Life Science Buffers
Balanced salt solutions and powders used for molecular, protein, nucleic acid, and cell biology applications including cell culture, electrophoresis, ELISA, and chromatography processes; available in a variety of formulations and grades.
Buffer solutions serve to keep pH (acidity or alkalinity) nearly constant in a variety of chemical and biological applications. For example, a bicarbonate buffer helps maintain the pH of blood, a buffered saline maintains cellular contents at a consistent pH level, and buffers help maintain a narrow pH range for enzymes to function correctly.
Most biological samples used in research are kept at a pH of about 7.4. Common biological buffers used for tissue culture include Dulbeccos phosphate buffered saline (PBS), Tris base, HEPES, MOPS, PIPES, and other formulations developed for specific cell lines or applications. Most buffers used in cell culture are also DNase-, RNase-, and protease-free.
The choice of buffer for a particular biological reaction or site depends on several factors:
- Temperature
- Desired or target pH
- Buffer toxicity (to the system)
- Buffer interactions with other system components
Alkali Scientific CellPro™ DPBS 10X, 500ml
Balanced salt solution used for multiple cell culture applications, (liquid form). Certificate of Analysis available electronically. Standard stock items from 100 mL to 200 L Volumes up to 1000 L available as made to order Not for diagnostic or therapeutic use. For making isotonic solutions for cell or tissue rinsing, cell dilution, reagent prep, transportation etc. where maintenance of cell integrity is required for further manufacturing or research use.
Quality Biological Inc Tris HCl (2 M) pH 8.8 500mL
Tris solutions are formulated into working concentrations for preparing the stacking and resolving portions of native or SDS-PAGE gels using discontinuous buffer systems according to Laemmli (1970) or Ornstein and Davis (1959). Tris HCl Buffer is compatible with many enzymes in molecular biology (e.g. DNA modifying enzymes). Use the Tris-HCl 0.5 M (pH 6.8) buffer for stacking gels. Use Tris-HCl 1.5 M (pH 8.8) buffer for resolving gels. Use Tris-HCl 1M pH 7.0-7.5 for DEAE Dextran-mediated transfection of non-adherent and adherent cells.Manufactured in an ISO 90012015 certified facility.
Research Products International Corp PIPES Sodium Salt 250 G
pKa (25°C): 6.8
The working pH range in aqueous solution is 6.5-7.5
Research Products International Corp PBS 200ml Tablets 500 Tab
PBS, 200ml Tablets refer to pre-weighed tablets used to prepare a specific volume of PBS (Phosphate Buffered Saline) solution.
The buffer tablets are commonly used buffer solution in biological and biochemical research. PBS serves as a versatile buffer for various applications, including cell culture, immunohistochemistry, protein and nucleic acid research, and diagnostic assays. It helps maintain a stable pH and osmolarity, providing optimal conditions for biological reactions and preserving the integrity of cells and biomolecules.
The PBS, 200ml Tablets are designed to simplify the preparation process of PBS solutions. Each tablet contains pre-determined amounts of sodium chloride, sodium phosphate dibasic, and potassium phosphate monobasic. By using these tablets, researchers can save time and ensure accuracy by eliminating the need to weigh and measure individual components.
Alkali Scientific CellPro™ Tris Buffered Saline 10X, pH 7.4, 1L
Alkali Scientific™ CellPro™ Tris solution offers sterile and filtered Tris buffer for various laboratory applications. This high-quality solution is meticulously prepared to ensure purity and consistency, meeting the stringent requirements of scientific research and experimentation. With its sterile formulation, it minimizes the risk of contamination, making it suitable for a wide range of cell culture and molecular biology procedures. The filtered nature of the solution ensures clarity and reliability in experimental results, providing researchers with confidence in their findings. Whether used in protein biochemistry, DNA electrophoresis, or other molecular biology techniques, CellPro™ Tris solution delivers reliable performance and consistent results, contributing to the success of laboratory workflows.
Medchemexpress LLC 20 mM HEPES, 150 mM NaCl, 240 mM Sucrose, pH 7.2 buffer | 10 ML
This physiological buffer is composed of 20 mM HEPES, 150 mM NaCl, and 240 mM Sucrose, meticulously adjusted to a pH of 7.2. It is specifically designed for the resuspension of human erythrocyte ghost preparations.
- Physiological buffer
- Composed of 20 mM HEPES, 150 mM NaCl, and 240 mM Sucrose
- Adjusted to pH 7.2
- Used for resuspending human erythrocyte ghost preparations
Alkali Scientific CellPro™ 1M HEPES pH 7.5 (Adjusted with KOH) 1000mL
CellPro™ 1M HEPES pH 7.5 (Adjusted with KOH) 1000mL
Alkali Scientific CellPro™ Tris HCL, 1M pH 6.8, 500mL
Alkali Scientific™ CellPro™ Tris solution offers sterile and filtered Tris buffer for various laboratory applications. This high-quality solution is meticulously prepared to ensure purity and consistency, meeting the stringent requirements of scientific research and experimentation. With its sterile formulation, it minimizes the risk of contamination, making it suitable for a wide range of cell culture and molecular biology procedures. The filtered nature of the solution ensures clarity and reliability in experimental results, providing researchers with confidence in their findings. Whether used in protein biochemistry, DNA electrophoresis, or other molecular biology techniques, CellPro™ Tris solution delivers reliable performance and consistent results, contributing to the success of laboratory workflows.
Alkali Scientific CellPro™ HEPES Buffer, 0.5M, pH7.0, 125 ml bottle
CellPro™ HEPES Buffer, 0.5M, pH7.0, 125 ml bottle
Alkali Scientific CellPro™ PBS, 10X, pH 7.4, 2000mL Cube
Alkali Scientific™ CellPro™ PBS (Phosphate Buffered Saline) provides researchers with a reliable and consistent solution essential for various molecular and cellular biology applications. This meticulously prepared buffer is formulated to maintain a stable pH and osmolarity, ensuring optimal conditions for cell culture, protein assays, immunohistochemistry, and many other biological techniques. With its phosphate-based formulation, CellPro™ PBS offers excellent buffering capacity, making it suitable for maintaining the physiological pH of biological samples and preserving their integrity. Whether used for cell washing, sample dilution, or buffer exchange, CellPro™ PBS guarantees exceptional performance and reproducibility in every experiment. Trust in the quality and reliability of CellPro™ PBS to support your research needs and enable precise and consistent results.
Alkali Scientific CellPro™ PBS Buffer, 1X, p.H 7.2, 1L, 1 Liter
CellPro™ PBS Buffer, 1X pH 7.2, 1 Liter Phosphate-buffered saline (PBS) is a balanced salt solution that is used for a variety of cell culture applications, such as washing cells before dissociation, transporting cells or tissue samples, diluting cells for counting, and preparing reagents. PBS is formulated without calcium and magnesium for rinsing chelators from the culture before cell dissociation. Concentrated forms of Gibco™ PBS may require pH adjustment at the time of dilution.