/oL-Glutamine is designed for growth of humanLeukemia cells in either monolayers or suspension and is useful for a wide variety of suspension and monolayer cultures as well as for hybridomas
![Research Products International Corp CAPS [3-(Cyclohexylamino)-1-propanesulphonic acid], 100 Grams](https://assets.fishersci.com/TFS-Assets/CCG/product-images/VN00006095-C30060-100.0.JPG-250.jpg)
Research Products International Corp CAPS [3-(Cyclohexylamino)-1-propanesulphonic acid], 100 Grams
pKa (25oC): 10.4. The working pH range in aqueous solution is 9.7-11.1
![Medchemexpress LLC (3-{[Tris(hydroxymethyl)methyl]amino}propane-1-sulfonic acid) | 29915-38-6 | 98.0% | 50 G](https://assets.fishersci.com/TFS-Assets/CCG/product-images/5000409966-logo.png-250.jpg){kind=logo}
Medchemexpress LLC (3-{[Tris(hydroxymethyl)methyl]amino}propane-1-sulfonic acid) | 29915-38-6 | 98.0% | 50 G
TAPS is an aminosulfonate-based lysozyme stabilizer and connexin channel inhibitor. It helps maintain the native structure of lysozyme in aqueous solution at pH 7.0 and protects it from heat-induced denaturation. TAPS is also considered a critical compound for investigating the structure and function of connexin channels.
- Maintains native structure of lysozyme in aqueous solution at pH 7.0
- Protects lysozyme from heat-induced denaturation
- Directly inhibits heteromeric connexin 32/connexin 26 channels (Cx32/Cx26)
- Reduces connexin channel-mediated solute exchange in a recombinant liposome system
- Critical compound for investigating connexin channel structure and function
Medchemexpress LLC 4-morpholineethanesulfonic acid, sodium salt (1:1) | 71119-23-8 | 98.0% | 10 G
MES sodium salt (2-Morpholinoethanesulphonic acid) is an amphoteric ion buffer with a buffering capacity ranging from pH 5.5-7.0. It is classified as a Good's buffer and is widely employed in biochemistry and molecular biology experiments, including cell culture, enzyme activity determination, electrophoresis, and protein studies.
- Amphoteric ion buffer with buffer capacity ranging from pH 5.5-7.0.
- Good's buffer.
- Widely used in biochemistry and molecular biology experiments.
- Provides effective buffering capacity around pH 6.15.
- pKa value changes with temperature, which should be considered for experiments at different temperatures.
- Shows good buffering properties in biological experiments.
- Exhibits poor affinity with metal ions (binding constants at 20 °C and 0.1 M: Mg2+, 0.8; Ca2+, 0.8; Mn2+, 1.7), making it suitable for studies involving metal ions.
Boston Bioproducts Inc MOPS Buffer (1 M, pH 7.0) - 500ml
The MOPS Buffer [3- (N-morpholino)propanesulfonic acid buffer] is a versatile zwitterionic Good's buffer used in biological research. With a pKa of 7.20, MOPS is an excellent buffer for many biological systems at near-neutral pH, and maintains a pH range from 6.5 to 7.9. It is often used as a buffering agent in biology and biochemistry, and is recommended for polyacrylamide gel electrophoresis, as a running buffer, in separating RNA in agarose gels, and for protein purification chromatography.
Medchemexpress LLC MOPSO sodium | 79803-73-9 | 97.0% | 247.24 | C7H14NNaO5S | 500 G
MOPSO sodium is the sodium salt of MOPSO, a zwitterionic buffering agent used in biochemical and molecular biology applications to prepare buffer solutions.
- Used for preparation of buffer solutions.
- Zwitterionic buffering agent; effective pH range typically near physiological pH.
- CAS number 79803-73-9.
- Chemical formula C7H14NNaO5S; molecular weight 247.24.
- Purity approximately 97.0% and available in 500 g packages.
Sigma Aldrich Fine Chemicals Biosciences MOPS sodium salt BioPerfor500G
MOPS sodium salt BioPerfor500G
Research Products International Corp TAPS, 100 G
pKa(25°:C): 8.4. The working pH range in aqueous solution is 7.7-9.1.