Life Science Buffers
Balanced salt solutions and powders used for molecular, protein, nucleic acid, and cell biology applications including cell culture, electrophoresis, ELISA, and chromatography processes; available in a variety of formulations and grades.
Buffer solutions serve to keep pH (acidity or alkalinity) nearly constant in a variety of chemical and biological applications. For example, a bicarbonate buffer helps maintain the pH of blood, a buffered saline maintains cellular contents at a consistent pH level, and buffers help maintain a narrow pH range for enzymes to function correctly.
Most biological samples used in research are kept at a pH of about 7.4. Common biological buffers used for tissue culture include Dulbeccos phosphate buffered saline (PBS), Tris base, HEPES, MOPS, PIPES, and other formulations developed for specific cell lines or applications. Most buffers used in cell culture are also DNase-, RNase-, and protease-free.
The choice of buffer for a particular biological reaction or site depends on several factors:
- Temperature
- Desired or target pH
- Buffer toxicity (to the system)
- Buffer interactions with other system components
![Alkali Scientific DPBS 1X Solution [Dulbecco's Phosphate Buffered Saline 1X Sterile Solution], 1 L](https://assets.fishersci.com/TFS-Assets/CCG/product-images/5000660396.jpg-250.jpg)
Alkali Scientific DPBS 1X Solution [Dulbecco's Phosphate Buffered Saline 1X Sterile Solution], 1 L
DPBS 1X (Dulbecco's Phosphate Buffered Saline) is a sterile, ready-to-use buffered saline solution widely used in cell culture, molecular biology, and microbiology applications. This 1L solution is designed to maintain the osmotic balance and pH of biological samples, ensuring cell viability during laboratory procedures. DPBS 1X is commonly used for washing cells, preparing tissues for experiments, and diluting reagents. It is a standard medium in laboratories that require isotonic conditions to maintain the physiological state of cells and tissues during handling. The 1L size provides ample supply for large-scale cell culture and laboratory operations.
Apexbio Technology LLC Annexin V-Cy5/PI Apoptosis Kit 20assays
Annexin V is a Ca2 -dependent phospholipid-binding protein that specifically binds to phosphatidylserine (PS) Under normal conditions PS resides on the inner side of the cell membrane lipid bilayer However in early apoptosis PS translocates from the inner leaflet of the plasma membrane to the outer surface becoming exposed externally Annexin V exhibits high binding affinity for PS and when conjugated with fluorescent molecules (Cy5) it serves as a fluorescent probe to detect apoptosis using flow cytometry or fluorescence microscopy The staining procedure requires only 10 20 minutes PI specifically binds to double-stranded DNA to produce red fluorescence it is normally impermeant to intact cell membranes However during late-stage apoptosis or in dead cells PI penetrates the membrane to stain nuclear DNA Combined Annexin V-Cy5 and PI staining allows distinction between cells at various stages of apoptosis and dead cells
Miltenyi Biotec, Inc. CliniMACS PBS/EDTA Buffer 3 x 1L, HUD
Suitable for cell processing and cell separation in combination with the CliniMACS System.
This product is subject to the following: Miltenyi Biotec, Inc. Terms and Conditions of Sale For Products and Services
Biotium Fixation Permeabilization Other Buffers Buffers Accessories and Background Reducers Fixation Permeabilization and Other Buffers Chemical Reagents 50/PK
PBS (phosphate-buffered saline) buffer powder packet for preparing 2 L of 1X PBS buffer Box includes 50 packets
Biotang Inc Sterile PBS, 500 ml: 10 mM Sodium phosphate dibasic, 2 mM potassium phosphate monobasic, 2.7 mM potassium chloride, 137 mM sodium chloride, pH 7.4 +/- 0.2. Prepared in 18.2 megohms water. 0.22-micron filtered and/or autoclaved. BM-220S
Sterile PBS, 500 ml: 10 mM Sodium phosphate dibasic, 2 mM potassium phosphate monobasic, 2.7 mM potassium chloride, 137 mM sodium chloride, pH 7.4 +/- 0.2. Prepared in 18.2 megohms water. 0.22-micron filtered and/or autoclaved. BM-220S.
Teknova 1X PBS-TBN Buffer with 0.05% Tween, 1% BSA, 0.1% Sodium Azide. 1000mL, Sterile. 4 pack.
150mM Sodium Chloride, 50mM Monosodium Phosphate,1.00% Bovine Serum Albumin, 0.10% Sodium Azide,0.05% Tween-20 (v/v)
Medchemexpress LLC N,N-Bis(2-hydroxyethyl)-2-aminoethanesulfonic acid | 10191-18-1 | 98.0% | 500 G
BES is a secondary standard biochemical buffer used in diagnostic testing. It offers a stable pH environment for various applications.
- Effective pH range of 6.4 to 7.8
- pKa of 7.15 at 20 °C
- Suitable for diagnostic testing areas
FUJIFILM BIOSCIENCES INC RPMI Medium 1640 1X HEPES
RPMI Medium 1640 1X HEPES w/oL-Glutamine is designed for growth of humanLeukemia cells in either monolayers or suspension and is useful for a wide variety of suspension and monolayer cultures as well as for hybridomas
Medchemexpress LLC 2-Morpholinoethanesulphonic acid hydrate | 1266615-59-1 | 99.9% | 50 G
MES hydrate is an amphoteric ion buffer with a buffering capacity ranging from pH 5.5-7.0. It is widely used in biochemistry and molecular biology experiments for applications such as cell culture, enzyme activity determination, electrophoresis, and protein studies.
- Provides effective buffering capacity at approximately pH 6.15.
- Its pKa value changes with temperature, necessitating consideration for different experimental conditions.
- Exhibits good buffering properties in biological experiments.
- Shows poor affinity with metal ions, allowing its use in studies involving them.
Selleck Chemical LLC HEPES
HEPES is a nonvolatile zwitterionic chemical buffering agent which is broadly applied in cell culture HEPES is effective at pH 6 8 to 8 2 HEPES is also a potent inducer of lysosome biogenesis
![Alkali Scientific MOPS [3 (N morpholino) propanesulfonic acid], 250 G](https://assets.fishersci.com/TFS-Assets/CCG/product-images/5000660136.jpg-250.jpg)
Alkali Scientific MOPS [3 (N morpholino) propanesulfonic acid], 250 G
MOPS (3-(N-morpholino)propane-1-sulfonic acid) is a biochemical reagent designed for maintaining pH stability in a variety of laboratory procedures. This 250g package of MOPS is ideal for preparing moderate quantities of buffer solutions used in research, especially in molecular biology, protein chemistry, and biochemistry. MOPS is commonly employed in processes that require precise pH control, such as protein purification, enzyme assays, and electrophoresis. It is particularly effective in the pH range of 6.5 to 7.9 and is widely used to stabilize nucleic acids, proteins, and other biological molecules in experimental conditions.
![Alkali Scientific HEPES, Sodium Salt [N (2 Hydroxyethyl) piperazine N' (2 ethanesulfonic acid)], 5 Kg](https://assets.fishersci.com/TFS-Assets/CCG/product-images/5000659912.jpg-250.jpg)
Alkali Scientific HEPES, Sodium Salt [N (2 Hydroxyethyl) piperazine N' (2 ethanesulfonic acid)], 5 Kg
HEPES, Sodium Salt is a versatile biochemical reagent used to maintain a stable pH in biological and chemical applications. This 5kg package is ideal for large-scale laboratory use, providing ample quantities for high-volume buffer preparation. HEPES sodium salt is effective in the pH range of 6.8–8.2, making it suitable for a wide range of applications including cell culture, protein analysis, and enzymatic reactions. Its high buffering capacity and low toxicity to cells make it a reliable reagent for maintaining physiological pH during extended experiments. This bulk size ensures that laboratories can perform large-scale biochemical and biological studies with consistent results.
![Alkali Scientific TAPS, [Tris(hydroxymethyl) 3 aminopropanesulfonic acid], Free Acid, 100 G](https://assets.fishersci.com/TFS-Assets/CCG/product-images/5000660483.jpg-250.jpg)
Alkali Scientific TAPS, [Tris(hydroxymethyl) 3 aminopropanesulfonic acid], Free Acid, 100 G
TAPS, also known as Tris(hydroxymethyl) aminopropanesulfonic acid, is a high-purity buffering agent widely used in laboratory applications. The free acid form of TAPS is essential for preparing stable and reliable buffers, especially in experiments that require tight control over pH. It is commonly used in biochemical, molecular biology, and clinical research applications. The 100g quantity of TAPS is suitable for preparing a variety of buffer solutions for electrophoresis, enzyme assays, and other analytical procedures. The high purity ensures that the reagent will not interfere with sensitive analytical reactions, making it a go-to choice for researchers seeking consistency and precision in their experiments.